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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1_Lamp1_HA_GFP11

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: HA-GFP11; location: lysosome.

Catalog number RDB18655
Resource name pcDNA3.1_Lamp1_HA_GFP11
Alternative name pMM104
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human lysosomal associated membrane protein 1 (Lamp1) N-terminal 417 a.a fused with HA-GFP11 at C-terminus.
Comment This clone contains a cDNA fragment corresponding to the 197nt to 1447nt of NM_005561.4. Known differences (at least) : T to C substitution at 552nt (V119A); T to C substitution at 705nt (L170P); C to A substitution at 752nt (R186, synonym).
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human LAMP1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
human LAMP1 Aequorea victoria GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Shirane, M. et al. Nature comm. 11(1): 4576, 2020)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shirane, M. et al. Nature comm. 11(1): 4576, 2020)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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          RDB18655 pcDNA3.1_Lamp1_HA_GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Shirane, M., Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation. Nat. Commun. 11 (1): 4576 (2020). PMID 32917905.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualizing multiple inter-organelle contact sites (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18655_B0Lnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, insert 5'
          Sequence file: RDB18655_B0Lna.seq check
          >18655_18655_B0Ln_3_CMV_Forward_E07_13_ABI24.ab1
              1 NNNNNNNNNN NNNNTNNNNG CNNNGGGCGG TANGCGTGTA CGGTGGGNNG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGCG CCACCATGGC GGCCCCCGGC AGCGCCCGGC
            181 GACCCCTGCT GCTGCTACTG CTGTTGCTGC TGCTCGGCCT CATGCATTGT GCGTCAGCAG
            241 CAATGTTTAT GGTGAAAAAT GGCAACGGGA CCGCGTGCAT AATGGCCAAC TTCTCTGCTG
            301 CCTTCTCAGT GAACTACGAC ACCAAGAGTG GCCCTAAGAA CATGACCTTT GACCTGCCAT
            361 CAGATGCCAC AGTGGTGCTC AACCGCAGCT CCTGTGGAAA AGAGAACACT TCTGACCCCA
            421 GTCTCGTGAT TGCTTTTGGA AGAGGACATA CACTCACTCT CAATTTCACG AGAAATGCAA
            481 CACGTTACAG CGTCCAGCTC ATGAGTTTTG CTTATAACTT GTCAGACACA CACCTTTTCC
            541 CCAATGCGAG CTCCAAAGAA ATCAAGACTG TGGAATCTAT AACTGACATC AGGGCAGANN
            601 NNNNTNNNNN ATANNNNNNT GTTAGTGGNN NNNANNTCCA CATGANNNNN NTGACCGNAA
            661 ACGCCCCATG ATGCCACCAT CCAGGNNNTA CCTTTNNNNC ANNANCTTCN NCNNGNGANA
          721 AGACACGCTG TNNNNNNNAC ANGGCNNNNN NNCNNNNNNN NNN
          //
          Primer: bGH_rev3
          Region: BGH pA, GFP11, HA, insert 3'
          Sequence file: RDB18655_B0Lnb.seq check
          >18655_18655_B0Ln_3_bGH_rev3_F07_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGNNN NNGGGCANCA ANAGATGGCT GGCAACNAGA NNGCACAGTC
             61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGATTATG TAATCCCAGC AGCATTTACG
            121 TACTCATGAA GGACCATGTG GTCACGACTT GTTGATCCGC CACCAGACCC TCCATCGGCG
            181 GCCGCGCCGC CGGAGCCAGC GTAATCTGGA ACATCGTATG GGTAGAATTC GATAGTCTGG
            241 TAGCCTGCGT GACTCCTCTT CCTGCCGACG AGGTAGGCGA TGAGGACGAT GAGGACCAGC
            301 CCCGCCAGGG CACCACCCAC AGCGATGGGG ATCAGCATGC TGTTCTCGTC CAGCAGACAC
            361 TCCTCCACAG AGCCAAACTG GCCACCTTCC ACCTTGAAAG CCTGGACCCA CACTTTGAAT
            421 ATATTGACTG AAAACGCCTT CNTGACACGG ACGTGCTCCT CCGCGTTGCA CTTGTAGGAA
            481 TTGCCGACTG TGGCCTGCAN NGCTCGCANG GAGCCGTTGG CAGCTTTANN NGCAGGGTCT
            541 CTGGCGTCAN GAAGAATTGN NNTCNNNTGG ANTCCTTGNN NGAAAAACCN GGCTAANNNN
          601 NNNNNN
          //
          Primer: SV40pro_ori_F
          Region: NeoR
          Sequence file: RDB18655_B0Lnc.seq check
          >18655_18655_B0Ln_3_SV40pro_ori_F_C02_08_ABI24.ab1
              1 NNNNNNNNGN NCNNNNNNTT NNCAAAAAGC TCCCGGGAGC TTGNATATCC ATTTTCGGAT
             61 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
            121 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
            181 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
            241 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
            301 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
            361 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
            421 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
            481 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
            541 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
            601 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGN TCTCGTCGTG ACCCATGGCG
            661 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG
            721 GCCGGCTGGG TGTGNCGGAN CGCTATCANG ACATAGCGTT GGCTACNCGT GATATTGCTG
            781 AAGAGCTTGG CGGCNAATGG GCTGACCGCT TCCTCNNGCT TTACNGTATC NCCGCTCCCN
            841 ANTNNCANCG CATCGCCNNC NATCGCCNNC TTGNNGANTN NNNCNGANCG GGNNNNNGGG
          901 GNNCGAAANG NNNNANNNAG CNNNNNCNNN NNNNNCNNTC NNNANNNTNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Shirane, M., Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation. Nat. Commun. 11 (1): 4576 (2020). PMID 32917905.

          2022.08.11

          GNP_filter3_RDBDEP_html_220808.pl