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pcDNA3.1_Lamp1_HA_GFP11 (#RDB18655)

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: HA-GFP11; location: lysosome.

Alternative name pMM104
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human lysosomal associated membrane protein 1 (Lamp1) N-terminal 417 a.a fused with HA-GFP11 at C-terminus.
Comment This clone contains a cDNA fragment corresponding to the 197nt to 1447nt of NM_005561.4. Known differences (at least) : T to C substitution at 552nt (V119A); T to C substitution at 705nt (L170P); C to A substitution at 752nt (R186, synonym).
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human LAMP1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB18655hts01.seq checkAssembled from experimentally sequenced data.
Publication Shirane, M., Nat. Commun. 11 (1): 4576 (2020). [link to RRC of NBRP]
Test sheet RDB18655_B2Drp1-2.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Shirane, M. et al. Nature comm. 11(1): 4576, 2020)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shirane, M. et al. Nature comm. 11(1): 4576, 2020)。

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RDB18655 pcDNA3.1_Lamp1_HA_GFP11 DNA solution


How to cite this biological resource

Materials & Methods section:

The pcDNA3.1_Lamp1_HA_GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18655).

Reference section:

Shirane, M., Wada, M., Morita, K., Hayashi, N., Kunimatsu, R., Matsumoto, Y., Matsuzaki, F., Nakatsumi, H., Ohta, K., Tamura, Y., Nakayama, K.I., Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation. Nat. Commun. 11 (1): 4576 (2020). PMID 32917905. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Shirane, M., Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation. Nat. Commun. 11 (1): 4576 (2020). PMID 32917905. [link to RRC of NBRP]