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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1_Lamp1_HA_GFP11

The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human Lamp1 N-terminal 417 a.a fused with HA-GFP11 at C-terminus.

Catalog number RDB18655
Resource name pcDNA3.1_Lamp1_HA_GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human lysosomal associated membrane protein 1 (Lamp1) N-terminal 417 a.a fused with HA-GFP11 at C-terminus.
This clone contains a cDNA fragment corresponding to the 197nt to 1447nt of NM_005561.4. Known differences (at least) : T to C substitution at 552nt (V119A); T to C substitution at 705nt (L170P); C to A substitution at 752nt (R186, synonym).
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human LAMP1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
 
Other clones in our bank human LAMP1 (NCBI Gene 3916) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Shirane, M. et al. Nature comm. 11(1): 4576, 2020)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
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RDB18655 pcDNA3.1_Lamp1_HA_GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Shirane, M., Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation. Nat. Commun. 11 (1): 4576 (2020). PMID 32917905.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18655_B0Lnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB18655_B0Lna.seq check
>18655_18655_B0Ln_3_CMV_Forward_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNNTNNNNG CNNNGGGCGG TANGCGTGTA CGGTGGGNNG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGCG CCACCATGGC GGCCCCCGGC AGCGCCCGGC
  181 GACCCCTGCT GCTGCTACTG CTGTTGCTGC TGCTCGGCCT CATGCATTGT GCGTCAGCAG
  241 CAATGTTTAT GGTGAAAAAT GGCAACGGGA CCGCGTGCAT AATGGCCAAC TTCTCTGCTG
  301 CCTTCTCAGT GAACTACGAC ACCAAGAGTG GCCCTAAGAA CATGACCTTT GACCTGCCAT
  361 CAGATGCCAC AGTGGTGCTC AACCGCAGCT CCTGTGGAAA AGAGAACACT TCTGACCCCA
  421 GTCTCGTGAT TGCTTTTGGA AGAGGACATA CACTCACTCT CAATTTCACG AGAAATGCAA
  481 CACGTTACAG CGTCCAGCTC ATGAGTTTTG CTTATAACTT GTCAGACACA CACCTTTTCC
  541 CCAATGCGAG CTCCAAAGAA ATCAAGACTG TGGAATCTAT AACTGACATC AGGGCAGANN
  601 NNNNTNNNNN ATANNNNNNT GTTAGTGGNN NNNANNTCCA CATGANNNNN NTGACCGNAA
  661 ACGCCCCATG ATGCCACCAT CCAGGNNNTA CCTTTNNNNC ANNANCTTCN NCNNGNGANA
721 AGACACGCTG TNNNNNNNAC ANGGCNNNNN NNCNNNNNNN NNN
//
Primer: bGH_rev3
Region: BGH pA, GFP11, HA, insert 3'
Sequence file: RDB18655_B0Lnb.seq check
>18655_18655_B0Ln_3_bGH_rev3_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NNGGGCANCA ANAGATGGCT GGCAACNAGA NNGCACAGTC
   61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGATTATG TAATCCCAGC AGCATTTACG
  121 TACTCATGAA GGACCATGTG GTCACGACTT GTTGATCCGC CACCAGACCC TCCATCGGCG
  181 GCCGCGCCGC CGGAGCCAGC GTAATCTGGA ACATCGTATG GGTAGAATTC GATAGTCTGG
  241 TAGCCTGCGT GACTCCTCTT CCTGCCGACG AGGTAGGCGA TGAGGACGAT GAGGACCAGC
  301 CCCGCCAGGG CACCACCCAC AGCGATGGGG ATCAGCATGC TGTTCTCGTC CAGCAGACAC
  361 TCCTCCACAG AGCCAAACTG GCCACCTTCC ACCTTGAAAG CCTGGACCCA CACTTTGAAT
  421 ATATTGACTG AAAACGCCTT CNTGACACGG ACGTGCTCCT CCGCGTTGCA CTTGTAGGAA
  481 TTGCCGACTG TGGCCTGCAN NGCTCGCANG GAGCCGTTGG CAGCTTTANN NGCAGGGTCT
  541 CTGGCGTCAN GAAGAATTGN NNTCNNNTGG ANTCCTTGNN NGAAAAACCN GGCTAANNNN
601 NNNNNN
//
Primer: SV40pro_ori_F
Region: NeoR
Sequence file: RDB18655_B0Lnc.seq check
>18655_18655_B0Ln_3_SV40pro_ori_F_C02_08_ABI24.ab1
    1 NNNNNNNNGN NCNNNNNNTT NNCAAAAAGC TCCCGGGAGC TTGNATATCC ATTTTCGGAT
   61 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
  121 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
  181 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
  241 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
  301 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
  361 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
  421 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
  481 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
  541 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
  601 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGN TCTCGTCGTG ACCCATGGCG
  661 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG
  721 GCCGGCTGGG TGTGNCGGAN CGCTATCANG ACATAGCGTT GGCTACNCGT GATATTGCTG
  781 AAGAGCTTGG CGGCNAATGG GCTGACCGCT TCCTCNNGCT TTACNGTATC NCCGCTCCCN
  841 ANTNNCANCG CATCGCCNNC NATCGCCNNC TTGNNGANTN NNNCNGANCG GGNNNNNGGG
901 GNNCGAAANG NNNNANNNAG CNNNNNCNNN NNNNNCNNTC NNNANNNTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shirane, M., Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation. Nat. Commun. 11 (1): 4576 (2020). PMID 32917905.

2021.07.10

GNP_filter3_RDBDEP_html_210618.pl