The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: HA-GFP11; location: lysosome.
Alternative name | pMM104 |
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Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human lysosomal associated membrane protein 1 (Lamp1) N-terminal 417 a.a fused with HA-GFP11 at C-terminus. |
Comment | This clone contains a cDNA fragment corresponding to the 197nt to 1447nt of NM_005561.4. Known differences (at least) : T to C substitution at 552nt (V119A); T to C substitution at 705nt (L170P); C to A substitution at 752nt (R186, synonym). |
Vector backbone | pcDNA3.1 (plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Ampicillin |
Gene/insert name | human LAMP1 cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Tamura, Yasushi | |
Sequence (full) | RDB18655hts01.seq ![]() |
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Publication | Shirane, M., Nat. Commun. 11 (1): 4576 (2020). [link to RRC of NBRP] |
Test sheet | RDB18655_B2Drp1-2.pdf ![]() |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Shirane, M. et al. Nature comm. 11(1): 4576, 2020) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shirane, M. et al. Nature comm. 11(1): 4576, 2020)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18655 | pcDNA3.1_Lamp1_HA_GFP11 | DNA solution |
Materials & Methods section:
The pcDNA3.1_Lamp1_HA_GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18655). |
Reference section:
Shirane, M., Wada, M., Morita, K., Hayashi, N., Kunimatsu, R., Matsumoto, Y., Matsuzaki, F., Nakatsumi, H., Ohta, K., Tamura, Y., Nakayama, K.I., Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation. Nat. Commun. 11 (1): 4576 (2020). PMID 32917905. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Shirane, M., Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation. Nat. Commun. 11 (1): 4576 (2020). PMID 32917905. [link to RRC of NBRP] |
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