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pCW DNAseIIα-sfGFP-T2A-mCherry

Lentiviral expression vector of lysosomal-METRIQ (MEasurement of protein Transporting integrity by RatIo Quantification) probe.

Catalog number RDB18608
Resource name pCW DNAseIIα-sfGFP-T2A-mCherry
Alternative name Lysosomal-METRIQ
Clone info. Lentiviral expression vector of DNaseII alpha-sfGFP-T2A-mCherry for lysosomal-METRIQ (MEasurement of protein Transporting integrity by RatIo Quantification) probe, see Fig. 1 of Ishii, S. et al., Sci. Rep., 9 (1): 11635, 2019.
Comment For expression in mammalian cells, 1 to 3 microgram/mL doxycycline is required. Expression was confirmed by the depositor with immunofluorescence microscopy.
Vector backbone pCW57.1 (plasmid)
Size of vector backbone 9.4 kb
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell).
Gene/insert name human DNASE2 cDNA
Depositor|Developer Itakura, Eisuke |
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External Database
human DNASE2
          Reference sequence
            
           
          Sequence (full) RDB18608zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, S et al. Sci Rep. 9 (1), 11635, 2019)
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, S et al. Sci Rep. 9 (1), 11635, 2019)。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18608 pCW DNAseIIα-sfGFP-T2A-mCherry Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Ishii, S., Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Sci. Rep. 9 (1): 11635 (2019). PMID 31406169.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Ishii, S., Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Sci. Rep. 9 (1): 11635 (2019). PMID 31406169.

          2022.08.12

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