Retroviral expression vector of human TFEB tagged with superfolder GFP.
Clone info. | Retroviral expression vector of human transcription factor EB (TFEB) tagged with Superfolder GFP (sfGFP) at C-terminus. |
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Comment | Expression was confirmed by the depositor with immunofluorescence microscopy. |
Vector backbone | pMRX-IP (pMRX‐IRES‐puro) (plasmid) |
Selectable markers | Ampicillin (E. coli), Puromycin (mammalian cell). |
Gene/insert name | human TFEB cDNA |
Depositor|Developer | Itakura, Eisuke | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, S et al. Sci Rep. 9 (1), 11635, 2019) Additional terms and conditions: Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested. |
Remarks | Remember that you will be working with samples containing infectious virus. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, S et al. Sci Rep. 9 (1), 11635, 2019)。 MTAに書く付加的使用条件: pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB18607 | pMRX-IP TFEB-sfGFP | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pMRX-IP TFEB-sfGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18607). |
Reference section:
Ishii, S., Matsuura, A., Itakura, E., Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Sci. Rep. 9 (1): 11635 (2019). PMID 31406169. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Ishii, S., Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Sci. Rep. 9 (1): 11635 (2019). PMID 31406169. [link to RRC of NBRP] |
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