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KPNB1/Flag pcDNA (#RDB18584)

Expression vector of human KPNB1/importin-beta.


Drawn by SnapGene® software
Sequence (full) RDB18584hts01.seq
Publication Oguro, A., Sci. Rep. 9 (1): 15296 (2019). [link to RRC of NBRP]
Test sheet RDB18584_B0J9p1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of human karyopherin subunit beta 1 (KPNB1/importin-beta) tagged with 3x FLAG. Please note that the zeocin resistance gene has been replaced with the puromycin resistance gene.
Vector backbone 3xFlag-pcDNA4 (plasmid)
Growth conditions LB+Amp, 37oC
Gene/insert name Human KPNB1 cDNA
Depositor|Developer Imaoka, Susumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Oguro, A. and Imaoka, S. Sci. Rep. 9 (1), 15296, 2019)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Oguro, A. and Imaoka, S. Sci. Rep. 9 (1), 15296, 2019)。

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RDB18584 KPNB1/Flag pcDNA DNA solution

How to cite this biological resource

Materials & Methods section:

The KPNB1/Flag pcDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18584).

Reference section:

Oguro, A., Imaoka, S., Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-beta-dependent nuclear cargo transport. Sci. Rep. 9 (1): 15296 (2019). PMID 31653923. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Oguro, A., Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-beta-dependent nuclear cargo transport. Sci. Rep. 9 (1): 15296 (2019). PMID 31653923. [link to RRC of NBRP]