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pEF-FLAG-Rab34-S1A 

Expression vector of mouse Rab34, switch II region-swapping mutant.

Catalog number RDB18393
Resource name pEF-FLAG-Rab34-S1A 
Clone info. Expression vector of mouse Rab34, switch II region-swapping mutant, tagged with FLAG at N-terminus.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-BOS FLAG (a.k.a. pEF-FLAG) (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Ampicillin
Gene/insert name mouse Rab34 cDNA
Reference of nucleotide sequence AB232630 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. J. Biol. Chem. 277, 12432-12436, 2002; Oguchi, M. E. et al. J. Biol. Chem. in press (doi:10.1074/jbc.RA119.012233), 2020)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Ordering Please visit Information of Request for Distribution.[link] 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18393 pEF-FLAG-Rab34-S1A  Under QC test. Please contact us. DNA solution

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Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB18393pka01.seq

Original reference

original Oguchi, M.E., A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis. J. Biol. Chem. 295 (36): 12674-12685 (2020). PMID 32669361.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Oguchi, M.E., A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis. J. Biol. Chem. 295 (36): 12674-12685 (2020). PMID 32669361.

2021.01.31

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