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pMRX-bsr-EGFP-P2A-Rab34 

Retroviral expression vector of mouse Rab34.

Catalog number RDB18392
Resource name pMRX-bsr-EGFP-P2A-Rab34 
Clone info. Retroviral expression vector of mouse Rab34. An EGFP and Rab34 linked by self-cleaving P2A peptide.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-IRES-bsr-EGFP (plasmid)
Selectable markers Amp^r (E. coli), Blasticidin^r (mammalian cell).
Gene/insert name mouse Rab34 cDNA
Reference of nucleotide sequence AB232630 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. J. Biol. Chem. 277, 12432-12436, 2002; Oguchi, M. E. et al. J. Biol. Chem. in press (doi:10.1074/jbc.RA119.012233), 2020)

Additional terms and conditions for distribution Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18392 pMRX-bsr-EGFP-P2A-Rab34  Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB18392pka01.seq

Original reference

original Oguchi, M.E., A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis. J. Biol. Chem. 295 (36): 12674-12685 (2020). PMID 32669361.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Oguchi, M.E., A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis. J. Biol. Chem. 295 (36): 12674-12685 (2020). PMID 32669361.

2021.01.31

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