Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3-FLAG-Crb3ΔCter

Expression vector of human Crb3 mutant lacking unique sequence encoded by 3a or 3b at C‐terminus.

Catalog number RDB18381
Resource name pcDNA3-FLAG-Crb3ΔCter
Clone info. Expression vector of human crumbs cell polarity complex component 3 (Crb3) mutant lacking unique sequence encoded by 3a or 3b at C‐terminus (delta Cter) tagged with 2xFLAG inserted between Leu40 and Pro41., see Fig.S6C of Iioka, H. et. al, Int. J. Cance, 145 (10): 2740-2753, 2019.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli)
Gene/insert name human CRB3 cDNA
Depositor|Developer Iioka, Hidekazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Ordering Please visit Information of Request for Distribution.[link] 
Order form [Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18381 pcDNA3-FLAG-Crb3ΔCter Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB18381zzk01.seq

Original reference

original Iioka, H., Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma via the specific interaction with FGFR1. Int. J. Cancer 145 (10): 2740-2753 (2019). PMID 30980524.

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Iioka, H., Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma via the specific interaction with FGFR1. Int. J. Cancer 145 (10): 2740-2753 (2019). PMID 30980524.

2021.01.07

GNP_filter3_RDBDEP_html_201222.pl