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CoralHue™ humanized monomeric Umikinoko-Green 1 (phmUkG1-S1)
| Catalog number | RDB18307 |
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| Resource name | CoralHue(TM) humanized monomeric Umikinoko-Green 1 (phmUkG1-S1) |
| Alternative name | AM-V0164M |
| Clone info. | ・For subcloning. ・The codons are humanized. ・Monomeric Umikinoko-Green1 is brilliant green fluorescent protein and has extremely high pH stability. ・Ex/Em(nm): 483/499 |
| Vector backbone | S1 (plasmid) |
| Size of vector backbone | 2.7 kb |
| Selectable markers | Ampicillin |
| Gene/insert name | Sarcophyton sp. green fluorescent protein cDNA |
| Depositor|Developer | MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Tsutsui, H. et al., Nat. Methods 5 (8): 683-685, 2008) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA. |
|---|---|
| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ] [Bank Transfer Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB18307 | CoralHue(TM) humanized monomeric Umikinoko-Green 1 (phmUkG1-S1) | DNA solution |
icon below as well as clone information before placing your order.Electronic file
| Sequence (full) | RDB18307zzk01.seq provided by the depositor |
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| Remarks, protocol and/or map (png) | RDB18307_map.png
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Original reference
| original | Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Fluorescent protein resources (English text) |
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| Featured content | Fluorescent protein resources (Japanese text) |
| Featured content | CoralHue™ Fluorescent Protein (Japanese text) |
| Featured content | CoralHue™ Fluorescent Protein (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB18307_B0H4p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: lacIpro-B_F Region: MCS, insert 5' Sequence file: RDB18307_B0H4a.seq
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>D07039H4_B0H4_3_lacIpro-B_F_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGCN NCGNNNCNGT GNGCGNGGAA NCGGAAGAGC GCCCAATACG 61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC 121 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC 181 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA 241 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCGGGG 301 ATCCCCACCA TGGTGAGCGT GATCAAGGAG GAGATGAAGA TCAAGCTGCA CATGGAGGGC 361 AACGTGAACG GCCACGCCTT CGTGATCGAG GGCGACGGCA AGGGCAAGCC CTACGACGGC 421 ACCCAGACCC TGAACCTGAC CGTGAAGGAG GGCGCCCCCC TGCCCTTCAG CTACGACATC 481 CTGACCAACG CCTTCCAGTA CGGCAACCGC GCCTTCACCA AGTACCCCGC CGACATCCCC 541 GACTACTTCA AGCAGACCTT CCCCGAGGGC TACAGCTGGG AGCGCACCAT GAGCTACGAG 601 GACAACGCCA TCTGCAACGT GCGCAGCGAG ATCAGCATGG AGGGCGACTG CTTCATCTAC 661 AAGATCCGCT TCGACGGCAA GAACTTCCCC CCCAACGGCC CCGTGATGCA GAAGAAGANC 721 CTGAAGTGGG AGCCCAGCAC CGAGATGATG TACGTGCGCG ACGGCTTCCT GATGGGCGAC 781 GTGAACATGG CCCTGCTGCT GGANGGCGGC GGCCACCACC GCNGCGACTT CNAGANCAGC 841 TACAANGCCN ANAANGTGGN GCAGCTGCCC NACNCCCACA AGATCGACCA NNGCATCGAN 901 ANCCTGANNC ACGACCNCNN NTACNNNNAN GNNNAANCNN NACNNNNNNN CCNNNNNNNN 961 NN // |
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Primer: MBL_S1-rev1 Region: MCS, insert 3' Sequence file: RDB18307_B0H4b.seq
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>D07039H4_B0H4_3_MBL_S1-rev1_C09_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGGNGNN CGGNGCGGGC CTCTTCGCTA TTACGCCNGC 61 TGGCGAAAGG GGGNNGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT 121 CACGACGTTG TAAAACGACG GCCAGTGCCA AGCTTGCATG CCTGCAGTTA CTTGCTGGCC 181 TGGCTGGGCA GCAGGCTGTT GCGGGCCACG GCGTTCTCGT ACAGCTTCAC CTTGCTGTAG 241 TCGCGGTCGT GGCTCAGGAT CTCGATGCGG TGGTCGATCT TGTGGGCGTC GGGCAGCTGC 301 ACCACCTTCT TGGCCTTGTA GCTGGTCTTG AAGTCGCAGC GGTGGTGGCC GCCGCCCTCC 361 AGCAGCAGGG CCATGTTCAC GTCGCCCATC AGGAAGCCGT CGCGCACGTA CATCATCTCG 421 GTGCTGGGCT CCCACTTCAG GGTCTTCTTC TGCATCACGG GGCCGTTGGG GGGGAAGTTC 481 TTGCCGTCGA AGCGGATCTT GTAGATGAAG CAGTCGCCCT CCATGCTGAT CTCGCTGCGC 541 ACGTTGCAGA TGGCGTTGTC CTCGTAGCTC ATGGTGCGCT CCCAGCTGTA GCCCTCGGGG 601 AAGGTCTGCT TGAAGTAGTC GGGGATGTCG GCGGGGTACT TGGTGAAGGC GCGGTTGCCG 661 TACTGGAAGG CGTTGGTCAG GATGTCGTAG CTGAAGGGCA GGGGGGCGCC CTCCTTCACG 721 GTCAGGTTCA GGGTCTGGGT GCCGTCGTAN GGCTTGCCCT TGCCGTCGCC CTCGATCACG 781 AANGCGTGGC CGTTCACGTT GCCCTCCATG TGCAGCTTGA TCTTCATCTC CTCCTTGATC 841 ACGCTCACCA TGNNGGGGAN CCCCGGGNAN CNANCTCGAA NTCGTAATCA TGGNCATANC 901 NGTTTCCTGN GNGAAATNGN TATCNNCTCN NNNNNNCNCA CNANATACNA NNNNNNNNNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. |
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2021.08.13
GNP_filter3_RDBDEP_html_210618.pl