Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized monomeric Kusabira-Orange 2 (phmKO2-MNL) (#RDB18298)

CoralHue™ humanized monomeric Kusabira-Orange 2 (phmKO2-MNL)

Alternative name AM-V0140M
Clone info. ・MCS-Linker-Fluorescent protein/For mammalian expression of the fluorescent protein or its fusion.
・The codons are humanized.
・Monomeric Kusabira-Orange2 is mutant of Ornage fluorescent protein KO1 and has a feature of rapidly maturation.
・Ex/Em(nm): 551/565
Vector backbone MNL (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Fungia concinna orange fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18298zzk01.seq provided by the depositor
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18298 CoralHue(TM) humanized monomeric Kusabira-Orange 2 (phmKO2-MNL) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) humanized monomeric Kusabira-Orange 2 (phmKO2-MNL) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18298).

Reference section:

Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18298_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,MCS,Linker,insert 5'
Sequence file: RDB18298_B0H4a.seq check
>D07039G4_B0H4_4_CMV_Forward_B07_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNG NNNCNNATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCA TTGGATCCTC AGGTACCGGA
  121 ACTGCAGCAG AGAATTCGGG AAACTCGAGA ACAAAGCTTG GATCAGCGGC CGCCAATTCC
  181 GCTGACGGCG GCGGAGGATC GGGTGGTAGT GGTGGTTCAG GAGGAGGATC GACCCAAGGA
  241 ACCGGTATGG TGAGCGTGAT CAAGCCCGAG ATGAAGATGA GGTACTACAT GGACGGCTCC
  301 GTCAATGGGC ATGAGTTCAC AATCGAGGGT GAGGGCACAG GCAGACCTTA CGAGGGACAT
  361 CAGGAGATGA CACTGCGCGT CACAATGGCC GAGGGCGGGC CAATGCCTTT CGCCTTCGAC
  421 CTGGTGTCCC ACGTGTTCTG TTACGGCCAC AGAGTTTTTA CCAAGTACCC AGAAGAGATC
  481 CCAGACTATT TCAAGCAGGC CTTTCCTGAG GGCCTGTCCT GGGAGAGGTC CCTGGAGTTC
  541 GAGGACGGCG GCTCCGCCTC CGTGAGCGCC CACATCAGCC TGAGGGGCAA CACCTTCTAC
  601 CACAAGTCCA AGTTCACCGG CGTGAACTTC CCCGCCGACG GCCCCATCAT GCAGAACCAG
  661 AGCGTGGACT GGGAGCCCTC CACCGAGAAG ATCACCGCCA GCGACGGCGT GCTGAANGGC
  721 GACGTGACCA TGTACCTGAA GCTGGAGGGC GGCGGCAACC ACAAGTGCCA NATGAAGACC
  781 ACCTACNNNG CCGCNNNGGA GATCCTGGAG ATGCCCGGCN ACCACTACAT CNNCCACANG
  841 CTGGTGAGGA NNANCGANGG CAACATCACC NANCANGTGN ANGANGCCGN GGNCCNACNC
  901 NTAATCNNNN NGGGGNNNCN ACNCTANATC ATAANCNNCN NNANNNCNNT TGNNNAGGNT
  961 TTANNTGCNT TNNAAAAANC NNCCCACNNC NNCCCCCNGN ANCNNGNAAN CATAAAANNN
1021 AANGNNNNNN NNGNNNNNNN NNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert,Linker
Sequence file: RDB18298_B0H4b.seq check
>D07039G4_B0H4_4_SV40polyA-R2_B08_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTTAANN ACNACNATTG CATTCNTTTT ATGTTTCNGG
   61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCTAGAGTCG CGGCCCCCTC TAGATTAGGA GTGGGCCACG GCGTCCTCCA
  181 CCTGCTCGGT GATGTTGCCC TCGGTCTTCC TCACCAGCCT GTGGCCGATG TAGTGGTCGC
  241 CGGGCATCTC CAGGATCTCC TTGGCGGCCT TGTAGGTGGT CTTCATCTGG CACTTGTGGT
  301 TGCCGCCGCC CTCCAGCTTC AGGTACATGG TCACGTCGCC CTTCAGCACG CCGTCGCTGG
  361 CGGTGATCTT CTCGGTGGAG GGCTCCCAGT CCACGCTCTG GTTCTGCATG ATGGGGCCGT
  421 CGGCGGGGAA GTTCACGCCG GTGAACTTGG ACTTGTGGTA GAAGGTGTTG CCCCTCAGGC
  481 TGATGTGGGC GCTCACGGAG GCGGAGCCGC CGTCCTCGAA CTCCAGGGAC CTCTCCCAGG
  541 ACAGGCCCTC AGGAAAGGCC TGCTTGAAAT AGTCTGGGAT CTCTTCTGGG TACTTGGTAA
  601 AAACTCTGTG GCCGTAACAG AACACGTGGG ACACCAGGTC GAAGGCGAAA GGCATTGGCC
  661 CGCCCTCGGC CATTGTGACG CGCAGTGTCA TCTCCTGATG TCCCTCGTAA GGTCTGCCTG
  721 TGCCCTCACC CTCGATTGTG AACTCATGCC CATTGACGGA GCCGTCCATG TAGTACCTCA
  781 TCTTCATCTC GGGCTTGATC ACGCTCACCA TACCGGTTCC TTGGGTCGAT CCTCCTCCTG
  841 AACCACCACT ACCACCCGAT CCTCCGCCGC CGTCAGCGGA ATTGNNGGCC GCTGATNCNA
  901 GCTTTGTTCT CGAGTTTCCC NAATTCTCTG CTNCANTTCC GGNNNNNNAN GNTNCNANGN
961 NAGCNNNTNN NANGNNTCNC TNANCCNGCT CNGNNTNNTA TANNNNNNCN NNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB18298_B0H4c.seq check
>D07039G4_B0H4_4_SV40pro_ori_F_B09_06_ABI24.ab1
    1 NNNNNNNNNN NGNNGCCGCC TCGNCCTCTG ANCTATTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAGATCATC AAGAGACAGG ATGAGGATCG TTTCGCATGA
  121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT
  181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC
  241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG
  301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG
  361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC
  421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC
  481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG
  541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC
  601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG
  661 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC
  721 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT CANGACATAG
  781 CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGNGCTGAN CGCTTCCTCG
  841 TGCTTTACNG NATCGCCNCT CCCGATTCNC AGCGCATCGC CNTCTATCGC CTTCTTGANN
  901 ANTTCTTCTG ANCNGGNNNN NGGGGGTTCG AAATGANCNA NNNAGCGANG CCNNNNCTGN
961 CNTCNCNAGA TTTCNANTCC NNNNNCNNCN TTCTATGAAA NGNNNGGNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]