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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) dimeric Keima570 (pdKeima570-S1)

CoralHue™ dimeric Keima570 (pdKeima570-S1)

Catalog number RDB18294
Resource name CoralHue(TM) dimeric Keima570 (pdKeima570-S1)
Alternative name AM-V0121M
Clone info. ・For subcloning.
・Dimeric Keima570 exhibits an extremely large Stokes shift(130 nm) and is stable under usual aerobic conditions.
・Ex/Em(nm): 440/570
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank
External Database
Montipora. sp red fluorescent protein
            Reference sequence
              
             
            Sequence (full) RDB18294zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18294_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18294 CoralHue(TM) dimeric Keima570 (pdKeima570-S1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18294_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: MCS, insert 5'
            Sequence file: RDB18294_B0H4a.seq check
            >D07039F9_B0H4_1_lacIpro-B_F_D01_10_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGTGAGC GAGGAAGCGG AAGAGCGCCC
               61 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG
              121 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA
              181 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG
              241 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGAATTCG AGCTCGGTAC
              301 CCGGGGATCC CCACCATGGT GAGTGTGATC GCTAAACAAA TGACCTACAA GGTTTATATG
              361 TCAGGCACGG TCAATGGACA CTACTTTGAG GTCGAAGGCG ATGGAAAAGG AAAGCCTTAC
              421 GAGGGAGAGC AGACAGTAAA GCTCACTGTC ACCAAGGGTG GACCTCTGCC ATTTGCTTGG
              481 GATATATTAT CACCACTGAT GTGTTACGGA AGCATACCAT TCACCAAGTA CCCTGAAGAC
              541 ATCCCTGATT ATGTAAAGCA GTCATTCCCT GAGGGATATA CATGGGAGAG GACCATGAAC
              601 TTTGAAGATG GTGCAGTGTG TACTGTCAGC AATGATTCCA GCATCCAAGG CAACTGTTTC
              661 ATCTACNATG TCAAAATCTC TGGTACGAAC TTTCCTCCCA ATGGACCTGT TATGCAGAAG
              721 AAGANACAGG GCTGGGAACC CAGCACTGAN CGTCTCTTTG CACGAGATGN AATGCTGATA
              781 GGAAACGATT ATATGGCTCT GAAGTTGGAA NGANGTGGTC ACTATTTGTG TGAGTTCNAA
              841 TCTACTTACN ANGCAAAGAA NCCNGTGANG ATGCCNNGGN ATCACTATAT TGANNGCNAA
              901 CTGGATGTNN CCNGNCACNA CANGNNNTAC NCATCTGNTN ANCANNNNGA AATANNNNTT
            961 GNNNNNNNNN N
            //
            Primer: MBL_S1-rev1
            Region: MCS, insert 3'
            Sequence file: RDB18294_B0H4b.seq check
            >D07039F9_B0H4_1_MBL_S1-rev1_B11_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGNGCGGGC CTCTTCGCNA TTACGCCNGC
               61 TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT
              121 CACGACGTTG TAAAACGACG GCCAGTGCCA AGCTTGCATG CCTGCAGTTA ACCGAGCAAA
              181 GAGTGGCGTG CAATGGCTAT TTCACACTGC TCAACAGATG TGTAATCCCT GTTGTGACTG
              241 GTTACATCCA GTTTGCGGTC AATATAGTGA TACCCTGGCA TCCTCACAGG CTTCTTTGCC
              301 TTGTAAGTAG ATTTGAACTC ACACAAATAG TGACCACCTC CTTCCAACTT CAGAGCCATA
              361 TAATCGTTTC CTATCAGCAT TCCATCTCGT GCAAAGAGAC GCTCAGTGCT GGGTTCCCAG
              421 CCCTGTGTCT TCTTCTGCAT AACAGGTCCA TTGGGAGGAA AGTTCGTACC AGAGATTTTG
              481 ACATTGTAGA TGAAACAGTT GCCTTGGATG CTGGAATCAT TGCTGACAGT ACACACTGCA
              541 CCATCTTCAA AGTTCATGGT CCTCTCCCAT GTATATCCCT CAGGGAATGA CTGCTTTACA
              601 TNNNCNGGGA TGTCTTCAGG GTACTTGGTG AATGGTATGC TTCCGTAACA CATCAGTGGT
              661 GATAATATAT CCCAAGCAAA TGGCAGANGT CCACCCTTGG TGACAGTGAG CTTTACTGTC
              721 TGCTCTCCCT CGTAAGGCTT TCCTTTTCCA TCGCCTTCGA CCTCNAAAGT ANTGTCCATT
              781 GNNCGTGCCT GACATATAAA CCNTNNNNGT CATTTGTTTA NCGATCACAC TCACCATGGT
              841 GGGGATCCCC GGNTANCGAN CTCNAATTCN TAATCATGGG TCATAGCTGN TTNNNGTGTG
              901 AANNTGTTAT CCGNTCNCNA NTNNNNCANA ACATANNANN NNGNAAGCAN NAAANTGNAA
              961 ANNCNNGGGG GTGCCTNATG ANTGNANNTA ACNCNCNNNA ATTTGNNNTG NNCNCNNNGN
             1021 CNNNNTTTNC NNNNNGGAAA NNNNNCNNGG NNNNCTNNCA TNNANNNNAT CNGNCNANNN
            1081 NNNNGGGANN NGGNNGGNTT NNNNNNNNTG GGNNNNNNNN NN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl