RDB18294 - CoralHue(TM) dimeric Keima570 (pdKeima570-S1)

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CoralHue(TM) dimeric Keima570 (pdKeima570-S1)

CoralHue™ dimeric Keima570 (pdKeima570-S1)

Catalog number	RDB18294
Resource name	CoralHue(TM) dimeric Keima570 (pdKeima570-S1)
Alternative name	AM-V0121M
Clone info.	・For subcloning. ・Dimeric Keima570 exhibits an extremely large Stokes shift(130 nm) and is stable under usual aerobic conditions. ・Ex/Em(nm): 440/570
Vector backbone	S1 (plasmid)
Size of vector backbone	2.7 kb
Selectable markers	Ampicillin
Gene/insert name	Montipora. sp red fluorescent protein cDNA
Depositor\|Developer	MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., \| Nakajo, Tomohiro \| Karasawa, Satoshi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.

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Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18294	CoralHue(TM) dimeric Keima570 (pdKeima570-S1)	DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full)	RDB18294zzk01.seq provided by the depositor
Remarks, protocol and/or map (png)	RDB18294_map.png

Original reference

original	Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

Further references such as user reports and related articles (go to bottom)

Featured content	Fluorescent protein resources (English text)
Featured content	Fluorescent protein resources (Japanese text)
Featured content	CoralHue™ Fluorescent Protein (Japanese text)
Featured content	CoralHue™ Fluorescent Protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18294_B0H4p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F Region: MCS, insert 5' Sequence file: RDB18294_B0H4a.seq
>D07039F9_B0H4_1_lacIpro-B_F_D01_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGTGAGC GAGGAAGCGG AAGAGCGCCC 61 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG 121 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA 181 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG 241 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGAATTCG AGCTCGGTAC 301 CCGGGGATCC CCACCATGGT GAGTGTGATC GCTAAACAAA TGACCTACAA GGTTTATATG 361 TCAGGCACGG TCAATGGACA CTACTTTGAG GTCGAAGGCG ATGGAAAAGG AAAGCCTTAC 421 GAGGGAGAGC AGACAGTAAA GCTCACTGTC ACCAAGGGTG GACCTCTGCC ATTTGCTTGG 481 GATATATTAT CACCACTGAT GTGTTACGGA AGCATACCAT TCACCAAGTA CCCTGAAGAC 541 ATCCCTGATT ATGTAAAGCA GTCATTCCCT GAGGGATATA CATGGGAGAG GACCATGAAC 601 TTTGAAGATG GTGCAGTGTG TACTGTCAGC AATGATTCCA GCATCCAAGG CAACTGTTTC 661 ATCTACNATG TCAAAATCTC TGGTACGAAC TTTCCTCCCA ATGGACCTGT TATGCAGAAG 721 AAGANACAGG GCTGGGAACC CAGCACTGAN CGTCTCTTTG CACGAGATGN AATGCTGATA 781 GGAAACGATT ATATGGCTCT GAAGTTGGAA NGANGTGGTC ACTATTTGTG TGAGTTCNAA 841 TCTACTTACN ANGCAAAGAA NCCNGTGANG ATGCCNNGGN ATCACTATAT TGANNGCNAA 901 CTGGATGTNN CCNGNCACNA CANGNNNTAC NCATCTGNTN ANCANNNNGA AATANNNNTT 961 GNNNNNNNNN N //
Primer: MBL_S1-rev1 Region: MCS, insert 3' Sequence file: RDB18294_B0H4b.seq
>D07039F9_B0H4_1_MBL_S1-rev1_B11_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGNGCGGGC CTCTTCGCNA TTACGCCNGC 61 TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT 121 CACGACGTTG TAAAACGACG GCCAGTGCCA AGCTTGCATG CCTGCAGTTA ACCGAGCAAA 181 GAGTGGCGTG CAATGGCTAT TTCACACTGC TCAACAGATG TGTAATCCCT GTTGTGACTG 241 GTTACATCCA GTTTGCGGTC AATATAGTGA TACCCTGGCA TCCTCACAGG CTTCTTTGCC 301 TTGTAAGTAG ATTTGAACTC ACACAAATAG TGACCACCTC CTTCCAACTT CAGAGCCATA 361 TAATCGTTTC CTATCAGCAT TCCATCTCGT GCAAAGAGAC GCTCAGTGCT GGGTTCCCAG 421 CCCTGTGTCT TCTTCTGCAT AACAGGTCCA TTGGGAGGAA AGTTCGTACC AGAGATTTTG 481 ACATTGTAGA TGAAACAGTT GCCTTGGATG CTGGAATCAT TGCTGACAGT ACACACTGCA 541 CCATCTTCAA AGTTCATGGT CCTCTCCCAT GTATATCCCT CAGGGAATGA CTGCTTTACA 601 TNNNCNGGGA TGTCTTCAGG GTACTTGGTG AATGGTATGC TTCCGTAACA CATCAGTGGT 661 GATAATATAT CCCAAGCAAA TGGCAGANGT CCACCCTTGG TGACAGTGAG CTTTACTGTC 721 TGCTCTCCCT CGTAAGGCTT TCCTTTTCCA TCGCCTTCGA CCTCNAAAGT ANTGTCCATT 781 GNNCGTGCCT GACATATAAA CCNTNNNNGT CATTTGTTTA NCGATCACAC TCACCATGGT 841 GGGGATCCCC GGNTANCGAN CTCNAATTCN TAATCATGGG TCATAGCTGN TTNNNGTGTG 901 AANNTGTTAT CCGNTCNCNA NTNNNNCANA ACATANNANN NNGNAAGCAN NAAANTGNAA 961 ANNCNNGGGG GTGCCTNATG ANTGNANNTA ACNCNCNNNA ATTTGNNNTG NNCNCNNNGN 1021 CNNNNTTTNC NNNNNGGAAA NNNNNCNNGG NNNNCTNNCA TNNANNNNAT CNGNCNANNN 1081 NNNNGGGANN NGGNNGGNTT NNNNNNNNTG GGNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

2021.08.13

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