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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) dimeric Keima570 (pdKeima570-S1) (#RDB18294)

CoralHue™ dimeric Keima570 (pdKeima570-S1)

Alternative name AM-V0121M
Clone info. ・For subcloning.
・Dimeric Keima570 exhibits an extremely large Stokes shift(130 nm) and is stable under usual aerobic conditions.
・Ex/Em(nm): 440/570
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18294zzk01.seq provided by the depositor
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Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

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RDB18294 CoralHue(TM) dimeric Keima570 (pdKeima570-S1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) dimeric Keima570 (pdKeima570-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18294).

Reference section:

Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M., Miyawaki, A., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18294_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: MCS, insert 5'
Sequence file: RDB18294_B0H4a.seq check
>D07039F9_B0H4_1_lacIpro-B_F_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGTGAGC GAGGAAGCGG AAGAGCGCCC
   61 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG
  121 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA
  181 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG
  241 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGAATTCG AGCTCGGTAC
  301 CCGGGGATCC CCACCATGGT GAGTGTGATC GCTAAACAAA TGACCTACAA GGTTTATATG
  361 TCAGGCACGG TCAATGGACA CTACTTTGAG GTCGAAGGCG ATGGAAAAGG AAAGCCTTAC
  421 GAGGGAGAGC AGACAGTAAA GCTCACTGTC ACCAAGGGTG GACCTCTGCC ATTTGCTTGG
  481 GATATATTAT CACCACTGAT GTGTTACGGA AGCATACCAT TCACCAAGTA CCCTGAAGAC
  541 ATCCCTGATT ATGTAAAGCA GTCATTCCCT GAGGGATATA CATGGGAGAG GACCATGAAC
  601 TTTGAAGATG GTGCAGTGTG TACTGTCAGC AATGATTCCA GCATCCAAGG CAACTGTTTC
  661 ATCTACNATG TCAAAATCTC TGGTACGAAC TTTCCTCCCA ATGGACCTGT TATGCAGAAG
  721 AAGANACAGG GCTGGGAACC CAGCACTGAN CGTCTCTTTG CACGAGATGN AATGCTGATA
  781 GGAAACGATT ATATGGCTCT GAAGTTGGAA NGANGTGGTC ACTATTTGTG TGAGTTCNAA
  841 TCTACTTACN ANGCAAAGAA NCCNGTGANG ATGCCNNGGN ATCACTATAT TGANNGCNAA
  901 CTGGATGTNN CCNGNCACNA CANGNNNTAC NCATCTGNTN ANCANNNNGA AATANNNNTT
961 GNNNNNNNNN N
//
Primer: MBL_S1-rev1 (Pr0941)
Region: MCS, insert 3'
Sequence file: RDB18294_B0H4b.seq check
>D07039F9_B0H4_1_MBL_S1-rev1_B11_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGNGCGGGC CTCTTCGCNA TTACGCCNGC
   61 TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT
  121 CACGACGTTG TAAAACGACG GCCAGTGCCA AGCTTGCATG CCTGCAGTTA ACCGAGCAAA
  181 GAGTGGCGTG CAATGGCTAT TTCACACTGC TCAACAGATG TGTAATCCCT GTTGTGACTG
  241 GTTACATCCA GTTTGCGGTC AATATAGTGA TACCCTGGCA TCCTCACAGG CTTCTTTGCC
  301 TTGTAAGTAG ATTTGAACTC ACACAAATAG TGACCACCTC CTTCCAACTT CAGAGCCATA
  361 TAATCGTTTC CTATCAGCAT TCCATCTCGT GCAAAGAGAC GCTCAGTGCT GGGTTCCCAG
  421 CCCTGTGTCT TCTTCTGCAT AACAGGTCCA TTGGGAGGAA AGTTCGTACC AGAGATTTTG
  481 ACATTGTAGA TGAAACAGTT GCCTTGGATG CTGGAATCAT TGCTGACAGT ACACACTGCA
  541 CCATCTTCAA AGTTCATGGT CCTCTCCCAT GTATATCCCT CAGGGAATGA CTGCTTTACA
  601 TNNNCNGGGA TGTCTTCAGG GTACTTGGTG AATGGTATGC TTCCGTAACA CATCAGTGGT
  661 GATAATATAT CCCAAGCAAA TGGCAGANGT CCACCCTTGG TGACAGTGAG CTTTACTGTC
  721 TGCTCTCCCT CGTAAGGCTT TCCTTTTCCA TCGCCTTCGA CCTCNAAAGT ANTGTCCATT
  781 GNNCGTGCCT GACATATAAA CCNTNNNNGT CATTTGTTTA NCGATCACAC TCACCATGGT
  841 GGGGATCCCC GGNTANCGAN CTCNAATTCN TAATCATGGG TCATAGCTGN TTNNNGTGTG
  901 AANNTGTTAT CCGNTCNCNA NTNNNNCANA ACATANNANN NNGNAAGCAN NAAANTGNAA
  961 ANNCNNGGGG GTGCCTNATG ANTGNANNTA ACNCNCNNNA ATTTGNNNTG NNCNCNNNGN
 1021 CNNNNTTTNC NNNNNGGAAA NNNNNCNNGG NNNNCTNNCA TNNANNNNAT CNGNCNANNN
1081 NNNNGGGANN NGGNNGGNTT NNNNNNNNTG GGNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]