CoralHue™ humanized monomeric Azami-Green 1 (phmAG1-MC1)
Alternative name | AM-V0035M |
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Clone info. | ・Fluorescent protein-MCS/For mammalian expression of the fluorescent protein or its fusion. ・The codons are humanized. ・Monomeric Azami-Green1 is green fluorescent protein that is stable in acidic and basic conditions without significant loss of signal. ・Ex/Em(nm): 492/505 |
Vector backbone | MC1 (plasmid) |
Size of vector backbone | 3.9 kb |
Selectable markers | Kanamycin (E. coli), Neomycin (mammalian cells) |
Gene/insert name | Galaxea fascicularis green fluorescent protein cDNA |
Depositor|Developer | MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi | |
Sequence (full) | RDB18250zzk02.seq provided by the depositor | Additional map |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18250 | CoralHue(TM) humanized monomeric Azami-Green 1 (phmAG1-MC1) | DNA solution |
Materials & Methods section:
The CoralHue(TM) humanized monomeric Azami-Green 1 (phmAG1-MC1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18250). |
Reference section:
Karasawa, S., Araki, T., Yamamoto-Hino, M., Miyawaki, A., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18250_B0H4p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5' Sequence file: RDB18250_B0H4a.seq |
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>D07039B1_B0H4_2_CMV_Forward_A07_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NGNNNNNGGG CGGTNNGCGN GTACGGNGGG NNGTCTATAT 61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCAT TGCCACCATG GTGAGCGTGA 121 TCAAGCCCGA GATGAAGATC AAGCTGTGCA TGAGGGGCAC CGTGAACGGC CACAACTTCG 181 TGATCGAGGG CGAGGGCAAG GGCAACCCCT ACGAGGGCAC CCAGATCCTG GACCTGAACG 241 TGACCGAGGG CGCCCCCCTG CCCTTCGCCT ACGACATCCT GACCACCGTG TTCCAGTACG 301 GCAACAGGGC CTTCACCAAG TACCCCGCCG ACATCCAGGA CTACTTCAAG CAGACCTTCC 361 CCGAGGGCTA CCACTGGGAG AGGAGCATGA CCTACGAGGA CCAGGGCATC TGCACCGCCA 421 CCAGCAACAT CAGCATGAGG GGCGACTGCT TCTTCTACGA CATCAGGTTC GACGGCACCA 481 ACTTCCCCCC CAACGGCCCC GTGATGCAGA AGAAGACCCT GAAGTGGGAG CCCAGCACCG 541 AGAAGATGTA CGTGGAGGAC GGCGTGCTGA AGGGCGACGT GAACATGAGG CTGCTGCTGG 601 AGGGCGGCGG CCACTACAGG TGCGACTTCA AGACCACCTA CAAGGCCAAG AANGAGGTGA 661 GGCTGCCCGA CGCCCACAAG ATCGACCACA GGATCGAGAT CCTGAAGCAC GACAAGGACT 721 ACAACAAGGT GAAGCTGTAC GANAACGCCG TGGCCAGGTA CTCCATGCTG CCCAGCCAGG 781 CCAANGGNTC CTCANGTACC GGAACTGCAN CAGANAATTC GGGAAACTCN NNAACAAAGC 841 TTGAATAANC GGCCGCNANT CTANATCATA ATCAGCCATA NNACATTTGN AGAGGTTTTA 901 CTNGCNTTAA AAAANNNNNN NCANCNNCCC CNNNNNNCNN NNNNNNNN // |
Primer: SV40polyA_R2 (Pr0623) Region: MCS, insert 3' Sequence file: RDB18250_B0H4b.seq |
>D07039B1_B0H4_2_SV40polyA-R2_A08_02_ABI24.ab1 1 NNNNNNNNNN NCNNNANNNN NNGTTNANNA CNACAATTGC ATTCNTTTTA TGTTTCAGGT 61 TCAGGGGGAG GTGTGGGANG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC 121 TGATTATGAT CTAGAGTCGC GGCCGCTTAT TCAAGCTTTG TTCTCGAGTT TCCCGAATTC 181 TCTGCTGCAG TTCCGGTACC TGAGGATCCC TTGGCCTGGC TGGGCAGCAT GGAGTACCTG 241 GCCACGGCGT TCTCGTACAG CTTCACCTTG TTGTAGTCCT TGTCGTGCTT CAGGATCTCG 301 ATCCTGTGGT CGATCTTGTG GGCGTCGGGC AGCCTCACCT CCTTCTTGGC CTTGTAGGTG 361 GTCTTGAAGT CGCACCTGTA GTGGCCGCCG CCCTCCAGCA GCAGCCTCAT GTTCACGTCG 421 CCCTTCAGCA CGCCGTCCTC CACGTACATC TTCTCGGTGC TGGGCTCCCA CTTCAGGGTC 481 TTCTTCTGCA TCACGGGGCC GTTGGGGGGG AAGTTGGTGC CGTCGAACCT GATGTCGTAG 541 AAGAAGCAGT CGCCCCTCAT GCTGATGTTG CTGGTGGCGG TGCAGATGCC CTGGTCCTCG 601 TAGGTCATGC TCCTCTCCCA GTGGTAGCCC TCGGGGAANG TCTGCTTGAA GTAGTCCTGG 661 ATGTCGGCGG GGTACTTGGT GAANGCCCTG TTGCCGTACT GGAACACGGT GGTCAGGATG 721 TCGTANGCGA ANGGCANGGG GGCGCCCTCN GTCACGTTCA NGTCCAGGAT CTGNNNGCCC 781 TCNTANGGGT TGCCNNTGCC CTCGCCCTCN NTCACNAANT TGTGGCCGNT CACGGNGCCC 841 CTCANGCACA GCTNNNTCTN CATCTCNGGN TTNNTCACGC NCACCNNGGN NNNNANNCTA 901 NCGNNNNNNN NNN // |
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB18250_B0H4c.seq |
>D07039B1_B0H4_2_SV40pro_ori_F_G10_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NCCTCNNNCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT 61 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC ATCAAGAGAC AGGATGAGGA TCGTTTCGCA 121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG 181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG 241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC 301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC 361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG 421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC 481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA 541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG 601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG 661 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG 721 GCCGCTTTTC TGGATTCATC GACTGTGGCN GGCTGGGTGT GGCGGANCGC TATCAGGANA 781 TAGCGTTGGC TACCCGNGAT ATTGCTGAAN ANCTTGGCGG CGAATGGGCT GANCGCTTCC 841 TCGTGCTTTA CNGNATCGCC GCTCCCGANN CNCANCGCAT CNCCTTCTAT CGCCTTCTTG 901 ANNANNTNNT TCTGANCGGG NNTCNNGGGG TTCNAAANGA NCNNACNNAN CGACNNCNNA 961 NNCNNNNCNN NNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP] |
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