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CoralHue(TM) humanized monomeric Azami-Green 1 (phmAG1-MC1)

CoralHue™ humanized monomeric Azami-Green 1 (phmAG1-MC1)

Catalog number RDB18250
Resource name CoralHue(TM) humanized monomeric Azami-Green 1 (phmAG1-MC1)
Alternative name AM-V0035M
Clone info. ・Fluorescent protein-MCS/For mammalian expression of the fluorescent protein or its fusion.
・The codons are humanized.
・Monomeric Azami-Green1 is green fluorescent protein that is stable in acidic and basic conditions without significant loss of signal.
・Ex/Em(nm): 492/505
Vector backbone MC1 (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Galaxea fascicularis green fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18250 CoralHue(TM) humanized monomeric Azami-Green 1 (phmAG1-MC1) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB18250zzk02.seq
Electronic file Map RDB18250_map.png

Original reference

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content CoralHue™ Fluorescent Protein (Japanese text)
Featured content CoralHue™ Fluorescent Protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18250_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB18250_B0H4a.seq check
>D07039B1_B0H4_2_CMV_Forward_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNNNNGGG CGGTNNGCGN GTACGGNGGG NNGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCAT TGCCACCATG GTGAGCGTGA
  121 TCAAGCCCGA GATGAAGATC AAGCTGTGCA TGAGGGGCAC CGTGAACGGC CACAACTTCG
  181 TGATCGAGGG CGAGGGCAAG GGCAACCCCT ACGAGGGCAC CCAGATCCTG GACCTGAACG
  241 TGACCGAGGG CGCCCCCCTG CCCTTCGCCT ACGACATCCT GACCACCGTG TTCCAGTACG
  301 GCAACAGGGC CTTCACCAAG TACCCCGCCG ACATCCAGGA CTACTTCAAG CAGACCTTCC
  361 CCGAGGGCTA CCACTGGGAG AGGAGCATGA CCTACGAGGA CCAGGGCATC TGCACCGCCA
  421 CCAGCAACAT CAGCATGAGG GGCGACTGCT TCTTCTACGA CATCAGGTTC GACGGCACCA
  481 ACTTCCCCCC CAACGGCCCC GTGATGCAGA AGAAGACCCT GAAGTGGGAG CCCAGCACCG
  541 AGAAGATGTA CGTGGAGGAC GGCGTGCTGA AGGGCGACGT GAACATGAGG CTGCTGCTGG
  601 AGGGCGGCGG CCACTACAGG TGCGACTTCA AGACCACCTA CAAGGCCAAG AANGAGGTGA
  661 GGCTGCCCGA CGCCCACAAG ATCGACCACA GGATCGAGAT CCTGAAGCAC GACAAGGACT
  721 ACAACAAGGT GAAGCTGTAC GANAACGCCG TGGCCAGGTA CTCCATGCTG CCCAGCCAGG
  781 CCAANGGNTC CTCANGTACC GGAACTGCAN CAGANAATTC GGGAAACTCN NNAACAAAGC
  841 TTGAATAANC GGCCGCNANT CTANATCATA ATCAGCCATA NNACATTTGN AGAGGTTTTA
901 CTNGCNTTAA AAAANNNNNN NCANCNNCCC CNNNNNNCNN NNNNNNNN
//
Primer: SV40polyA_R2
Region: MCS, insert 3'
Sequence file: RDB18250_B0H4b.seq check
>D07039B1_B0H4_2_SV40polyA-R2_A08_02_ABI24.ab1
    1 NNNNNNNNNN NCNNNANNNN NNGTTNANNA CNACAATTGC ATTCNTTTTA TGTTTCAGGT
   61 TCAGGGGGAG GTGTGGGANG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
  121 TGATTATGAT CTAGAGTCGC GGCCGCTTAT TCAAGCTTTG TTCTCGAGTT TCCCGAATTC
  181 TCTGCTGCAG TTCCGGTACC TGAGGATCCC TTGGCCTGGC TGGGCAGCAT GGAGTACCTG
  241 GCCACGGCGT TCTCGTACAG CTTCACCTTG TTGTAGTCCT TGTCGTGCTT CAGGATCTCG
  301 ATCCTGTGGT CGATCTTGTG GGCGTCGGGC AGCCTCACCT CCTTCTTGGC CTTGTAGGTG
  361 GTCTTGAAGT CGCACCTGTA GTGGCCGCCG CCCTCCAGCA GCAGCCTCAT GTTCACGTCG
  421 CCCTTCAGCA CGCCGTCCTC CACGTACATC TTCTCGGTGC TGGGCTCCCA CTTCAGGGTC
  481 TTCTTCTGCA TCACGGGGCC GTTGGGGGGG AAGTTGGTGC CGTCGAACCT GATGTCGTAG
  541 AAGAAGCAGT CGCCCCTCAT GCTGATGTTG CTGGTGGCGG TGCAGATGCC CTGGTCCTCG
  601 TAGGTCATGC TCCTCTCCCA GTGGTAGCCC TCGGGGAANG TCTGCTTGAA GTAGTCCTGG
  661 ATGTCGGCGG GGTACTTGGT GAANGCCCTG TTGCCGTACT GGAACACGGT GGTCAGGATG
  721 TCGTANGCGA ANGGCANGGG GGCGCCCTCN GTCACGTTCA NGTCCAGGAT CTGNNNGCCC
  781 TCNTANGGGT TGCCNNTGCC CTCGCCCTCN NTCACNAANT TGTGGCCGNT CACGGNGCCC
  841 CTCANGCACA GCTNNNTCTN CATCTCNGGN TTNNTCACGC NCACCNNGGN NNNNANNCTA
901 NCGNNNNNNN NNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB18250_B0H4c.seq check
>D07039B1_B0H4_2_SV40pro_ori_F_G10_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCCTCNNNCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
  121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
  181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG
  241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC
  301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC
  361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
  421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
  481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA
  541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG
  601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG
  661 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
  721 GCCGCTTTTC TGGATTCATC GACTGTGGCN GGCTGGGTGT GGCGGANCGC TATCAGGANA
  781 TAGCGTTGGC TACCCGNGAT ATTGCTGAAN ANCTTGGCGG CGAATGGGCT GANCGCTTCC
  841 TCGTGCTTTA CNGNATCGCC GCTCCCGANN CNCANCGCAT CNCCTTCTAT CGCCTTCTTG
  901 ANNANNTNNT TCTGANCGGG NNTCNNGGGG TTCNAAANGA NCNNACNNAN CGACNNCNNA
961 NNCNNNNCNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

2021.01.15

GNP_filter3_RDBDEP_html_201222.pl