RDB18233 - pCI-neo-bPCFT

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pCI-neo-bPCFT (#RDB18233)

Expression vector of bovine PCFT.

Clone info.	Expression vector of bovine proton-coupled folate transporter/heme carrier protein 1 (PCFT).
Comment	A reference nucleotide sequence for Bovine PCFT is NM_001079585.1.
Vector backbone	pCl-neo (plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name	cattle SLC46A1 cDNA
Depositor\|Developer	Yuasa, Hiroaki \|

Sequence (full)	RDB18233zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。当該利用者によるリソースの利用が第三者の産業財産権をはじめとする一切の知的財産権を侵害しない旨の保証をせず、かつ商品性又は特定目的への適合性の保証をしない。また、当該利用者による本件成果有体物の使用、保有によって発生した如何なる結果、損害についても一切その責任を負担せず、かつ、如何なる損害賠償義務（直接、間接損害を問わない。）も負わない。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。当該利用者によるリソースの利用が第三者の産業財産権をはじめとする一切の知的財産権を侵害しない旨の保証をせず、かつ商品性又は特定目的への適合性の保証をしない。また、当該利用者による本件成果有体物の使用、保有によって発生した如何なる結果、損害についても一切その責任を負担せず、かつ、如何なる損害賠償義務（直接、間接損害を問わない。）も負わない。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18233	pCI-neo-bPCFT	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCI-neo-bPCFT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18233).

Reference section:

Yamashiro, T., Yasujima, T., Ohta, K., Inoue, K., Yuasa, H., Identification of the amino acid residue responsible for the myricetin sensitivity of human proton-coupled folate transporter. Sci. Rep. 9 (1): 18105 (2019). PMID 31792273. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18233_B0Fup1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: chimeric intron 3-prime (Pr0444) Region: T7 pro, insert 5' Sequence file: RDB18233_B0Fua.seq
>D07213A4_B0Fu_1_chimeric intron3-prime_D05_11_ABI24.ab1 1 NNNNNNNNCN NNNNNNTNAC NGCTCTTAAG GCTAGAGTAC TTAATACGAC TCACTATAGG 61 CTAGCCTCGA GAGCATGGAG GGACGCGCGA ACTCCCCGGG CGAGCCCCGC GCCTGGCCTA 121 CCCGGTCCGT GCTGTGCCGC GGCTGCGTGG AGCCACTCGT CTTCCTCGCC AACTTCGCCT 181 TGGTCCTGCA GGGCCCGGTC ACCACGCAGT ACCTGTGGCA CCGTTTCAGT GCAGACCTTG 241 GCTACAATGG CACCCGTCAC AGGGACAGCT GCAGCAACCA CAGCGTGGAC CCCATCGCGC 301 AGGAAGTGGA GACACTTACC TCCCATTGGA CCCTCTACAT GAACGTGGGC GGCTTCCTGG 361 TGGGACTCTT CTCGTCCACC CTGCTGGGCG CCTGGAGTGA CTGTGTGGGC CGCCGCCCGC 421 TGCTGGTGCT GGCCTCCCTC GGCCTGCTGC TCCAGACCGT GCTGTCCATC TTCGTGGTTC 481 AGCTGCATCT CCACATTGGC TACTTGGTAC TGGGCCGCAT CCTTTGTGCC CTCCTCGGCG 541 ATTTCAGTGG GCTCCTGGCC GCCAGCTTTG CCTCCGTGGC TGATGTCAGC TCCAGCCGCA 601 CCCGTACCAT CCGAATGGCC CTGTTGGAAN CATGCATTGG GGTGGCGGGA ATGCTGGCGA 661 GCTTCATTGG TGGCTTCTTG CTCCNGGANC ANGTTTATGT CAACCCCTTC TGGCTCGCCT 721 TGGCCGNGCT GACCGTCATG ANTCTCTATG CAGCATTCTG CTTTGGTGAN ANAGNGAANG 781 ANCNGANACC TACCNNNCTC TTCACACTCC NNCACCACCN GNCCGNCNTC CANCTCNATG 841 NNANCNANGC CCNNNNNNNN NCNNNGAANC NCTNNNCCNN NNNNNCNNNA NNNNNNNNNN //
Primer: pAxCALNLF1 (Pr0166) Region: T3 pro, insert 3' Sequence file: RDB18233_B0Fub.seq
>D07213A4_B0Fu_1_pAxCALNLF1_E05_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNCTGCTCNN NNCNNTAACC CTCACTAAAG GGAAGCGGCC 61 GCTCAGGGGC TCTGGGAAAA CTCCTGGAAT TCAGGGCAAT GATTATCCCT TTCCAGTATC 121 CCCATCAGGA TGGCTGGGAT GAATAGCAGT CCAGCTGCCA GGAGGAAAGG GAAGCCCTTC 181 ATGAGGTTCA GGGTGGCTGG GTAGAGCGAG TTGAAGATGC CGGAGGCCAT CAGCATGGCC 241 AAGCCATTCA CACAGGCCAG AGCAGAAAAG AGAGCGCCCT GCTCCGATTG CCTCACCAGT 301 CTAGATAGCT TGGCCCGGAT GATAGGGGTG ACGACCAGTG ACAGGAAAAG GAGCCCGTAC 361 CCTGTGAACA TGAGGGGTGT GATGGTAGCA AATGCAAAGA CCATCATCCC CAGGATATTG 421 AAGACCAGGC CGATCTCAGC CACCCAGGTG TCGGCCAGGC AGTACTGCAG AAGCCGCAGG 481 CCCAGCAGGC TGGTGAGGTA TGGGAGCTGC TGAGCTGCAG AACCGTAGCT GATCAGCCGA 541 GAGTCCCAGC AGAGGGGTGC ACTCAGCTCA TAGAGGGTCA GGATGTCCTG GGCCCCGAGG 601 TGCACAGTGA TCATCACGAA GATGGCTAAT GAATACAGGG CTAAGTGCTT CCTGGACTTC 661 TCCGGGGCCT GGGTCACATA NAGCTGGATG ACGGACCGGT GGTGACGGAN TGTGAAGAGA 721 CNGGTAGGTG TCCGCTCCTT CACTGTCTCA CCAAAGCAGA NTGCTGCATA GANNGTCATG 781 ACGGTCAGCA CGGNCAANGC NANCCAGAAN GGGTTGACAT ANNCCNGCTC CTGGNNCAAG 841 AANCCNCCNA TGANNCTCGC CAGCATTCCC GCNNNCNNNN ANGNATGCNT NCAANANGGN 901 NCATTCNGNN GGNANNGNNN NNGNNGGNNC TNNNNNNNNN N //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, NeoR_KanR Sequence file: RDB18233_B0Fuc.seq
>D07213A4_B0Fu_1_SV40pro_ori_F_F05_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNCC TCNGCCTCTN NNCTATTCNN GAANTAGTGA GGAGGCTTTT 61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTTG ATTCTTCTGA CACAACAGTC TCGAACTTAA 121 GGCTAGAGCC ACCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT 181 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT 241 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC 301 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC 361 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA 421 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT 481 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA 541 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA 601 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC 661 GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT 721 CATGGTGGAA AATGGCCGCT TTTCTGGANT CATCGNCTGT GGCCGGCTGG GNGTGNCGGA 781 NCGCTATCAN GANATAGCGT TGGCTACCCG NGANATTGCT GAANANCTTG GNGGCGAANG 841 GGCNNNNCNC TTCCNCNNNC TTTANNGNAT CGCCNCNCCN NANTCNCANC GCNNCNNNTN 901 CNATCNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yamashiro, T., Identification of the amino acid residue responsible for the myricetin sensitivity of human proton-coupled folate transporter. Sci. Rep. 9 (1): 18105 (2019). PMID 31792273. [link to RRC of NBRP]

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Other resources in our bank

cattle SLC46A1 and ortholog

External Database
cattle SLC46A1

Reference sequence