Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCI-neo-bPCFT

Expression vector of bovine PCFT.

Catalog number RDB18233
Resource name pCI-neo-bPCFT
Clone info. Expression vector of bovine proton-coupled folate transporter/heme carrier protein 1 (PCFT).
Comment A reference nucleotide sequence for Bovine PCFT is NM_001079585.1.
Vector backbone pCl-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name cattle SLC46A1 cDNA
Depositor|Developer Yuasa, Hiroaki |
Other clones in our bank

External Database
cattle SLC46A1

          Reference sequence
            
           
          Sequence (full) RDB18233zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。当該利用者によるリソースの利用が第三者の産業財産権をはじめとする一切の知的財産権を侵害しない旨の保証をせず、かつ商品性又は特定目的への適合性の保証をしない。また、当該利用者による本件成果有体物の使用、保有によって発生した如何なる結果、損害についても一切その責任を負担せず、かつ、如何なる損害賠償義務(直接、間接損害を問わない。)も負わない。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18233 pCI-neo-bPCFT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Yamashiro, T., Identification of the amino acid residue responsible for the myricetin sensitivity of human proton-coupled folate transporter. Sci. Rep. 9 (1): 18105 (2019). PMID 31792273.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18233_B0Fup1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: chimeric intron 3-prime
          Region: T7 pro, insert 5'
          Sequence file: RDB18233_B0Fua.seq check
          >D07213A4_B0Fu_1_chimeric intron3-prime_D05_11_ABI24.ab1
              1 NNNNNNNNCN NNNNNNTNAC NGCTCTTAAG GCTAGAGTAC TTAATACGAC TCACTATAGG
             61 CTAGCCTCGA GAGCATGGAG GGACGCGCGA ACTCCCCGGG CGAGCCCCGC GCCTGGCCTA
            121 CCCGGTCCGT GCTGTGCCGC GGCTGCGTGG AGCCACTCGT CTTCCTCGCC AACTTCGCCT
            181 TGGTCCTGCA GGGCCCGGTC ACCACGCAGT ACCTGTGGCA CCGTTTCAGT GCAGACCTTG
            241 GCTACAATGG CACCCGTCAC AGGGACAGCT GCAGCAACCA CAGCGTGGAC CCCATCGCGC
            301 AGGAAGTGGA GACACTTACC TCCCATTGGA CCCTCTACAT GAACGTGGGC GGCTTCCTGG
            361 TGGGACTCTT CTCGTCCACC CTGCTGGGCG CCTGGAGTGA CTGTGTGGGC CGCCGCCCGC
            421 TGCTGGTGCT GGCCTCCCTC GGCCTGCTGC TCCAGACCGT GCTGTCCATC TTCGTGGTTC
            481 AGCTGCATCT CCACATTGGC TACTTGGTAC TGGGCCGCAT CCTTTGTGCC CTCCTCGGCG
            541 ATTTCAGTGG GCTCCTGGCC GCCAGCTTTG CCTCCGTGGC TGATGTCAGC TCCAGCCGCA
            601 CCCGTACCAT CCGAATGGCC CTGTTGGAAN CATGCATTGG GGTGGCGGGA ATGCTGGCGA
            661 GCTTCATTGG TGGCTTCTTG CTCCNGGANC ANGTTTATGT CAACCCCTTC TGGCTCGCCT
            721 TGGCCGNGCT GACCGTCATG ANTCTCTATG CAGCATTCTG CTTTGGTGAN ANAGNGAANG
            781 ANCNGANACC TACCNNNCTC TTCACACTCC NNCACCACCN GNCCGNCNTC CANCTCNATG
            841 NNANCNANGC CCNNNNNNNN NCNNNGAANC NCTNNNCCNN NNNNNCNNNA NNNNNNNNNN
          //
          Primer: pAxCALNLF1
          Region: T3 pro, insert 3'
          Sequence file: RDB18233_B0Fub.seq check
          >D07213A4_B0Fu_1_pAxCALNLF1_E05_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNCTGCTCNN NNCNNTAACC CTCACTAAAG GGAAGCGGCC
             61 GCTCAGGGGC TCTGGGAAAA CTCCTGGAAT TCAGGGCAAT GATTATCCCT TTCCAGTATC
            121 CCCATCAGGA TGGCTGGGAT GAATAGCAGT CCAGCTGCCA GGAGGAAAGG GAAGCCCTTC
            181 ATGAGGTTCA GGGTGGCTGG GTAGAGCGAG TTGAAGATGC CGGAGGCCAT CAGCATGGCC
            241 AAGCCATTCA CACAGGCCAG AGCAGAAAAG AGAGCGCCCT GCTCCGATTG CCTCACCAGT
            301 CTAGATAGCT TGGCCCGGAT GATAGGGGTG ACGACCAGTG ACAGGAAAAG GAGCCCGTAC
            361 CCTGTGAACA TGAGGGGTGT GATGGTAGCA AATGCAAAGA CCATCATCCC CAGGATATTG
            421 AAGACCAGGC CGATCTCAGC CACCCAGGTG TCGGCCAGGC AGTACTGCAG AAGCCGCAGG
            481 CCCAGCAGGC TGGTGAGGTA TGGGAGCTGC TGAGCTGCAG AACCGTAGCT GATCAGCCGA
            541 GAGTCCCAGC AGAGGGGTGC ACTCAGCTCA TAGAGGGTCA GGATGTCCTG GGCCCCGAGG
            601 TGCACAGTGA TCATCACGAA GATGGCTAAT GAATACAGGG CTAAGTGCTT CCTGGACTTC
            661 TCCGGGGCCT GGGTCACATA NAGCTGGATG ACGGACCGGT GGTGACGGAN TGTGAAGAGA
            721 CNGGTAGGTG TCCGCTCCTT CACTGTCTCA CCAAAGCAGA NTGCTGCATA GANNGTCATG
            781 ACGGTCAGCA CGGNCAANGC NANCCAGAAN GGGTTGACAT ANNCCNGCTC CTGGNNCAAG
            841 AANCCNCCNA TGANNCTCGC CAGCATTCCC GCNNNCNNNN ANGNATGCNT NCAANANGGN
          901 NCATTCNGNN GGNANNGNNN NNGNNGGNNC TNNNNNNNNN N
          //
          Primer: SV40pro_ori_F
          Region: SV40pro_ori, NeoR_KanR
          Sequence file: RDB18233_B0Fuc.seq check
          >D07213A4_B0Fu_1_SV40pro_ori_F_F05_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNCC TCNGCCTCTN NNCTATTCNN GAANTAGTGA GGAGGCTTTT
             61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTTG ATTCTTCTGA CACAACAGTC TCGAACTTAA
            121 GGCTAGAGCC ACCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
            181 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
            241 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
            301 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
            361 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
            421 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
            481 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
            541 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
            601 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC
            661 GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
            721 CATGGTGGAA AATGGCCGCT TTTCTGGANT CATCGNCTGT GGCCGGCTGG GNGTGNCGGA
            781 NCGCTATCAN GANATAGCGT TGGCTACCCG NGANATTGCT GAANANCTTG GNGGCGAANG
            841 GGCNNNNCNC TTCCNCNNNC TTTANNGNAT CGCCNCNCCN NANTCNCANC GCNNCNNNTN
          901 CNATCNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yamashiro, T., Identification of the amino acid residue responsible for the myricetin sensitivity of human proton-coupled folate transporter. Sci. Rep. 9 (1): 18105 (2019). PMID 31792273.

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl