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Human OSTC promoter P1

Luciferase reporter vector of human OSTC P1 promoter -301 to +19.

Catalog number RDB18166
Resource name Human OSTC promoter P1
Clone info. Luciferase reporter vector of human oligosaccharyltransferase complex non-catalytic subunit OSTC P1 promoter -301 to +19.
Comment Expression was confirmed by the depositor with luciferase assay.
PCR cloning: forward primer (5'- ATA ACG CGT TTC AAA TCC CCT GCG TCA GC -3'), reverse primer (5'- ATA AGA TCT AAC AGG CCC GCC CTC CCT -3'). The positions of the transcription start sites were determined using UCSC Genome Browser GRCh38/hg38.
Vector backbone pGL3-basic (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name human OSTC genomic
Depositor|Developer Unoki, Motoko |
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External Database
human OSTC
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18166 Human OSTC promoter P1 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Sequence (partial) RDB18166pka01.seq provided by the depositor

          Original reference

          original Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277.

          2022.05.16

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