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pEGFP-C1-Rab45 (#RDB17958)

Expression vector of mouse Rab45) tagged with EGFP.

Clone info. Expression vector of mouse RAS and EF hand domain containing (Rab45) tagged with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells).
Gene/insert name mouse Rasef cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17958 pEGFP-C1-Rab45 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab45 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17958).

Reference section:

Marubashi, S., Fukuda, M., Rab7B/42 Is Functionally Involved in Protein Degradation on Melanosomes in Keratinocytes. Cell Struct. Funct. 45 (1): 45-55 (2020). PMID 32037382. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17958_B0Cbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5'
Sequence file: RDB17958_B0Cba.seq check
>D07009A8_B0Cb_3_EGFP-C2_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNC NCNTGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGNTCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGCTCAAG AGGTGTCTTC
  121 CAGTGTGGCT TTCATCCATA GACTGCTATA TACAGCTCTA CGTGGCCTGC TTGGAGACAG
  181 ACTGAAACGA GGCTTGAAGA AAGAACAAGT TAGTACCCTG TACCAGAACA TCACCCTTGT
  241 GGAGCCAAGG CTACTCCAAC CGTATGAACG TGTGATAAGG AACTTTCTCC GTGAGATCAA
  301 ACTCCAGAGC ACAGAGATGG AAAACCTGGC CATTGCAGTG AAGAGAGCCC AGGACAAGGC
  361 GGCCATTCAG CTAAGTGAGC TGGAAGAGGA AATGGATCAG AGGATTCAAG CTGTGGAAAA
  421 TGAGTCCCGG AAAGATGAAA AGCGCAAAGC TGAGGAGGCT CTCACTGACC TCAGGCGTCA
  481 GTATGAAACA GAAGTAGGAG ACTTACAAGT GACCATTAAA AGGTTAAAAA AGCTTGAAGA
  541 GCAATCAAGA CAAATAAGTC AAAAGCAAGA TGTGACGGCA TTGAAGAAGC AAATCCATGA
  601 TTTAACAATG GAAAATCAAA AACTCAAGAA AGAACTTTTG GAAGCACAGA CAAACGTAGC
  661 CTTCCTTCAG AGCGAACTAG ATGCTTTGAA AAGTGATTAT GCTGACCAGA GTCTGAATTC
  721 TGAACGGGAT CTGGAAATAA TCCGAGAATA CACAGAAGAT CGAAGCAGTC TTGAGAGGCA
  781 AATTGAAATC CTCCAAACAG CTAACCGGAA GCTGCATGAC AGTAATGATG GCCTCANGAN
  841 TGCACNGGAA AACACTTACA GCAAGCTCNA CAGATCCTNG CGTATNAATA ACATATCTNN
  901 NGGGAATNCN ATTTCNAGAA NCAGTCCCAA ATTNNATCNT CANTCCNNNC ANCCNCNGNN
961 CNATNNNNNN NCNNNNNNTN NNNCANNNGN NGNNNNNNAN NNNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17958_B0Cbb.seq check
>D07009A8_B0Cb_3_SV40polyA_R2_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNGNNNNNNN NNANNNNNNN NNNNANNATT GCNTTCNTTT TATGTTTCNG
   61 GTTCNGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
  121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACCTG
  181 CAGGCGGCCG CGAATTCACT AGTGATTTTA GCCATTGCAA CAGTTCTTCA TCTGCAGTGA
  241 CTTTTTAGAA GTAGACCCAG CCAGACTGGT GATAGATCTG CTGTCGTCAT CCTCTGTCCT
  301 TTTCTTCACT TCCCGTGCAA GATGGAGAAC AGCTTCCACC ACGTTGGAGC CATCTTTGGC
  361 ACTTGTTTCA CAGAATAATG CCCCATAGGT CATGGCCAGT TTTTCTCCAA GATATGCTGA
  421 TATACACTTT TGATTCTCTG CATTATCAAC ATCACGAAGA TCAGCCTTGT TTCCTACTAA
  481 CATGATAGGA ATAGTTCTGT GGGTTCCATC CTCAACCATA TCCACCCATT CTCGGACGTT
  541 AAGAAAGCTT TTCTCACAAG TAACATCGTA TAGCAGTAAG ACACCATCTG CTTTTCGGAA
  601 GTAAGACTTG GCGATACTCC TAAATCTCTC TTGCCCAGCT GTATCCCAGA GCTGAAGAAC
  661 TGTTTGTTCA CCATCCACAA TCAGAGTTTT CATTTGGAAA TCAACTCCTA GAGTTGCGCT
  721 TGTGTTCCCC TGAAACTCAT TCTTGCAGAG ACGCATGAGG AAGCTGGACT TTCCCACTGC
  781 GGCGTCGCCN GCCAGCACAA TCTTGTAAGC CTTCTGAGAT GTGANCTNGG ANGTGTTATC
  841 CACCATGTCT GTCTGAAGTG AAAGAGCTGA GATGGNCTTT CTGGAGGAAC TAAGANTGCT
  901 GNCCTCNNNN NCANANCCCN GGGGCTGNCN NNGTAANANA NANNNCNNNN TTTCANAATC
961 NAANGCTTCT TCNTCCNTTA TATCTGNNNN TNTGNNNNTG ANGCANNNNN NNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB17958_B0Cbc.seq check
>D07009A8_B0Cb_3_CMV_Forward_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNNN NAATGGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC
  121 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
  181 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
  241 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
  301 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
  361 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
  421 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
  481 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
  541 CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA
  601 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC
  661 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACNA
  721 CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT
  781 CCTGCTGGAG TTCGTGACCG CCGCCGGGNT CACTCTCGGC ATGGANGAGC TGTACNAGTC
  841 CGGANTCAGA TNCNTGGCTC AAGAGGTGTC TTCCAGTGTG NCTTTCNTCC NTAGACTGCT
  901 ATATACNGCT CTNCNTGGNC NGCTTNGAGA CAGANTGAAN CNAGGNTTGA ANAAANAANN
961 AGTNNNTANC NTGNACCNNA ANNTCACNNN NNNGGNNNNN NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, NeoR_KanR
Sequence file: RDB17958_B0Cbd.seq check
>D07009A8_B0Cb_3_SV40pro_ori_F_F06_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG NNNNNNCGNC CTCTGAGCTA TTCNNGAAGT AGTGAGGAGG
   61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC
  121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
  181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
  241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
  301 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
  361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
  421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
  481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
  541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
  601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
  661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
  721 ATGGCCGCTT TTCTGGATTC NTCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCAGG
  781 ACATAGCGTT GGCTACCCGT GATATTGCTG ANNANCTTGN CGGCGAATGG GCTGANCGCT
  841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCANCG CATCGCCTTC TATCGCCTTN
  901 TTGANGANTT CTTCTGANCG GGANTCTGGG GGNTCNAAAT GNNCGANCNA NCGNCGCCNN
  961 NNNNGNCNTC NCNANATTNN NANTNNNNCN GNNNNNNNNN ATNAANNNNN GGGNTTNNNN
1021 NNNNNTTNNN NGNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Marubashi, S., Rab7B/42 Is Functionally Involved in Protein Degradation on Melanosomes in Keratinocytes. Cell Struct. Funct. 45 (1): 45-55 (2020). PMID 32037382. [link to RRC of NBRP]