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pEGFP-C1-Rab44 (#RDB17957)

Expression vector of mouse Rab44) tagged with EGFP.

Clone info. Expression vector of mouse RAB44, member RAS oncogene family (Rab44) tagged with EGFP at N-terminus.
Comment Rab44, shorter isoform. Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells).
Gene/insert name mouse Rab44 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17957 pEGFP-C1-Rab44 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab44 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17957).

Reference section:

Marubashi, S., Fukuda, M., Rab7B/42 Is Functionally Involved in Protein Degradation on Melanosomes in Keratinocytes. Cell Struct. Funct. 45 (1): 45-55 (2020). PMID 32037382. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17957_B0Cbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5'
Sequence file: RDB17957_B0Cba.seq check
>D07009A7_B0Cb_2_EGFP-C2_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NTCNCNTGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT
   61 CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA TCCATGGCCC TCAGTGCTCA
  121 CATGCAGGAG GCCCTGGAGG CCAAGGAGCA AGAGGTTCAG CGGCTAGCAG AGGGCCAGAG
  181 AGAGCTGGAG GCTCAGCTCC TCCACCTCAG CAGCACACAG CAAGAGGCCA ACCGGGAGAA
  241 CCTGCAGCTC AGAGAGGCTG AGCGGGACCT AGCAGGGCAG CTAGAAGAGG TGCGGGGACA
  301 GCTGCAGGTG ACAAGAGGAC ACTTGGATAC TGCCAGGACT AGGGGCAAAG TGTCCTGGCA
  361 GATAGAGGAA GAGCCAAGTG TCCCCAGGGC AAATAAAGAG GCTCCAGACC CTCAGGCTGT
  421 TCCCACAGAG GAGGCCCCAC TGCCTGAGCT GTTTGGGAAT AATGACAACT GGGACCAGTT
  481 CCTCAGCAGC ATTGAAGACC ATTCCCACAG AACCCTGCGG CTTTGCTGGA GCCCACCCCC
  541 AAGCCCGAGT AGCACCTCTG CCCCCCAGAC TCCCCGCATT GTTAGACAGA TCTCCATTTC
  601 CAAGATATCT GCTCTGCAGT TTTCTCAGGA GCCAGCCTCA GATCCAGACC CGGGTCCAAG
  661 AGGCTCCCCC GAGGTGCCTC CTGGTGGTGC CAAAGATGGA AAAGGGGTGG AGGANCCCAA
  721 GGGACAGGAT GAACAGGATG TCAGCTCTAA GCAGCCTGTG GACTCCCCTG ACTCAGATGC
  781 CAGACCCAAG GGCTCTTTCC TCTGGAGCCT GCCAGGANCC CTGACCGCGG ANTCTGGGAN
  841 TGTTGAAGCA GCTTTCAGAG ATCAGCTNGC TTTTGAGGNT GAGCCNCCTC NNCANGGNNT
  901 CTCTTCCTCT NCTCAGNCCC NNGCCGGGNC CNGAAAACAG ANCNNGANTN CNNATNTGGG
  961 NGNNNAGANN NTTTNGNNCT NNNNCNNNTN NNNNNNAACN NNCCNTNANA AAGANNNNNN
1021 NNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17957_B0Cbb.seq check
>D07009A7_B0Cb_2_SV40polyA_R2_H05_23_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNNNN NNNNNNNNNN NNNNNACAAT TGCATTCNTT TTATGTTTCA
   61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
  121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT
  181 GCAGGCGGCC GCGAATTCAC TAGTGATTAT TGGACCGTGC TGGTGATCAG TGGCAGCAGC
  241 CAGCTCTCTT GGTTGATTGT GGGTGTGTCA CTTCTGCCAA TGAGGCTTTC AGGCGGTCTT
  301 CTTGCATCTT AAGTGACCGA GCCAGGTTCA TCATCGGCTC CAGGATATTG TGACCCAGGG
  361 CCGCGCTGCA CTCACCGAAG GACACCCCCA GCTCCTGGGC GAGCCTTCTG CCAGCTTCAG
  421 TGGGTACCTG CCTCTCCTCC TCACAGTCCA TCTTGTTTCC CAGCAGGACC ATGGCCACCC
  481 CCTCTACACC TGCATCCTGC AGACAGTCGA GCCAGTAACG CACGTGGGTG AAGCTCTCTT
  541 GGGAGGTGAC GTCATACATG AGCACCACCC CCTCTGCCTT GCGAAGCAGC TGTCGTGTGA
  601 GGCTGTGGTA CCTCTCTTGT CCAGCTGTGT CCCACAGTTG CAGGGCGAAG GTTTTGTTGT
  661 CTACCANCAG GTTTTTGACA CGAAAATCCA CCCCTACAGT GGNCGTCAGC CCAGTAGCAA
  721 AAGCGTCATG ATGTAGCANG TGTANGAATG ATGTCTTGCC CACATTGGAG TCCCCCAAGA
  781 ANANGACATG GTANAGGTAG TCAGGGTCAG CTTGAGGCTT GNNAGCAGTG AGGNNCCCAN
  841 GGGAAGAACT CCGTTCNNCC TNCTGNNAGT CTGTTCTTGG GTCNTCCNNN CTGCTTTCTG
  901 CNTCTGNNTC TGTGGGCTGN NNNGNACNTG ACTTGCTCTC CANCNGANNA NGNNTNGNNN
  961 NNGNNNNNGN NTGGNCTTCT GANNNNNNCN GGGNNANNNN ANNNNGNGNN NNCNNNNGGN
1021 TNNCNNNNNN NNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB17957_B0Cbc.seq check
>D07009A7_B0Cb_2_CMV_Forward_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCNAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGT CGCCACCATG
  121 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
  181 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
  241 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
  301 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG
  361 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
  421 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
  481 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
  541 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC
  601 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
  661 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
  721 CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CNTGGTCCTG
  781 CTGGAGTTCG TGANCGCCGC CNGGATCACT CTCGGNATGG ACGANCTGTA CAAGTCCGGA
  841 NNCANATCCA TGGCCCTCAG NGCTCACATG CNNNANGNCC TGGANGNNNA GGANCNAGAG
  901 GTNCNGCGGC TAGCANANGN NCNGANNNAG CTGGANGNTC AGCTCCTNCN CNNNNNNNGC
961 ANNCNNCNAG AGGNCNNNNN NNNNAANNNG CNNCTNNNNN NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, NeoR_KanR
Sequence file: RDB17957_B0Cbd.seq check
>D07009A7_B0Cb_2_SV40pro_ori_F_old_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCCNNNNTCN NCCTCTGNNC TATTCCNGAA GTAGTGAGGA
   61 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA
  781 NGACATAGCG TTGGCTACCC GTGATATTGC TGAAGANCTT GGCGGCGANN GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGTA TCGCCGCTNC CGATTCGCAN CGCATCGCCT TCTATCGCCT
  901 TCNTGANGAN TTCTTCTGAN CGGGNNNCTG GGGTTCGAAA NGANCGANCN NNNNNNNCCC
  961 NACCNNNNNT CNCNAGNNTT CNATTNCNNN NNNNGNCNNN CTATNAAAGG NNGGNNNNNN
1021 NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Marubashi, S., Rab7B/42 Is Functionally Involved in Protein Degradation on Melanosomes in Keratinocytes. Cell Struct. Funct. 45 (1): 45-55 (2020). PMID 32037382. [link to RRC of NBRP]