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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-bsr-mCherry-M-INK (#RDB17956)

M-INK, a tool for tagging mature melanosomes. Retroviral expression vector of mouse Kif1c tagged with mCherry.

Clone info. M-INK, a tool for tagging mature melanosomes. Retroviral expression vector of mouse kinesin family member 1C (Kif1c) tagged with mCherry at N-terminus.
Comment Visullization of melanosomes. Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-bsr-mCherry (plasmid)
Selectable markers Ampicillin (E. coli), Blasticidin (mammalian cells).
Gene/insert name mouse Kif1c cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested. (Ishida, M. et al. Sci. Rep. 5, 8238, 2015; Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al. Sci. Rep. 5, 8238, 2015; Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17956 pMRX-bsr-mCherry-M-INK DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMRX-bsr-mCherry-M-INK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17956).

Reference section:

Marubashi, S., Fukuda, M., Rab7B/42 Is Functionally Involved in Protein Degradation on Melanosomes in Keratinocytes. Cell Struct. Funct. 45 (1): 45-55 (2020). PMID 32037382. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17956_B0Cbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F (Pr0453)
Region: mCherry 3', insert 5'
Sequence file: RDB17956_B0Cba.seq check
>D07009A6_B0Cb_2_mCherry-C_F_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGCTG CNNGGCGCCT ACNACGTCNA CATCAAGTTG GACATCACCT
   61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
  121 CCGGCGGCAT GGACGAGCTG TACAAGTCCG GACTCGGATC TGATGATAAT GACGAGTCTG
  181 GTTTGGTCAC CTGGGCCCCA CCGGAAGGGC CAGAAGCAGT AGAGGAGACA GTGCCCAACG
  241 ACCACTCACC AGCTGTCCGG CCCACCTCAC CACCTCTGTC CAGCTGGGAG CGGGTGTCGA
  301 GATTGATGGA AGAGGACCCT GCCTTCCGTC GCGGCCGCCT TCGCTGGCTC AAGCAGGAGC
  361 AGCTGCGGCT GCAGGGACTG CAGGGCGCTG GGGGCCGGGG TGGGGGACTG CGCAGGCCTC
  421 CAGCCCGCTT TGTGCCCCCG CATGATTGCA AGCTGCGCTT CCCCTTCAAA AGCAACCCTC
  481 AGCACAGGGA GTCCTGGCCA GGAATGGGGA GTGGAGAGGC CCCAGCTCCT CAGCCCCCTG
  541 AAGAGGTCAC TGTCCCCCCA GCACCCCCTA ACCGTAGGCC TCCAAGTCCC CGAAGACCCC
  601 ACCGTTCTCG CAGGAATTCC CTAGATGGAG GGAGCCGATC TCGGGGAGGG GGTTCTACAC
  661 AGCCAGAACC CCAGCACTTA CGGCCCCAAA AGCACAACGG TTATCCCCNA CAGCCCCAGC
  721 CCAGCCCAGC CCAGCGGCCA GGGCCCCGCT ACCCACCATA CACTACCCCT CCGCGAATGC
  781 GACGGCAGCG CTCAGCCCCT GACCTCNAAG AGAGTGGGGC AGCTGTGTGA AATCACTAGT
  841 GAANTCGCGG NCGCGGTAAC NANNGNNAAC NNACTTAAGC TAGCNACGGT TTCCNNCTAG
  901 CGGGNTCNNT TNCGCCCCCC CNNCCNNNAC GTTNCNGGNN NGANNCCGCN TNNNNNNANN
961 NNNGNNNNNN GTTTGNNNNN NTGNNNNTTT NCNNNNNNNN NN
//
Primer: IRES-R (Pr0037)
Region: insert 3'
Sequence file: RDB17956_B0Cbb.seq check
>D07009A6_B0Cb_2_IRES-R_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNACG TNNNGGGGGG GGGGCGGNNN NTGANNCCCG
   61 CTAGAGGGAA ACCGTTGCTA GCTTAAGTTA GTTAACAATT GTTACCGCGG CCGCGAATTC
  121 ACTAGTGATT TCACACAGCT GCCCCACTCT CTTTGAGGTC AGGGGCTGAG CGCTGCCGTC
  181 GCATTCGCGG AGGGGTAGTG TATGGTGGGT AGCGGGGCCC TGGCCGCTGG GCTGGGCTGG
  241 GCTGGGGCTG TTGGGGATAA CCGTTGTGCT TTTGGGGCCG TAAGTGCTGG GGTTCTGGCT
  301 GTGTAGAACC CCCTCCCCGA GATCGGCTCC CTCCATCTAG GGAATTCCTG CGAGAACGGT
  361 GGGGTCTTCG GGGACTTGGA GGCCTACGGT TAGGGGGTGC TGGGGGGACA GTGACCTCTT
  421 CAGGGGGCTG AGGAGCTGGG GCCTCTCCAC TCCCCATTCC TGGCCAGGAC TCCCTGTGCT
  481 GAGGGTTGCT TTTGAAGGGG AAGCGCAGCT TGCAATCATG CGGGGGCACA AAGCGGGCTG
  541 GAGGCCTGCG CAGTCCCCCA CCCCGGCCCC CAGCGCCCTG CAGTCCCTGC AGCCGCAGCT
  601 GCTCCTGCTT GAGCCAGCGA AGGCGGCCGC GACGGAAGGC AGGGTCCTCT TCCATCAATC
  661 TCGACACCCG CTCCCAGCTG GACAGAGGTG GTGAGGTGGG CCGGACAGCT GGTGAGTGGT
  721 CGTTGGGCAC TGTCTCCTCT ACTGCTTCTG GCCCTTCCGG TGGGGGNCCA GGTGACCAAA
  781 CCAGACTCGT CATTATCATC NNATNCNAGT CNNGANTTGN ACAGCTCNTC CATGCCGCCG
  841 GNGGANTGGC GGNNNCTCGG CGCGTNCGNN CTGNNCCNCG ATGGTGTANN NNCTNGNTNG
  901 NNGGNNGTGA TGTCCAANTT GATGTTGANG TNGTANGNGN CGGGCNNCNN CNNNGGNNTT
  961 CNNGNNNTNN NNGGNGGNCT TGANCNNCNN CNNNNNANNN GNCNGNNNTN NNTNNAGCTT
1021 CANCCNNNNG NNTNNATCTC NNNNNNTNNN NNGNNNNCNN NNNNNNNN
//
Primer: MMLV-pol_F (Pr0352)
Region: mCherry 5'
Sequence file: RDB17956_B0Cbc.seq check
>D07009A6_B0Cb_2_MMLV-pol_F_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNTCNNN AGCTTGGNNA CACGCCGCCN NCGTGAAGGC
   61 TGCCGACCCC GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CCGCCGCCAC CATGGTGAGC
  121 AAGGGCGAGG AGGATAACAT GGCCATCATC AAGGAGTTCA TGCGCTTCAA GGTGCACATG
  181 GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC
  241 GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG
  301 GACATCCTGT CCCCTCAGTT CATGTACGGC TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC
  361 ATCCCCGACT ACTTGAAGCT GTCCTTCCCC GAGGGCTTCA AGTGGGAGCG CGTGATGAAC
  421 TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT CCCTGCAGGA CGGCGAGTTC
  481 ATCTACAAGG TGAAGCTGCG CGGCACCAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG
  541 AAGACCATGG GCTGGGAGGC CTCCTCCGAG CGGATGTACC CCGAGGACGG CGCCCTGAAG
  601 GGCGAGATCA AGCAGAGGCT GAAGCTGAAG GACGGCGGCC ACTACGACGC TGAGGTCAAG
  661 ACCACCTACA AGGCCAAGAA GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG
  721 TTGGACNTCA CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG
  781 GGCCGCCACT CCACCGGCGG CATGGACGAN CTGTACAAGT CCGGACTCNG ATCTGATGAT
  841 AATGACGAGT CTGGTTTGNT CACCTGGGCC CCNCCNGAAG GGCCAGAANC AGTANAGGAG
  901 ANNGTGCCCN NCGANCACTC ANCAGCTGTC CNGCCCANCT CANCNNCTCT GTCCNGCTGG
  961 GANNNNNGNC NANANTGNNG GAANANNANN NNNNNTNNNN NNNNNGNNNN NCNNNNNNNN
1021 NN
//
Primer: IRES-F (Pr0036)
Region: IRES, bsr
Sequence file: RDB17956_B0Cbd.seq check
>D07009A6_B0Cb_2_IRES-F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNNNTCGANG TTAANNNNNN TCTAGGCCCC CCGAACCNCG
   61 GGGNCGTGGT TTTCCTTTGA AAAACACGAT AATACCATGG TCATGAAAAC ATTTAACATT
  121 TCTCAACAAG ATCTAGAATT AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG
  181 GATAATAAAC ATCATGTGGG AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA
  241 GTACATATTG AAGCGTATAT AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT
  301 AGTGCAGTTT CGAATGGACA AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT
  361 TCTGACGAAG TAGATAGAAG TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG
  421 ATTTCAGACT ATGCACCAGA TTGTTTTGTG TTAATAGAAA TGAATGGCAA GTTAGTCAAA
  481 ACTACGATTG AAGAACTCAT TCCACTCAAA TATACCCGAA ATTAAAAGTC GACGATAAAA
  541 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT
  601 GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA
  661 GAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA
  721 TCTGTGGTAA GCAGTTCCTG CNCCGGCTCA NGGCCAAGAN CAGATGGAAC AGCTGAATAT
  781 GGGNCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCNNNNC AAGAACAGAT
  841 GGNCCCCANA NGNNNNCCAN CCNNNNNCAN TTTCTAGAGA NNNNTCANAN GNTTNCNNGG
  901 NGNCCCANNG ANNNNNANNG ANCNNNNNNC NTNATTTGAN NNNNNNANCN NNNCNCTNNN
961 CNNNNCNNNN NNNNNN
//
Primer: CMV enh_F1 (Pr0652)
Region: 5' LTR, psi
Sequence file: RDB17956_B0Cbe.seq check
>D07009A6_B0Cb_2_CMV enh_F1_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGCNN NNNNNNTGAC CTTATGGGAN TTTCCTACTT
   61 GGCAGTACAT CTACGTATTA GTCNTCNNTA TTACCATGCT AGAGAACCAT CAGATGTTTC
  121 CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG
  181 CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC
  241 TCACTCGGCG CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG TATCCAATAA
  301 ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA
  361 GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATTTGGGGG CTCGTCCGGG ATCGGGAGAC
  421 CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGTAAGC TGGCCAGCAA CTTATCTGTG
  481 TCTGTCCGAT TGTCTAGTGT CTATGACTGA TTTTATGCGC CTGCGTCGGT ACTAGTTAGC
  541 TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCGGAA CACCCGGCCG
  601 CAACCCTGGG AGACGTCCCA GGGACTTCGG GGGCCGTTTT TGTGGCCCGA CCTGAGTCCA
  661 AAAATCCCGA TCGTTTTGGA CTCTTTGGTG CACCCCCCTT ANAGGAGGGA TATGTGGTTC
  721 TGGTANGAGA CNANAACCTA AAACAGTTCC CGCCTCCGTC TGAATTTTTG CTTTCGGTTT
  781 GGGANCNANN NCGCGCCGCG CGTCTTGTCT GCTGCAGCAT CGTTCTGTGN TGTCTCTGTC
  841 TGACTGTGTT TCTGTANTTG TCTGAAAAAT ATGGGCCCGG NNNANACTGN NNNCACNCCN
  901 NTANNNTTGA CCTTANGTCA NTGGNNAANA NGNNNNNCGN NTCGCTCACN NCNNNNNCGG
  961 TNNANGTNNA NNAANANANN NNNGGNNNNC NTNNTGNTCT GNNNAANNNG NCCNANNNTT
1021 TNAACNNNNN GNNNNGNNNN NNNNNN
//
Primer: bsr-C_F (Pr0803)
Region: 3' LTR
Sequence file: RDB17956_B0Cbf.seq check
>D07009A6_B0Cb_2_bsr-C_F_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNNNNNNNNG AATGGCAAGT TNGTCAAAAC
   61 TACGATTGAA GAACTCATTC CACTCAAATA TACCCGAAAT TAAAAGTCGA CGATAAAATA
  121 AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGGAATGAAA GACCCCACCT GTAGGTTTGG
  181 CAAGCTAGCT TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA
  241 GAAGTTCAGA TCAAGGTCAG GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC
  301 TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGAACAG CTGAATATGG
  361 GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG
  421 TCCCCAGATG CGGTCCAGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG
  481 CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG
  541 CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG
  601 GGGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC GTGTATCCAA TAAACCCTCT
  661 TGCAGTTGCA TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC TGAGTGATTG
  721 ACTACCCGTC AGCGGGGGTC TTTCACATGC AGCATGTATC AAAATTAATT TGGTTTTTTT
  781 TCTNAAGTAT TTACNTTAAA TGGCCATAGT TGCNTTAATG ANTCGGCNNA NCGCGCGGGG
  841 AGAGGCGGTT TGCGTATTGG GGCGCTNTTC CGNTTCCTCG CTCACTGACT CNNNGCGCTC
  901 GGNCGTTCGG CTGCGGNGAN CGGTATCANC TCNCTCNAAN GNGGTAATAC NNNTNTNNNN
  961 NGANNCNGGG ATNACNCNNN NAANANNNTG TGANCAAANN NNNNNNAAAN GNNNNNNNNN
1021 N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Marubashi, S., Rab7B/42 Is Functionally Involved in Protein Degradation on Melanosomes in Keratinocytes. Cell Struct. Funct. 45 (1): 45-55 (2020). PMID 32037382. [link to RRC of NBRP]
reference Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263. [link to RRC of NBRP]