Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-bsr-Myc-M-INK (#RDB17955)

M-INK, a tool for tagging mature melanosomes. Retroviral expression vector of mouse Kif1c tagged with Myc.

Clone info. M-INK, a tool for tagging mature melanosomes. Retroviral expression vector of mouse kinesin family member 1C (Kif1c) tagged with Myc at N-terminus.
Comment Visullization of melanosomes. Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-bsr-Myc (plasmid)
Selectable markers Ampicillin (E. coli), Blasticidin (mammalian cells).
Gene/insert name mouse Kif1c cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested. (Ishida, M. et al. Sci. Rep. 5, 8238, 2015; Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al. Sci. Rep. 5, 8238, 2015; Marubashi, S. et al. Cell Struct. Funct. doi: 10.1247/csf.19039, 2020)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17955 pMRX-bsr-Myc-M-INK DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMRX-bsr-Myc-M-INK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17955).

Reference section:

Marubashi, S., Fukuda, M., Rab7B/42 Is Functionally Involved in Protein Degradation on Melanosomes in Keratinocytes. Cell Struct. Funct. 45 (1): 45-55 (2020). PMID 32037382. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17955_B0Cbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F2 (Pr0364)
Region: Myc, insert 5'
Sequence file: RDB17955_B0Cba.seq check
>D07009A5_B0Cb_3_MMLV-pol_F2_F05_17_ABI24.ab1
    1 NNNNNNGNNN NNNNNNCNNN NNGNNNGCCG GNNCNGCTTG GCATGGAGCA AAAGCTCATT
   61 TCTGAAGAGG ACTTGACGCG TGATTATGAT ATTCCAACTA CTGCTAGCGA GAATTTGTAT
  121 TTTCAGGGTG AGCTCGGATC TGATGATAAT GACGAGTCTG GTTTGGTCAC CTGGGCCCCA
  181 CCGGAAGGGC CAGAAGCAGT AGAGGAGACA GTGCCCAACG ACCACTCACC AGCTGTCCGG
  241 CCCACCTCAC CACCTCTGTC CAGCTGGGAG CGGGTGTCGA GATTGATGGA AGAGGACCCT
  301 GCCTTCCGTC GCGGCCGCCT TCGCTGGCTC AAGCAGGAGC AGCTGCGGCT GCAGGGACTG
  361 CAGGGCGCTG GGGGCCGGGG TGGGGGACTG CGCAGGCCTC CAGCCCGCTT TGTGCCCCCG
  421 CATGATTGCA AGCTGCGCTT CCCCTTCAAA AGCAACCCTC AGCACAGGGA GTCCTGGCCA
  481 GGAATGGGGA GTGGAGAGGC CCCAGCTCCT CAGCCCCCTG AAGAGGTCAC TGTCCCCCCA
  541 GCACCCCCTA ACCGTAGGCC TCCAAGTCCC CGAAGACCCC ACCGTTCTCG CAGGAATTCC
  601 CTAGATGGAG GGAGCCGATC TCGGGGAGGG GGTTCTACAC AGCCAGAACC CCAGCACTTA
  661 CGGCCCCAAA AGCACAACGG TTATCCCCAA CAGCCCCAGC CCAGCCCAGC CCAGCGGCCA
  721 GGGCCCCGCT ACCCACCATA CACTACCCCT CCGCGAATGC GACGGCAGCG CTCAGCCCCT
  781 GNNCTCAAAG AGAGTGGGGC AGCTGTGTNN NNCACTAGTG AATTCGCGGC CGCGGTAACA
  841 ATTGTTAACT AACTTAAGCT AGCAACGGTT TCCCTCTANC GGGNTCAATT NCGCCCCCCC
  901 CCCCCNNNAC GTTACTGGNC NAANCCGCTT NNNANNAANG NNNGGNGTGC GTTTGTCTAT
961 NTGNTNNTTT TCCACCNTAN NGNNGNNNTT NNNNNNNGNN NNNNNNNN
//
Primer: IRES-R (Pr0037)
Region: IRES, insert 3'
Sequence file: RDB17955_B0Cbb.seq check
>D07009A5_B0Cb_3_IRES-R_F04_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TNGGNNNNNG GGGCGGAATT GATCCCGCTA
   61 GAGGGAAACC GTTGCTAGCT TAAGTTAGTT AACAATTGTT ACCGCGGCCG CGAATTCACT
  121 AGTGATTTCA CACAGCTGCC CCACTCTCTT TGAGGTCAGG GGCTGAGCGC TGCCGTCGCA
  181 TTCGCGGAGG GGTAGTGTAT GGTGGGTAGC GGGGCCCTGG CCGCTGGGCT GGGCTGGGCT
  241 GGGGCTGTTG GGGATAACCG TTGTGCTTTT GGGGCCGTAA GTGCTGGGGT TCTGGCTGTG
  301 TAGAACCCCC TCCCCGAGAT CGGCTCCCTC CATCTAGGGA ATTCCTGCGA GAACGGTGGG
  361 GTCTTCGGGG ACTTGGAGGC CTACGGTTAG GGGGTGCTGG GGGGACAGTG ACCTCTTCAG
  421 GGGGCTGAGG AGCTGGGGCC TCTCCACTCC CCATTCCTGG CCAGGACTCC CTGTGCTGAG
  481 GGTTGCTTTT GAAGGGGAAG CGCAGCTTGC AATCATGCGG GGGCACAAAG CGGGCTGGAG
  541 GCCTGCGCAG TCCCCCACCC CGGCCCCCAG CGCCCTGCAG TCCCTGCAGC CGCAGCTGCT
  601 CCTGCTTGAG CCAGCGAAGG CGGCCGCGAC GGAAGGCAGG GTCCTCTTCC ATCAATCTCG
  661 ACACCCGCTC CCAGCTGGAC AGAGGTGGTG AGGTGGGCCG GACAGCTGGT GAGTGGTCGT
  721 TGGGCACTGT CTCCTCTACT GCTTCTGGCC CTTCCGGTGG GGCCCAGGTG ANCAAACCAN
  781 ACTCGTCATT ATCATCAGAT CCGANCTCAC CCTGAAAATA CNAATTCTCN NNNNCAGTAG
  841 TTGGAATATC ATAATCACGC NNCAAGNNCN TCTTCAGAAA TGANNTTTTG NTCCATGCCN
  901 AGCNNATNCG GCANTCTANA NGNANGGTCC ACCCCCGGGG TCNGNCNGCC TNNNNGNGGG
  961 CGGCNTGNAT CCNANCTGCN ANGCCGNCTA NTTTGNNGGN NNGNNGGGGG GNNGNNNNNN
1021 NNGANNNNGN NNNNNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES, bsr
Sequence file: RDB17955_B0Cbc.seq check
>D07009A5_B0Cb_3_IRES-F_G04_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNAAA AACGTCTAGG CCCCCCGAAC
   61 CNCGGGGACG TGGTTTTCCT TTGAAAAACA CGATAATACC ATGGTCATGA AAACATTTAA
  121 CATTTCTCAA CAAGATCTAG AATTAGTAGA AGTAGCGACA GAGAAGATTA CAATGCTTTA
  181 TGAGGATAAT AAACATCATG TGGGAGCGGC AATTCGTACG AAAACAGGAG AAATCATTTC
  241 GGCAGTACAT ATTGAAGCGT ATATAGGACG AGTAACTGTT TGTGCAGAAG CCATTGCGAT
  301 TGGTAGTGCA GTTTCGAATG GACAAAAGGA TTTTGACACG ATTGTAGCTG TTAGACACCC
  361 TTATTCTGAC GAAGTAGATA GAAGTATTCG AGTGGTAAGT CCTTGTGGTA TGTGTAGGGA
  421 GTTGATTTCA GACTATGCAC CAGATTGTTT TGTGTTAATA GAAATGAATG GCAAGTTAGT
  481 CAAAACTACG ATTGAAGAAC TCATTCCACT CAAATATACC CGAAATTAAA AGTCGACGAT
  541 AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG
  601 GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAAATA CATAACTGAG
  661 AATAGAGAAG TTCAGATCAA GGTCAGGAAC NNNTGGAACA GCTGAATATG GGCCAAACAG
  721 GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCANGGCCA AGANCNGATG GAACAGCTGA
  781 NTATGGGCNA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCNN NGCCAAGAAC
  841 ANANGGTCCC CAGATGCGGT CCAGCCCTCA NCAGNTNCTN NANNNNCNTC NNATGTTTNC
  901 NNGGTGCCCC NANGGANCTG AAATGANCCT GTGCCNTATT TGAACTAACN NATCAGTTCG
  961 CTTNTNGCTN CTGNTCNNGC GCNTCNGNTC CCCGAGCTNN NNNAAANANC NNNNANNCCN
1021 NNNNNNNNN
//
Primer: CMV enh_F1 (Pr0652)
Region: 5' LTR, psi
Sequence file: RDB17955_B0Cbd.seq check
>D07009A5_B0Cb_3_CMV enh_F1_H04_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNNNGNN NNTGANCTTA TGGGACTTTN NNNNNNGGCA
   61 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGCTAGAG AACCATCAGA TGTTTCCAGG
  121 GTGCCCCAAG GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC
  181 TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC AACCCCTCAC
  241 TCGGCGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC CAATAAACCC
  301 TCTTGCAGTT GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA
  361 TTGACTACCC GTCAGCGGGG GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT
  421 GCCCAGGGAC CACCGACCCA CCACCGGGAG GTAAGCTGGC CAGCAACTTA TCTGTGTCTG
  481 TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA GTTAGCTAAC
  541 TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT TCGGAACACC CGGCCGCAAC
  601 CCTGGGAGAC GTCCCAGGGA CTTCGGGGGC CGTTTTTGTG GCCCGACCTG AGTCCAAAAA
  661 TCCCGATCGT TTTGGACTCT TTGGTGCACC CCCCTTAGAG GAGGGATATG TGGTTCTGGT
  721 AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGGA
  781 NCGAAGCCGC GCCGCGCGTC TTGTCTGCTG CANCATCGTT CTGTGTTGTC TCTGTCTGAC
  841 TGTGTTTCTG TATTTGTCTG AAAATATGGG NCCGGGCCAN ANTGNNNCCN CTNCCTTAAG
  901 TTTGANCTTN NGTCANTGGA AANANGNCNA NCGGNTCGNT NNCNACCNGN CNGTANNNGT
961 CAAGANNANN NGTTNGGGNT ANCNTNNGNT CTNNNNAANN NNNNNNNN
//
Primer: bsr-C_F (Pr0803)
Region: 3' LTR
Sequence file: RDB17955_B0Cbe.seq check
>D07009A5_B0Cb_3_bsr-C_F_A05_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN AANNNNTTCC NCTNNNATAT ACCCGAAATT
   61 NNNANTCGAC GATAAAATAN NNNNNTTTAT TTANTCTCCN GAAAAAGGGG GGAATGAAAG
  121 ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC NTTTTGCAAG GCATGGAAAA
  181 ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTCAGG AACAGATGGA ACAGCTGAAT
  241 ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG
  301 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  361 CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC TCAGCAGTTT CTAGAGAACC
  421 ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA
  481 CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA
  541 GCCCACAACC CCTCACTCGG GGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG
  601 TGTATCCNAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG
  661 GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCNNNTGNN NNATGTATCA
  721 AAATTANNTT GGNTTTTTTT CTNNANGTAT TTACATTAAA ATGGCCATAG TTGCATTAAT
  781 GAANTCGGCC NAACGCGCGG GGNANANGCG GTTTGCNTAT TGGGCGCTCT TCCGCTTCCN
  841 CGCTCACNGA CTCGCTGCGC TCGGNCGTNN GNNTGCNGCN ANCGNNATCA NCTCACTCNA
  901 AGGNNGTAAT ACNGNTATCC NCAGANTCNN GGNANNNCGC NNNNNANNNN NNNTGANCNA
  961 NNNGNCANNN AANGGNNNNG NANNNNNAAA ANNNCNGCGT TGCTNGNNNN TTTTTNNNNN
1021 GNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Marubashi, S., Rab7B/42 Is Functionally Involved in Protein Degradation on Melanosomes in Keratinocytes. Cell Struct. Funct. 45 (1): 45-55 (2020). PMID 32037382. [link to RRC of NBRP]
reference Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263. [link to RRC of NBRP]