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pMRX-puro-EGFP-DENND1A

Retroviral expression vector of mouse Dennd1a) tagged with EGFP.

Catalog number RDB17952
Resource name pMRX-puro-EGFP-DENND1A
Clone info. Retroviral expression vector of mouse DENN/MADD domain containing 1A (Dennd1a) tagged with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pMRX-puro-EGFP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cells).
Gene/insert name mouse Dennd1a cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
mouse Dennd1a
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested. (Fukuda, M. et al. Cell Struct. Funct. 36, 155-170, 2011; Kinoshita, R. et al. J. Biol. Chem. doi:10.1074/jbc.RA119.011646, 2020)
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al. Cell Struct. Funct. 36, 155-170, 2011; Kinoshita, R. et al. J. Biol. Chem. doi:10.1074/jbc.RA119.011646, 2020)
          付加的提供条件：
          pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB17952 pMRX-puro-EGFP-DENND1A Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Kinoshita, R., Rab35-GEFs, DENND1A and folliculin differentially regulate podocalyxin trafficking in two- and three-dimensional epithelial cell cultures. J. Biol. Chem. 295 (11): 3652-3663 (2020). PMID 31992598.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Kinoshita, R., Rab35-GEFs, DENND1A and folliculin differentially regulate podocalyxin trafficking in two- and three-dimensional epithelial cell cultures. J. Biol. Chem. 295 (11): 3652-3663 (2020). PMID 31992598.
          reference Fukuda, M., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958.

          2022.10.12

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