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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

spret SLGE-luc2 (#RDB17901)

Luciferase reporter of the Western Mediterranean mouse (Mus spretus) Shh enhancer, SLGE.

Clone info. Luciferase reporter of the Western Mediterranean mouse (Mus spretus) Sonic hedgehog (Shh) enhancer, SLGE.
Comment Expression was confirmed by the depositor with luciferase assay.
Vector backbone pGL4.23 (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Ampicillin
Gene/insert name mouse Shh genomic
Depositor|Developer Amano, Takanori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tsukiji N. et al., Mech. Dev. 131:127-36, 2014)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17901 spret SLGE-luc2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The spret SLGE-luc2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17901).

Reference section:

Tsukiji, N., Amano, T., Shiroishi, T., A novel regulatory element for Shh expression in the lung and gut of mouse embryos. Mech. Dev. 131: 127-136 (2014). PMID 24157522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17901_B0Afp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: Pause site, spret SLGE 5'
Sequence file: RDB17901_B0Afa.seq check
>D06932A6_B0Af_2_pGL4ins_F_H11_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGGCTG TCCCCNGTGC AAGTGCAGGT GCCAGAACAT
   61 TTCTCTGGCC TAACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGGCCAAG GAGGACTATG
  121 TTTATTTAGA TGAGAAGAGG AAGACGTGCT GGGCATCAGT GCATTCTGTG TGTTTCAGGC
  181 ATGACTGTGG TGTATAATGG TGTGTAGAAC ACTGCAAGAC ACTTAGAACA GAGAATGGTT
  241 ATTAAAGGTT ATAGAAATGC TAATTATCTT GACTTGATCC TCAAATGTTT TCATGCACCA
  301 AAACTTGTCT CTTAAGTGCA TAAAAACATT ATAAAATAAT AATAATAAAA ATAATAGAAG
  361 TCAGCCACCC TCATTGCTGC CACCACAAGC ACATTCATCT CCTAGACCTC CTCAGCACTG
  421 AAAACTTCTC CTCCTCCCTT CCCCCTTCTC CCCATCTTCT TCTCCTTCTC TTCCTCCTCC
  481 CCTTCCTCCC CTTCCTCCTC CTCCTCCACT ACAACCTTCC TATGATCAGT GCTTCTTGCA
  541 AGTAATCTGG CCTAAATGTA GGTGTCTCTT GCCCTTCTAG AATGTGCTCT TCAGTACAGA
  601 ATGACTTAGG CCATTGTGCC CACATGGATG CTGTAGAAAG TTTCTACACA GTGGAGAAAG
  661 CTCTAGATTA AGCACCCCTC TTTTGGAAGA ACCATCTGGA AAGGTCATGT GCCAGGCCAC
  721 TGGAGATCAC TCAAGCCTCA CAGGCCCAGC AGCTCTTCCT GAGAACCTGT TGTGCTACAC
  781 GGGGACAACT CTGCTGCAGC CCATTTCTGC AGTGTGTCCT TGGGCCAGCA CTCCCCTCTC
  841 ACATTCCTCA GTCTTTGCTG GTGCACTTTC ATGTTTGNCN AANTCCNAGG NCTTCCTCCC
  901 TCCCCTCTNN NCTTTGTCNN NNNGGTTCCC ANAANNGNCN ACTTCCCNNN CCCNCCCCCN
  961 CCNNNNCNNN GGNNTNNNNN NNNCCCNNCN NNNNNCNNNN NCNNNNNNNG NTANCNCAGC
1021 TNCNGGGGNA NNNNNNANNN NNTNNNNNNN CNNNNNNNNN N
//
Primer: mSLGE_F1 (Pr0811)
Region: spret SLGE
Sequence file: RDB17901_B0Afb.seq check
>D06932A6_B0Af_2_mSLGE_F1_F08_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGCC NCNNNGGCCN NGCAGCTCTT CCTGAGAANC TGTTGTGCTA
   61 CACGGGGNCA ACTCTGCTGC AGCCCATTTC TGCAGTGTGT CCTTGGGCCA GCACTCCCCT
  121 CTCACATTCC TCAGTCTTTG CTGGTGCACT TTCATGTTTG CCTAACTCCA AGGCCTTCCT
  181 CCCTCCCCTC TTGGCTTTGT CTTAAGGGTT CCCAGAAGGT CAACTTCCCT TGCCCTCCCC
  241 CACCTCTCCA GTGGAGTAGG CACAACCCCA CTGATAACAC ATCCTTCTAT GTTAGCACAG
  301 CTCCAGGGGC AAAGAGCAAA GGCTTAACCA CCCAAACAAC TCTGGGTGAG CAAACTCTGC
  361 ACTAAAGACA TTCAGGGGCC AAAGACCTTC CAGGCTCAGA GCCACTCATT GAGGAGTGGA
  421 GATAAAACAG TCAGTCGGGG TCCCAGCCAC TGCATGGGAT TCTTTGTATC TCACAGTTGG
  481 CCCCAGAAAG GCCCCTTTAT AGAGGGCCAG GGCTGTATTA AAGGCAAGGA AAAAATGTCT
  541 CTCAGCCCCC AAAAGTAAAC CACTCATGAA AGTACAGAAT TTTACAGGGG GATTTACTTC
  601 CTTCTTATCT CCACAGAGGA AGGGAAGGCA AGGCTTGGGC AGGCAGACAG GTGCTGCATT
  661 CCTCAGAGAA GCAGGTGGGG AGTCTGTAGC ACTGGTGCCG AGCCGCCTCC CACCCTGCAC
  721 ACCGAGCTGC ANGCAGTAAA CAGGTGGCAG GGACATTGGA GCCAGANGTG GATCTCTCAC
  781 CACTGCTTGC TACCAGGTAT TATCCACTGA TTCTGAAAAA TCACATTCTC CGTCATTCAG
  841 NGNANANGGG GNGANATGGN NATCATAAGC CNAAAATGAG ANNACAAATG TTNACNAANT
901 CCTTANNCNN GAAANANGNT CCCNNNNANT CTTCNNNGNN NNNNNNNN
//
Primer: Luc_R (Pr0146)
Region: luc2 5', mini P, spret SLGE 3'
Sequence file: RDB17901_B0Afc.seq check
>D06932A6_B0Af_2_Luc_R_A12_03_ABI24.ab1
    1 NNNNNNNNNN NGGNNNNNNN GGCGTAGCGC TTCNTGGCTT TGTGCNGCTG CTCGCCGGCG
   61 GTCCCGTCTT CGAGTGGGTA GAATGGCGCT GGGCCCTTCT TAATGTTTTT GGCATCTTCC
  121 ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTGGAA GTCGAGCTTC CATTATATAC
  181 CCTCTAGTGT CTAAGCTTAA ATGCCCATGC CAAGGGGAGC AGCCCACTCA TCCTGGTTTT
  241 CCTGAACCTT GTGGTTTCAT CACAAAAGTC TTGTATCCTG GGATGCTTTA CAGTCCAGTG
  301 TTCTTAGTCT CACATTACAG GACAGGTTGA GATTTGTTCT TGACCTTTTT CACTAGAGAG
  361 CCTTGGAGTG CATTGAAGAT TTATTGGGAA CCTCTTTCAG GGCTAAGGAT TTTGTAAACA
  421 TTTGTAGTCT CATTTTAGGC TTATGATGTC CATATCACCC CATCTCCACT GAATGACGGA
  481 GAATGTGATT TTTCAGAATC AGTGGATAAT ACCTGGTAGC AAGCAGTGGT GAGAGATCCA
  541 CCTCTGGCTC CAATGTCCCT GCCACCTGTT TACTGCCTGC AGCTCGGTGT GCAGGGTGGG
  601 AGGCGGCTCG GCACCAGTGC TACAGACTCC CCACCTGCTT CTCTGAGGAA TGCAGCACCT
  661 GTCTGCCTGC CCAAGCCTTG CCTTCCCTTC CTCTGTGGAG ATAAGAAGGA AGTAAATCCC
  721 CCTGTAAAAT TCTGTACTTT CATGAGTGGT TTACTTTTGG GGGCTGANAG ACATTTTTTC
  781 CTTGCCTTTA ATACAGCCCT GGCCCTCTAT AAAGGGGCCT TTCTGGNGCC NACTGTGANA
  841 TACAAAGAAT CCCATGCAGT GGCTGGGANC CCGACTGACT GTTTTATCTC CACTCCTCNA
  901 NGAGTGGCTC TGANNCTGGA ANGNCTTTGG CCCCNGAATG TCTTTAGTGC NNANTTTGNT
  961 CACCCNNANT TGTTTGGGNG GNTNANCCTT NGNTCTTNGN CCCNNNNNCT GNGCTNACAT
1021 ANANNNNGNN NNNTCANNGG GGNNNNNNNN ANTNNNNNNN NNN
//
Primer: pGL4pA_R (Pr0664)
Region: SV40 late p(A), luc2 3'
Sequence file: RDB17901_B0Afd.seq check
>D06932A6_B0Af_2_pGL4pA_R_B12_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTNNTCNN NNTTNNCNNC NTTTGTAGAG GTTTTACTTG
   61 CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG
  121 TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT
  181 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG
  241 TATCTTATCA TGTCTGCTCG AAGCGGCCGG CCGCCCCGAC TCTAGAATTA TTACACGGCG
  301 ATCTTGCCGC CCTTCTTGGC CTTAATGAGA ATCTCGCGGA TCTTGCGGGC GTCCAACTTG
  361 CCGGTCAGTC CTTTAGGCAC CTCGTCCACG AACACAACAC CACCGCGCAG CTTCTTGGCG
  421 GTTGTAACCT GGCTGGCCAC ATAGTCCACG ATCTCCTTCT CGGTCATGGT TTTACCGTGT
  481 TCCAGCACGA CGACNGCGGC GGGCAGCTCG CCGGCATCGT CGTCGGGCAG GCCGGCGACC
  541 CCGGCGTCGA AGATGTTGGG GTGTTGCAGC AGGATGCTCT CCAGTTCGGC TGGGGCTACC
  601 TGGTAGCCCT TGTATTTGAT CAGGCTCTTC AGCCGGTCCA CGATGAAGAA GTGCTCGTCC
  661 TCGTCCCAGT AGGCGATGTC GCCGCTGTGC AGCCAGCCGT CCTTGTCGAT GAGAGCGTTT
  721 GTAGCCTCGG GGTTGTTAAC GTAGCCGCTC ATGATCATGG GGCCACGGAC GCACAGCTCG
  781 CCGCGCTGGT TCACACCCAG TGTCTTACCG GTGTCCAAGT CCACCACCTT AGCCTCNAAG
  841 AAGGGCACCA CCTTGCCTAC TGCGCCNGGC TTGNCGTCCC CTTCNGGGGN GANCNNAANG
  901 NNGCTGGTTG TTTCTGTCNN NNCGTANCCC NNNNNNANGN CTGGNNNNNN GAANCGNTNG
  961 GNCNNGGNCT CNNCNACCTC CNNGNTGANC NGNGCCCCGN CNCNNGNNNN NTCNNGNNAG
 1021 TTGNTTNNNN CGTNNNNNNN NANNANANNG NTNNTNNNNN NNNANNNNAA TNNNNNNGNN
1081 NNNNNCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsukiji, N., A novel regulatory element for Shh expression in the lung and gut of mouse embryos. Mech. Dev. 131: 127-136 (2014). PMID 24157522. [link to RRC of NBRP]