RDB17750 - pN1-TOMM20-Halo

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pN1-TOMM20-Halo (#RDB17750)

Halo Tag, Mitochondrial outer membrane localization

Alternative name	(stock no. 1131)
Clone info.	Halo Tag, Mitochondrial outer membrane localization (TOMM20). CMV promoter.
Comment	Expression was confirmed by the depositor in mammalian cell.
Vector backbone	pN1 (plasmid)
Gene/insert name	Rhodococcus rhodochrous dehalogenase genomic DNA human TOMM20 cDNA
Depositor\|Developer	Okada, Yasushi \| Yaginuma, Hideyuki \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Wang et al, Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Wang et al, Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Wang et al, Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) 。本件リソースは非営利機関の学術研究目的にのみ利用することができる。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Wang et al, Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) 。本件リソースは非営利機関の学術研究目的にのみ利用することができる。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB17750	pN1-TOMM20-Halo	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pN1-TOMM20-Halo was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17750).

Reference section:

Wang, C., Taki, M., Sato, Y., Tamura, Y., Yaginuma, H., Okada, Y., Yamaguchi, S., A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae. Proc. Natl. Acad. Sci. U.S.A. 116 (32): 15817-15822 (2019). PMID 31337683. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17750_A9L3p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'(TOMM20,Halo tag 5') Sequence file: RDB17750_A9L3a.seq
>D06362C2_A9L3_3_CMV_Forward_D03_12_ABI24.ab1 1 NNNNNNNNNN NNCNNNNNNT NNNNNNNNNT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA 61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGA CTCAGATCTC 121 GAGCTCAAGC TTCGAATTCG CCACCATGGT GGGTCGGAAC AGCGCCATCG CCGCCGGTGT 181 ATGCGGGGCC CTTTTCATTG GGTACTGCAT CTACTTCGAC CGCAAAAGAC GAAGTGACCC 241 CAACTTCAAG AACAGGCTTC GAGAACGAAG AAAGAAACAG AAGCTTGCCA AGGAGAGAGC 301 TGGGCTTTCC AAGTTACCTG ACCTTAAAGA TGCTGAAGCT GTTCAGAAGT TCTTCCTTGA 361 AGAAATACAG CTTGGTGAAG AGTTACTAGC TCAAGGTGAA TATGAGAAGG GCGTAGACCA 421 TCTGACAAAT GCAATTGCTG TGTGTGGACA GCCACAGCAG TTACTGCAGG TCTTACAGCA 481 AACTCTTCCA CCACCAGTGT TCCAGATGCT TCTGACTAAG CTCCCAACAA TTAGTCAGAG 541 AATTGTAAGT GCTCAGAGCT TGGCTGAAGA TGATGTGGAA GGCGGTAGCG GGGATCCACC 601 GGTCTCGACC GGCAGCGAAA TCGGTACTGG CTTTCCATTC GACCCCCATT ATGTGGAAGT 661 CCTGGGCGAG CGCATGCACT ACGTCGATGT TGGTCCGCGC GATGGCACCC CTGTGCTGTT 721 CCTGCACGGT AACCCGACCT CCTCCTACGT GTGGNGCAAC ATCATCCCGC ATGTTGCACC 781 GACCCATCGC TGCATTGCTC CAGACCTGAT CGGTATGGGC AAATCCGACA AACCAGANCT 841 GGGNTANNNC TTCGACGACC ACGTCCGCTN CATGGATGCN TTCNTCGANN NCCTGGGNCT 901 NGNAAGANGN CNNCCNNNNN NTTCNCNNNN GGGNNCTNCN NNNTGGGTTT NNNNNNNNNN //
Primer: pAxEF R2 (Pr0059) Region: SV40 pA,insert 3'(Halo tag 3') Sequence file: RDB17750_A9L3b.seq
>D06362C2_A9L3_3_pAxEF R2_E03_15_ABI24.ab1 1 NNNNNNNNNN GNTTNNNTTG TGAANTTGTG NNGCTATTGC TTTATTTGTA ACCATTATAA 61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG 121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG 181 ATCTAGAGTC GCGGCCGCTT AACCGGAAAT CTCCAGAGTA GACAGCCAGC GCGCGATCTC 241 GCTGCCGATC AGGTCCGGGT TGTCTTCTTG CAGCAGATTC AGACCCGGGC CGATGTCCAC 301 AGCCTTGCAG TTAGGCAGGC TTTTGGCCAG GCGAGCGGCT TCGGCCGGTG GGATCAGAAC 361 GCCTGGGGTG CCCCAGAACA GCAGCTTCGG GACAGGGGAC TGGTGCAGCC AGTCCATGTA 421 TTCTTCGACC AGCGCGACGA TGTTCGCTGG CTCACCGGCG ATTGGCAGCT CGTTTGGGAA 481 GCGCCACAGT GGCTCGCGGT CAACAGGATT CAGGAACGGC TCGCGGTAAT GGTCCATCTC 541 GACTTCAGTC AGCGGGCGGA CGACACCCAT CGGCAGCGTA CCCTCGATAA AAACGTTCTG 601 ATCGATGATC AGCTTGCGGC CGACGTCGGT GGTGCGGAAG GCCTGGAAGG TCTCGCGGGC 661 AAATTCTGGC CATTCGTCCC AGGTCGGGAT AGGGCGGATG AACTCCATAA ATGCAATACC 721 TTTGACGCGC TCTGGATTGC GCTTGGCCCA GTGGANNCCC AGANCGGANC CCCAGTCGTG 781 AATGACCAGG ACGACCTCTT CCAGANCCAN GGCTTCNATG AANNNNTCCA TGAANCGGAN 841 GTGGTCGNCN NANAAATAAC CCANGTCTGG NTNGNCGNAT TNGCCCNTAC CGATCANGNC 901 TNGANCNANG CANCGATNGG NNCGGNNNNN CNTGCNGGNN GNTGTNNNNN NNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB17750_A9L3c.seq
>D06362C2_A9L3_3_SV40pro_ori_F_F03_18_ABI24.ab1 1 NNNNNNNNNN NNNNCNNNNN NNNNNNNNGC CTCTGANCTA TTCNNGAAGT AGTGAGGANG 61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC 121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT 181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT 241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC 301 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG 361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC 421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA 481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC 541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG 601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG 661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA 721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCANG 781 ACATAGCGTT GGCTANCCGT GATATTGCTG AAGAGCTTGN CGGCGANTGG GCTGACCGCT 841 TCCTCGNGCT TTACNGTATC GCCGCTCCCN ATTCGCANCG CATCNCCTTC TATCGCCTNC 901 NTGANNANTT CTTCTGANCG GGNNTCTNGG NNNTNCNAAA TGANNNNNCN AAGCGNNNNC 961 NNANNCNNNN CNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Wang, C., A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae. Proc. Natl. Acad. Sci. U.S.A. 116 (32): 15817-15822 (2019). PMID 31337683. [link to RRC of NBRP]