Please review the QC test results indicated by icon below as well as clone information before placing your order.

pN1-TOMM20-Halo

Halo Tag, Mitochondrial outer membrane localization

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Wang et al, Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Wang et al, Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) 。本件リソースは非営利機関の学術研究目的にのみ利用することができる。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17750_A9L3p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,insert 5'(TOMM20,Halo tag 5') Sequence file: RDB17750_A9L3a.seq
>D06362C2_A9L3_3_CMV_Forward_D03_12_ABI24.ab1     1 NNNNNNNNNN NNCNNNNNNT NNNNNNNNNT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA    61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGA CTCAGATCTC   121 GAGCTCAAGC TTCGAATTCG CCACCATGGT GGGTCGGAAC AGCGCCATCG CCGCCGGTGT   181 ATGCGGGGCC CTTTTCATTG GGTACTGCAT CTACTTCGAC CGCAAAAGAC GAAGTGACCC   241 CAACTTCAAG AACAGGCTTC GAGAACGAAG AAAGAAACAG AAGCTTGCCA AGGAGAGAGC   301 TGGGCTTTCC AAGTTACCTG ACCTTAAAGA TGCTGAAGCT GTTCAGAAGT TCTTCCTTGA   361 AGAAATACAG CTTGGTGAAG AGTTACTAGC TCAAGGTGAA TATGAGAAGG GCGTAGACCA   421 TCTGACAAAT GCAATTGCTG TGTGTGGACA GCCACAGCAG TTACTGCAGG TCTTACAGCA   481 AACTCTTCCA CCACCAGTGT TCCAGATGCT TCTGACTAAG CTCCCAACAA TTAGTCAGAG   541 AATTGTAAGT GCTCAGAGCT TGGCTGAAGA TGATGTGGAA GGCGGTAGCG GGGATCCACC   601 GGTCTCGACC GGCAGCGAAA TCGGTACTGG CTTTCCATTC GACCCCCATT ATGTGGAAGT   661 CCTGGGCGAG CGCATGCACT ACGTCGATGT TGGTCCGCGC GATGGCACCC CTGTGCTGTT   721 CCTGCACGGT AACCCGACCT CCTCCTACGT GTGGNGCAAC ATCATCCCGC ATGTTGCACC   781 GACCCATCGC TGCATTGCTC CAGACCTGAT CGGTATGGGC AAATCCGACA AACCAGANCT   841 GGGNTANNNC TTCGACGACC ACGTCCGCTN CATGGATGCN TTCNTCGANN NCCTGGGNCT   901 NGNAAGANGN CNNCCNNNNN NTTCNCNNNN GGGNNCTNCN NNNTGGGTTT NNNNNNNNNN //
Primer: pAxEF R2 Region: SV40 pA,insert 3'(Halo tag 3') Sequence file: RDB17750_A9L3b.seq
>D06362C2_A9L3_3_pAxEF R2_E03_15_ABI24.ab1     1 NNNNNNNNNN GNTTNNNTTG TGAANTTGTG NNGCTATTGC TTTATTTGTA ACCATTATAA    61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG   121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG   181 ATCTAGAGTC GCGGCCGCTT AACCGGAAAT CTCCAGAGTA GACAGCCAGC GCGCGATCTC   241 GCTGCCGATC AGGTCCGGGT TGTCTTCTTG CAGCAGATTC AGACCCGGGC CGATGTCCAC   301 AGCCTTGCAG TTAGGCAGGC TTTTGGCCAG GCGAGCGGCT TCGGCCGGTG GGATCAGAAC   361 GCCTGGGGTG CCCCAGAACA GCAGCTTCGG GACAGGGGAC TGGTGCAGCC AGTCCATGTA   421 TTCTTCGACC AGCGCGACGA TGTTCGCTGG CTCACCGGCG ATTGGCAGCT CGTTTGGGAA   481 GCGCCACAGT GGCTCGCGGT CAACAGGATT CAGGAACGGC TCGCGGTAAT GGTCCATCTC   541 GACTTCAGTC AGCGGGCGGA CGACACCCAT CGGCAGCGTA CCCTCGATAA AAACGTTCTG   601 ATCGATGATC AGCTTGCGGC CGACGTCGGT GGTGCGGAAG GCCTGGAAGG TCTCGCGGGC   661 AAATTCTGGC CATTCGTCCC AGGTCGGGAT AGGGCGGATG AACTCCATAA ATGCAATACC   721 TTTGACGCGC TCTGGATTGC GCTTGGCCCA GTGGANNCCC AGANCGGANC CCCAGTCGTG   781 AATGACCAGG ACGACCTCTT CCAGANCCAN GGCTTCNATG AANNNNTCCA TGAANCGGAN   841 GTGGTCGNCN NANAAATAAC CCANGTCTGG NTNGNCGNAT TNGCCCNTAC CGATCANGNC 901 TNGANCNANG CANCGATNGG NNCGGNNNNN CNTGCNGGNN GNTGTNNNNN NNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB17750_A9L3c.seq
>D06362C2_A9L3_3_SV40pro_ori_F_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNCNNNNN NNNNNNNNGC CTCTGANCTA TTCNNGAAGT AGTGAGGANG    61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC   121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT   181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT   241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC   301 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG   361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC   421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA   481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC   541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG   601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG   661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA   721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCANG   781 ACATAGCGTT GGCTANCCGT GATATTGCTG AAGAGCTTGN CGGCGANTGG GCTGACCGCT   841 TCCTCGNGCT TTACNGTATC GCCGCTCCCN ATTCGCANCG CATCNCCTTC TATCGCCTNC   901 NTGANNANTT CTTCTGANCG GGNNTCTNGG NNNTNCNAAA TGANNNNNCN AAGCGNNNNC 961 NNANNCNNNN CNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

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