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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pN1-TOMM20-Halo

Halo Tag, Mitochondrial outer membrane localization

Catalog number RDB17750
Resource name pN1-TOMM20-Halo
Alternative name (stock no. 1131)
Clone info. Halo Tag, Mitochondrial outer membrane localization (TOMM20). CMV promoter.
Comment Expression was confirmed by the depositor in mammalian cell.
Vector backbone pN1 (plasmid)
Gene/insert name Rhodococcus rhodochrous dehalogenase genomic DNA human TOMM20 cDNA
Depositor|Developer Okada, Yasushi | Yaginuma, Hideyuki |
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External Database
Rhodococcus rhodochrous dehalogenase
        human TOMM20

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Wang et al, Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Wang et al, Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) 。本件リソースは非営利機関の学術研究目的にのみ利用することができる。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17750 pN1-TOMM20-Halo DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Wang, C., A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae. Proc. Natl. Acad. Sci. U.S.A. 116 (32): 15817-15822 (2019). PMID 31337683.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17750_A9L3p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'(TOMM20,Halo tag 5')
          Sequence file: RDB17750_A9L3a.seq check
          >D06362C2_A9L3_3_CMV_Forward_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNCNNNNNNT NNNNNNNNNT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
             61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGA CTCAGATCTC
            121 GAGCTCAAGC TTCGAATTCG CCACCATGGT GGGTCGGAAC AGCGCCATCG CCGCCGGTGT
            181 ATGCGGGGCC CTTTTCATTG GGTACTGCAT CTACTTCGAC CGCAAAAGAC GAAGTGACCC
            241 CAACTTCAAG AACAGGCTTC GAGAACGAAG AAAGAAACAG AAGCTTGCCA AGGAGAGAGC
            301 TGGGCTTTCC AAGTTACCTG ACCTTAAAGA TGCTGAAGCT GTTCAGAAGT TCTTCCTTGA
            361 AGAAATACAG CTTGGTGAAG AGTTACTAGC TCAAGGTGAA TATGAGAAGG GCGTAGACCA
            421 TCTGACAAAT GCAATTGCTG TGTGTGGACA GCCACAGCAG TTACTGCAGG TCTTACAGCA
            481 AACTCTTCCA CCACCAGTGT TCCAGATGCT TCTGACTAAG CTCCCAACAA TTAGTCAGAG
            541 AATTGTAAGT GCTCAGAGCT TGGCTGAAGA TGATGTGGAA GGCGGTAGCG GGGATCCACC
            601 GGTCTCGACC GGCAGCGAAA TCGGTACTGG CTTTCCATTC GACCCCCATT ATGTGGAAGT
            661 CCTGGGCGAG CGCATGCACT ACGTCGATGT TGGTCCGCGC GATGGCACCC CTGTGCTGTT
            721 CCTGCACGGT AACCCGACCT CCTCCTACGT GTGGNGCAAC ATCATCCCGC ATGTTGCACC
            781 GACCCATCGC TGCATTGCTC CAGACCTGAT CGGTATGGGC AAATCCGACA AACCAGANCT
            841 GGGNTANNNC TTCGACGACC ACGTCCGCTN CATGGATGCN TTCNTCGANN NCCTGGGNCT
            901 NGNAAGANGN CNNCCNNNNN NTTCNCNNNN GGGNNCTNCN NNNTGGGTTT NNNNNNNNNN
          //
          Primer: pAxEF R2
          Region: SV40 pA,insert 3'(Halo tag 3')
          Sequence file: RDB17750_A9L3b.seq check
          >D06362C2_A9L3_3_pAxEF R2_E03_15_ABI24.ab1
              1 NNNNNNNNNN GNTTNNNTTG TGAANTTGTG NNGCTATTGC TTTATTTGTA ACCATTATAA
             61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
            121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
            181 ATCTAGAGTC GCGGCCGCTT AACCGGAAAT CTCCAGAGTA GACAGCCAGC GCGCGATCTC
            241 GCTGCCGATC AGGTCCGGGT TGTCTTCTTG CAGCAGATTC AGACCCGGGC CGATGTCCAC
            301 AGCCTTGCAG TTAGGCAGGC TTTTGGCCAG GCGAGCGGCT TCGGCCGGTG GGATCAGAAC
            361 GCCTGGGGTG CCCCAGAACA GCAGCTTCGG GACAGGGGAC TGGTGCAGCC AGTCCATGTA
            421 TTCTTCGACC AGCGCGACGA TGTTCGCTGG CTCACCGGCG ATTGGCAGCT CGTTTGGGAA
            481 GCGCCACAGT GGCTCGCGGT CAACAGGATT CAGGAACGGC TCGCGGTAAT GGTCCATCTC
            541 GACTTCAGTC AGCGGGCGGA CGACACCCAT CGGCAGCGTA CCCTCGATAA AAACGTTCTG
            601 ATCGATGATC AGCTTGCGGC CGACGTCGGT GGTGCGGAAG GCCTGGAAGG TCTCGCGGGC
            661 AAATTCTGGC CATTCGTCCC AGGTCGGGAT AGGGCGGATG AACTCCATAA ATGCAATACC
            721 TTTGACGCGC TCTGGATTGC GCTTGGCCCA GTGGANNCCC AGANCGGANC CCCAGTCGTG
            781 AATGACCAGG ACGACCTCTT CCAGANCCAN GGCTTCNATG AANNNNTCCA TGAANCGGAN
            841 GTGGTCGNCN NANAAATAAC CCANGTCTGG NTNGNCGNAT TNGCCCNTAC CGATCANGNC
          901 TNGANCNANG CANCGATNGG NNCGGNNNNN CNTGCNGGNN GNTGTNNNNN NNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori, NeoR_KanR
          Sequence file: RDB17750_A9L3c.seq check
          >D06362C2_A9L3_3_SV40pro_ori_F_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNCNNNNN NNNNNNNNGC CTCTGANCTA TTCNNGAAGT AGTGAGGANG
             61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC
            121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
            181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
            241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
            301 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
            361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
            421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
            481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
            541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
            601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
            661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
            721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCANG
            781 ACATAGCGTT GGCTANCCGT GATATTGCTG AAGAGCTTGN CGGCGANTGG GCTGACCGCT
            841 TCCTCGNGCT TTACNGTATC GCCGCTCCCN ATTCGCANCG CATCNCCTTC TATCGCCTNC
            901 NTGANNANTT CTTCTGANCG GGNNTCTNGG NNNTNCNAAA TGANNNNNCN AAGCGNNNNC
          961 NNANNCNNNN CNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Wang, C., A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae. Proc. Natl. Acad. Sci. U.S.A. 116 (32): 15817-15822 (2019). PMID 31337683.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl