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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pN1-OPA1(123Nt)-Halo

Halo Tag, Mitochondrial intermembrane space localization

Catalog number RDB17747
Resource name pN1-OPA1(123Nt)-Halo
Alternative name (stock no. 1128)
Clone info. Halo Tag, Mitochondrial intermembrane space localization (OPA1, 123Nt). CMV promoter.
Comment Expression was confirmed by the depositor in mammalian cell.
Vector backbone pN1 (plasmid)
Gene/insert name Rhodococcus rhodochrous dehalogenase genomic DNA human OPA1 cDNA
Depositor|Developer Okada, Yasushi | Yaginuma, Hideyuki |
Other clones in our bank

External Database
Rhodococcus rhodochrous dehalogenase
        human OPA1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Wang et al, Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) 。本件リソースは非営利機関の学術研究目的にのみ利用することができる。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB17747 pN1-OPA1(123Nt)-Halo DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Wang, C., A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae. Proc. Natl. Acad. Sci. U.S.A. 116 (32): 15817-15822 (2019). PMID 31337683.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17747_A9L3p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'(OPA1(123Nt),Halo tag 5')
          Sequence file: RDB17747_A9L3a.seq check
          >D06362B8_A9L3_1_CMV_Forward_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TNNNNNCAAN GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
             61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CCACCATGTG GCGACTACGT
            121 CGGGCCGCTG TGGCCTGTGA GGTCTGCCAG TCTTTAGTGA AACACAGCTC TGGAATAAAA
            181 GGAAGTTTAC CACTACAAAA ACTACATCTG GTTTCACGAA GCATTTATCA TTCACATCAT
            241 CCTACCTTAA AGCTTCAACG ACCCCAATTA AGGACATCCT TTCAGCAGTT CTCTTCTCTG
            301 ACAAACCTTC CTTTACGTAA ACTGAAATTC TCTCCAATTA AATATGGCTA CCAGCCTCGC
            361 AGGAATTTTT GGCCAGCAAG ATTAGCTACG AGACTCTTAA AACTTCGCTA TCTCATACTA
            421 GGATCGGCTG TTGGGGGTGG CTACACAGCC AAAAAGACTT TTGATCAGTG GAAAGGGGAT
            481 CCACCGGTCT CGACCGGCAG CGAAATCGGT ACTGGCTTTC CATTCGACCC CCATTATGTG
            541 GAAGTCCTGG GCGAGCGCAT GCACTACGTC GATGTTGGTC CGCGCGATGG CACCCCTGTG
            601 CTGTTCCTGC ACGGTAACCC GACCTCCTCC TACGTGTGGC GCAACATCAT CCCGCATGTT
            661 GCACCGACCC ATCGCTGCAT TGCTCCAGAC CTGATCGGTA TGGGCAAATC CGACAAACCA
            721 GACCTGGGTT ATTTCTTCGA CGACCACGTC CGCTTCATGG ATGCCTTCAT CGAAGCCCNG
            781 GGTCTGGAAG ANGTCGTCCT GGTCATTCAC GACTGGGGCT CCGCTCNGGG TTTCCACTGG
            841 GCCNAGCGCA ATCCAGANCG CGTCNANNGT ANNGCATTTA TGGAGTTCAT CCGCCNNATN
            901 CNNANNNNNN NGAANNGNCA GNANTTNNCC NNNNNNNNCT TNCNNNNNNT NCGNNNNNNN
          961 NNN
          //
          Primer: pAxEF R2
          Region: SV40 pA,insert 3'(Halo tag 3')
          Sequence file: RDB17747_A9L3b.seq check
          >D06362B8_A9L3_1_pAxEF R2_B03_06_ABI24.ab1
              1 NNNNNNNNAN NGNNTNATTT GTGAANTTGT GNNGCTATTG CTTTATTTGT AACCATTATA
             61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
            121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
            181 GATCTAGAGT CGCGGCCGCT TAACCGGAAA TCTCCAGAGT AGACAGCCAG CGCGCGATCT
            241 CGCTGCCGAT CAGGTCCGGG TTGTCTTCTT GCAGCAGATT CAGACCCGGG CCGATGTCCA
            301 CAGCCTTGCA GTTAGGCAGG CTTTTGGCCA GGCGAGCGGC TTCGGCCGGT GGGATCAGAA
            361 CGCCTGGGGT GCCCCAGAAC AGCAGCTTCG GGACAGGGGA CTGGTGCAGC CAGTCCATGT
            421 ATTCTTCGAC CAGCGCGACG ATGTTCGCTG GCTCACCGGC GATTGGCAGC TCGTTTGGGA
            481 AGCGCCACAG TGGCTCGCGG TCAACAGGAT TCAGGAACGG CTCGCGGTAA TGGTCCATCT
            541 CGACTTCAGT CAGCGGGCGG ACGACACCCA TCGGCAGCGT ACCCTCGATA AAAACGTTCT
            601 GATCGATGAT CAGCTTGCGG CCGACGTCGG TGGTGCGGAA GGCCTGGAAG GTCTCGCGGG
            661 CAAATTCTGG CCATTCGTCC CAGGTCGGGA TAGGGCGGAT GAACTCCATA AATGCAATAC
            721 CTTTGACGCG CTCTGGATTG CGCTTGGCCC AGTGGAAACC CAGANCGGAN CCCCAGTCGT
            781 GAATGACCAG GACGANCTCT TCCAGANCCA NGGCTTCNAT GAANGCATCC NTGANNCGNA
            841 NGTGGTCGTC NAAGAAATAA CCCANGNCNG GTTTGNCNGA NTTGCCCATA CCNNTCNNGN
            901 CTGGANCAAN GCANCNATGG GNNNGNNCNN NATGCNGGNN NANGTNGCNN CNCNNNNNNN
          961 NNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori, NeoR_KanR
          Sequence file: RDB17747_A9L3c.seq check
          >D06362B8_A9L3_1_SV40pro_ori_F_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNC GCCTCGGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT
             61 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC
            121 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC
            181 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA
            241 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG
            301 CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG
            361 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
            421 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG
            481 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC
            541 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA
            601 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC
            661 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT
            721 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGANCG CTATCAGGAC
            781 ATAGCGTTGG CTACNCGTGA TATTGCTGAA GANCTTGGCG GCGAATGGGC TGANCGCTTC
            841 CTCGNGCTTT ANGGNATCGC CGCTCCCNAT TCNCAGCGCA TCGCCTNCNN TCGCCTNCTT
            901 GNCNANTTCN TCTGANCGGG NNTNNGGGGN NTNNGAAANG ANCNNNNNAN NNNNNNCNNN
          961 NCNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Wang, C., A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae. Proc. Natl. Acad. Sci. U.S.A. 116 (32): 15817-15822 (2019). PMID 31337683.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl