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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pN1-OPA1(123Nt)-Halo (#RDB17747)

Halo Tag, Mitochondrial intermembrane space localization

Alternative name (stock no. 1128)
Clone info. Halo Tag, Mitochondrial intermembrane space localization (OPA1, 123Nt). CMV promoter.
Comment Expression was confirmed by the depositor in mammalian cell.
Vector backbone pN1 (plasmid)
Gene/insert name Rhodococcus rhodochrous dehalogenase genomic DNA human OPA1 cDNA
Depositor|Developer Okada, Yasushi | Yaginuma, Hideyuki |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Wang et al, Wang et al, Proc. Natl. Acad. Sci. U S A, 116(32): 15817-15822, 2019) 。本件リソースは非営利機関の学術研究目的にのみ利用することができる。
MTAに書く付加的使用条件:
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17747 pN1-OPA1(123Nt)-Halo DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pN1-OPA1(123Nt)-Halo was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17747).

Reference section:

Wang, C., Taki, M., Sato, Y., Tamura, Y., Yaginuma, H., Okada, Y., Yamaguchi, S., A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae. Proc. Natl. Acad. Sci. U.S.A. 116 (32): 15817-15822 (2019). PMID 31337683. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17747_A9L3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'(OPA1(123Nt),Halo tag 5')
Sequence file: RDB17747_A9L3a.seq check
>D06362B8_A9L3_1_CMV_Forward_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TNNNNNCAAN GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CCACCATGTG GCGACTACGT
  121 CGGGCCGCTG TGGCCTGTGA GGTCTGCCAG TCTTTAGTGA AACACAGCTC TGGAATAAAA
  181 GGAAGTTTAC CACTACAAAA ACTACATCTG GTTTCACGAA GCATTTATCA TTCACATCAT
  241 CCTACCTTAA AGCTTCAACG ACCCCAATTA AGGACATCCT TTCAGCAGTT CTCTTCTCTG
  301 ACAAACCTTC CTTTACGTAA ACTGAAATTC TCTCCAATTA AATATGGCTA CCAGCCTCGC
  361 AGGAATTTTT GGCCAGCAAG ATTAGCTACG AGACTCTTAA AACTTCGCTA TCTCATACTA
  421 GGATCGGCTG TTGGGGGTGG CTACACAGCC AAAAAGACTT TTGATCAGTG GAAAGGGGAT
  481 CCACCGGTCT CGACCGGCAG CGAAATCGGT ACTGGCTTTC CATTCGACCC CCATTATGTG
  541 GAAGTCCTGG GCGAGCGCAT GCACTACGTC GATGTTGGTC CGCGCGATGG CACCCCTGTG
  601 CTGTTCCTGC ACGGTAACCC GACCTCCTCC TACGTGTGGC GCAACATCAT CCCGCATGTT
  661 GCACCGACCC ATCGCTGCAT TGCTCCAGAC CTGATCGGTA TGGGCAAATC CGACAAACCA
  721 GACCTGGGTT ATTTCTTCGA CGACCACGTC CGCTTCATGG ATGCCTTCAT CGAAGCCCNG
  781 GGTCTGGAAG ANGTCGTCCT GGTCATTCAC GACTGGGGCT CCGCTCNGGG TTTCCACTGG
  841 GCCNAGCGCA ATCCAGANCG CGTCNANNGT ANNGCATTTA TGGAGTTCAT CCGCCNNATN
  901 CNNANNNNNN NGAANNGNCA GNANTTNNCC NNNNNNNNCT TNCNNNNNNT NCGNNNNNNN
961 NNN
//
Primer: pAxEF R2 (Pr0059)
Region: SV40 pA,insert 3'(Halo tag 3')
Sequence file: RDB17747_A9L3b.seq check
>D06362B8_A9L3_1_pAxEF R2_B03_06_ABI24.ab1
    1 NNNNNNNNAN NGNNTNATTT GTGAANTTGT GNNGCTATTG CTTTATTTGT AACCATTATA
   61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
  121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
  181 GATCTAGAGT CGCGGCCGCT TAACCGGAAA TCTCCAGAGT AGACAGCCAG CGCGCGATCT
  241 CGCTGCCGAT CAGGTCCGGG TTGTCTTCTT GCAGCAGATT CAGACCCGGG CCGATGTCCA
  301 CAGCCTTGCA GTTAGGCAGG CTTTTGGCCA GGCGAGCGGC TTCGGCCGGT GGGATCAGAA
  361 CGCCTGGGGT GCCCCAGAAC AGCAGCTTCG GGACAGGGGA CTGGTGCAGC CAGTCCATGT
  421 ATTCTTCGAC CAGCGCGACG ATGTTCGCTG GCTCACCGGC GATTGGCAGC TCGTTTGGGA
  481 AGCGCCACAG TGGCTCGCGG TCAACAGGAT TCAGGAACGG CTCGCGGTAA TGGTCCATCT
  541 CGACTTCAGT CAGCGGGCGG ACGACACCCA TCGGCAGCGT ACCCTCGATA AAAACGTTCT
  601 GATCGATGAT CAGCTTGCGG CCGACGTCGG TGGTGCGGAA GGCCTGGAAG GTCTCGCGGG
  661 CAAATTCTGG CCATTCGTCC CAGGTCGGGA TAGGGCGGAT GAACTCCATA AATGCAATAC
  721 CTTTGACGCG CTCTGGATTG CGCTTGGCCC AGTGGAAACC CAGANCGGAN CCCCAGTCGT
  781 GAATGACCAG GACGANCTCT TCCAGANCCA NGGCTTCNAT GAANGCATCC NTGANNCGNA
  841 NGTGGTCGTC NAAGAAATAA CCCANGNCNG GTTTGNCNGA NTTGCCCATA CCNNTCNNGN
  901 CTGGANCAAN GCANCNATGG GNNNGNNCNN NATGCNGGNN NANGTNGCNN CNCNNNNNNN
961 NNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB17747_A9L3c.seq check
>D06362B8_A9L3_1_SV40pro_ori_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC GCCTCGGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC
  121 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC
  181 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA
  241 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG
  301 CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG
  361 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
  421 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG
  481 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC
  541 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA
  601 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC
  661 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT
  721 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGANCG CTATCAGGAC
  781 ATAGCGTTGG CTACNCGTGA TATTGCTGAA GANCTTGGCG GCGAATGGGC TGANCGCTTC
  841 CTCGNGCTTT ANGGNATCGC CGCTCCCNAT TCNCAGCGCA TCGCCTNCNN TCGCCTNCTT
  901 GNCNANTTCN TCTGANCGGG NNTNNGGGGN NTNNGAAANG ANCNNNNNAN NNNNNNCNNN
961 NCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Wang, C., A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae. Proc. Natl. Acad. Sci. U.S.A. 116 (32): 15817-15822 (2019). PMID 31337683. [link to RRC of NBRP]