Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEBMulti-Neo-human-eIF2beta (#RDB17726)

Expression vector of human eukaryotic translation initiation factor 2 subunit beta (eIF2beta).

Alternative name eIF2beta
Clone info. Expression vector of human eukaryotic translation initiation factor 2 subunit beta (eIF2beta) cloned into the pEBMulti-Neo.
Comment Expression was confirmed by the depositor.
Vector backbone pEBMulti-Neo (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells).
Gene/insert name human EIF2S2 cDNA
Depositor|Developer Ito, Takuhiro |
 
Sequence (partial) RDB17726pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17726 pEBMulti-Neo-human-eIF2beta DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEBMulti-Neo-human-eIF2beta was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17726).

Reference section:

Kashiwagi, K., Yokoyama, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Shirouzu, M., Ito, T., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17726_A9Kcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584)
Region: CAG pro, insert 5'
Sequence file: RDB17726_A9Kca.seq check
>D06721A5_B0Ju_1_CAG_R1_A09_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCNNGCGG GCGCGGGGCG AANCGGTGCG GCGCCGGCAG
   61 GAAGGAAATG GGCGGGGNNG GCCTTCGTGC GTCGCCGCGC CGCCGTCCCC TTCTCCCTCT
  121 CCAGCCTCGG GGCTGTCCGC GGGGGGACGG CTGCCTTCGG GGGGGACGGG GCAGGGCGGG
  181 GTTCGGCTTC TGGCGTGTGA CCGGCGGCTC TAGAGCCTCT GCTAACCATG TTCATGCCTT
  241 CTTCTTTTTC CTACAGCTCC TGGGCAACGT GCTGGTTATT GTGCTGTCTC ATCATTTTGG
  301 CAAAGAATTA ACCCTCACTA AAGGGGTACC GGGCCCCCCC TCGAGGTCGA CGCCACCATG
  361 TCTGGGGACG AGATGATTTT TGATCCTACT ATGAGCAAGA AGAAAAAGAA GAAGAAGAAG
  421 CCTTTTATGT TAGATGAGGA AGGGGATACC CAAACAGAGG AAACCCAGCC TTCAGAAACA
  481 AAAGAAGTGG AGCCAGAGCC AACTGAGGAC AAGGATTTGG AAGCTGATGA AGAGGACACT
  541 AGGAAAAAAG ATGCTTCTGA TGATCTAGAT GACTTGAACT TCTTTAATCA AAAGAAAAAG
  601 AAGAAAAAAA CTAAAAAGAT ATTTGATATT GATGAAGCTG AAGAAGGTGT AAAGGATCTT
  661 AAGATTGAAA GTGATGTTCA AGAACCAACT GAACCAGAGG ATGACCTTGA CATTATGCTT
  721 GGCAATAAAA AGAAGAAAAA GAAGAATGTT AAGTTCCCAG ATGANGATGA AATACTAGAG
  781 AAAGANGANG CTCTAGAAGA TGAAGANAAC AAAAAAGANG ATGGTATCTC NTTCAGTAAT
  841 CAGACAGGCC CNGCTTGGGC AGGCTCNNAA AGAGACTACA CATACNANGA GCTGCNNAAT
  901 CGAGNGNNCA NCATCATGAN GGAAAAGAAN NCNNNATNTN GNNNNCTGGG GANAAAANNA
  961 AANTTGTCAN GAAANCNTNN NNNNNNCNNN NNNNANNNNN ANNNNAGAAA NNNTNNNNNN
1021 NNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: SV40 pA, insert 3'
Sequence file: RDB17726_A9Kcb.seq check
>D06721A5_A9Kc_1_SV40polyA-R2_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNANNNNNNN TAANAANNAC AATTGCATTC NTTTTATGTT
   61 TCAGGTTCNG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG
  121 TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTAGTTAG CTTTGGCACG GAGCTGTGCT
  181 CGCTTGCCCG TGACAGCCTG GAAGCCGGTT TTGATACTGG CAACAGAACA TCTAGAATGA
  241 CAAGTTTCGC ACTGTAGGAA ATAGAGTCGT GTGTCCTTCT GCAGGATTGT GTCCGGTGAT
  301 CGGCATGTGT GACAAGTGAC ATATTCCTTG ATATATCTTC TCAAGACATT TTCTATCTGT
  361 TTCTGTTGGA ATCTTCCTTT GATTACAAGT TGGTTATTAC CATCTATAGA ACCACTTGTA
  421 CCCAATTCAG CCAACAAAAA TGCAAGGAGA TGTTTGGGCT GACGATGTAA TAGTTTACAG
  481 ATATCTGTAA AGTTGACAAA AGAAGTTTTC TTGGTTCCTA CTCGGACGAC TTGTGGAGGT
  541 TTCATGACAA ATTTCCTTTT CTCCCCAGCA ACCATATCTG GATTCTTTTC CCTCATGATG
  601 TTGAACACTC GATTCAGCAG CTCCTCGTAT GTGTAGTCTC TTTCTGAGCC TGCCCAAGCA
  661 GGGCCTGTCT GATTACTGAA TGAGATACCA TCATCTTTTT TGTTGTCTTC ATCTTCTAGA
  721 GCTTCATCTT TCTCTAGTAT TTCATCCTCA TCTGGGAACT TAACATTCTT CTTTTTCTTC
  781 TTTTTATTGC CAAGCATAAT GTCAAGGTCA TCCTCTGGTT CAGTTGGNTC TTGAACATCA
  841 CTTTCAATCT TAAGATCCNT TACACCTTCT TCAGCNNCNT CAATATCAAA TATCTTTTTA
  901 GTTTTTTTTC NTCTTTTNNT TTTGANTNAA NAANTTCANG TCNTCTAGAT CNNNNNNNNN
961 N
//
Primer: oriP_F (Pr0765)
Region: oriP, EBNA-1
Sequence file: RDB17726_A9Kcc.seq check
>D06721A5_A9Kc_1_oriP_F_G04_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG GGCGTNNNTT TCGCTGCTTG TCCTTTTCCT
   61 GCTGGTTGCT CCCATTCTTA GGTGAATTTA AGGAGGCCAG GCTAAAGCCG TCGCATGTCT
  121 GATTGCTCAC CAGGTAAATG TCGCTAATGT TTTCCAACGC GAGAAGGTGT TGAGCGCGGA
  181 GCTGAGTGAC GTGACAACAT GGGTATGCCC AATTGCCCCA TGTTGGGAGG ACGAAAATGG
  241 TGACAAGACA GATGGCCAGA AATACACCAA CAGCACGCAT GATGTCTACT GGGGATTTAT
  301 TCTTTAGTGC GGGGGAATAC ACGGCTTTTA ATACGATTGA GGGCGTCTCC TAACAAGTTA
  361 CATCACTCCT GCCCTTCCTC ACCCTCATCT CCATCACCTC CTTCATCTCC GTCATCTCCG
  421 TCATCACCCT CCGCGGCAGC CCCTTCCACC ATAGGTGGAA ACCAGGGAGG CAAATCTACT
  481 CCATCGTCAA AGCTGCACAC AGTCACCCTG ATATTGCAGG TAGGAGCGGG CTTTGTCATA
  541 ACAAGGTCCT TAATCGCATC CTTCAAAACC TCAGCAAATA TATGAGTTTG TAAAAAGACC
  601 ATGAAATAAC AGACAATGGA CTCCCTTAGC GGGCCAGGTT GTGGGCCGGG TCCAGGGGCC
  661 ATTCCAAAGG GGAGACGACT CAATGGTGTA AGACGACATT GTGGAATAGC AAGGGCAGTT
  721 CCTCGCCTTA NGTTGTAAAG GGAGGTCTTA CTACCTCCAT ATACGAACAC ACCGGCGANC
  781 CAAGTTCCTT CGTCGGTAGT CCTTTCTACG TGACTCCTAN CCNGGAGAGC TCTTANACCT
  841 TCTGCAATGN TCTCAAATTT CNGGTTGNAA CCTCCNTGAN CACNATGCTT TCCNAACCAC
  901 CCTCCTTTTT TGCGCCTGNC TNCNTCACCN NGACCCCGGG GNNNAGNGNT TGGGNCTTCT
  961 CCTNNNCATC TGNGGNNNCT GNTCTATNGN TNCNNNGGGN NNGTNNNGNN NNNCNNCNGG
1021 GNNNNNNTNN NNNNNGTANN NNAAANNNNN NNNNTNNNNN NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_Kan_R
Sequence file: RDB17726_A9Kcd.seq check
>D06721A5_A9Kc_1_SV40pro_ori_F_F04_16_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNNNN NNNNNCTCGG CCTCTGAGCT ATTCCNGAAG TAGTGAGGAG
   61 GCTTTTTTGG ANGCCTAGGC TTTTGCAAAG ATCATCAAGA GACAGGATGA GGATCGTTTC
  121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
  181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
  241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
  301 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
  361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
  421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
  481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
  541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
  601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
  661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
  721 ATGGNCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCAGG
  781 ACATAGCGTT GGCTACCCGT GATATTGNTG AANAGCTTGG CGGCGAATGG GCTGACCGCT
  841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCNCCTTC TATCNNCTNC
  901 TTGACGAGTN CTTCTGANCG GGANTCTNGN NNTCNAAATG ACCGANCAAG CGACNCCCNA
  961 NCNGNCATCN CNAGATTTCN ATNCCNNNGN CNNNNTNNNA TNNNNNNNNG GCNTNCNGNN
1021 NNNNTTTNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]