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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pENTR hRFWD3 (#RDB17502)

Gateway(R) Entry vector of human ring finger and WD repeat domain 3 (RFWD3).

Clone info. Gateway(R) Entry vector of human ring finger and WD repeat domain 3 (RFWD3).
Comment Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone pENTR (plasmid)
Selectable markers Kanamycin
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
備考
Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17502 pENTR hRFWD3 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pENTR hRFWD3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17502).

Reference section:

Inano, S., Sato, K., Katsuki, Y., Kobayashi, W., Tanaka, H., Nakajima, K., Nakada, S., Miyoshi, H., Knies, K., Takaori-Kondo, A., Schindler, D., Ishiai, M., Kurumizaka, H., Takata, M., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17502_A9Iop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: rrnBT1ter_F2 (Pr0531)
Region: rrnB T1 ter, attL1, insert 5'
Sequence file: RDB17502_A9Ioa.seq check
>D06551B4_A9Io_2_rrnBT1ter_F2_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCCTTTCGT TTNNTTTGAT GCCTGGCAGT TCCCTACTCT
   61 CGCGTTAACG CTAGCATGGA TGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTCT
  121 TAAGCTCGGG CCCCAAATAA TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA
  181 AATTGATGAG CAATGCTTTT TTATAATGCC AACTTTGTAC AAAAAAGCAG GCTCCGCGGC
  241 CGCCCCCTTC ACCATGGCTC ATGAAGCAAT GGAATATGAT GTTCAGGTGC AGTTAAATCA
  301 TGCCGAACAA CAGCCAGCTC CTGCTGGCAT GGCCAGCAGC CAAGGGGGAC CAGCCCTCCT
  361 CCAGCCTGTT CCTGCTGATG TGGTCAGCAG CCAGGGGGTA CCATCCATCC TCCAGCCAGC
  421 TCCTGCTGAG GTGATCAGCA GCCAAGCGAC ACCACCCCTG CTCCAGCCTG CTCCGCAACT
  481 GTCTGTTGAC CTGACAGAAG TGGAGGTCTT GGGAGAAGAC ACTGTGGAGA ACATCAATCC
  541 AAGAACTTCA GAACAACATA GGCAGGGATC TGATGGTAAT CACACCATCC CAGCATCTTC
  601 GTTGCATTCA ATGACCAACT TCATCAGCGG ACTGCAGAGA CTTCATGGCA TGCTGGAATT
  661 CCTGAGACCT TCATCTTCAA ACCACAGTGT AGGGCCAATG AGAACAAGAA GGAGGGTATC
  721 TGCTTCACGG AGGGCAAGAG CCGGANGGTC TCAGANGACA GACAGTGCCA GGTTGAGAGC
  781 ACCATTGGAT GCTTACTTTC AGGTGAGCAG GANCCAGCCT GACTTGCCAG CTACCACTTA
  841 TGATTCAGAN ACTANGANTC CTGTATCTGA AGANTTGCAN GTGTCTAGTA NTTCTGATTC
  901 TGACAGTGAC AGCTCTGCAG ANTATGGANG GGGNTGTTGA NNNNNCANAN GNANNTGGAN
961 CTNNCATTTT NANAANNANC NACNANNNNN NNGTCTCANC ANNNNNNNNN N
//
Primer: KanR-N_R (Pr0530)
Region: attL2, insert 3'
Sequence file: RDB17502_A9Iob.seq check
>D06551B4_A9Io_2_KanR-N_R_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNAGCATC NNTGTTGNNN NNTAATCGCG GCCTCGACGT
   61 TTCCCGTTGA NNNNNNNNNN NNNNACCCCT TGTATTACTG TTTATGTAAG CAGACAGTTT
  121 TATTGTTCAT GATGATATAT TTTTATCTTG TGCAATGTAA CATCAGAGAT TTTGAGACAC
  181 GGGCCAGAGC TGCCAGGAAA CAGCTATGAC CATGTAATAC GACTCACTAT AGGGGATATC
  241 AGCTGGATGG CAAATAATGA TTTTATTTTG ACTGATAGTG ACCTGTTCGT TGCAACAAAT
  301 TGATAAGCAA TGCTTTCTTA TAATGCCAAC TTTGTACAAG AAAGCTGGGT CGGCGCGCCC
  361 ACCCTTCTCC CACTTATAGA TGTGGACCAT CTTCTCTGTT AAGGTAGCCA AGTAGCTGTT
  421 ACGGTTCACC TCAAATGGGC AGATGTCCAA CACAGGCTGA TCGGTCTGTA GGTCCTGGAG
  481 CAACGAGCCA CTGGCAGCAT CCCACAGCAG GGCAGAATTT GCTGCTTCAT CCCCAGTNNN
  541 NNNCAGGATG TTGCCATCAT TCTCTGGGCT TTGGAAAATG GCATTTTTGG NCNATAGTTT
  601 GCNAGTAGGT CCTCCAAAAA ATGTATGTAC AGGCTGGCAN GAGCAGATTG NANTTCCAGN
  661 GTCNTCCAGT CNGTAGGACA TTTNCATCAG NNCACTTCGN ATNGNNGNNG TGNNTTTTNN
721 CNNNNNNNNN N
//
Primer: hRFWD3-N_F (Pr0753)
Region: insert mid
Sequence file: RDB17502_A9Ioc.seq check
>D06551B4_A9Io_2_hRFWD3-N_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTGANN ANNTNCAGGT GTCTAGTAGT TCTGANTCTG
   61 ACANNNNNNN NCTCTGCAGA GTATGGAGGG GTTGTTGACC AGGCAGANGA ATCTGGAGCT
  121 GTCATTTTAG AAGAGCAACT AGCAGGTGTC TCAGCAGAGC AAGAAGTTAC ATGTATCGAT
  181 GGAGGCAAGA CCCTCCCCAA ACAGCCATCT CCCCAGAAGT CTGAGCCTCT GCTACCTTCT
  241 GCTTCTATGG ATGAGGAAGA AGGGGACACT TGTACAATAT GTCTGGAACA GTGGACCAAT
  301 GCTGGGGACC ACCGGCTCTC AGCATTACGC TGTGGGCATC TCTTTGGGTA TAGGTGCATT
  361 TCCACGTGGC TTAAAGGACA AGTACGAAAA TGTCCCCAGT GCAACAAGAA AGCCAGGCAC
  421 AGTGACATTG TCGTCCTTTA TGCCCGAACC CTGAGAGCTT TGGACACTAG TGAACAGGAG
  481 CGCATGAAAA GTTCCCTACT GAAGGAACAG ATGCTAAGGA AACAGGCCGA GTTAGAATCA
  541 GCACAGTGCC GACTCCAACT GCAGGTCCTC ACTGATAAGT GCACTAAGCT TCAAAGGCGT
  601 GTTCAGGACT TGNAAAAACT TACGTCACAT CAAAGTCAGA ATTTACAGCA ACCCAGGGGC
  661 TCCCAAGCAT GGGTCCTGAG CTGCTCACCC TCCAGCCAGG GCCAGCACAA GCNCAAGTAC
  721 CACTTCCAAA AGANCTTCAC AGTATCTCAG GCAGGAAACT GCCGGATCAT GGCATACTGT
  781 GATGCTCTGA GCTGCCTGGT GATATCACAG CCNNCTCCTC NNNNCTCTTT TCTNCCANGC
  841 TTTGGTGTTN AGATGTTGAG TACTGCCNAC ATGANNAGCA GTCAGTACAT TNCGATGCNT
  901 GGGCAANNAG ATNCGNNGNA CNNNNNTTTA NCNNTNACNT CNNANGCTTG CNANTNNCTG
961 NTNNNNNANN NNNCANNATN AAACNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]