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CSII FLAG-hRFWD3 C315A/I639K (#RDB17500)

Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, C315A/I639K (C315A, RING finger domain mutation. I639K, chromatin localization-defective mutation).

Clone info. Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, C315A/I639K (C315A, RING finger domain mutation. I639K, chromatin localization-defective mutation), tagged with FLAG at N-terminus, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone CSII-CMV-MCS-IRES-Bsd (RDB04385) (plasmid)
Selectable markers Lentivirus transfection: Bsd^r. E. coli: Amp^r. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions LB+Ampicillin, 37oC
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
付加的使用条件：
本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17500 CSII FLAG-hRFWD3 C315A/I639K DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII FLAG-hRFWD3 C315A/I639K was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17500).

Reference section:

Inano, S., Sato, K., Katsuki, Y., Kobayashi, W., Tanaka, H., Nakajima, K., Nakada, S., Miyoshi, H., Knies, K., Takaori-Kondo, A., Schindler, D., Ishiai, M., Kurumizaka, H., Takata, M., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17500_A9Iop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: hRFWD3-N_R (Pr0757)
Region: insert 5', attB1, FLAG, CMV pro 2, CMV en 2
Sequence file: RDB17500_A9Ioa.seq check
>D06551B2_A9Io_1_hRFWD3-N_R_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNN TNNGCANGNN CTGGCTGGAN GNNGGATGGT
   61 ACCCCCTGGC TGCTGACCAC ATCAGCAGGA ACAGGCTGGA GGAGGGCTGG TCCCCCTTGG
  121 CTGCTGGCCA TGCCAGCAGG AGCTGGCTGT TGTTCGGCAT GATTTAACTG CACCTGAACA
  181 TCATATTCCA TTGCTTCATG AGCCATGGTG AAGGGGGCGG CCGCGGAGCC TGCTTTTTTG
  241 TACAAACTTG TTTGATAGCT TGGACCAGAA CCACCATGCT TATCGTCATC ATCTTTATAA
  301 TCCATGGTGC TAGCCAGCTT GGGTCTCCCT AGCGGATCTG ACGGTTCACT AAACCAGCTC
  361 TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT
  421 TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT
  481 CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT
  541 ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG
  601 TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT
  661 TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG
  721 TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG
  781 GAACATACGT CATTATTGAC GTCNATGGGC GGGGGTCGTT GNGCGGTCAG CCAGGCGGGC
  841 CATTTACCGT AAGTTATGTA ACGCGGANNT CCNTATATGG NCNATGAACT AATGNNCCCG
  901 TAATTGATTA CTATTAATCG AANTAANTCN AAGTGAANTT CNNNNNNCCN NNTANTAAAC
  961 CCNAAAATTT TGAATTTTNG NANNTTGNTT TTGNANTTNN TNANNTNNNN NNNNNGNTNN
1021 NNNNNANNNN NNNNNN
//
Primer: IRES_R3 (Pr0573)
Region: attB2, insert 3' (I639K)
Sequence file: RDB17500_A9Iob.seq check
>D06551B2_A9Io_1_IRES_R3_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNGNNNNNNG NNGGNNNNGG GNGGNNCCGC GGCCGCTGCA GAATTCTCGA
   61 TGGCAACTAG AAGGCACAGT CGAGGCTGAT CAGCGGGTTT ATCGAACCAC TTTGTACAAG
  121 AAAGCTGGGT CGGCGCGCCC ACCCTTTCAC TCCCACTTAT AGATGTGGAC CATCTTCTCT
  181 GTTAAGGTAG CCAAGTAGCT GTTACGGTTC ACCTCAAATG GGCAGATGTC CAACACAGGC
  241 TGATCGGTCT GTAGGTCCTG GAGCAACGAG CCACTGGCAG CATCCCACAG CAGGGCAGAA
  301 TTTGCTGCTT CATCCCCAGT ACACACCAGG ATGTTGCCAT CATTCTCTGG GCTTTGGAAA
  361 ATGGCATTTT TGGTCAATAG TTTGCAAGTA GGTCCTCCAA AAAATGTATG TACAGGCTGG
  421 CAGGAGCAGA TTGGATTTCC AGTGTCATCC AGTCGGTAGG ACATTTCCAT CAGCACACTT
  481 CGTATGGTGG TGTGATTTTT ATCAGGCCTG TAGGTCACAA GACAGTGCCG GGAGCTGTTC
  541 TCTGTCTGAA AGTCTTTGCA CCCCCCTGGC TCCAAGGGCA GCACATGAGG CCAATGAGAA
  601 AAGTCCATTT TCTGTTCCCA GAATGAAGCA TCCTCCAAGG TTCCAGCCAG CACCCCACCA
  661 TATGGAAATG CAGCTGAGGC AGCTCTGGGC ATGTATGACA GGGAGACCAG TGGGCATCTG
  721 GCTTTCTGAG CTACTAACTC CTGCACATGA CTGCTCGTGT TTCGCACGTC ATATACCAGA
  781 ATTGAACCAT TGGCCAGTCC AGCATAGATG TAGTTAGCCT CATCAAGACA CCAGCAACAG
  841 CTCCAGACAG GACGTCCAGC ATTATAAGTC TGGACCACGG NATNNGTCTC CAGGCTGGNC
  901 AGTTNAATAG NGTTGTCNAN GGAANCAGAN ANTANCAAGC NNCTNANGNN NNNGCNNAAC
961 GCCAGNNCNC GNNNCTNNTT GNNNTGCATN NNNNNNNNN
//
Primer: hRFWD3-N_F (Pr0753)
Region: insert mid (C315A)
Sequence file: RDB17500_A9Ioc.seq check
>D06551B2_A9Io_1_hRFWD3-N_F_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTCTGANNN TTGCAGGTGT CTAGTAGTTC TGATTCTGAC
   61 AGTGACAGCT CTGCAGAGTA TGGAGGGGTT GTTGACCAGG CAGAGGAATC TGGAGCTGTC
  121 ATTTTAGAAG AGCAACTAGC AGGTGTCTCA GCAGAGCAAG AAGTTACATG TATCGATGGA
  181 GGCAAGACCC TCCCCAAACA GCCATCTCCC CAGAAGTCTG AGCCTCTGCT ACCTTCTGCT
  241 TCTATGGATG AGGAAGAAGG GGACACTTGT ACAATATGTC TGGAACAGTG GACCAATGCT
  301 GGGGACCACC GGCTCTCAGC ATTACGCTGT GGGCATCTCT TTGGGTATAG GGCCATTTCC
  361 ACGTGGCTTA AAGGACAAGT ACGAAAATGT CCCCAGTGCA ACAAGAAAGC CAGGCACAGT
  421 GACATTGTCG TCCTTTATGC CCGAACCCTG AGAGCTTTGG ACACTAGTGA ACAGGAGCGC
  481 ATGAAAAGTT CCCTACTGAA GGAACAGATG CTAAGGAAAC AGGCCGAGTT AGAATCAGCA
  541 CAGTGCCGAC TCCAACTGCA GGTCCTCACT GATAAGTGCA CTAAGCTTCA AAGGCGTGTT
  601 CAGGACTTGC AAAAACTTAC GTCACATCAA AGTCAGAATT TACAGCAACC CAGGGGCTCC
  661 CAAGCATGGG TCCTGAGCTG CTCACCCTCC AGCCAGGGCC AGCACAAGCA CAAGTACCAC
  721 TTCCAAAAGA CCTTCACAGT ATCTCAGGCA GGAAACTGCC GGATCATGGC ATACTGTGAT
  781 GCTCTGANCT GCCTGGTGAT ATCACAGCCT TCTCNNCAGG CCTCTTTTCT TCCANGCTTT
  841 NGTGTTAAGA TGTTGAGTAC NGNCNNCATG AAGAGCAGTC AGTACATTCC GATGCATGGC
  901 AAACANATCC GNNNNNTGNC GTTTANCNNT TANNTCNNAN GNTTGCTACT CTCTGNTNNC
961 CNANNNNNNN NNATTAAACN NANNNNCNNN NNNNNNN
//
Primer: HIVgp_R1 (Pr0132)
Region: HIV-1 psi, 5' LTR
Sequence file: RDB17500_A9Iod.seq check
>D06551B2_A9Io_1_HIVgp_R1_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CTCTTTTTTC TCTCTGCNCC NCTCTTCTCT TTGCCTTGGT
   61 GGGTGCTACT CCTAATGGTT CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA
  121 ATTGTCCCTC ATATCTCCTC CTCCAGGTCT GAAGATCAGC GGCCGGCCGC TTGCTGTGCG
  181 GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT
  241 ATCTCTATCC TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA TGATCTAAGT
  301 TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT
  361 GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT
  421 ATATGTTTTA ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC
  481 GATCTAATTC TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT
  541 CCGCTAGTCA AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC
  601 GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG
  661 TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC
  721 TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC
  781 TTTATTGAGG CTTAAGCAGT GGGTTCCCTA NTTAGCCAGA NAGCTCCCAG GCTCANATCT
  841 GGTCTAACCA GAGAGANNNA GTACAGGCAA AACGCGCTGC TTATATAGAC CTCCCACCGN
  901 NCACGCCTAC CGNCCATTTG CGTCNANGGG GGCGGANTTG TNACGANNTT TTGGAAAGTC
  961 CCGTTGNTTT TGGTGCCAAA NNAACNNCCN TNGACNTCNN NGGGGNNNNN NNNTTNGNNN
1021 NNCCNNNNNN NCNNNNCNCN NNNNNNNNCN NNNNNNNNN
//
Primer: cPPT+CTS_v2_F (Pr0303)
Region: cPPT_CTS, CMV en 2, CMV pro2
Sequence file: RDB17500_A9Ioe.seq check
>D06551B2_A9Io_1_cPPT+CTS_v2_F_C01_03_ABI08.ab1
    1 NNNNNNNNNC NNNNNNNNNN NNNTNNNNNN NNNNATTACN AAAATTCAAA ATTTTCGGGT
   61 TTATTACAGG GACAGCAGAA ATTCACTTTG AATTAATTCG ATTAATAGTA ATCAATTACG
  121 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC
  181 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC
  241 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT
  301 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
  361 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT
  421 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC
  481 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC
  541 GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC
  601 TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACNGT GGGAGGTCTA TATAAGCAGA
  661 GCTGGTTTAG TGAACCGNNN NATCNGCTAG GGAGANCCAN NCTGGCTAGC ACCNTGGANT
  721 ATAAAGATGA TGACGATAAG CATGGTGGTT CTGGTCCAAG CTATCNNACA AGTTTGTACA
  781 AAAAAGCANG CTCCGCNGNC GCCCCCTTCA CCNTGGCTCA TGAAGCAATG GAATATGANG
  841 TTCANNTGCN GTTAAATCAT GCCNNANNAA CAGCCAGCTC NNGNNNNNNN TGGCCNNNNN
  901 NNNANGGGGA NNNANCCNNN CNTNCNNNNT GTNNCTGCTG ATGNNNNNAG CNNNCNAGGG
961 GNNNNNNNNN N
//
Primer: IRES-F (Pr0036)
Region: IRES2, BSD, WPRE
Sequence file: RDB17500_A9Iof.seq check
>D06551B2_A9Io_1_IRES-F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCGNNGN NNNNNNNNNT CTAGGCCCCC CGAACCNCGG
   61 GGNNGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GCCAAGCCTT
  121 TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC TACAATCAAC AGCATCCCCA
  181 TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG CGACGGCCGC ATCTTCACTG
  241 GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGTGCAGA ACTCGTGGTG CTGGGCACTG
  301 CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC GATCGGAAAT GAGAACAGGG
  361 GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTGCTTCT CGATCTGCAT CCTGGGATCA
  421 AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT TGGGATTCGT GAATTGCTGC
  481 CCTCTGGTTA TGTGTGGGAG GGCTAAGTCG AGCCTCTAGA GTTAACATCG AGGGATCAAG
  541 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC
  601 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT
  661 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGNTTGCT GTCTCTTTAT
  721 GANGAGTTGT GGNCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA
  781 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCNGGAN TTTCGCTTTC
  841 CCCNNCCCTA TTGNCACGGC GGAANTCATC GCCGNCNGNC TTGCCCGCTG CTGGANANGG
  901 GCTCNNNTGT NNGGNACTGA NNATTCNNNN GNNGTTGNCN GGGAANCTGA CNNNCNTTNN
961 NNNNGCNGNT CNNCNNGNNN TNGNNNNNNN NNN
//
Primer: f1ori_R3 (Pr0142)
Region: bGH p(A), 3' LTR
Sequence file: RDB17500_A9Iog.seq check
>D06551B2_A9Io_1_f1ori_R3_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNN TCTTTCNGCC TCNGAANCCN TAGAGCCCAC
   61 CGCATCCCCA GCATGCCTGC TNNNNNNTTC CCAATCCTCC CCCTTGCTGT CCTGCCCCAC
  121 CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT CCTCATTTTA
  181 TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG GGGAGGGGCA
  241 AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGGG TTTAAACGGG
  301 CCCTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG TTACCAGAGT
  361 CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT TGAGGCTTAA
  421 GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT AACCAGAGAG
  481 ACCCAGTACA GTCCGGATGC AGCTCTCGGG CCATGTGATG AAATGCTAGG CGGCTGTCAA
  541 ACCTCCACTC TAATACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC ACAGGGTGTA
  601 ACAAGCGGGT GTTCTCTCCT TCATTGGCTT CTTCTACCTT CTCTTGCTCA ACTGGTACTA
  661 GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGATCCC TGGCCCTGGT GTGTAGTTCT
  721 GCCAATCAGG GAAGTANNCT TGTGTGTGGT AGATCCACAG ATCAAGGATA TCTTGTCTTC
  781 GTTGGGAGTG AANTNNNNCT TCCNGTCCCC CCTTTTCTTT TAAAAAGTGG CTAAGATCTA
  841 CAGCTGCCTT GTAAGTCATT GGTCTNNAAN GGTACCTGAN GTGTGANTGG NNAAAANCCC
  901 ACCTCCTCCT CCTCNTNGTG CTTNNTAGCC NGGCACNATC AGNNNTTGGN ANCTGCTGNA
  961 NNGNNNCTTG TGATTGCTCC NNGNTTNTTC NNNGNNNNNA CNGNANNGAT GNNNGGNNNN
1021 NNGNCNNNNN GNNNANNCGN NNCNNNTGNA GGNCNNANNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, EM7 pro, BleoR, SV40 p(A)
Sequence file: RDB17500_A9Ioh.seq check
>D06551B2_A9Io_1_SV40pro_ori_F_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCTC TGCCTCTGNN CTATTCNNGA AGTAGTGAGG
   61 AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT
  121 CGGATCTGAT CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA
  181 TACGACAAGG TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC
  241 GCGCGCGACG TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC
  301 TTCGTGGAGG ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG
  361 GTCCAGGACC AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC
  421 GAGCTGTACG CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG
  481 GCCATGACCG AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC
  541 GGCAACTGCG TGCACTTCGT GGCCGAGGAG CAGGACTGAC ACGTGCTACG AGATTTCGAT
  601 TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG
  661 ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT
  721 GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACNA ATTTCACAAA TAAAGCATTT
  781 TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT
  841 ATACCGTCGA NNTCTANCTA GAGCTTGGNC GTANTCATGG TCNTAGCTGT TNCCTGTGTG
  901 AAATTGNTNN TCCGCTCACA ATTNCNCACA ANATACGANC CNNGNANNCN TAAAGNGTAA
  961 AGNCNGGGGG TGNCTAANNN NTGAGCTNNN TCNNNNTNNN NNGCGTTGCG CNNNNNGNCC
1021 NGCTTTNNCN NNNNGGNAAA NCNNNNCGNN NNNNNNCNNC NTTNANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]