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CSII FLAG-hRFWD3 C315A

Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, C315A, RING finger domain mutation.

Catalog number RDB17497
Resource name CSII FLAG-hRFWD3 C315A
Clone info. Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, C315A, RING finger domain mutation, tagged with FLAG at N-terminus, CMV promoter.
Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone CSII-CMV-MCS-IRES-Bsd (plasmid)
Selectable markers Lentivirus transfection: Bsd^r. E. coli: Amp^r. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 
Other clones in our bank human RFWD3 (NCBI Gene 55159) |
Lentiviral plasmid list, maps and sequences information. [link]

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Additional terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering Please visit Ordering instruction.[link] 
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Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17497 CSII FLAG-hRFWD3 C315A DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17497_A9Iop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: hRFWD3-N_R
Region: insert 5', attB1, FLAG, CMV pro2, CMV en
Sequence file: RDB17497_A9Ioa.seq check
>D06551A8_A9Io_3_hRFWD3-N_R_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC CTNNGNAGGN NCTGGCTGGA NGNNGGANGG
   61 TACCCCCTGG CTGCTGACCA CATCAGCAGG AACAGGCTGG AGGAGGGCTG GTCCCCCTTG
  121 GCTGCTGGCC ATGCCAGCAG GAGCTGGCTG TTGTTCGGCA TGATTTAACT GCACCTGAAC
  181 ATCATATTCC ATTGCTTCAT GAGCCATGGT GAAGGGGGCG GCCGCGGAGC CTGCTTTTTT
  241 GTACAAACTT GTTTGATAGC TTGGACCAGA ACCACCATGC TTATCGTCAT CATCTTTATA
  301 ATCCATGGTG CTAGCCAGCT TGGGTCTCCC TAGCGGATCT GACGGTTCAC TAAACCAGCT
  361 CTGCTTATAT AGACCTCCCA CCGTACACGC CTACCGCCCA TTTGCGTCAA TGGGGCGGAG
  421 TTGTTACGAC ATTTTGGAAA GTCCCGTTGA TTTTGGTGCC AAAACAAACT CCCATTGACG
  481 TCAATGGGGT GGAGACTTGG AAATCCCCGT GAGTCAAACC GCTATCCACG CCCATTGATG
  541 TACTGCCAAA ACCGCATCAC CATGGTAATA GCGATGACTA ATACGTAGAT GTACTGCCAA
  601 GTAGGAAAGT CCCATAAGGT CATGTACTGG GCATAATGCC AGGCGGGCCA TTTACCGTCA
  661 TTGACGTCAA TAGGGGGCGT ACTTGGCATA TGATACACTT GATGTACTGC CAAGTGGGCA
  721 GTTTACCGTA AATACTCCAC CCATTGACGT CAATGGAAAG TCCCTATTGG CGTTACTATG
  781 GGAACATACG TCATTATTGA CGTCAATGGG CGGGGGTCGT TGGGCGGTCA GCCAGGCGGG
  841 CCATTTACCG TAAGTTATGT AACGCGGAAC TNCNTATATG GGCTATGAAC TNNTGACCCC
  901 GTAATTGATT ACTATTAATC NAATNNATTC NAAGTGAANT NNTGCTGNCC NNGNANTAAA
  961 CCCGAAAATT TTGAATTTTT TGNNNNTTGN TTTTTGNAAT TNNTTANTTT GNATGNNNNN
1021 TNNNNNNNAN NNNNNNNNN
//
Primer: IRES_R3
Region: attB2, insert 3'
Sequence file: RDB17497_A9Iob.seq check
>D06551A8_A9Io_3_IRES_R3_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNANGG GCGGNNCCGC GGCCGCTGCA GAATTCTCGA
   61 TGGCAACTAG AANGCNCNNN NNANGNTGAT CAGCGGGTTT ATCGAACCAC TTTGTACAAG
  121 AAAGCTGGGT CGGCGCGCCC ACCCTTTCAC TCCCACTTAT AGATGTGGAC CATCTTCTCT
  181 GTTAAGGTAG CCAAGTAGCT GTTACGGTTC ACCTCAAATG GGCAGATGTC CAACACAGGC
  241 TGATCGGTCT GTAGGTCCTG GAGCAACGAG CCACTGGCAG CATCCCACAG CAGGGCAGAA
  301 TTTGCTGCTT CATCCCCAGT ACACACCAGG ATGTTGCCAT CATTCTCTGG GCTTTGGAAA
  361 ATGGCATTTT TGGTCAATAG TTTGCAAGTA GGTCCTCCAA AAAATGTATG TACAGGCTGG
  421 CAGGAGCAGA TTGGATTTCC AGTGTCATCC AGTCGGTAGG ACATTTCCAT CAGCACACTT
  481 CGTATGGTGG TGTGATTTTT ATCAGGCCTG TAGGTCACAA GACAGTGCCG GGAGCTGTTC
  541 TCTGTCTGAA AGTCTATGCA GCCCCCTGGC TCCAAGGGCA GCACATGAGG CCAATGAGAA
  601 AAGTCCATTT TCTGNTCCCA GAATGAAGCA TCCTCCNAGG NTCCNGCCAG CACCCCACCN
  661 TATGGAAATG CNGCTGNNNA GCTCTGGGNA TGTATGANNN GNANNNCANN GGGCNTCNGG
721 NTTTCTGANC TNCTNNCTNN NGNNCANNAN NNNNNNNNN
//
Primer: hRFWD3-N_F
Region: insert mid (C315A)
Sequence file: RDB17497_A9Ioc.seq check
>D06551A8_A9Io_3_hRFWD3-N_F_F04_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CTGANAGTTG CAGGTGTCTA GTAGTTCTGA TTCTGACAGT
   61 GACAGCTCTG CAGAGTATGG AGGGGTTGTT GACCAGGCAG AGGAATCTGG AGCTGTCATT
  121 TTAGAAGAGC AACTAGCAGG TGTCTCAGCA GAGCAAGAAG TTACATGTAT CGATGGAGGC
  181 AAGACCCTCC CCAAACAGCC ATCTCCCCAG AAGTCTGAGC CTCTGCTACC TTCTGCTTCT
  241 ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG
  301 GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGGC CATTTCCACG
  361 TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC
  421 ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG
  481 AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG
  541 TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA AGCTTCAAAG GCGTGTTCAG
  601 GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA
  661 GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC
  721 CAAAAGANCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT
  781 CTGANCTGCC TGGTGATATC ACAGCCTTCT CCTCANGCCT CTTTTCTTCC AGGCTTTGGT
  841 GTTAAGANGT TGAGTACTGC CNACATGAAG AGCAGNCAGT ACATTCCGAT GCATGNCAAA
  901 CAGATNCGNG GNNNNNCGTT TANCAGTNAN CTCANANGCT TGCTANTCTC TGCTTNCNNA
  961 GANNACACTA NTAAANNGAN NANNCNNNNN NNNANTANCG NNNNNCANNN NTNNNNNNGN
1021 NNNNNNNNN
//
Primer: HIVgp_R1
Region: HIV-1 psi, 5' LTR
Sequence file: RDB17497_A9Iod.seq check
>D06551A8_A9Io_3_HIVgp_R1_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN NCTCTTTTTT CTCTCTGCAC CNCTCTTCTC TTTGCCTTGG
   61 TGGGTGCTAC TCCTAATGGT TCAATTTTTA CTACTTTATA TTTATATAAT TCACTTCTCC
  121 AATTGTCCCT CATATCTCCT CCTCCAGGTC TGAAGATCAG CGGCCGGCCG CTTGCTGTGC
  181 GGTGGTCTTA CTTTTGTTTT GCTCTTCCTC TATCTTGTCT AAAGCTTCCT TGGTGTCTTT
  241 TATCTCTATC CTTTGATGCA CACAATAGAG GGTTGCTACT GTATTATATA ATGATCTAAG
  301 TTCTTCTGAT CCTGTCTGAA GGGATGGTTG TAGCTGTCCC AGTATTTGTC TACAGCCTTC
  361 TGATGTTTCT AACAGGCCAG GATTAACTGC GAATCGTTCT AGCTCCCTGC TTGCCCATAC
  421 TATATGTTTT AATTTATATT TTTTCTTTCC CCCTGGCCTT AACCGAATTT TTTCCCATCG
  481 CGATCTAATT CTCCCCCGCT TAATACTGAC GCTCTCGCAC CCATCTCTCT CCTTCTAGCC
  541 TCCGCTAGTC AAAATTTTTG GCGTACTCAC CAGTCGCCGC CCCTCGCCTC TTGCCGTGCG
  601 CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA GAGCTCCTCT GGTTTCCCTT TCGCTTTCAA
  661 GTCCCTGTTC GGGCGCCACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGANGGAT
  721 CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG
  781 CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTNCCA GGCTCAGATC
  841 TGGTCTAACC AGAGAGANCC AGTACANNNA AAACGCGCTG CTTATATANA NCTNCCACCG
  901 TANNCGCCNA CCGNCCNTTT GCGTCNNTGG GGGCGGNNTT GNTACNANNN TTNNGAANNT
  961 CCCGNNGANT TNNGNTGNCN AAACNANNTC CNNTTGNCNN CNANGGGGGN GNANNNTNNG
1021 NAAATCCCCN NNNANNNNAN NNNNNNNTNN NNNNCCNNNN NNNNNN
//
Primer: cPPT+CTS_v2_F
Region: cPPT_CTS, CMV en 2, CMV pro 2
Sequence file: RDB17497_A9Ioe.seq check
>D06551A8_A9Io_3_cPPT+CTS_v2_F_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNACNAA AACNAATTAC NAAAATTCAA AATTTTCGGG
   61 TTTATTACAG GGACAGCAGA AATTCACTTT GAATTAATTC GATTAATAGT AATCAATTAC
  121 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG
  181 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC
  241 CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC
  301 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA
  361 TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC
  421 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA
  481 CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA
  541 CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA
  601 CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
  661 AGCTGGTTTA GTGAACCGNN NNATCCGCTA GGGAGACCCA ANCTGGCTAG CACCATGGAT
  721 TATAAAGATG ATGACGATAA GCATGGTGGT TCTGGTCCAA GCTATCAAAC AAGTTTGTAC
  781 AAAAAAGCAG GCTCCNCGGC CGCCCCCTTC ACCATGGCTC ATGAAGCAAT GGAATATGAT
  841 GTTCANGTGC AGTTAAATCA TGCCGAACAA CAGCCAGCTN CNGCTGGCAT GNNNNNNANN
  901 NNNNNGGGNA NCANCCCTCC TCCNNCCNGN NCCTGNNGAT GNGGTCAGCA GCCAGGGGGN
  961 ANCATCCNTC NNNNNNCNNN NTNNTGNNNN NNNGNTCANC AGNCNNANNN NNNNCNCCNN
1021 NNNTNNNNNN NNNN
//
Primer: IRES-F
Region: BSD, WPRE
Sequence file: RDB17497_A9Iof.seq check
>D06551A8_A9Io_3_IRES-F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTCTAGGCCC CCCGAACCAC
   61 GGGGANGTGG TTTTCCTTTG AAAAACACGA TGATAATATG GCCACAACCN TNGNCAANCC
  121 TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC
  181 CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC
  241 TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC
  301 TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG
  361 GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT
  421 CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT
  481 GCCCTCTGGT TATGTGTGGG AGGGCTAAGT CGAGCCTCTA GAGTTAACAT CGAGGGATCA
  541 AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA
  601 ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA
  661 TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT
  721 ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG
  781 CAACCCCCAC TGGTTGGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GNACTTTCGC
  841 TTTNCCCCTN CCTATTGCCA CGGCNGNANC TCATCGCCGC CTGNCTTGCC CGCTGCTGGA
  901 NANGGGCTCN GGCTGNTGGG CACTGACAAT TNCGTGGNGT TGTCNGGGGA NNCTNANGTC
  961 CTTTCCATGG CNGCTCNCCN GNNNNGNCNC NNGGNNNNCT GCNNGGGANN NNNTTCNGNT
1021 ANNNCCCNTN NNGNNNNTCA NNNNNNNNNN
//
Primer: f1ori_R3
Region: bGH p(A), 3' LTR
Sequence file: RDB17497_A9Iog.seq check
>D06551A8_A9Io_3_f1ori_R3_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGCCTCN GAANCCNTAG AGCCCACCGC
   61 ATCCCCAGCA TGCCTGCTAT TGTCTTCCCN ATCCTCCCCC TTGCTGTCCT GCCCCACCCC
  121 ACCCCCCAGA ATAGAATGAC ACCTACTCAG ACAATGCGAT GCAATTTCCT CATTTTATTA
  181 GGAAAGGACA GTGGGAGTGG CACCTTCCAG GGTCAAGGAA GGCACGGGGG AGGGGCAAAC
  241 AACAGATGGC TGGCAACTAG AAGGCACAGT CGAGGCTGAT CAGCGGGTTT AAACGGGCCC
  301 TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC
  361 ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA
  421 GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC
  481 CAGTACAGTC CGGATGCAGC TCTCGGGCCA TGTGATGAAA TGCTAGGCGG CTGTCAAACC
  541 TCCACTCTAA TACTTCTCTC TCCGGGTCAT CCATCCCATG CAGGCTCACA GGGTGTAACA
  601 AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT CTACCTTCTC TTGCTCAACT GGTACTAGCT
  661 TGTAGCACCA TCCAAAGGTC AGTGGATATC TGATCCCTGG CCCTGGTGTG TAGTTCTGCC
  721 AATCAGGGAA GTAGCCTTGT GTGTGGTAGA TCCACAGATC AAGGATATCT TGTCTTCGTT
  781 GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT TTTCTTTTAA AAAGTGGCTN AGATCTACAG
  841 CTGCCTTGTA AGTCATTGGT CTTAAAGGTA CCTGANGTGT GACTGGAAAA CCCACCTCCT
  901 NCNNCTCTNN TGCTTCTAGC CNGGCACNAT CAGCNNTGGT AGCTGCTGTA TTGCTNCTTG
  961 TGATNGCTCC ATGTTTTTTC NNNNNNNCGA CGGTNTCGNN NNNNGGGNNG NNNGNNNNAN
1021 NNNNNNNNCN NNNNNGNNNN NGNNNGNNNN NNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, EM7 pro, BleoR, SV40 p(A)
Sequence file: RDB17497_A9Ioh.seq check
>D06551A8_A9Io_3_SV40pro_ori_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNCCTCTGCC TCTGANCTNT TCNNGAAGTA GTGAGGAGGC
   61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA
  121 TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA GTATAATACG
  181 ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC
  241 GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG
  301 TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC
  361 AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC
  421 TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA
  481 TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA
  541 ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCGATTCCA
  601 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA
  661 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG
  721 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
  781 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC
  841 CGTCGANCTC TAGCTAGAGC TTNNCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT
  901 GTTATCCGCT CNCNATTCCN CACNACATAC GAGCCGGAAG CATAAAGNGN AANGCCNGGG
  961 GGNGNCTAAT GAGNGANCNN NNNCNCATTA ATTGNGTTGC GCNNNCNNNN NNNNTNNNNN
1021 NNNGNNANNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

2020.12.24

GNP_filter3_RDBDEP_html_201222.pl