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pcDNA3.1 FLAG-hRFWD3 aa 1-278

Expression vector of N-terminal region of human ring finger and WD repeat domain 3 (RFWD3), aa 1-278, RAD51 interaction region.

Catalog number RDB17494
Resource name pcDNA3.1 FLAG-hRFWD3 aa 1-278
Clone info. Expression vector of N-terminal region of human ring finger and WD repeat domain 3 (RFWD3), aa 1-278, RAD51 interaction region, tagged with FLAG at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3.1/Zeo (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cells, Zeocin.
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
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External Database
Human RFWD3

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
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          Remarks
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
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          RDB17494 pcDNA3.1 FLAG-hRFWD3 aa 1-278 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17494_A9Iop1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, FLAG, attB1, insert 5'
          Sequence file: RDB17494_A9Ioa.seq check
          >D06551A5_A9Io_1_CMV_Forward_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTGNNGNN NNNGGGCGGT AGGCGTGTAC GGTGGGAGGT
             61 CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT
            121 TAATACGACT CACTATAGGG AGACCCAAGC TGGCTAGCAC CATGGATTAT AAAGATGATG
            181 ACGATAAGCA TGGTGGTTCT GGTCCAAGCT ATCAAACAAG TTTGTACAAA AAAGCAGGCT
            241 CCGCGGCCAC CATGGCTCAT GAAGCAATGG AATATGATGT TCAGGTGCAG TTAAATCATG
            301 CCGAACAACA GCCAGCTCCT GCTGGCATGG CCAGCAGCCA AGGGGGACCA GCCCTCCTCC
            361 AGCCTGTTCC TGCTGATGTG GTCAGCAGCC AGGGGGTACC ATCCATCCTC CAGCCAGCTC
            421 CTGCTGAGGT GATCAGCAGC CAAGCGACAC CACCCCTGCT CCAGCCTGCT CCGCAACTGT
            481 CTGTTGACCT GACAGAAGTG GAGGTCTTGG GAGAAGACAC TGTGGAGAAC ATCAATCCAA
            541 GAACTTCAGA ACAACATAGG CAGGGATCTG ATGGTAATCA CACCATCCCA GCATCTTCGT
            601 TGCATTCAAT GACCAACTTC ATCAGCGGAC TGCAGAGACT TCATGGCATG CTGGAATTCC
            661 TGAGACCTTC ATCTTCAAAC CACAGTGTAG GGCCAATGAG AACAAGAAGG AGGGTATCTG
            721 CTTCACGGAG GGCAAGAGCC GGANGGTCTC AGANGANAGA CAGTGCCAGG TTGAGAGCAC
            781 CATTGGATGC TTACTTTCAG GTGAGCAGGA NCCAGCCTGA CTTGCCNGCT ACCACTTATG
            841 ATTCAGAGAC TAGGAATCCT GTATCTGAAG AGTTGCAGGN GTCTAGTANN TCTGANTCTG
            901 ACAGTGNCNG CTCTGCANAN TATGGANGGG TTGTTGNNNA NGNAGANGAA TCTGNANCTG
            961 TCATTTTNGA ANANCANCTA NCNNNTGNCT CNNCANANNN NNNNNTNANN TGTATNNANN
          1021 GNANNNNNNN NCCNNNNNNN NNN
          //
          Primer: f1ori_R3
          Region: bGH p(A), attB2, insert 3'
          Sequence file: RDB17494_A9Iob.seq check
          >D06551A5_A9Io_1_f1ori_R3_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNTC TTTCCGCCTC NGAAGCCNTA GAGCCCNCCG
             61 CATCCCCAGC ATGCCTGCTA TTGTCTTCCC AATCCTCCCC CTTGCTGTCC TGCCCCACCC
            121 CACCCCCCAG AATAGAATGA CACCTACTCA GACAATGCGA TGCAATTTCC TCATTTTATT
            181 AGGAAAGGAC AGTGGGAGTG GCACCTTCCA GGGTCAAGGA AGGCACGGGG GAGGGGCAAA
            241 CAACAGATGG CTGGCAACTA GAAGGCACAG TCGAGGCTGA TCAGCGGGTT TATCGAACCA
            301 CTTTGTACAA GAAAGCTGGG TCGGCGCGCC CAAGCTTGAA TTAGAAGCAG AAGGTAGCAG
            361 AGGCTCAGAC TTCTGGGGAG ATGGCTGTTT GGGGAGGGTC TTGCCTCCAT CGATACATGT
            421 AACTTCTTGC TCTGCTGAGA CACCTGCTAG TTGCTCTTCT AAAATGACAG CTCCAGATTC
            481 CTCTGCCTGG TCAACAACCC CTCCATACTC TGCAGAGCTG TCACTGTCAG AATCAGAACT
            541 ACTAGACACC TGCAACTCTT CAGATACAGG ATTCCTAGTC TCTGAATCAT AAGTGGTAGC
            601 TGGCAAGTCA GGCTGGGTCC TGCTCACCTG AAAGTAAGCA TCCAATGGTG CTCTCAACCT
            661 GGCACTGTCT GTCCTCTGAG ACCCTCCGGC TCTTGCCCTC CGTGAAGCAG ATACCCTCCT
            721 TCTTGTTCTC ATTGGCCCTA CACTGTGGTT TGAAGATGAA GGTCTCAGGA ATTCCAGCAT
            781 GCCATGAAGT CTCTGCAGTC CGCTGATGAA GTTGGTCATT GAATGCAACG AAGATGCTGG
            841 GATGGTGTGA TTACCATCAG ATCCNTGCCT ATGTTGTTCT GANNTTCTTG GATTGATGTT
            901 CNCCACAGTG TCTTCNCCNN ANANCTCCAC TTCTGTCANG TCNACAGACA GTTGCGGANC
            961 NGGNTGGANC NGGGGTGGTG TCNNNNGGCN GCTGATCANN NCANCNNNNC NNNNNNGNNN
          1021 NNGNNNNGNN NNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori, BleoR
          Sequence file: RDB17494_A9Ioc.seq check
          >D06551A5_A9Io_1_SV40pro_ori_F_G02_20_ABI24.ab1
              1 NNNNNNNNNN NNCGNNGNNN CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
             61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
            121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC
            181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC
            241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG
            301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG
            361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG
            421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG
            481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC
            541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC
            601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC
            661 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT
            721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
            781 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG
            841 TCGANCTCTA NCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
            901 TATCCNCTCN CAATTNCNCA CAACATACNA NCCNGAANCA TAAAGNGNAA AGCCNGGGGN
            961 GNCNNATGAG TGAGCTNNCT CANNTTNNTN GNGTTGNNNN NNCNGNCNNC TTTNCAGNCN
          1021 GGNANNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl