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pcDNA3.1 FLAG-hRFWD3 aa 1-278

Expression vector of N-terminal region of human ring finger and WD repeat domain 3 (RFWD3), aa 1-278, RAD51 interaction region.

Catalog number RDB17494
Resource name pcDNA3.1 FLAG-hRFWD3 aa 1-278
Clone info. Expression vector of N-terminal region of human ring finger and WD repeat domain 3 (RFWD3), aa 1-278, RAD51 interaction region, tagged with FLAG at N-terminus.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3.1/Zeo (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cells, Zeocin.
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 
Other clones in our bank human RFWD3 (NCBI Gene 55159) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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RDB17494 pcDNA3.1 FLAG-hRFWD3 aa 1-278 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17494_A9Iop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, FLAG, attB1, insert 5'
Sequence file: RDB17494_A9Ioa.seq check
>D06551A5_A9Io_1_CMV_Forward_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTGNNGNN NNNGGGCGGT AGGCGTGTAC GGTGGGAGGT
   61 CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT
  121 TAATACGACT CACTATAGGG AGACCCAAGC TGGCTAGCAC CATGGATTAT AAAGATGATG
  181 ACGATAAGCA TGGTGGTTCT GGTCCAAGCT ATCAAACAAG TTTGTACAAA AAAGCAGGCT
  241 CCGCGGCCAC CATGGCTCAT GAAGCAATGG AATATGATGT TCAGGTGCAG TTAAATCATG
  301 CCGAACAACA GCCAGCTCCT GCTGGCATGG CCAGCAGCCA AGGGGGACCA GCCCTCCTCC
  361 AGCCTGTTCC TGCTGATGTG GTCAGCAGCC AGGGGGTACC ATCCATCCTC CAGCCAGCTC
  421 CTGCTGAGGT GATCAGCAGC CAAGCGACAC CACCCCTGCT CCAGCCTGCT CCGCAACTGT
  481 CTGTTGACCT GACAGAAGTG GAGGTCTTGG GAGAAGACAC TGTGGAGAAC ATCAATCCAA
  541 GAACTTCAGA ACAACATAGG CAGGGATCTG ATGGTAATCA CACCATCCCA GCATCTTCGT
  601 TGCATTCAAT GACCAACTTC ATCAGCGGAC TGCAGAGACT TCATGGCATG CTGGAATTCC
  661 TGAGACCTTC ATCTTCAAAC CACAGTGTAG GGCCAATGAG AACAAGAAGG AGGGTATCTG
  721 CTTCACGGAG GGCAAGAGCC GGANGGTCTC AGANGANAGA CAGTGCCAGG TTGAGAGCAC
  781 CATTGGATGC TTACTTTCAG GTGAGCAGGA NCCAGCCTGA CTTGCCNGCT ACCACTTATG
  841 ATTCAGAGAC TAGGAATCCT GTATCTGAAG AGTTGCAGGN GTCTAGTANN TCTGANTCTG
  901 ACAGTGNCNG CTCTGCANAN TATGGANGGG TTGTTGNNNA NGNAGANGAA TCTGNANCTG
  961 TCATTTTNGA ANANCANCTA NCNNNTGNCT CNNCANANNN NNNNNTNANN TGTATNNANN
1021 GNANNNNNNN NCCNNNNNNN NNN
//
Primer: f1ori_R3
Region: bGH p(A), attB2, insert 3'
Sequence file: RDB17494_A9Iob.seq check
>D06551A5_A9Io_1_f1ori_R3_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNTC TTTCCGCCTC NGAAGCCNTA GAGCCCNCCG
   61 CATCCCCAGC ATGCCTGCTA TTGTCTTCCC AATCCTCCCC CTTGCTGTCC TGCCCCACCC
  121 CACCCCCCAG AATAGAATGA CACCTACTCA GACAATGCGA TGCAATTTCC TCATTTTATT
  181 AGGAAAGGAC AGTGGGAGTG GCACCTTCCA GGGTCAAGGA AGGCACGGGG GAGGGGCAAA
  241 CAACAGATGG CTGGCAACTA GAAGGCACAG TCGAGGCTGA TCAGCGGGTT TATCGAACCA
  301 CTTTGTACAA GAAAGCTGGG TCGGCGCGCC CAAGCTTGAA TTAGAAGCAG AAGGTAGCAG
  361 AGGCTCAGAC TTCTGGGGAG ATGGCTGTTT GGGGAGGGTC TTGCCTCCAT CGATACATGT
  421 AACTTCTTGC TCTGCTGAGA CACCTGCTAG TTGCTCTTCT AAAATGACAG CTCCAGATTC
  481 CTCTGCCTGG TCAACAACCC CTCCATACTC TGCAGAGCTG TCACTGTCAG AATCAGAACT
  541 ACTAGACACC TGCAACTCTT CAGATACAGG ATTCCTAGTC TCTGAATCAT AAGTGGTAGC
  601 TGGCAAGTCA GGCTGGGTCC TGCTCACCTG AAAGTAAGCA TCCAATGGTG CTCTCAACCT
  661 GGCACTGTCT GTCCTCTGAG ACCCTCCGGC TCTTGCCCTC CGTGAAGCAG ATACCCTCCT
  721 TCTTGTTCTC ATTGGCCCTA CACTGTGGTT TGAAGATGAA GGTCTCAGGA ATTCCAGCAT
  781 GCCATGAAGT CTCTGCAGTC CGCTGATGAA GTTGGTCATT GAATGCAACG AAGATGCTGG
  841 GATGGTGTGA TTACCATCAG ATCCNTGCCT ATGTTGTTCT GANNTTCTTG GATTGATGTT
  901 CNCCACAGTG TCTTCNCCNN ANANCTCCAC TTCTGTCANG TCNACAGACA GTTGCGGANC
  961 NGGNTGGANC NGGGGTGGTG TCNNNNGGCN GCTGATCANN NCANCNNNNC NNNNNNGNNN
1021 NNGNNNNGNN NNNNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, BleoR
Sequence file: RDB17494_A9Ioc.seq check
>D06551A5_A9Io_1_SV40pro_ori_F_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNCGNNGNNN CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC
  181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC
  241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG
  301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG
  361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG
  421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG
  481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC
  541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC
  601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC
  661 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT
  721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
  781 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG
  841 TCGANCTCTA NCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
  901 TATCCNCTCN CAATTNCNCA CAACATACNA NCCNGAANCA TAAAGNGNAA AGCCNGGGGN
  961 GNCNNATGAG TGAGCTNNCT CANNTTNNTN GNGTTGNNNN NNCNGNCNNC TTTNCAGNCN
1021 GGNANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

2020.11.02

GNP_filter3_RDBDEP_html_200316.pl