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pcDNA3.1 FLAG-hRFWD3 aa 1-278 (#RDB17494)

Expression vector of N-terminal region of human ring finger and WD repeat domain 3 (RFWD3), aa 1-278, RAD51 interaction region.

Clone info. Expression vector of N-terminal region of human ring finger and WD repeat domain 3 (RFWD3), aa 1-278, RAD51 interaction region, tagged with FLAG at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3.1/Zeo (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cells, Zeocin.
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17494 pcDNA3.1 FLAG-hRFWD3 aa 1-278 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1 FLAG-hRFWD3 aa 1-278 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17494).

Reference section:

Inano, S., Sato, K., Katsuki, Y., Kobayashi, W., Tanaka, H., Nakajima, K., Nakada, S., Miyoshi, H., Knies, K., Takaori-Kondo, A., Schindler, D., Ishiai, M., Kurumizaka, H., Takata, M., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17494_A9Iop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, FLAG, attB1, insert 5'
Sequence file: RDB17494_A9Ioa.seq check
>D06551A5_A9Io_1_CMV_Forward_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTGNNGNN NNNGGGCGGT AGGCGTGTAC GGTGGGAGGT
   61 CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT
  121 TAATACGACT CACTATAGGG AGACCCAAGC TGGCTAGCAC CATGGATTAT AAAGATGATG
  181 ACGATAAGCA TGGTGGTTCT GGTCCAAGCT ATCAAACAAG TTTGTACAAA AAAGCAGGCT
  241 CCGCGGCCAC CATGGCTCAT GAAGCAATGG AATATGATGT TCAGGTGCAG TTAAATCATG
  301 CCGAACAACA GCCAGCTCCT GCTGGCATGG CCAGCAGCCA AGGGGGACCA GCCCTCCTCC
  361 AGCCTGTTCC TGCTGATGTG GTCAGCAGCC AGGGGGTACC ATCCATCCTC CAGCCAGCTC
  421 CTGCTGAGGT GATCAGCAGC CAAGCGACAC CACCCCTGCT CCAGCCTGCT CCGCAACTGT
  481 CTGTTGACCT GACAGAAGTG GAGGTCTTGG GAGAAGACAC TGTGGAGAAC ATCAATCCAA
  541 GAACTTCAGA ACAACATAGG CAGGGATCTG ATGGTAATCA CACCATCCCA GCATCTTCGT
  601 TGCATTCAAT GACCAACTTC ATCAGCGGAC TGCAGAGACT TCATGGCATG CTGGAATTCC
  661 TGAGACCTTC ATCTTCAAAC CACAGTGTAG GGCCAATGAG AACAAGAAGG AGGGTATCTG
  721 CTTCACGGAG GGCAAGAGCC GGANGGTCTC AGANGANAGA CAGTGCCAGG TTGAGAGCAC
  781 CATTGGATGC TTACTTTCAG GTGAGCAGGA NCCAGCCTGA CTTGCCNGCT ACCACTTATG
  841 ATTCAGAGAC TAGGAATCCT GTATCTGAAG AGTTGCAGGN GTCTAGTANN TCTGANTCTG
  901 ACAGTGNCNG CTCTGCANAN TATGGANGGG TTGTTGNNNA NGNAGANGAA TCTGNANCTG
  961 TCATTTTNGA ANANCANCTA NCNNNTGNCT CNNCANANNN NNNNNTNANN TGTATNNANN
1021 GNANNNNNNN NCCNNNNNNN NNN
//
Primer: f1ori_R3 (PR0142)
Region: bGH p(A), attB2, insert 3'
Sequence file: RDB17494_A9Iob.seq check
>D06551A5_A9Io_1_f1ori_R3_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNTC TTTCCGCCTC NGAAGCCNTA GAGCCCNCCG
   61 CATCCCCAGC ATGCCTGCTA TTGTCTTCCC AATCCTCCCC CTTGCTGTCC TGCCCCACCC
  121 CACCCCCCAG AATAGAATGA CACCTACTCA GACAATGCGA TGCAATTTCC TCATTTTATT
  181 AGGAAAGGAC AGTGGGAGTG GCACCTTCCA GGGTCAAGGA AGGCACGGGG GAGGGGCAAA
  241 CAACAGATGG CTGGCAACTA GAAGGCACAG TCGAGGCTGA TCAGCGGGTT TATCGAACCA
  301 CTTTGTACAA GAAAGCTGGG TCGGCGCGCC CAAGCTTGAA TTAGAAGCAG AAGGTAGCAG
  361 AGGCTCAGAC TTCTGGGGAG ATGGCTGTTT GGGGAGGGTC TTGCCTCCAT CGATACATGT
  421 AACTTCTTGC TCTGCTGAGA CACCTGCTAG TTGCTCTTCT AAAATGACAG CTCCAGATTC
  481 CTCTGCCTGG TCAACAACCC CTCCATACTC TGCAGAGCTG TCACTGTCAG AATCAGAACT
  541 ACTAGACACC TGCAACTCTT CAGATACAGG ATTCCTAGTC TCTGAATCAT AAGTGGTAGC
  601 TGGCAAGTCA GGCTGGGTCC TGCTCACCTG AAAGTAAGCA TCCAATGGTG CTCTCAACCT
  661 GGCACTGTCT GTCCTCTGAG ACCCTCCGGC TCTTGCCCTC CGTGAAGCAG ATACCCTCCT
  721 TCTTGTTCTC ATTGGCCCTA CACTGTGGTT TGAAGATGAA GGTCTCAGGA ATTCCAGCAT
  781 GCCATGAAGT CTCTGCAGTC CGCTGATGAA GTTGGTCATT GAATGCAACG AAGATGCTGG
  841 GATGGTGTGA TTACCATCAG ATCCNTGCCT ATGTTGTTCT GANNTTCTTG GATTGATGTT
  901 CNCCACAGTG TCTTCNCCNN ANANCTCCAC TTCTGTCANG TCNACAGACA GTTGCGGANC
  961 NGGNTGGANC NGGGGTGGTG TCNNNNGGCN GCTGATCANN NCANCNNNNC NNNNNNGNNN
1021 NNGNNNNGNN NNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, BleoR
Sequence file: RDB17494_A9Ioc.seq check
>D06551A5_A9Io_1_SV40pro_ori_F_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNCGNNGNNN CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC
  181 AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC
  241 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG
  301 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG
  361 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG
  421 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG
  481 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC
  541 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC
  601 GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC
  661 CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT
  721 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
  781 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG
  841 TCGANCTCTA NCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
  901 TATCCNCTCN CAATTNCNCA CAACATACNA NCCNGAANCA TAAAGNGNAA AGCCNGGGGN
  961 GNCNNATGAG TGAGCTNNCT CANNTTNNTN GNGTTGNNNN NNCNGNCNNC TTTNCAGNCN
1021 GGNANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]