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pcDNA3.1 FLAG-hRFWD3 I639K

Expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, I639K, chromatin localization-defective mutation.

Catalog number RDB17492
Resource name pcDNA3.1 FLAG-hRFWD3 I639K
Clone info. Expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, I639K, chromatin localization-defective mutation, tagged with FLAG at N-terminus.
Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R492K; AGG to AAG).
Vector backbone pcDNA3.1/Zeo (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cells, Zeocin.
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 
Other clones in our bank human RFWD3 (NCBI Gene 55159) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
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Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17492 pcDNA3.1 FLAG-hRFWD3 I639K Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

2020.02.14

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