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pcDNA3.1 FLAG-hRFWD3 C315A (#RDB17491)

Expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, C315A, RING finger domain mutation.

Clone info. Expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, C315A, RING finger domain mutation, tagged with FLAG at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone pcDNA3.1/Zeo (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cells, Zeocin.
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17491 pcDNA3.1 FLAG-hRFWD3 C315A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1 FLAG-hRFWD3 C315A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17491).

Reference section:

Inano, S., Sato, K., Katsuki, Y., Kobayashi, W., Tanaka, H., Nakajima, K., Nakada, S., Miyoshi, H., Knies, K., Takaori-Kondo, A., Schindler, D., Ishiai, M., Kurumizaka, H., Takata, M., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17491_A9Iop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, FLAG, attB1, insert 5'
Sequence file: RDB17491_A9Ioa.seq check
>D06551A2_A9Io_2_CMV_Forward_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTGGGCGG TAGGCGTNNN NANNNNNNGA
   61 GGGTCTATAT AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG
  121 AAATTAATAC GACTCACTAT AGGGAGACCC AAGCTGGCTA GCACCATGGA TTATAAAGAT
  181 GATGACGATA AGCATGGTGG TTCTGGTCCA AGCTATCAAA CAAGTTTGTA CAAAAAAGCA
  241 GGCTCCGCGG CCGCCCCCTT CACCATGGCT CATGAAGCAA TGGAATATGA TGTTCAGGTG
  301 CAGTTAAATC ATGCCGAACA ACAGCCAGCT CCTGCTGGCA TGGCCAGCAG CCAAGGGGGA
  361 CCAGCCCTCC TCCAGCCTGT TCCTGCTGAT GTGGTCAGCA GCCAGGGGGT ACCATCCATC
  421 CTCCAGCCAG CTCCTGCTGA GGTGATCAGC AGCCAAGCGA CACCACCCCT GCTCCAGCCT
  481 GCTCCGCAAC TGTCTGTTGA CCTGACAGAA GTGGAGGTCT TGGGAGAAGA CACTGTGGAG
  541 AACATCAATC CAAGAACTTC AGAACAACAT AGGCAGGGAT CTGATGGTAA TCACACCATC
  601 CCAGCATCTT CGTTGCATTC AATGACCAAC TTCATCAGCG GACTGCAGAG ACTTCATGGC
  661 ATGCTGGAAT TCCTGAGACC TTCATCTTCA AACCACAGTG TAGGGCCAAT GAGAACAAGA
  721 AGGAGGGTAT NNNNNNCACG GAGGGCAAGA GCCGGANGGT CTCAGAGGAC AGACAGTGCC
  781 AGGTTGANAG CACCATTGGA TGCTTACTTT CANGTGAGCA GGANCCAGCC TGANTTGCCA
  841 GCTACCACTT ATGATTCAGA NNNTAGGAAT CCTGTATCTG AAGANTTGCA GGTGTCTANT
  901 AGTTCTGATT CTGACAGNGA CAGCTCTGCN NANNATGGAN GGGTTGTTGN NCAGNCANAN
  961 GNNNNTGGAN CTGTCNTTTT AGNANNNNNN NNCTAGCNNG TGTNNNAGCN NNNCNANNAN
1021 NNTNACNTGT ATNGATGGAN NNNNNNNNCC TNNNNNNANC NNNNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGHp(A), attB2, insert 3'
Sequence file: RDB17491_A9Iob.seq check
>D06551A2_A9Io_2_BGH_rev2_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNGGNNAACN ACNGATGGCT GGNNNNNAGA
   61 AGGCACAGTC GAGGCTGATC AGCGGGTTTA TCGAACCACT TTGTACAAGA AAGCTGGGTC
  121 GGCGCGCCCA CCCTTTCACT CCCACTTATA GATGTGGACC ATCTTCTCTG TTAAGGTAGC
  181 CAAGTAGCTG TTACGGTTCA CCTCAAATGG GCAGATGTCC AACACAGGCT GATCGGTCTG
  241 TAGGTCCTGG AGCAACGAGC CACTGGCAGC ATCCCACAGC AGGGCAGAAT TTGCTGCTTC
  301 ATCCCCAGTA CACACCAGGA TGTTGCCATC ATTCTCTGGG CTTTGGAAAA TGGCATTTTT
  361 GGTCAATAGT TTGCAAGTAG GTCCTCCAAA AAATGTATGT NNNNNNNGGC AGGAGCAGAT
  421 TGGATTTCCA GTGTCATCCA GTCGGTAGGA CATTTCCATC AGCACACTTC GTATGGTGGT
  481 GTGATTTTTA TCAGGCCTGT AGGTCACAAG ACAGTGCCGG GAGCTGTTCT CTGTCTGAAA
  541 GTCTATGCAG CCCCCTGGCT CCAAGGGCAG CACATGAGGC CAATGAGAAA AGTCCATTTT
  601 CTGTTCCCAG AATGAAGCAT CCTCCAAGGT TCCAGCCAGC ACCCCACCAT ATGGAAATGC
  661 AGCTGAGGCA GCTCTGGGCA TGTATNANNN GGAGACCAGT GGGCATCTGG CTTTCTGAGC
  721 TACTAACTCC TGCACATGAC TGCTCGTGTT TCGCACGTCA TATACCAGAA TTGAACCATT
  781 GGCCAGTCCA GCATAGATGT AGTTAGCCTC ATCAAGACAC CAGCAACAGC TCCNGANAGG
  841 ACGTCCAGCA TTATNAGTCT GGANNNCNGT ATTTGTCTNN NGGCNGGNCA GTTNAATAGT
  901 GTTGTCTAGG GAANCANANA GTANCAAGCN TCTNANGNAA CTGCTAAACG CCNNNNCNCN
  961 GNTCTGTTTG NNNTGCATCN GNAANGTACN GACTGNTNTN NNNGTTGGCN NTNNNCNNCA
1021 TNNNANNNCN NANNNCNNNN NNNNAAAAGA GGNCNNGNNN NANNANNNNN NNNNN
//
Primer: hRFWD3-N_F (Pr0753)
Region: insert mid (C315A)
Sequence file: RDB17491_A9Ioc.seq check
>D06551A2_A9Io_2_hRFWD3-N_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTCTGAANA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA
   61 CAGTGACAGC TCTGCAGAGT ATGGAGGGGT TGTTGACCAG GCAGAGGAAT CTGGAGCTGT
  121 CATTTTAGAA GAGCAACTAG CAGGTGTCTC AGCAGAGCAA GAAGTTACAT GTATCGATGG
  181 AGGCAAGACC CTCCCCAAAC AGCCATCTCC CCAGAAGTCT GAGCCTCTGC TACCTTCTGC
  241 TTCTATGGAT GAGGAAGAAG GGGACACTTG TACAATATGT CTGGAACAGT GGACCAATGC
  301 TGGGGACCAC CGGCTCTCAG CATTACGCTG TGGGCATCTC TTTGGGTATA GGGCCATTTC
  361 CACGTGGCTT AAAGGACAAG TACGAAAATG TCCCCAGTGC AACAAGAAAG CCAGGCACAG
  421 TGACATTGTC GTCCTTTATG CCCGAACCCT GAGAGCTTTG GACACTAGTG AACAGGAGCG
  481 CATGAAAAGT TCCCTACTGA AGGAACAGAT GCTAAGGAAA CAGGCCGAGT TAGAATCAGC
  541 ACAGTGCCGA CTCCAACTGC AGGTCCTCAC TGATAAGTGC ACTAAGCTTC AAAGGCGTGT
  601 TCAGGACTTG CAAAAACTTA CGTCACATCA AAGTCAGAAT TTACAGCAAC CCAGGGGCTC
  661 CCAAGCATGG GTCCTGAGCT GCTCACCCTC CAGCCAGGGC CAGCACAAGC ACAAGTACCA
  721 CTTCCAAAAG ACCTTCACAG TATCTCAGGC AGGAAACTGC CGGATCATGG CATACTGTGA
  781 TGCTCTGAGC TGCCTGGTGA TATCACAGCC TTCTCCTCAN GCCTCTTTTC TTCCAGGCTT
  841 TNGTGTTAAG ATGTTGAGTA CTGCCAACAT GAANAGCANT CAGTACATTC CGATGCATGN
  901 CAAACAGATN CGNNNANNNN NGTTTANCAG TTANCTCANA NGCTTGCTNC TCTCTGCTTN
961 CCNANACNAC ACTATTAAAC TGNNNNGCNN NNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, bleoR, SV40 p(A)
Sequence file: RDB17491_A9Iod.seq check
>D06551A2_A9Io_2_SV40pro_ori_F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NNNNNNNNCT CTGCCTCTGN NCTATTCNNN ANNNNNGTGA
   61 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT
  121 TTCGGATCTG ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT
  181 AATACGACAA GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA
  241 CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG
  301 ACTTCGTGGA GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG
  361 CGGTCCAGGA CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG
  421 ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC
  481 CGGCCATGAC CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG
  541 CCGGCAACTG CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG
  601 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT
  661 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA
  721 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT
  781 TTTTTTCACT GCATTCNAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT
  841 GTATACCGTC GANCTCTAGC TNGAGCTTNN CGTAATCATG GTCATAGCTG TTNNCTGTGT
  901 GAAATTGNTA TNCNGCTCAC AATTCNNCAC AANATACGAN NCNGNAANNC NTAAAGNGTA
  961 ANNCCTGGGG TNNCNAATGA GTGANCTAAC TCACNTTNAN TTGNNGTTGC GNNCNCNGNC
1021 CNNCTTTNNN NNNNNGGNNA NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]