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pcDNA3.1 FLAG-hRFWD3 wt (#RDB17490)

Expression vector of human ring finger and WD repeat domain 3 (RFWD3) wild type.

Clone info. Expression vector of human ring finger and WD repeat domain 3 (RFWD3) wild type, tagged with FLAG at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone pcDNA3.1/Zeo (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cells, Zeocin.
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17490 pcDNA3.1 FLAG-hRFWD3 wt DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1 FLAG-hRFWD3 wt was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17490).

Reference section:

Inano, S., Sato, K., Katsuki, Y., Kobayashi, W., Tanaka, H., Nakajima, K., Nakada, S., Miyoshi, H., Knies, K., Takaori-Kondo, A., Schindler, D., Ishiai, M., Kurumizaka, H., Takata, M., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17490_A9Iop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, FLAG, attB1, insert 5'
Sequence file: RDB17490_A9Ioa.seq check
>D06551A1_A9Io_2_CMV_Forward_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGGCGGT ANGCGTGTAC GGNGGGAGGT
   61 CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT
  121 TAATACGACT CACTATAGGG AGACCCAAGC TGGCTAGCAC CATGGATTAT AAAGATGATG
  181 ACGATAAGCA TGGTGGTTCT GGTCCAAGCT ATCAAACAAG TTTGTACAAA AAAGCAGGCT
  241 CCGCGGCCGC CCCCTTCACC ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT
  301 TAAATCATGC CGAACAACAG CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG
  361 CCCTCCTCCA GCCTGTTCCT GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC
  421 AGCCAGCTCC TGCTGAGGTG ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC
  481 CGCAACTGTC TGTTGACCTG ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA
  541 TCAATCCAAG AACTTCAGAA CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG
  601 CATCTTCGTT GCATTCAATG ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC
  661 TGGAATTCCT GAGACCTTCA TCTTCAAACC ACAGTGTANG GCCAATGAGA ACAAGAAGGA
  721 GGGTATCTGC TTCACGGAGG GCAAGAGCCG GANGGTCTCA GAGGANAGAC AGTGCCAGGT
  781 TGAGAGCACC ATTGGATGCT TACTTTCANG TGANCANGAC CCAGCCTGAN TTGCCAGCTA
  841 CCACTTATGA NTCNGAGACT NNGAATCCTG TATCTGAAGA GTTGCANGTG TCTAGTAGTT
  901 TNTGANTCTG ANAGTGNNAG CTCTGCNNAG TATGGANGGG TTGNNNANCN NGNCNNANGA
961 ATCTGGANCT NNNNTTTNNN NNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGHp(A), attB2, insert 3'
Sequence file: RDB17490_A9Iob.seq check
>D06551A1_A9Io_2_BGH_rev2_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNGGGGGNNG GGCAAACAAC AGATGGCTGG CAACTAGAAG
   61 GCACAGTCGA GGCTGATCAG CGGGTTTATC GAACCACTTT GTACAAGAAA GCTGGGTCGG
  121 CGCGCCCACC CTTTCACTCC CACTTATAGA TGTGGACCAT CTTCTCTGTT AAGGTAGCCA
  181 AGTAGCTGTT ACGGTTCACC TCAAATGGGC AGATGTCCAA CACAGGCTGA TCGGTCTGTA
  241 GGTCCTGGAG CAACGAGCCA CTGGCAGCAT CCCACAGCAG GGCAGAATTT GCTGCTTCAT
  301 CCCCAGTACA CACCAGGATG TTGCCATCAT TCTCTGGGCT TTGGAAAATG GCATTTTTGG
  361 TCAATAGTTT GCAAGTAGGT CCTCCAAAAA ATGTATGTAC AGGCTGGCAG GAGCAGATTG
  421 GATTTCCAGT GTCATCCAGT CGGTAGGACA TTTCCATCAG CACACTTCGT ATGGTGGTGT
  481 GATTTTTATC AGGCCTGTAG GTCACAAGAC AGTGCCGGGA GCTGTTCTCT GTCTGAAAGT
  541 CTATGCAGCC CCCTGGCTCC AAGGGCAGCA CATGAGGCCA ATGAGAAAAG TCCATTTTCT
  601 GTTCCCAGAA TGAAGCATCC TCCAAGGTTC CAGCCAGCAC CCCACCATAT GGAAATGCAG
  661 CTGAGGCAGC TCTGGGCATG TATGACAGGG AGACCAGTGG GCATCTGGCT TTCTGAGCTA
  721 CTAACTCCTG CACATGACTG CTCGTGTTTC GCACGTCATA TACCAGAATT GAACCATTGG
  781 CCAGTCCAGC ATAGATGTAG TTAGCCTCAT CAAGACACCA GCAACAGCTC CAGACAGGAC
  841 GTCCAGCATT ATAAGTCTGG ACCACGGTAT TTGTCTCCNG GCTGGTCAGT TTAATAGTGT
  901 TGTCTAGGGA AGCANANAGT ANCANGCCTC TGANGNNACT GCTNAACGCC AGNCCACGGA
  961 TCTGTTTGNC NTGCATNGGA ATGTACTGNC TGNNCTTCAT GTTGGCAGTN NNCNNNNTCN
1021 TNACNNNNAA NNCNNNNNNN AAAANNNNCN NNNNNNNNN
//
Primer: hRFWD3-N_F (Pr0753)
Region: insert mid
Sequence file: RDB17490_A9Ioc.seq check
>D06551A1_A9Io_2_hRFWD3-N_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTCTGNNAN TTGCAGGTGT CTAGTAGTTC TGATTCTGAC
   61 AGTGACAGCT CTGCAGAGTA TGGAGGGGTT GTTGACCAGG CAGAGGAATC TGGAGCTGTC
  121 ATTTTAGAAG AGCAACTAGC AGGTGTCTCA GCAGAGCAAG AAGTTACATG TATCGATGGA
  181 GGCAAGACCC TCCCCAAACA GCCATCTCCC CAGAAGTCTG AGCCTCTGCT ACCTTCTGCT
  241 TCTATGGATG AGGAAGAAGG GGACACTTGT ACAATATGTC TGGAACAGTG GACCAATGCT
  301 GGGGACCACC GGCTCTCAGC ATTACGCTGT GGGCATCTCT TTGGGTATAG GTGCATTTCC
  361 ACGTGGCTTA AAGGACAAGT ACGAAAATGT CCCCAGTGCA ACAAGAAAGC CAGGCACAGT
  421 GACATTGTCG TCCTTTATGC CCGAACCCTG AGAGCTTTGG ACACTAGTGA ACAGGAGCGC
  481 ATGAAAAGTT CCCTACTGAA GGAACAGATG CTAAGGAAAC AGGCCGAGTT AGAATCAGCA
  541 CAGTGCCGAC TCCAACTGCA GGTCCTCACT GATAAGTGCA CTAAGCTTCA AAGGCGTGTT
  601 CAGGACTTGC AAAAACTTAC GTCACATCAA AGTCAGAATT TACAGCAACC CAGGGGCTCC
  661 CAAGCATGGG TCCTGAGCTG CTCACCCTCC AGCCAGGGCC AGCACAAGCA CAAGTACCAC
  721 TTCCAAAAGA CCTTCACAGT ATCTCAGGCA GGAAACTGCC GGATCATGGC ATACTGTGAT
  781 GCTCTGAGCT GCCTGGTGAT ATCACAGCCT TCTCCTCANG CCTCTTTTCT TCCNGGCTTT
  841 NGTGTTAAGA TGTTGAGTAC TGCCAACATG AAGAGCAGTC AGTACATTCC GATGCATGGC
  901 AAACAGATNC GNNGANNGGC GTTTANCAGT TACCNCANAN GNTTGCTANT CTCNGCTTCC
961 NNANNNNNCA NTATTAAACT GANNANNCNN NNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, bleoR, SV40p(A)
Sequence file: RDB17490_A9Iod.seq check
>D06551A1_A9Io_2_SV40pro_ori_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNGNCGCC TCTGCCTCTG ANCTNTTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG
  121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA
  181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA
  241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA
  301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA
  361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA
  421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC
  481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG
  541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC
  601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT
  661 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA
  721 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT
  781 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC
  841 GACCTCTANC TAGAGCTTGG CGTAATCATG GNCATAGCTG TNTNCTGTGT GAAATTGTTA
  901 TCCGCNCNCN ATNNCNCACN ANATACNANC CNGNNNCATN AAGTGTAAAG NCTGGGGNGN
961 NNNANGANTG ANCTNNNNCN NATTNANTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658. [link to RRC of NBRP]