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pET-TuTs

Bacterial expression vector of E. coli EF-Tu and EF-Ts.

Catalog number RDB17457
Resource name pET-TuTs
Clone info. Bacterial expression vector of E. coli EF-Tu and EF-Ts.
The E. coli genes EF-Tu and EF-Ts were amplified from the E. coli A/lambda (sup pro/F+) genome by PCR. E. coli A/lambda was a kind gift from Dr. Y. Inokuchi (Teikyo University). Expression was confirmed by the depositor with SDS-PAGE and CBB staining.
Vector backbone pET21a (plasmid)
Selectable markers Amp
Gene/insert name E. coli EF-Tu, EF-Ts genomic
Depositor|Developer Ichihashi, Norikazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biosci. Bioeng. 101(5), 421-426, 2006)
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RDB17457 pET-TuTs DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full) RDB17457zzk01.seq provided by the depositor

Original reference

original Kita, H., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17457_A9Fop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2
Region: T7 pro, lacO, RBS, insert 5'
Sequence file: RDB17457_A9Foa.seq check
>D06471A4_A9Gj_1_pBR_BamHI_R2_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNANNCGNCC GGNNNANANG NNCGAGATCT
   61 CGATCCCGCG AAATTAATAC GACTCACTAT AGGGGAATTG TGAGCGGATA ACAATTCCCC
  121 TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGGCTGA AATTACCGCA
  181 TCCCTGGTAA AAGAGCTGCG TGAGCGTACT GGCGCAGGCA TGATGGATTG CAAAAAAGCA
  241 CTGACTGAAG CTAACGGCGA CATCGAGCTG GCAATCGAAA ACATGCGTAA GTCCGGTGCT
  301 ATTAAAGCAG CGAAAAAAGC AGGCAACGTT GCTGCTGACG GCGTGATCAA AACCAAAATC
  361 GACGGCAACT ACGGCATCAT TCTGGAAGTT AACTGCCAGA CTGACTTCGT TGCAAAAGAC
  421 GCTGGTTTCC AGGCGTTCGC AGACAAAGTT CTGGACGCAG CTGTTGCTGG CAAAATCACT
  481 GACGTTGAAG TTCTGAAAGC ACAGTTCGAA GAAGAACGTG TTGCGCTGGT AGCGAAAATT
  541 GGTGAAAACA TCAACATTCG CCGCGTTGCT GCGCTGGAAG GCGACGTTCT GGGTTCTTAT
  601 CAGCACGGTG CGCGTATCGG CGTTCTGGTT GCTGCTAAAG GCGCTGACGA AGAGCTGGTT
  661 AAACACATCG CTATGCACGT TGCTGCAAGC AAGCCAGAAT TCATCAAACC GGAAGANGTA
  721 TCCGCTGAAG TGGTAGAAAA AGAATACCAG GTACAGCTGG ATATCGCGAT GCAGTCTGGT
  781 AAGCCGAAAG AAATCGCAGA NAAAATGGTT GAANGCCGCA TGAAGAAATT CACCGGCGAA
  841 GTTTCTCTGA NNGGTCAGCC GTTCGTTATG NAACCNAGCA AAACNGTTGG NCAGCTGCTN
  901 NAAGANCATN ACGCTNAANN GNNNNGNNTT CNNNNGCTTC NANNNGGGNG NANGNNNNNN
961 NAANNNTNNA NNNNNNNNTN NNNNNNNNAN NNTNGNNNNN NNNNN
//
Primer: T7 terminator
Region: insert 3'
Sequence file: RDB17457_A9Fob.seq check
>D06471A4_A9Gj_1_T7 terminator_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN NTNNGCNGCC GGNNCTCNGT GGTGGTGGTG GTGGTGCTCG
   61 AGTTAGCTCA GAACTTTTGC TACAACGCCC GCGCCAACGG TACGGCCGCC TTCACGGATT
  121 GCGAAACGCA GACCGTCGTC CATCGCGATC GGGTGGATCA GGGTAACAAC CATTTTGATG
  181 TTGTCGCCCG GCATTACCAT CTCTACGCCT TCCGGCAGTT CGATGGTACC AGTCACGTCA
  241 GTAGTACGGA AGTAGAACTG CGGACGGTAG CCTTTGAAGA ACGGAGTATG ACGGCCGCCT
  301 TCATCTTTGG ACAGAATGTA CACTTCAGAT TCGAACTTGG TGTGCGGCTT GATGGTGCCC
  361 GGCTTAGCCA GTACCTGACC ACGTTCGATT TCTTCACGTT TGATACCACG CAGCAGAACA
  421 CCTACGTTCT CACCAGCACG GCCTTCGTCC AGCAGTTTGC GGAACATTTC AACGCCAGTA
  481 CAGGTAGACT TCTGAGTCTC TTTGATACCA ACGATTTCAA CTTCTTCACC AACTTTGATG
  541 ATACCGCGTT CTACACGACC GGTAACAACG GTACCACGAC CGGANNTGGA GAATACGTCT
  601 TCGATCGGCA GCAGGAACGG CTTGTCNATC GCACGCTCTG GTTCCGGAAT GTAAGAATCC
  661 AGGAAGCCNG CCNGTTCCNG GANTTTCGCT TCCCACTCTG CGTCGCCNTN CAGCGCTTTC
  721 NNANCANANN CNCNAACGAT NNGNNTGNCN NCGCCCNGGN ANNNCNNANT GANNNANAAN
781 TNCNNGANNN NNNNTTTNNN NNNNNNN
//
Primer: tuf-C_F1
Region: insert mid
Sequence file: RDB17457_A9Foc.seq check
>D06471A4_A9Gj_1_tuf-C_F1_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCNGGNNGNC GNANTCACGT GNNAAGTGTT GATGGTGANA
   61 CCACGAGCTT TTTCTTCCGG CGCGTTATCG ATCTGGTCGA ATGCGCGAGC AGCACCGCCG
  121 TAGGTTTTAG CCAGTACGGT AGTGATTGCA GCGGTCAGCG TTGTTTTACC ATGGTCAACG
  181 TGGCCGATAG TACCGACGTT AACGTGCGGT TTTGTACGTT CAAACTTTTC TTTAGACATA
  241 TGTATATCTC CTTCTTAAAG TTAAAAAGCT TGTCGACGGA GCTCGAATTC GGATCCTTAA
  301 GACTGCTTGG ACATCGCAGC AACTTCTGCT GCAAAGTCAG TCTCAACTTT CTCGATGCCT
  361 TCACCCACTT CGAAGCGGAT GAAGCCAGTC ACTTCAGCGT TATGCTCTTT CAGCAGCTGA
  421 CCAACAGTTT TGCTTGGTTC CATAACGAAC GGCTGACCGG TCAGAGAAAC TTCGCCGGTG
  481 AATTTCTTCA TGCGGCCTTC AACCATTTTC TCTGCGATTT CTTTCGGCTT ACCAGACTGC
  541 ATCGCGATAT CCAGCTGTAC CTGGTATTCT TTTTCTACCA CTTCAGCGGA TACGTCTTCC
  601 GGTTTGATGA ATTCTGGCTT GCTTGCAGCA ACGTGCATAG CGATGTGTTT AACCAGCTCT
  661 TCGTCAGCGC CTTTAGCAGC AACCAGAACG CCGATACGCG CACCGTGCTG ATAAGAANCC
  721 AGAACGTCGC CTTCCAGCGC AGCAACGCGG CGAATGTTGA TGTTTTCACC AATTTTCGCT
  781 ACCAGCGCAA CACGTTCTTC TTCGAACTGT GCTTTCAGAA CTTCAACGTC AGTGATTTTG
  841 CCAGCAACAG CTGCGTCCAG AACTTTGTCT GCGAACGCCN NNAAANNAGC GNCTTTTGNN
  901 ACGAANNCAG NCTGGNANNT AACTNNCAGA ANGATGCCGN ANNNGCCNNC NATTTTNNNT
961 TNGATCNCGN NNNNAGCAGN NNNNNNNNN
//
Primer: lacIpro_F2
Region: lacI pro, lacI
Sequence file: RDB17457_A9Fod.seq check
>D06471A4_A9Gj_1_lacIpro_F2_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGGN NNCGCATAAG GGNNNNCGTC GANNTCCCGG ANACCATCGA
   61 ATGGCGCAAA ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC AATTCAGGGT
  121 GGTGAATGTG AAACCAGTAA CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA
  181 GACCGTTTCC CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT
  241 GGAAGCGGCG ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG
  301 CAAACAGTCG TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA
  361 AATTGTCGCG GCGATTAAAT CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT
  421 GGTAGAACGA AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG
  481 CGTCAGTGGG CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC
  541 TGCCTGCACT AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG
  601 TATTATTTTC TCCCATGAAG ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG
  661 TCACCAGCAA ATCGCGCTGT TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC GTCTGCGTCT
  721 GGCTGGCTGG CATAAATATC TCACTCGCAA TCAAATTCAG CCGATAGCGG AACGGGAAGG
  781 CGACTNGAGT GCCATGTCCG GTTTTCAACA AACCATGCAA ATGCTGAATG ANGGCATCGT
  841 TCNNACTNCG ATGCTGGTTG CCNACGATCA GATGGCGCTG GGCGCAATGC GCGCCATTAC
  901 CGANNNCGGG CTGCNCGTNG GNGNNGATAT CTCNNNAGTG GNANANNNNN ATACCGNANN
961 ANNGNNCATG NTNATATCCN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kita, H., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472.

2021.09.13

GNP_filter3_RDBDEP_html_210618.pl