Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET-TuTs (#RDB17457)

Bacterial expression vector of E. coli EF-Tu and EF-Ts.

Clone info.	Bacterial expression vector of E. coli EF-Tu and EF-Ts.
Comment	The E. coli genes EF-Tu and EF-Ts were amplified from the E. coli A/lambda (sup pro/F+) genome by PCR. E. coli A/lambda was a kind gift from Dr. Y. Inokuchi (Teikyo University). Expression was confirmed by the depositor with SDS-PAGE and CBB staining.
Vector backbone	pET21a (plasmid)
Selectable markers	Amp
Gene/insert name	E. coli EF-Tu, EF-Ts genomic
Depositor|Developer	Ichihashi, Norikazu |

 

Sequence (full)	RDB17457zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biosci. Bioeng. 101(5), 421-426, 2006)

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB17457	pET-TuTs	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pET-TuTs was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17457).

Reference section:

Kita, H., Cho, J., Matsuura, T., Nakaishi, T., Taniguchi, I., Ichikawa, T., Shima, Y., Urabe, I., Yomo, T., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17457_A9Fop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2 (Pr0141) Region: T7 pro, lacO, RBS, insert 5' Sequence file: RDB17457_A9Foa.seq
>D06471A4_A9Gj_1_pBR_BamHI_R2_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNANNCGNCC GGNNNANANG NNCGAGATCT    61 CGATCCCGCG AAATTAATAC GACTCACTAT AGGGGAATTG TGAGCGGATA ACAATTCCCC   121 TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGGCTGA AATTACCGCA   181 TCCCTGGTAA AAGAGCTGCG TGAGCGTACT GGCGCAGGCA TGATGGATTG CAAAAAAGCA   241 CTGACTGAAG CTAACGGCGA CATCGAGCTG GCAATCGAAA ACATGCGTAA GTCCGGTGCT   301 ATTAAAGCAG CGAAAAAAGC AGGCAACGTT GCTGCTGACG GCGTGATCAA AACCAAAATC   361 GACGGCAACT ACGGCATCAT TCTGGAAGTT AACTGCCAGA CTGACTTCGT TGCAAAAGAC   421 GCTGGTTTCC AGGCGTTCGC AGACAAAGTT CTGGACGCAG CTGTTGCTGG CAAAATCACT   481 GACGTTGAAG TTCTGAAAGC ACAGTTCGAA GAAGAACGTG TTGCGCTGGT AGCGAAAATT   541 GGTGAAAACA TCAACATTCG CCGCGTTGCT GCGCTGGAAG GCGACGTTCT GGGTTCTTAT   601 CAGCACGGTG CGCGTATCGG CGTTCTGGTT GCTGCTAAAG GCGCTGACGA AGAGCTGGTT   661 AAACACATCG CTATGCACGT TGCTGCAAGC AAGCCAGAAT TCATCAAACC GGAAGANGTA   721 TCCGCTGAAG TGGTAGAAAA AGAATACCAG GTACAGCTGG ATATCGCGAT GCAGTCTGGT   781 AAGCCGAAAG AAATCGCAGA NAAAATGGTT GAANGCCGCA TGAAGAAATT CACCGGCGAA   841 GTTTCTCTGA NNGGTCAGCC GTTCGTTATG NAACCNAGCA AAACNGTTGG NCAGCTGCTN   901 NAAGANCATN ACGCTNAANN GNNNNGNNTT CNNNNGCTTC NANNNGGGNG NANGNNNNNN 961 NAANNNTNNA NNNNNNNNTN NNNNNNNNAN NNTNGNNNNN NNNNN //
Primer: T7 terminator (Pr0155) Region: insert 3' Sequence file: RDB17457_A9Fob.seq
>D06471A4_A9Gj_1_T7 terminator_C05_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNCNNN NTNNGCNGCC GGNNCTCNGT GGTGGTGGTG GTGGTGCTCG    61 AGTTAGCTCA GAACTTTTGC TACAACGCCC GCGCCAACGG TACGGCCGCC TTCACGGATT   121 GCGAAACGCA GACCGTCGTC CATCGCGATC GGGTGGATCA GGGTAACAAC CATTTTGATG   181 TTGTCGCCCG GCATTACCAT CTCTACGCCT TCCGGCAGTT CGATGGTACC AGTCACGTCA   241 GTAGTACGGA AGTAGAACTG CGGACGGTAG CCTTTGAAGA ACGGAGTATG ACGGCCGCCT   301 TCATCTTTGG ACAGAATGTA CACTTCAGAT TCGAACTTGG TGTGCGGCTT GATGGTGCCC   361 GGCTTAGCCA GTACCTGACC ACGTTCGATT TCTTCACGTT TGATACCACG CAGCAGAACA   421 CCTACGTTCT CACCAGCACG GCCTTCGTCC AGCAGTTTGC GGAACATTTC AACGCCAGTA   481 CAGGTAGACT TCTGAGTCTC TTTGATACCA ACGATTTCAA CTTCTTCACC AACTTTGATG   541 ATACCGCGTT CTACACGACC GGTAACAACG GTACCACGAC CGGANNTGGA GAATACGTCT   601 TCGATCGGCA GCAGGAACGG CTTGTCNATC GCACGCTCTG GTTCCGGAAT GTAAGAATCC   661 AGGAAGCCNG CCNGTTCCNG GANTTTCGCT TCCCACTCTG CGTCGCCNTN CAGCGCTTTC   721 NNANCANANN CNCNAACGAT NNGNNTGNCN NCGCCCNGGN ANNNCNNANT GANNNANAAN 781 TNCNNGANNN NNNNTTTNNN NNNNNNN //
Primer: tuf-C_F1 (Pr0657) Region: insert mid Sequence file: RDB17457_A9Foc.seq
>D06471A4_A9Gj_1_tuf-C_F1_D05_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNGGNNGNC GNANTCACGT GNNAAGTGTT GATGGTGANA    61 CCACGAGCTT TTTCTTCCGG CGCGTTATCG ATCTGGTCGA ATGCGCGAGC AGCACCGCCG   121 TAGGTTTTAG CCAGTACGGT AGTGATTGCA GCGGTCAGCG TTGTTTTACC ATGGTCAACG   181 TGGCCGATAG TACCGACGTT AACGTGCGGT TTTGTACGTT CAAACTTTTC TTTAGACATA   241 TGTATATCTC CTTCTTAAAG TTAAAAAGCT TGTCGACGGA GCTCGAATTC GGATCCTTAA   301 GACTGCTTGG ACATCGCAGC AACTTCTGCT GCAAAGTCAG TCTCAACTTT CTCGATGCCT   361 TCACCCACTT CGAAGCGGAT GAAGCCAGTC ACTTCAGCGT TATGCTCTTT CAGCAGCTGA   421 CCAACAGTTT TGCTTGGTTC CATAACGAAC GGCTGACCGG TCAGAGAAAC TTCGCCGGTG   481 AATTTCTTCA TGCGGCCTTC AACCATTTTC TCTGCGATTT CTTTCGGCTT ACCAGACTGC   541 ATCGCGATAT CCAGCTGTAC CTGGTATTCT TTTTCTACCA CTTCAGCGGA TACGTCTTCC   601 GGTTTGATGA ATTCTGGCTT GCTTGCAGCA ACGTGCATAG CGATGTGTTT AACCAGCTCT   661 TCGTCAGCGC CTTTAGCAGC AACCAGAACG CCGATACGCG CACCGTGCTG ATAAGAANCC   721 AGAACGTCGC CTTCCAGCGC AGCAACGCGG CGAATGTTGA TGTTTTCACC AATTTTCGCT   781 ACCAGCGCAA CACGTTCTTC TTCGAACTGT GCTTTCAGAA CTTCAACGTC AGTGATTTTG   841 CCAGCAACAG CTGCGTCCAG AACTTTGTCT GCGAACGCCN NNAAANNAGC GNCTTTTGNN   901 ACGAANNCAG NCTGGNANNT AACTNNCAGA ANGATGCCGN ANNNGCCNNC NATTTTNNNT 961 TNGATCNCGN NNNNAGCAGN NNNNNNNNN //
Primer: lacIpro_F2 (Pr0647) Region: lacI pro, lacI Sequence file: RDB17457_A9Fod.seq
>D06471A4_A9Gj_1_lacIpro_F2_E05_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGGN NNCGCATAAG GGNNNNCGTC GANNTCCCGG ANACCATCGA    61 ATGGCGCAAA ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC AATTCAGGGT   121 GGTGAATGTG AAACCAGTAA CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA   181 GACCGTTTCC CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT   241 GGAAGCGGCG ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG   301 CAAACAGTCG TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA   361 AATTGTCGCG GCGATTAAAT CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT   421 GGTAGAACGA AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG   481 CGTCAGTGGG CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC   541 TGCCTGCACT AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG   601 TATTATTTTC TCCCATGAAG ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG   661 TCACCAGCAA ATCGCGCTGT TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC GTCTGCGTCT   721 GGCTGGCTGG CATAAATATC TCACTCGCAA TCAAATTCAG CCGATAGCGG AACGGGAAGG   781 CGACTNGAGT GCCATGTCCG GTTTTCAACA AACCATGCAA ATGCTGAATG ANGGCATCGT   841 TCNNACTNCG ATGCTGGTTG CCNACGATCA GATGGCGCTG GGCGCAATGC GCGCCATTAC   901 CGANNNCGGG CTGCNCGTNG GNGNNGATAT CTCNNNAGTG GNANANNNNN ATACCGNANN 961 ANNGNNCATG NTNATATCCN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Kita, H., Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit. J. Biosci. Bioeng. 101 (5): 421-426 (2006). PMID 16781472. [link to RRC of NBRP]

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E. coli EF-Tu, EF-Ts

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