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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pUC-phi29DNAP_clone6-wt-loxP (#RDB17456)

Plasmid clone of the transcription and translation-coupled DNA replication (TTcDR) system, harboring phi29 DNA polymerase coding region and loxP site.

Clone info. Plasmid clone of the transcription and translation-coupled DNA replication (TTcDR) system, harboring phi29 DNA polymerase coding region and loxP site. Please refer Fig.1 of Sakatani, Y. et al., Sci. Rep., 8 (1) :13089, 2018. Clone 6 contains mutations contributing to improved DNA replication.
Vector backbone pUC19 (plasmid)
Selectable markers Amp
Gene/insert name Bacteriophage phi29 DNA polymerase genomic
Depositor|Developer Ichihashi, Norikazu |
 
Sequence (full) RDB17456zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakatani, Y. et al., Sci. Rep. 8(1), 13089, 2018)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sakatani, Y. et al., Sci. Rep. 8(1), 13089, 2018)。

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RDB17456 pUC-phi29DNAP_clone6-wt-loxP DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pUC-phi29DNAP_clone6-wt-loxP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17456).

Reference section:

Sakatani, Y., Yomo, T., Ichihashi, N., Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination. Sci. Rep. 8 (1): 13089 (2018). PMID 30166584. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17456_A9Fop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410)
Region: T7 pro, lac O, RBS, insert 5'
Sequence file: RDB17456_A9Foa.seq check
>D06471A3_A9Fo_1_M13_-40_G04_19_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNGGN NNNCGGGGNN CCTCTAGAGT CGANCTGCAG GCATGCTAAT
   61 ACGACTCACT ATAGGGGGGT TGTGAGCGGA TAACAATCCC CCCCTAGAAA TAATTTTGTT
  121 TAACTTTAAG AAGGAGATAT ACATATGCCG AGAAAGATGT ATAGTTGTGA CTTTGAGACA
  181 ACTACTAAAG TGGAAGACTG TAGGGTATGG GCGTATGGTT ATATGAATAT AGAAGATCAC
  241 AGTGAGTACA AAATAGGTAA TAGCCTGGAT GAGTTTATGG CGTGGGTGTT GAAGGTACAA
  301 GCTGATCTAT ATTTCCATAA CCTCAAATTT GACGGAGCTT TTATCATTAA CTGGTTGGAA
  361 CGTAGTGGTT TTAAGTGGTC GGCTGACGGA TTGCCAAACA CATATAATAC GATCATATCT
  421 CGCATGGGAC AATGGTACAT GATTGATATA TGTTTAGGCT ACAAAGGGAA ACGTAAGATA
  481 CATACAGTGA TATATGACAG CTTAAAGAAA CTACCGTTTC CTGTTAAGAA GATAGCTAAA
  541 GACTTTAAAC TAACTGTTCT TAAAGGTGAT ATTGATTACC ACAAAGAAAG ACCAGTCGGC
  601 TATAAGATAA CACCCGAAGA ATACGCCTAT ATTAAAAACG ATATTCAGAT TATTGCGGAA
  661 GCTCTGTTAA TTCAATTCAA GCAAGGTTTA GACCGGATGA CAGCAGGCAG TGACAGTCTA
  721 AAAGGNTTCA AGGATATTAT AACCACTAAG AAATTCAAAA AGGTATTTCC TACATTGAGT
  781 CTTGGACTCG ATAANGAAGT GAGATACGCC TATAGAGGTG GTTTTACATG GNTAAATGAT
  841 ANGNTCAAAG AAAAAGAAAT CGGAGAANGN ATGGTCTTCN ATGTTNATAN NCTATATCCT
  901 GCACAGATGT ATAGNNNTCT NCTNNCNTAC NNNNNNNCTA TNGNANTNNN NGGTNAANAC
961 NNTNGGNNNN NNNNNN
//
Primer: pBAC_lac_R (Pr0545)
Region: loxP, insert 3'
Sequence file: RDB17456_A9Fob.seq check
>D06471A3_A9Fo_1_pBAC_lac_R_H04_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NTNNGCGGNN AACTTCGTAT AATGTATGCT ATACGAAGTT
   61 ATTGCTCAGC GGATAACTTC GTATAATGTA TGCTATACGA AGTTATTGAC AGCAGCCAAC
  121 TCAGCTTCCT TTCGGGCTTC GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGCTCGAGT
  181 GCGGCCGCAA GCTTGTTGAC GGAGCTCGAA TTCATTATTT GATTGTGAAT GTGTTATCAA
  241 CCAGAACCAC CCCGCCCGGC ACTTGCACAG GCTTAGGCGT CATTTTCCGA CTGAATCCGA
  301 CTTTGAAATT CTCAAACGTA ACCTCTTTCT TAATCTTGTC AGTCATTCCC GCACATTTAA
  361 CACTAAATTT TATATCAGTG TAATCATCTG GACTACCTTC TACTAACTTA CCATCTACTT
  421 CTTTCATATA GATGTCTTGT ATATAGGTCT TCTGTCTCAA ATATTTAGCT CTTTTGAATG
  481 TACTTTCATG TGCCCAGTAT CCCAATTTCT TAGGGTCAAC TATATCTTTT ATTACATCAG
  541 GTATCTCTGT ACCCGTTAAA TGTATGCTGT CAGTATCACA GTATATTATC CGATCATAAC
  601 AAGCCTGTGC CGCTGTAATT GTCGTGTATC TAGCCCATGC AGTGATGAAA ACGCCCATAG
  661 GTGTATAAAC AGGGTCTTTT GTTTCCTCTT CTCCAAGTCT GAAACCTAGC GCCCCATTCT
  721 CTTTTNAATA ANGGACTTTC CCTGTAACAT CANGGTTACT AGCGAATTTA CCGTATAGAC
  781 TGTTTAACAT CAGTTTTGCT AGTTGCTTGA TCGCTCCTTC TGATGTCGNC TTGATGTACG
  841 TCCATTTATC TATNAANTCT TTAAACNAAC CTGTAGTTGC TTNNAATTTT AANNCNCTGA
  901 NATATTCNAC GNNATATAAA TCATANNGNT CTTNCNTTNN NTCTNNNNCN NCNTTTNNNN
961 NNNNNANNNC NGCTATNNNC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sakatani, Y., Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination. Sci. Rep. 8 (1): 13089 (2018). PMID 30166584. [link to RRC of NBRP]