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pUC-phi29DNAP_clone6-wt-loxP

Plasmid clone of the transcription and translation-coupled DNA replication (TTcDR) system, harboring phi29 DNA polymerase coding region and loxP site.

Catalog number RDB17456
Resource name pUC-phi29DNAP_clone6-wt-loxP
Clone info. Plasmid clone of the transcription and translation-coupled DNA replication (TTcDR) system, harboring phi29 DNA polymerase coding region and loxP site. Please refer Fig.1 of Sakatani, Y. et al., Sci. Rep., 8 (1) :13089, 2018. Clone 6 contains mutations contributing to improved DNA replication.
Vector backbone pUC19 (plasmid)
Selectable markers Amp
Gene/insert name Bacteriophage phi29 DNA polymerase genomic
Depositor Ichihashi, Norikazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakatani, Y. et al., Sci. Rep. 8(1), 13089, 2018)
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RDB17456 pUC-phi29DNAP_clone6-wt-loxP Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles


2019.08.01

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