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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pUC-phi29DNAP_clone6-wt-loxP

Plasmid clone of the transcription and translation-coupled DNA replication (TTcDR) system, harboring phi29 DNA polymerase coding region and loxP site.

Catalog number RDB17456
Resource name pUC-phi29DNAP_clone6-wt-loxP
Clone info. Plasmid clone of the transcription and translation-coupled DNA replication (TTcDR) system, harboring phi29 DNA polymerase coding region and loxP site. Please refer Fig.1 of Sakatani, Y. et al., Sci. Rep., 8 (1) :13089, 2018. Clone 6 contains mutations contributing to improved DNA replication.
Vector backbone pUC19 (plasmid)
Selectable markers Amp
Gene/insert name Bacteriophage phi29 DNA polymerase genomic
Depositor|Developer Ichihashi, Norikazu |
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External Database
Bacteriophage phi29 DNA polymerase
            Reference sequence
              
             
            Sequence (full) RDB17456zzk02.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakatani, Y. et al., Sci. Rep. 8(1), 13089, 2018)
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sakatani, Y. et al., Sci. Rep. 8(1), 13089, 2018)。
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            RDB17456 pUC-phi29DNAP_clone6-wt-loxP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Sakatani, Y., Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination. Sci. Rep. 8 (1): 13089 (2018). PMID 30166584.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17456_A9Fop1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Region: T7 pro, lac O, RBS, insert 5'
            Sequence file: RDB17456_A9Foa.seq check
            >D06471A3_A9Fo_1_M13_-40_G04_19_ABI24.ab1
                1 NNNNNNNNNN NNNNCNNGGN NNNCGGGGNN CCTCTAGAGT CGANCTGCAG GCATGCTAAT
               61 ACGACTCACT ATAGGGGGGT TGTGAGCGGA TAACAATCCC CCCCTAGAAA TAATTTTGTT
              121 TAACTTTAAG AAGGAGATAT ACATATGCCG AGAAAGATGT ATAGTTGTGA CTTTGAGACA
              181 ACTACTAAAG TGGAAGACTG TAGGGTATGG GCGTATGGTT ATATGAATAT AGAAGATCAC
              241 AGTGAGTACA AAATAGGTAA TAGCCTGGAT GAGTTTATGG CGTGGGTGTT GAAGGTACAA
              301 GCTGATCTAT ATTTCCATAA CCTCAAATTT GACGGAGCTT TTATCATTAA CTGGTTGGAA
              361 CGTAGTGGTT TTAAGTGGTC GGCTGACGGA TTGCCAAACA CATATAATAC GATCATATCT
              421 CGCATGGGAC AATGGTACAT GATTGATATA TGTTTAGGCT ACAAAGGGAA ACGTAAGATA
              481 CATACAGTGA TATATGACAG CTTAAAGAAA CTACCGTTTC CTGTTAAGAA GATAGCTAAA
              541 GACTTTAAAC TAACTGTTCT TAAAGGTGAT ATTGATTACC ACAAAGAAAG ACCAGTCGGC
              601 TATAAGATAA CACCCGAAGA ATACGCCTAT ATTAAAAACG ATATTCAGAT TATTGCGGAA
              661 GCTCTGTTAA TTCAATTCAA GCAAGGTTTA GACCGGATGA CAGCAGGCAG TGACAGTCTA
              721 AAAGGNTTCA AGGATATTAT AACCACTAAG AAATTCAAAA AGGTATTTCC TACATTGAGT
              781 CTTGGACTCG ATAANGAAGT GAGATACGCC TATAGAGGTG GTTTTACATG GNTAAATGAT
              841 ANGNTCAAAG AAAAAGAAAT CGGAGAANGN ATGGTCTTCN ATGTTNATAN NCTATATCCT
              901 GCACAGATGT ATAGNNNTCT NCTNNCNTAC NNNNNNNCTA TNGNANTNNN NGGTNAANAC
            961 NNTNGGNNNN NNNNNN
            //
            Primer: pBAC_lac_R
            Region: loxP, insert 3'
            Sequence file: RDB17456_A9Fob.seq check
            >D06471A3_A9Fo_1_pBAC_lac_R_H04_22_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNG NTNNGCGGNN AACTTCGTAT AATGTATGCT ATACGAAGTT
               61 ATTGCTCAGC GGATAACTTC GTATAATGTA TGCTATACGA AGTTATTGAC AGCAGCCAAC
              121 TCAGCTTCCT TTCGGGCTTC GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGCTCGAGT
              181 GCGGCCGCAA GCTTGTTGAC GGAGCTCGAA TTCATTATTT GATTGTGAAT GTGTTATCAA
              241 CCAGAACCAC CCCGCCCGGC ACTTGCACAG GCTTAGGCGT CATTTTCCGA CTGAATCCGA
              301 CTTTGAAATT CTCAAACGTA ACCTCTTTCT TAATCTTGTC AGTCATTCCC GCACATTTAA
              361 CACTAAATTT TATATCAGTG TAATCATCTG GACTACCTTC TACTAACTTA CCATCTACTT
              421 CTTTCATATA GATGTCTTGT ATATAGGTCT TCTGTCTCAA ATATTTAGCT CTTTTGAATG
              481 TACTTTCATG TGCCCAGTAT CCCAATTTCT TAGGGTCAAC TATATCTTTT ATTACATCAG
              541 GTATCTCTGT ACCCGTTAAA TGTATGCTGT CAGTATCACA GTATATTATC CGATCATAAC
              601 AAGCCTGTGC CGCTGTAATT GTCGTGTATC TAGCCCATGC AGTGATGAAA ACGCCCATAG
              661 GTGTATAAAC AGGGTCTTTT GTTTCCTCTT CTCCAAGTCT GAAACCTAGC GCCCCATTCT
              721 CTTTTNAATA ANGGACTTTC CCTGTAACAT CANGGTTACT AGCGAATTTA CCGTATAGAC
              781 TGTTTAACAT CAGTTTTGCT AGTTGCTTGA TCGCTCCTTC TGATGTCGNC TTGATGTACG
              841 TCCATTTATC TATNAANTCT TTAAACNAAC CTGTAGTTGC TTNNAATTTT AANNCNCTGA
              901 NATATTCNAC GNNATATAAA TCATANNGNT CTTNCNTTNN NTCTNNNNCN NCNTTTNNNN
            961 NNNNNANNNC NGCTATNNNC NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Sakatani, Y., Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination. Sci. Rep. 8 (1): 13089 (2018). PMID 30166584.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl