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pGBD-C1-Rab40AL-delta Cys-S28N

Expression vector of constitutive negative mutants of human Rab40AL (S28N) for yeast two-hybrid assay.

Catalog number RDB17444
Resource name pGBD-C1-Rab40AL-delta Cys-S28N
Clone info. Expression vector of constitutive negative mutants of human Rab40AL (S28N) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin, TRP1
Gene/insert name human RAB40AL cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
human RAB40AL
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aizawa, M. & Fukuda, M., J. Biol. Chem. 290, 22250-22261, 2015); (Ohishi, Y. et al. J. Biol. Chem. 294, 6912-6922, 2019)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aizawa, M. & Fukuda, M., J. Biol. Chem. 290, 22250-22261, 2015); (Ohishi, Y. et al. J. Biol. Chem. 294, 6912-6922, 2019)。
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          RDB17444 pGBD-C1-Rab40AL-delta Cys-S28N Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pGBD-C1-Rab40AL-delta Cys-S28N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17444).

          Reference section:

          Ohishi, Y., Kinoshita, R., Marubashi, S., Ishida, M., Fukuda, M., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Ohishi, Y., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268. [link to RRC of NBRP]
          reference Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634. [link to RRC of NBRP]

          2023.08.17

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