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pGBD-C1-Rab29-delta Cys-T21N (#RDB17426)

Expression vector of constitutive negative mutants of mouse Rab29 (T21N) for yeast two-hybrid assay.

Clone info. Expression vector of constitutive negative mutants of mouse Rab29 (T21N) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin, TRP1
Gene/insert name mouse Rab29 cDNA
Reference of insert sequence AB232625 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol, Cell 20, 2900-2908, 2009)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Tamura, K. et al. Mol. Biol, Cell 20, 2900-2908, 2009)。

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RDB17426 pGBD-C1-Rab29-delta Cys-T21N Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pGBD-C1-Rab29-delta Cys-T21N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17426).

Reference section:

Tamura, K., Ohbayashi, N., Maruta, Y., Kanno, E., Itoh, T., Fukuda, M., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]