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pGBD-C1-Rab11A-delta Cys-S25N (#RDB17406)

Expression vector of constitutive negative mutants of mouse Rab11A (S25N) for yeast two-hybrid assay.

Clone info. Expression vector of constitutive negative mutants of mouse Rab11A (S25N) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin, TRP1
Gene/insert name mouse Rab11a cDNA
Reference of insert sequence AB232605 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol, Cell 20, 2900-2908, 2009)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol, Cell 20, 2900-2908, 2009)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17406 pGBD-C1-Rab11A-delta Cys-S25N Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pGBD-C1-Rab11A-delta Cys-S25N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17406).

Reference section:

Tamura, K., Ohbayashi, N., Maruta, Y., Kanno, E., Itoh, T., Fukuda, M., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]