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pGBD-C1-Rab6D-delta Cys-T45N (#RDB17399)

Expression vector of constitutive negative mutants of human Rab6D (T45N) for yeast two-hybrid assay.

Clone info. Expression vector of constitutive negative mutants of human Rab6D (T45N) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
Comment Rab41(Rab6D) in NCBI one AA longer than that in NCBI
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin, TRP1
Gene/insert name human RAB41 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aizawa, M. & Fukuda, M., J. Biol. Chem. 290, 22250-22261, 2015); (Ohishi, Y. et al. J. Biol. Chem. 294, 6912-6922, 2019)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aizawa, M. & Fukuda, M., J. Biol. Chem. 290, 22250-22261, 2015); (Ohishi, Y. et al. J. Biol. Chem. 294, 6912-6922, 2019)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17399 pGBD-C1-Rab6D-delta Cys-T45N Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pGBD-C1-Rab6D-delta Cys-T45N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17399).

Reference section:

Ohishi, Y., Kinoshita, R., Marubashi, S., Ishida, M., Fukuda, M., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Ohishi, Y., The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis. J. Biol. Chem. 294 (17): 6912-6922 (2019). PMID 30837268. [link to RRC of NBRP]
reference Aizawa, M., Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology. J. Biol. Chem. 290 (36): 22250-22261 (2015). PMID 26209634. [link to RRC of NBRP]