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Ku80 px330

Expression vector of guide RNA for targeting human Ku80/XRCC5 exon 6.

Catalog number RDB17382
Resource name Ku80 px330
Alternative name Ku80 (XRCC5) exon6 pX330-U6-Chimeric_BB-CBh-hSpCas9
Clone info. Expression vector of guide RNA for targeting human X-ray repair cross complementing 5 (Ku80/XRCC5) exon 6 (5'-GATGGCCCCTTTCGCTTAGG-3') and human codon-optimized SpCas9.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r (E. coli)
Gene/insert name human XRCC5 dsOligo
Depositor|Developer Unoki, Motoko |
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External Database
human XRCC5
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
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          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
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          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB17382 Ku80 px330 Under QC test. Please contact us. DNA solution

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          How to cite this biological resource

          Materials & Methods section:

          The Ku80 px330 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17382).

          Reference section:

          Unoki, M., Funabiki, H., Velasco, G., Francastel, C., Sasaki, H., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

          2023.08.17

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