Please review the QC test results indicated by icon below as well as clone information before placing your order.

p3XFLAG-HELLS Q699R

Expression vector of human HELLS mutant, Q699R.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17377_A9Fhp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Region: CMV pro, 3xFLAG, insert 5' Sequence file: RDB17377_A9Fha.seq
>D06399A4_A9Fh_3_CMV_Forward_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TGGGCGGTAG GCGTGTACGG TGGGAGGTCT    61 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGAATTAACC ATGGACTACA AAGACCATGA   121 CGGNGATTAT AAAGATCATG ACATCGATTA CAAGGATGAC GATGACAAGC TTGCGGCCGC   181 GATGCCAGCG GAACGGCCCG CGGGCAGCGG CGGCTCGGAG GCTCCAGCAA TGGTTGAACA   241 ACTGGACACT GCTGTGATTA CCCCGGCCAT GCTAGAAGAG GAAGAACAGC TTGAAGCTGC   301 TGGACTAGAG AGAGAGCGGA AGATGCTGGA AAAGGCTCGC ATGTCTTGGG ATAGAGAGTC   361 GACAGAAATT CGGTACCGTA GACTTCAACA TTTGCTTGAA AAAAGCAATA TCTACTCCAA   421 ATTTTTATTG ACGAAAATGG AACAGCAACA ATTAGAGGAA CAGAAGAAGA AAGAAAAATT   481 GGAGAGAAAA AAGGAGTCTT TAAAAGTTAA AAAGGGTAAA AATTCAATTG ATGCAAGTGA   541 AGAGAAGCCA GTTATGAGGA AAAAAAGAGG AAGAGAAGAT GAATCATACA ATATTTCAGA   601 GGTCATGTCA AAAGAGGAAA TTTTGTCTGT GGCTAAAAAA AATAAAAAGG AGAATGAGGA   661 TGAAAACTCC TCCTCTACTA ATCTCTGTGT GGAAGATCTT CAGAAAAATA AAGATTCGAA   721 TAGTATAATT AAAGATAGAT TGTCTGAAAC GGTTAGGCAG AATACTAAAT TCTTTTTTGA   781 CCCAGTCCGG AAGTGTAATG GTCAGCCNGT ACCTTTTCAA CAACCAAAGC NNNNNNCTGG   841 AGGAGTGATG CGATGGTACC NAGTAGAANG NATGGAATGG CTTNNGATGC TTNGGGAAAA   901 TGGAATTAAT GGCNTTTTAG CANATGAAAT GGGATTGGGN NNGANAGTTC NNNGCATTGC   961 NNCTANTGNN TNNNTGANTC NNANANNNNT ACNNNGNANC TTTTNNNNNC NNNNNNNNNT 1021 NNNNNNNNNN //
Primer: hGHpolyA-R Region: hGHp(A), insert 3' Sequence file: RDB17377_A9Fhb.seq
>D06399A4_A9Fh_3_hGHpolyA-R_C02_08_ABI24.ab1     1 NNNNNNNNNN CCNNNNNNNN NNNNNNANNA TNGAAGGNNN CCTAGTCAGA CAAAATGATG    61 CAACTTAATT TTATTAGGAC AAGGCTGGTG GGCACTGGAG TGGCAACTTC CAGGGCCAGG   121 AGAGGCACTG GGGAGGGGTC ACAGGGATGC CACCCGGGAT CCTCTAGAGT CGACTGGTAC   181 CGATATCAGA TCTATCGATG AATTCGCGGC CGCTTAAAAC AAACATTCAG GACTGGAATC   241 TTCAGAATTT TCTAATATCT TGAATATCCC CATCTTCTCT TTAATTGGTC CTGAAGCATT   301 CATTTGATCA ATAAGATCAC TTCGATCTAA CAACAACTCT AGATCTTTAT CACTAATGAC   361 CTTCTCTCTT GATCCTTTTA TTTCCCTTTC ATAATCTCTA GATTTTAATA ATTCCATTAA   421 TTCCTTAGGA TCTAAGAAAT TCTTAGACAG ATTTAATCCA GACTGACCAC CTTTGAAATG   481 ATTTTTATGG ATGATCAACT TTTCCAGTTT CCTTTTAGCA GCTGCTCTTT CCACAATTTT   541 CTGATCGATA GTATTTGCTG TAACAAGGCG ATAAACAACA ACTGGCTTTG TCTGACCAAT   601 TCTATGACAT CTATCCTGGG CCTGAAGATC CGACCGGGGG TTCCAATCAC TATCATAAAT   661 GATAACTGTA TCTGCTGCAG TCAGATTAAT GCCCAGGCCA CCAGCTCGTG TACTCACTAA   721 GAAGATAAAC ACCTCTGGAT CCGTGTTGAA GCTGTGCATG TTTTTTTCTC TCTCTGAGTA   781 AGANATGGAC CCATCAAGCC TGCTGAAGTT GAAATCTCTG ANATGGCAGT AATCCATCAA   841 AATGTCCAAC ATGCTTGTCA TTTGTGAAAA AAGCAGCACC TTGTGANNTC TTTTTTTTTA   901 GTTCTGGNAG CATTCGATNC AAAATCAAGA ACTTNCCANA ATTTGNNANN NANTTCTTCN   961 TCGATCTTAA ATTCNNGTGT AACNGGGNCT ATNNGANNTT CNNNCNAATA TNGNANGANN  1021 ANNNCATTTN NNAAGNANCN TNANTNNNNT NNNGNNNNTN NNNNNTNNNN TNNNNNNNNN 1081 N //
Primer: SV40pro_F Region: NeoR/KanR Sequence file: RDB17377_A9Fhc.seq
>D06399A4_A9Fh_3_SV40pro_F_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTTTNNN TGNNNNNNNT TNGNNTCNNN    61 TTGGGANCTG ATCNAGAGAC AGGATGAGGA NCGTTTCGCA TGATTGAACA AGATGGATTG   121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG   181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT   241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA   301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG   361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT   421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT   481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG   541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA   601 NCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCNTGACC   661 CNTGNNNATG CCTGCTTGNC GAATATCATG GTGGAAANNG GNCGCTTTTC TGGATTCATC   721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCNNGACA TANCGTTGGC TACNCGNGAT   781 ATTGCTGAAG AGCTTGGCGG CGANTGGGGC TGACCGCTTN NTCNNGCTTT ACGGTATCNN   841 NGCTNNNNAT TCNNNNCGNA TCGCCNTCTA TCGCNTTCTT GNCNAGTTNN TNNTGANNNG 901 NNACTCTNNG GNTTCNAAAT GACCGNNNNN ANNNNANNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl