Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

p3XFLAG-HELLS Q699R (#RDB17377)

Expression vector of human HELLS mutant, Q699R.

Alternative name p3XFLAG-CMV10-HELLS Q699R
Clone info. Expression vector of human helicase lymphoid specific (HELLS) mutant, Q699R, tagged with 3 x FLAG at N-terminus. This mutation corresponds to the amino acid substitution identified in an ICF4 patient and is located in the helicase C-terminal domain. Structure, see Fig. S1 of Unoki, M. et al., J. Clin. Invest., 129 (1): 78-92, 2019.
Comment Expression was confirmed by the depositor with western blottingand RT-PCR.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human HELLS cDNA
Depositor|Developer Unoki, Motoko |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17377 p3XFLAG-HELLS Q699R DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3XFLAG-HELLS Q699R was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17377).

Reference section:

Unoki, M., Funabiki, H., Velasco, G., Francastel, C., Sasaki, H., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17377_A9Fhp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Region: CMV pro, 3xFLAG, insert 5'
Sequence file: RDB17377_A9Fha.seq check
>D06399A4_A9Fh_3_CMV_Forward_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGAATTAACC ATGGACTACA AAGACCATGA
  121 CGGNGATTAT AAAGATCATG ACATCGATTA CAAGGATGAC GATGACAAGC TTGCGGCCGC
  181 GATGCCAGCG GAACGGCCCG CGGGCAGCGG CGGCTCGGAG GCTCCAGCAA TGGTTGAACA
  241 ACTGGACACT GCTGTGATTA CCCCGGCCAT GCTAGAAGAG GAAGAACAGC TTGAAGCTGC
  301 TGGACTAGAG AGAGAGCGGA AGATGCTGGA AAAGGCTCGC ATGTCTTGGG ATAGAGAGTC
  361 GACAGAAATT CGGTACCGTA GACTTCAACA TTTGCTTGAA AAAAGCAATA TCTACTCCAA
  421 ATTTTTATTG ACGAAAATGG AACAGCAACA ATTAGAGGAA CAGAAGAAGA AAGAAAAATT
  481 GGAGAGAAAA AAGGAGTCTT TAAAAGTTAA AAAGGGTAAA AATTCAATTG ATGCAAGTGA
  541 AGAGAAGCCA GTTATGAGGA AAAAAAGAGG AAGAGAAGAT GAATCATACA ATATTTCAGA
  601 GGTCATGTCA AAAGAGGAAA TTTTGTCTGT GGCTAAAAAA AATAAAAAGG AGAATGAGGA
  661 TGAAAACTCC TCCTCTACTA ATCTCTGTGT GGAAGATCTT CAGAAAAATA AAGATTCGAA
  721 TAGTATAATT AAAGATAGAT TGTCTGAAAC GGTTAGGCAG AATACTAAAT TCTTTTTTGA
  781 CCCAGTCCGG AAGTGTAATG GTCAGCCNGT ACCTTTTCAA CAACCAAAGC NNNNNNCTGG
  841 AGGAGTGATG CGATGGTACC NAGTAGAANG NATGGAATGG CTTNNGATGC TTNGGGAAAA
  901 TGGAATTAAT GGCNTTTTAG CANATGAAAT GGGATTGGGN NNGANAGTTC NNNGCATTGC
  961 NNCTANTGNN TNNNTGANTC NNANANNNNT ACNNNGNANC TTTTNNNNNC NNNNNNNNNT
1021 NNNNNNNNNN
//
Primer: hGHpolyA-R ()
Region: hGHp(A), insert 3'
Sequence file: RDB17377_A9Fhb.seq check
>D06399A4_A9Fh_3_hGHpolyA-R_C02_08_ABI24.ab1
    1 NNNNNNNNNN CCNNNNNNNN NNNNNNANNA TNGAAGGNNN CCTAGTCAGA CAAAATGATG
   61 CAACTTAATT TTATTAGGAC AAGGCTGGTG GGCACTGGAG TGGCAACTTC CAGGGCCAGG
  121 AGAGGCACTG GGGAGGGGTC ACAGGGATGC CACCCGGGAT CCTCTAGAGT CGACTGGTAC
  181 CGATATCAGA TCTATCGATG AATTCGCGGC CGCTTAAAAC AAACATTCAG GACTGGAATC
  241 TTCAGAATTT TCTAATATCT TGAATATCCC CATCTTCTCT TTAATTGGTC CTGAAGCATT
  301 CATTTGATCA ATAAGATCAC TTCGATCTAA CAACAACTCT AGATCTTTAT CACTAATGAC
  361 CTTCTCTCTT GATCCTTTTA TTTCCCTTTC ATAATCTCTA GATTTTAATA ATTCCATTAA
  421 TTCCTTAGGA TCTAAGAAAT TCTTAGACAG ATTTAATCCA GACTGACCAC CTTTGAAATG
  481 ATTTTTATGG ATGATCAACT TTTCCAGTTT CCTTTTAGCA GCTGCTCTTT CCACAATTTT
  541 CTGATCGATA GTATTTGCTG TAACAAGGCG ATAAACAACA ACTGGCTTTG TCTGACCAAT
  601 TCTATGACAT CTATCCTGGG CCTGAAGATC CGACCGGGGG TTCCAATCAC TATCATAAAT
  661 GATAACTGTA TCTGCTGCAG TCAGATTAAT GCCCAGGCCA CCAGCTCGTG TACTCACTAA
  721 GAAGATAAAC ACCTCTGGAT CCGTGTTGAA GCTGTGCATG TTTTTTTCTC TCTCTGAGTA
  781 AGANATGGAC CCATCAAGCC TGCTGAAGTT GAAATCTCTG ANATGGCAGT AATCCATCAA
  841 AATGTCCAAC ATGCTTGTCA TTTGTGAAAA AAGCAGCACC TTGTGANNTC TTTTTTTTTA
  901 GTTCTGGNAG CATTCGATNC AAAATCAAGA ACTTNCCANA ATTTGNNANN NANTTCTTCN
  961 TCGATCTTAA ATTCNNGTGT AACNGGGNCT ATNNGANNTT CNNNCNAATA TNGNANGANN
 1021 ANNNCATTTN NNAAGNANCN TNANTNNNNT NNNGNNNNTN NNNNNTNNNN TNNNNNNNNN
1081 N
//
Primer: SV40pro_F ()
Region: NeoR/KanR
Sequence file: RDB17377_A9Fhc.seq check
>D06399A4_A9Fh_3_SV40pro_F_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTTTNNN TGNNNNNNNT TNGNNTCNNN
   61 TTGGGANCTG ATCNAGAGAC AGGATGAGGA NCGTTTCGCA TGATTGAACA AGATGGATTG
  121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
  181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
  241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
  301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
  361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
  421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
  481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
  541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
  601 NCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCNTGACC
  661 CNTGNNNATG CCTGCTTGNC GAATATCATG GTGGAAANNG GNCGCTTTTC TGGATTCATC
  721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCNNGACA TANCGTTGGC TACNCGNGAT
  781 ATTGCTGAAG AGCTTGGCGG CGANTGGGGC TGACCGCTTN NTCNNGCTTT ACGGTATCNN
  841 NGCTNNNNAT TCNNNNCGNA TCGCCNTCTA TCGCNTTCTT GNCNAGTTNN TNNTGANNNG
901 NNACTCTNNG GNTTCNAAAT GACCGNNNNN ANNNNANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]