Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

p3XFLAG-HELLS WT (#RDB17376)

Expression vector of human HELLS wild-type.

Alternative name p3XFLAG-CMV10-HELLS WT
Clone info. Expression vector of human helicase lymphoid specific (HELLS) wild-type, tagged with 3 x FLAG at N-terminus. Structure, see Fig. S1 of Unoki, M. et al., J. Clin. Invest., 129 (1): 78-92, 2019.
Comment Expression was confirmed by the depositor with western blottingand RT-PCR.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human HELLS cDNA
Depositor|Developer Unoki, Motoko |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17376 p3XFLAG-HELLS WT DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3XFLAG-HELLS WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17376).

Reference section:

Unoki, M., Funabiki, H., Velasco, G., Francastel, C., Sasaki, H., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17376_A9Fhp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Region: CMV pro, 3xFLAG, insert 5'
Sequence file: RDB17376_A9Fha.seq check
>D06399A3_A9Fh_1_CMV_Forward_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCNCN NNNNNNNNNN NGGGCGGTAG GCGTGNNNNG TGGGAGGTCT
   61 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGAATTAACC ATGGACTACA AAGACCATGA
  121 CGGTGATTAT AAAGATCATG ACATCGATTA CAAGGATGAC GATGACAAGC TTGCGGCCGC
  181 GATGCCAGCG GAACGGCCCG CGGGCAGCGG CGGCTCGGAG GCTCCAGCAA TGGTTGAACA
  241 ACTGGACACT GCTGTGATTA CCCCGGCCAT GCTAGAAGAG GAAGAACAGC TTGAAGCTGC
  301 TGGACTAGAG AGAGAGCGGA AGATGCTGGA AAAGGCTCGC ATGTCTTGGG ATAGAGAGTC
  361 GACAGAAATT CGGTACCGTA GACTTCAACA TTTGCTTGAA AAAAGCAATA TCTACTCCAA
  421 ATTTTTATTG ACGAAAATGG AACAGCAACA ATTAGAGGAA CAGAAGAAGA AAGAAAAATT
  481 GGAGAGAAAA AAGGAGTCTT TAAAAGTTAA AAAGGGTAAA AATTCAATTG ATGCAAGTGA
  541 AGAGAAGCCA GTTATGAGGA AAAAAAGAGG AAGAGAAGAT GAATCATACA ATATTTCANA
  601 GGTCATGTCA AAAGAGGAAA TTTTGTCTGT GGCTAAAAAA AATAAAAAGG AGAATGAGGA
  661 TGAAAACTCN TNNTCTACTA ATCTCTGTGT GGAAGATCTT CANAAAAATA AAGATTCNAA
  721 TAGTATAATT AAAGATAGAT TGTCTGAAAC GGTTAGGCAG AATACTAAAT TCTTTTTTGA
  781 NNNNNTCCGG AAGTGTAATG NTCAGNCNNT ACCTTTTCNA CNACCNAAGC ACTTCACTGG
  841 AGGANTGATG CGATGGTANN NAGTANAANG NNTGNNATGG NTNNNNNNGC TTTGGGAAAA
  901 TGNAATTAAT GGCATTTTTA NCNNATNAAN TGGGNNTNGG NNNNNANNNN NCNNNGCNTN
961 GCTNNNNNNN NNN
//
Primer: hGHpolyA-R ()
Region: hGHp(A), insert 3'
Sequence file: RDB17376_A9Fhb.seq check
>D06399A3_A9Fh_1_hGHpolyA-R_E01_05_ABI08.ab1
    1 CNNNNNNNNN NNNNNNNNNN NANANTANAG NAGGANACCT AGTCNNNACA AAATGATGCA
   61 ACTTAATTTT ATTAGGACAA GGCTGGTGGG CACTGGAGTG GCAACTTCCA GGGCCAGGAG
  121 AGGCACTGGG GAGGGGTCAC AGGGATGCCA CCCGGGATCC TCTAGAGTCG ACTGGTACCG
  181 ATATCAGATC TATCGATGAA TTCGCGGCCG CTTAAAACAA ACATTCAGGA CTGGAATCTT
  241 CAGAATTTTC TAATATCTTG AATATCCCCA TCTTCTCTTT AATTGGTCCT GAAGCATTCA
  301 TTTGATCAAT AAGATCACTT CGATCTAACA ACAACTCTAG ATCTTTATCA CTAATGACCT
  361 TCTCTCTTGA TCCTTTTATT TCCCTTTCAT AATCTCTAGA TTTTAATAAT TCCATTAATT
  421 CCTTAGGATC TAAGAAATTC TTAGACAGAT TTAATCCAGA CTGACCACCT TTGAAATGAT
  481 TTTTATGGAT GATCAACTTT TCCAGTTTCC TTTTAGCAGC TGCTCTTTCC ACAATTTTCT
  541 GATCGATAGT ATTTGCTGTA ACAAGGCGAT AAACAACAAC TGGCTTTGTC TGACCAATTC
  601 TATGACATCT ATCCTGGGCC TGAAGATCCG ACTGGGGGTT CCAATCACTA TCATAAATGA
  661 TAACTGTATC TGCTGCAGTC AGATTAATGC CCAGGCCACC AGCTCGTGTA CTCNCTAAGA
  721 AGANAAACAC CTCTGGATCC GTGTTGAAGC TGTGCATGTT TTTTTCTCTC TCTGANTAAG
  781 ACATGGACCC NTCAAGCCTG CTGAAGTTGA AATCTCTGAG ANGGCAGTAA TCCNTCNAAA
  841 TGTCCNANAT GCTTGTCATT TGTGAAAAAA GCANCNCCNT GNGANNTCTT TTTTTTANTT
  901 CTNGNCANNN TTCNATNCNA AATCNNNAAN NTNCNNGAAT TTGNNANNNN TTCNTCNTCN
961 ATNNNNNNNC NNNNGTNNNN NGNNNNANNN NNNANNNNNN NNNN
//
Primer: SV40pro_F ()
Region: NeoR/KanR
Sequence file: RDB17376_A9Fhc.seq check
>D06399A3_A9Fh_1_SV40pro_F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGNNGNTT TTTTGGANGA NNTGATCNGC
   61 TTGGGANCTG ATCAAGAGAC AGGNTGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
  121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
  181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
  241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
  301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
  361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
  421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
  481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
  541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
  601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
  661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGANTCNTC
  721 GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCANGACN TAGCGTTGGC TACCCGTGAT
  781 ATTGCTGAAN AGCTTGGCGG NNAATGGGCT GACCGCTTCC TCGTGCTTNA CGGTATCGCC
  841 GNTCNNNATT CNCANCGCAT CNCNNTCTAT CGNCNTNNNG ACNANTNCTT CTGANCGGGA
  901 NTCTNGGGGT TNNAAATGNN NNANNNANNN ACNNCCAACC NNNNNTCNCN ANNNTTCNAN
961 NNNNNNNGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]