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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-4T-3-Slac2-b-SHD

Expression vector of human Slac2-b/KIAA0624 (containing SHD) .

Catalog number RDB17372
Resource name pGEX-4T-3-Slac2-b-SHD
Clone info. Expression vector of human Slac2-b/KIAA0624 (containing the synaptotagmin-like protein homology domain (SHD)) tagged with GST at N-terminus, tac promoter.
A GTP-Rab27 trapper. Also known as EXPH5
Vector backbone pGEX-4T-3 (plasmid)
Selectable markers Amp^r.
Gene/insert name human EXPH5 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank human EXPH5 (NCBI Gene 23086) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002)
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RDB17372 pGEX-4T-3-Slac2-b-SHD DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Shirakawa, R., Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets. J. Biol. Chem. 279 (11): 10730-10737 (2004). PMID 14699162.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17372_A9Fap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Region: GST, thrombin site, insert
Sequence file: RDB17372_A9Faa.seq check
>D06377B7_A9Fa_3_pGEX-F2_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNTNNNNNN NGTATTGNAG CTATCCCACA AATTGATAAG TACTTGAAAT
   61 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC
  121 ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCATGTC ATTAAGTTTC TTAAATGACG
  181 AAGAGGCCAG GAAGATCCTT CAGGTGCTGG AAAGGAATGA GGAGTTACAG AGGGCCGAGA
  241 AGGACAGGAT CAGCAAACTT CAGAAGACAA AGAGGGATAT CAGATGGCTT CAGGGAGTGA
  301 CTGGTGAATG GTTTGAAGAA ATTCAAAGAA AAAAGTTTTG CAATGAAACA GATGTTAGCC
  361 AAATGTTAAA ACAACCACTT ACATACAGGC TAAGTAAGGA GATGGCAAAA AATGATTGAA
  421 TTCGATGCGG CCGCATCGTG ACTGACTGAC GATCTGCCTC GCGCGTTTCG GTGATGACGG
  481 TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC
  541 CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC
  601 CATGACCCAG TCACGTAGCG ATAGCGGAGT GTATAATTCT TGAAGACGAA AGGGCCTCGT
  661 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCANGTGG
  721 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA
  781 TATGTATCCG CTCATGANAN AATAACNNTG ATAAATGCTT CAATAATATT GAAAAANGAA
  841 GANTATGAGT ATTCAACATT TCCGNGNCGC CNNTNNNCCC NTTTTTTGCN GNATTTNGCC
  901 TNNCNGTTTT NGCNCACCCA GAAACGCNNG NGAAAGTAAA ANANGNTNNN NNNCAGNNNG
961 NNGNNCNANN GGGNNACNTC NNNCNNNNNN NNNN
//
Primer: lacIpro-B_F
Region: lacI pro, lacI
Sequence file: RDB17372_A9Fab.seq check
>D06377B7_A9Fa_3_lacI pro-B_F_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNNNNNNGNN TCNNNNNNNG ANGAANCGGA AGAGCGCCTG
   61 ATGCGGTATT TTCTCCTTNN NNATCTGTGC GGTATTTCAC ACCGCATAAA TTCCGACACC
  121 ATCGAATGGC GCAAAACCTT TCGCGGTATG GCATGATAGC GCCCGGAAGA GAGTCAATTC
  181 AGGGTGGTGA ATGTGAAACC AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT
  241 TATCAGACCG TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA
  301 AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC ACAACAACTG
  361 GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA GTCTGGCCCT GCACGCGCCG
  421 TCGCAAATTG TCGCGGCGAT TAAATCTCGC GCCGATCAAC TGGGTGCCAG CGTGGTGGTG
  481 TCGATGGTAG AACGAAGCGG CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG
  541 CAACGCGTCA GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG
  601 GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA GACACCCATC
  661 AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG GCGTGGAGCA TCTGGTCGCA
  721 TTGGGTCACC AGCAAATCGC GCTGTTAGCG GGCCCATTAA GTTCTGTCTC GGCGCGTCTG
  781 NNNNTGGCTG GCTGGCATNA NTATCTCACT CNCAATCAAA TTCAGCCGAT ANCGGAACGG
  841 GAAGGCGNNT GGANTGCCNT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGNNGNC
  901 ATCGTTNCCA CTGCNATGCT NGTTGNCANC GATNANATGG CNCTNGGNCG CNATGCGCGN
  961 NANTNNNNNA GTCNNGGGCT GCNCNNTNGN TGCGGNNNTC TNNGNTNNNN NNNNACNNNN
1021 NANNNCNNAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shirakawa, R., Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets. J. Biol. Chem. 279 (11): 10730-10737 (2004). PMID 14699162.
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

2020.10.02

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