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pMRX-puro-EGFP-Rab6A (#RDB17361)

Retroviral expression vector of mouse Rab6A.

Clone info. Retroviral expression vector of mouse Rab6A tagged with EGFP at N-terminus.
Vector backbone pMRX-puro-EGFP (plasmid)
Selectable markers Amp^r (E. coli), puro^r (mammalian cells).
Gene/insert name mouse Rab6a cDNA
Reference of insert sequence AB232596 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Homma, Y. et al. J. Cell Biol., in press (doi: 10.1083/jcb.201810134), 2019)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17361 pMRX-puro-EGFP-Rab6A DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMRX-puro-EGFP-Rab6A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17361).

Reference section:

Homma, Y., Kinoshita, R., Kuchitsu, Y., Wawro, P.S., Marubashi, S., Oguchi, M.E., Ishida, M., Fujita, N., Fukuda, M., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17361_A9Fap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: gag-M_F ()
Region: gag,pol region,EGFP
Sequence file: RDB17361_A9Faa.seq check
>D06377A5_A9Fa_1_gag-M_F_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNTNNNNNNN CCTAAGCCTC CGCCTCCTCT TCCTCCNTCC GCCCCGTCTC
   61 TCCCCCTTGA ACCTCCTCGT TCGACCCCGC CTCGATCCTC CCTTTATCCA GCCCTCACTC
  121 CTTCTCTAGG CGCCCCCATA TGGCCATATG AGATCTTATA TGGGGCACCC CCGCCCCTTG
  181 TAAACTTCCC TGACCCTGAC ATGACAAGAG TTACTAACAG CCCCTCTCTC CAAGCTCACT
  241 TACAGGCTCT CTACTTAGTC CAGCACGAAG TCTGGAGACC TCTGGCGGCA GCCTACCAAG
  301 AACAACTGGA CCGACCGGTG GTACCTCACC CTTACCGAGT CGGCGACACA GTGTGGGTCC
  361 GCCGACACCA GACTAAGAAC CTAGAACCTC GCTGGAAAGG ACCTTACACA GTCCTGCTGA
  421 CCACCCCCAC CGCCCTCAAA GTAGACGGCA TCGCAGCTTG GATACACGCC GCCCACGTGA
  481 AGGCTGCCGA CCCCGGGGGT GGACCATCCT CTAGACTGCC GGATCTGCCG CCACCATGGT
  541 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
  601 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
  661 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
  721 GANCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
  781 CGACTTCTTC AAGTCCGCCN TGCCCGANNG CTACGTCCAG GANCGCACCA TCTTCTTCAA
  841 NGACGANNGC AACTACNAGA NCCGCGCCGA NGTGAAGTTC GAGGGCGANA CCCNNGNGAA
  901 NCGCATCGNN CTGAANNGNN ATCGACTNNN NNNAGGANNG NAACNTCCTG GGGNCANANG
  961 CNGGANTANN ACTACNNNNG NCNNNANNNC TATATCNTNG NNNNANNNGC ANAANAANNN
1021 NNTNNANNNN NNNNNN
//
Primer: IRES-R ()
Region: IRES,insert,EGFP
Sequence file: RDB17361_A9Fab.seq check
>D06377A5_A9Fa_1_IRES-R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGTTAGGG GGGGGGGCGG ANTTTACGTA GCGGCCGCGA
   61 ATTCACTAGT GATTTTAGCA GGAACAGCCG CCTTCATTGA CTGGTTGCTC CTGAGGCTTT
  121 TCCAGTTTTA TGTCAATCAT GTCTTCTCTG CTTCTGTCCT GTGTGCTTTC CATTCCAGGT
  181 AAAGCTGCAG CAACACGCCG GAAAAGCTGC TTTACATTGT ATCCTGCTTT TGCACTGGTT
  241 TCAATAAACA TAACATTCAG CTCTTTGGCT TTCCTCTCTC CCTCCTCAAT TGACACTTGC
  301 CTCTTATCGG CAAGATCTGT TTTATTTCCT ACTAGCATGA TGATGACATC ACTTCCTCTC
  361 TCTGTTCTGA CATCATCAAT CCATTTTGTA GTTTGCTGGA ATGAGTTAAC ATTTGTGATA
  421 TCATAAACAA CAACTGCCAC AGTGGAGTCT CGAATGTAGC TAGGAATCAA GCTCCTGAAC
  481 CGCTCTTGAC CTGCTGTGTC CCATAATTGC AATCGCACGG TTCTATCCTC CAAGTACATT
  541 GTTTTTGATA AGAAGTCAAT GCCAATTGTT GCCTGATAGG TGTTGTCAAA ACTGTCATAC
  601 ATGAATCGGG TGATCAAGGA CGTCTTTCCA ACGCTCTGCT CTCCCAGGAA CACCAGCTTG
  661 AATTTCCTCA GCGGATTCCC GAAGTCTCCG CCCGCGGACA TGGATCCGAG TCCGGACTTG
  721 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGANCATG
  781 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC ANGGCGGANT GGGTGCTCAN GTAGTGGTTG
  841 TCGGGCAGCA GCACGGGGCC GTCGCCNANG GGGGTGNTCT GCTGGTAGTG GNCGGCGANC
  901 TGCACGCTGC CGNCCTCGAT GTTGTGNNGN NNCTTGNAGT TCACCTNNNT GCCNTNCNNC
961 TGCTNNNNNN NNNN
//
Primer: EGFP-C2 ()
Region: EGFP,insert
Sequence file: RDB17361_A9Fac.seq check
>D06377A5_A9Fa_1_EGFP-C2_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN CNCNTGGTCC TGCTGGAGTT CGTGANCGCC GCCGGGNTCA
   61 CTCTCGGCAT GGNCNAGCTG TACAAGTCCG GACTCGGATC CATGTCCGCG GGCGGAGACT
  121 TCGGGAATCC GCTGAGGAAA TTCAAGCTGG TGTTCCTGGG AGAGCAGAGC GTTGGAAAGA
  181 CGTCCTTGAT CACCCGATTC ATGTATGACA GTTTTGACAA CACCTATCAG GCAACAATTG
  241 GCATTGACTT CTTATCAAAA ACAATGTACT TGGAGGATAG AACCGTGCGA TTGCAATTAT
  301 GGGACACAGC AGGTCAAGAG CGGTTCAGGA GCTTGATTCC TAGCTACATT CGAGACTCCA
  361 CTGTGGCAGT TGTTGTTTAT GATATCACAA ATGTTAACTC ATTCCAGCAA ACTACAAAAT
  421 GGATTGATGA TGTCAGAACA GAGAGAGGAA GTGATGTCAT CATCATGCTA GTAGGAAATA
  481 AAACAGATCT TGCCGATAAG AGGCAAGTGT CAATTGAGGA GGGAGAGAGG AAAGCCAAAG
  541 AGCTGAATGT TATGTTTATT GAAACCAGTG CAAAAGCAGG ATACAATGTA AAGCAGCTTT
  601 TCCGGCGTGT TGCTGCAGCT TTACCTGGAA TGGAAAGCAC ACAGGACAGA AGCAGAGAAG
  661 ACATGATTGA CATAAAACTG GAAAAGCCTC ANGAGCAACC AGTCAATGAA GGCGGCTGTT
  721 CCTGCTAAAA TCACTAGTGA ATTCGCGGCC GCTACGTAAA TTCCGCCCCC CCCCCTAACG
  781 TTACTGGCCG AAGCCGCTTG GAATAANGNC GGTGTGCGTT TGTCTATATG TTATTTTCCA
  841 CCATATTGCC GTCTTTTGGC AATGTGAGGG CCCGGAAANC NGNNCCNGNC TTCTTGANNA
  901 NCATNNCTAG GGGTCTTTCC CCNNNCGCCN AANGNAANGN NANNNTCNGT TGANNGNCNN
961 GNANNNNNNC AGTNNCNTCN GNNANNCNTC NTNNNNNANN ANCNNNNNNN NNN
//
Primer: EGFP-N_rev ()
Region: EGFP,insert
Sequence file: RDB17361_A9Fad.seq check
>D06377A5_A9Fa_1_EGFP-N_rev_F10_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGGCG NNGCCNCCTA CGGNAAGCTG
   61 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  121 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  181 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  241 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  301 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  361 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  421 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  481 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  541 ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
  601 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCGGA
  661 TCCATGTCCG CGGGCGGAGA CTTCGGGAAT CCGCTGAGGA AATTCAAGCT GGTGTTCCTG
  721 GGAGAGCANA GCGTTGGAAA GACGTCCTTG ATCACCCGAT TCATGTATGA CAGTTTTGAC
  781 AACACCTATC NNGCAACAAN TGGCATTGAC TTCTTATCNA AAACNATGTA CTTGGANGAT
  841 AGAANNCGTG CNATTGCNAN TTATGGGACN CAGCNNNCAN GANCGGTTCA NGANCTTGAN
  901 NNNTAGCNNN NNTTCNAGAN TCCACNGTGG NCAGTTGTTG NTTTATGNAT ANNCNNCNAA
  961 NNGNTNNANT NNNTNNCNNG NCNAACNNNN AAANNNGNNN TNGNNNGNNN GNNCNNANNN
1021 NNNANNNNNN GNAANNNNNT NNNNATNNNN NNNNTNNNNN NNNNN
//
Primer: HCMVIEE_F2 ()
Region: CMV en,delta U3 5'LTR,MMLV psi
Sequence file: RDB17361_A9Fae.seq check
>D06377A5_A9Fa_1_HCMVIEE_F2_H03_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCNNNNNNN NNCTTACGGT AAATGGCCCG CCTGGCTGNN
   61 CGCCCAACGA CCCCCGCCCA TTGNCGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA
  121 TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG
  181 TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC
  241 CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT
  301 ACGTATTAGT CATCGCTATT ACCATGCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA
  361 AGGACCTGAA ATGACCCTGT GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT
  421 GTTCGCGCGC TTCTGCTCCC CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG
  481 CCAGTCCTCC GATTGACTGA GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG
  541 TTGCATCCGA CTTGTGGTCT CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC
  601 CCGTCAGCGG GGGTCTTTCA TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG
  661 ACCACCGACC CACCACCGGG AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG
  721 TCTAGTGTCT ATGACTGATT TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG
  781 TATCTGGCGG ANCCNTGGTG GAACTGANGA GTTCNGAACA CCCNGNNGCA ACCCNGGGAG
  841 ANGTCCCNNG GACTTCNGGG GGCCGTTTTT GTGNNCCGAN CTGANTCCNA AAATCCCNNN
  901 TCGNTTTNNG ACTCTTTGGT GCACCCCCCC TTNNANGAGG GANNNGTGGN NNTGGNAGGA
  961 NANNANNNNC TNAAANANNT NCCCGNNNNN NNNNTGAATT TTTNNNNTTN NNNNTNNGNN
1021 NNNNNAANNN CNNNNNNNNN N
//
Primer: pOTB7_R ()
Region: LTR,PuroR
Sequence file: RDB17361_A9Faf.seq check
>D06377A5_A9Fa_1_pOTB7_R_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNNNNNNNNN NNNNNNNNNN NNNNNNNGAN ACNTGCTGCA
   61 TGTGAAAGAC CCCCGCTGAC GGGTAGTCAA TCACTCAGAG GAGACCCTCC CAAGGAACAG
  121 CGAGACCACA AGTCGGATGC AACTGCAAGA GGGTTTATTG GATACACGGG TACCCGGGCG
  181 ACTCAGTCAA TCGGAGGACT GGCGCCCCGA GTGAGGGGTT GTGGGCTCTT TTATTGAGCT
  241 CGGGGAGCAG AAGCGCGCGA ACAGAAGCGA GAAGCGAACT GATTGGTTAG TTCAAATAAG
  301 GCACAGGGTC ATTTCAGGTC CTTGGGGCAC CCTGGAAACA TCTGATGGTT CTCTAGAAAC
  361 TGCTGAGGGC TGGACCGCAT CTGGGGACCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA
  421 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCTTGGCCCT
  481 GAGCCGGGGC AGGAACTGCT TACCACAGAT ATCCTGTTTG GCCCATATTC AGCTGTTCCA
  541 TCTGTTCCTG ACCTTGATCT GAACTTCTCT ATTCTCAGTT ATGTATTTTT CCATGCCTTG
  601 CAAAATGGCG TTACTTAAGC TAGCTTGCCA AACCTACAGG TGGGGTCTTT CATTCCCCCC
  661 TTTTTCTGGA GACTAAATAA AATCTTTTAT TTTATCGTCG AGCCATGGGG TCGTGCGCTC
  721 CTTTCGGTCG GGCGCTGCGG GTCGTGGGGC GGGCGTCANG CACCGGGCTT GCGGGTCATG
  781 CACCAGGTGC GCGGTCCTTC GGGCACCTCG ACGTCNGCGG TGACGGTGAA NCCGANCCGC
  841 TCGTANAANG GGANGTTGCG GGGCGCGGNN GTCTCCAGGA ANGNGGGCAC CCCGGCGCGC
  901 TCNGNCGCCT CCNCTCCNGG GANCNNGANN GCNCTGNCCA NANCCNNGCC CNGGTGGNNG
961 GGCGANANNC NNANNNGGNC AGNANNCNCN CNGGNTNCNT NNNNCNNNNN NNNNN
//
Primer: IRES-F ()
Region: IRES,PuroR
Sequence file: RDB17361_A9Fag.seq check
>D06377A5_A9Fa_1_IRES-F_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNTTNNGT CGAGGTTNNN AAAACGTCTA GGCCCCCCGA ACCACGGGGN
   61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGACC GAGTACAAGC
  121 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG
  181 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG
  241 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG
  301 TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG
  361 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC
  421 AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG
  481 CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC
  541 CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC
  601 GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG
  661 AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG CCCCACGACC
  721 CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGGCTC GACGATAAAA TAAAAGANTT
  781 TATTTAGTCT CCAGANAAAN GGGGGAATGA AAGANCCCAC CTGTANGTTT GGNAAGCTAG
  841 CTTAAGTAAC GCCATTTTGC AAGGNATGGA AAAATACATA ACTGANAATA GAGAANTTCA
  901 NATNNANGGT CNGGAACAGA TGGNACAGCT NNANNTGGGC CNAACAGGNT NTCTGTGGNA
  961 ANCAGTTNNN GNCCCGGNTC NNNNCNNNNN NCAGATGNNN NNNCNNNNNA TNGNNCNAAA
1021 CNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Homma, Y., Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. J. Cell Biol. 218 (6): 2035-2050 (2019). PMID 31072826. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]
user_report Etoh, K., Citrate metabolism controls the senescent microenvironment via the remodeling of pro-inflammatory enhancers. Cell Rep. 43 (8): 114496 (2024). PMID 39043191. [link to RRC of NBRP]