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pEF6-FLAG-human Syncrip (iso1) (#RDB17345)

Expression vector of human SYNCRIP (Isoform 1) tagged with FLAG at N-terminus.

Clone info. Expression vector of human synaptotagmin binding cytoplasmic RNA interacting protein (SYNCRIP) tagged with FLAG at N-terminus. Iso 1 (Isoform 1) is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself.
Vector backbone pEF6/myc-His (plasmid)
Selectable markers Amp^r (E. coli), BSD (mammalian cells).
Gene/insert name human SYNCRIP cDNA
Depositor|Developer Maruyama, Takashi |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Tatsushi Muta is requested.
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RDB17345 pEF6-FLAG-human Syncrip (iso1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF6-FLAG-human Syncrip (iso1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17345).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17345_A9Etp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1alpha pro, FLAG, insert5'
Sequence file: RDB17345_A9Eta.seq check
>D05962B1_A9Et_2_EF1a-C_F_B11_05_ABI24.ab1
    1 NNNNNNNNNN NNCNNNTTTG ANTTTGGNNC TTGGTTNNTT CTCNAGCCTC AGACAGTGGT
   61 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAGGAATT AGCTTGGTAC TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGGTA AGCTTGGTAC CGAGCTCGGA TCGCCACCAT
  181 GGACTACAAG GACGACGATG ACAAGGGATC CACCATGGCT ACAGAACATG TTAATGGAAA
  241 TGGTACTGAA GAGCCCATGG ATACTACTTC TGCAGTTATC CATTCAGAAA ATTTTCAGAC
  301 ATTGCTTGAT GCTGGTTTAC CACAGAAAGT TGCTGAAAAA CTAGATGAAA TTTACGTTGC
  361 AGGGCTAGTT GCACATAGTG ATTTAGATGA AAGAGCTATT GAAGCTTTAA AAGAGTTCAA
  421 TGAAGACGGT GCATTGGCAG TTCTTCAACA GTTTAAAGAC AGTGATCTCT CTCATGTTCA
  481 GAACAAAAGT GCCTTTTTAT GTGGAGTCAT GAAGACTTAC AGGCAGAGAG AAAAACAAGG
  541 GACCAAAGTA GCAGATTCTA GTAAAGGACC AGATGAGGCA AAAATTAAGG CACTCTTGGA
  601 AAGAACAGGC TACACACTTG ATGTGACCAC TGGACAGAGG AAGTATGGAG GACCACCTCC
  661 AGATTCCGTT TATTCAGGTC AGCAGCCTTC TGTTGGCACT GAGATATTTG TGGGAAAGAT
  721 CCCAAGAGAT CTATTTGAGG ANGAACTTGT TCCATTATTT GAGAAAGCTG GACCTATATG
  781 GGATCTTCGT CTAATGATGG ACCCACTCAC TGGTCTCAAT AGAGGTTATG CGTTTGTCAC
  841 TTTTTGNACN AAAGAAGCAG CTCNNGANGC TGTTAAACTG NATAATAATC ATGAAATTCG
  901 TTCTGGAAAA NNTATTGGNG NCNGCATCNC AGTNGNNNAC NANNNNCTTT TTTGTGGNCN
961 CTANNNCNNN NNNNAAAANN NANNNANNNN NNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: BGH p(A), insert3'
Sequence file: RDB17345_A9Etb.seq check
>D05962B1_A9Et_2_BGH_rev2_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNGGGGG NNGGGNNNNC NACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACTCAATG GTGATGGTGA TGATGACCGG TACGCGTAGA
  121 ATCGAGACCG AGGAGAGGGT TAGGGATAGG CTTACCTTCG AAGGGCCCTC TAGACTCGAG
  181 CGGCCGCCAC TGTGCTGGAT ATCTGCAGAA TTCCTACTTC CACTGTTGCC CAAAAGTATC
  241 CTGATAAAAC TCCTGGTTTT CAGATTTGTA ACCATAGTTA CCAGAATGAT CACCACCTTG
  301 GAGCGGTTGC TGAGCAATGG GTTGGGAGCC CCAGTTCTGA TTATTGGTCT GGCGCCGCTT
  361 GGAATCTGGC TGGTTGTACC CATCAGCTTT GCGCTTTCCT CCTACATTTC CACCGCGGCC
  421 ACCCCTCGCA CCACGTACCC CGCGGCCTCT TTGTTGTTGG GCACCTCCTC TCGCACCTCG
  481 AACGCCTCTT GCTGATCCAG GACCTCCTCT CTGTGAATAA CCGGCTCTAC CGCGGGGAGG
  541 AGCAGCCCCA CGACCTCTGG ATGGAGCAGC ACCCCTTGCT CCTCTACCAC CCCTTCCTCT
  601 AGCTCCAACT TGAAAATCTT CATAACCATA GTATGGATCT TCATATCCAC CACGATAGTT
  661 ATGGTAATCA TAACCATAAT AATCATAATA ATCTTCATAT CCATAATAAT CTGGAGGANA
  721 TCCATAACCA CCTCTACCTC CACGCCCTCG ANCTCTTGTT GGANGGGGCA TATGANGTGG
  781 ANCATAATAG TAGTAATCGT CATACATTTG ANTTTTTGCT GCTTGCCTCT GANCTTTTCN
  841 TTCNTTNCTT TTCTGATCTG GTGGNTTGGC AAAAACNATT TCAATATTTT CTCCNNNNNA
  901 GTCTTTGNCN TTCATTTCTT NCNTAGCCNT NNNAGCACNN TNNCGCNCAT CAAAATGNAN
961 GNNNNNNNNN N
//
Primer: SV40pro_F (Pr0110)
Region: SV40pro_ori, BSD
Sequence file: RDB17345_A9Etc.seq check
>D05962B1_A9Et_2_SV40pro_F_F04_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NANNNNTGAN GANGNTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
  121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
  181 CAAGCCTTTG TCTCAAGAAG AATCCACCCT CATTGAAAGA GCAACGGCTA CAATCAACAG
  241 CATCCCCATC TCTGAAGACT ACAGCGTCGC CAGCGCAGCT CTCTCTAGCG ACGGCCGCAT
  301 CTTCACTGGT GTCAATGTAT ATCATTTTAC TGGGGGACCT TGTGCAGAAC TCGTGGTGCT
  361 GGGCACTGCT GCTGCTGCGG CAGCTGGCAA CCTGACTTGT ATCGTCGCGA TCGGAAATGA
  421 GAACAGGGGC ATCTTGAGCC CCTGCGGACG GTGCCGACAG GTGCTTCTCG ATCTGCATCC
  481 TGGGATCAAA GCCATAGTGA AGGACAGTGA TGGACAGCCG ACGGCAGTTG GGATTCGTGA
  541 ATTGCTGCCC TCTGGTTATG TGTGGGAGGG CTAAGCACTT CGTGGCCGAG GAGCAGGACT
  601 GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA
  661 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT
  721 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGNTTA CAAATAAAGC AATAGCATCA
  781 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCNAACTCA
  841 TCAATGTATC TTATCATGTC TGTATACNGT CGANCTCTAN CTAGANCTNG NCGTAATCAT
  901 GGTCATAGCT GTTTCCTGNG TGAAATTGTT ATCCGCTCNC AANNCCNCAC NNCATACNAN
961 CNNGAAGCAT AAAGNGTAAN NNNNGGGNNN NNNNNNGANT GANCTAANNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles