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pEF6-FLAG-human TFB1M (#RDB17344)

Expression vector of human TFB1M tagged with FLAG at N-terminus.

Clone info. Expression vector of human transcription factor B1, mitochondrial (TFB1M) tagged with FLAG at N-terminus.
Vector backbone pEF6/myc-His (plasmid)
Selectable markers Amp^r (E. coli), BSD (mammalian cells).
Gene/insert name human TFB1M cDNA
Depositor|Developer Maruyama, Takashi |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Tatsushi Muta is requested.
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RDB17344 pEF6-FLAG-human TFB1M DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF6-FLAG-human TFB1M was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17344).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17344_A9Etp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1alpha pro, FLAG, insert5'
Sequence file: RDB17344_A9Eta.seq check
>D05962A9_A9Et_1_EF1a-C_F_A04_01_ABI24.ab1
    1 NNNNNNNNNN NTTTTGAGTT TGGNNCTTGG TTNNTTCTCN AGCCTCAGAC AGTGGTTCAA
   61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGAATTAGCT TGGTACTAAT ACGACTCACT
  121 ATAGGGAGAC CCAAGCTGGC TAGGTAAGCT TGGTACCGAG CTCGGATCGC CACCATGGAC
  181 TACAAGGACG ACGATGACAA GGGATCCACC ATGGCTGCCT CCGGAAAACT CAGCACTTGC
  241 CGTCTCCCTC CGTTGCCCAC GATTCGAGAA ATCATTAAGT TGTTAAGACT GCAAGCAGCG
  301 AAGCAGCTAT CACAGAATTT CCTCCTGGAC TTGAGGCTGA CAGATAAGAT TGTAAGGAAA
  361 GCTGGCAATC TGACAAATGC TTATGTTTAC GAAGTGGGCC CTGGGCCAGG GGGAATCACA
  421 AGATCTATTC TTAATGCCGA CGTCGCTGAA CTTCTGGTGG TTGAAAAGGA CACTCGATTT
  481 ATTCCTGGAT TACAGATGCT TTCTGATGCA GCACCTGGGA AACTGAGAAT TGTTCATGGA
  541 GATGTCTTGA CATTTAAGGT AGAAAAGGCT TTTTCAGAAA GTCTTAAAAG ACCCTGGGAA
  601 GATGATCCTC CAAATGTACA TATTATTGGA AATCTGCCTT TTAGTGTTTC AACTCCACTG
  661 ATTATCAAGT GGCTTGAAAA TATTTCCTGT AGAGATGGAN CTTTTGTTTA TGGCAGAACT
  721 CAGATGACTT TGACTTTTCN AAAGGAAGTG GCAGANAGAN TTGCAGCCAA TACAGGAAGC
  781 AAACAGCGTA NTCGCCTCTC TGTTATGGCT CANTACCTCT GCAATGTTCG ACACATCNTN
  841 ACAATTCCNG GANAAGCTTT TGTNCNNNAA NNNGANGTGG ANGTGGGCGN GGTGCANTTC
  901 ACNCCCTTNN NACAGCCCNA GANAGANCAG CNNNTNNNGC NNNNNGAAAA AGNNGNNCAN
961 NNNGTANTTC ANNNNNNNNN N
//
Primer: BGH_rev2 (Pr0606)
Region: BGH p(A), insert3'
Sequence file: RDB17344_A9Etb.seq check
>D05962A9_A9Et_1_BGH_rev2_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGNG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACTCAATG GTGATGGTGA TGATGACCGG TACGCGTAGA
  121 ATCGAGACCG AGGAGAGGGT TAGGGATAGG CTTACCTTCG AAGGGCCCTC TAGACTCGAG
  181 CGGCCGCCAC TGTGCTGGAT ATCTGCAGAA TTCCTAGAGT CTGTAATTCT CTGCGTCATC
  241 CTCTTCTTTT TCTTCATTTT TGCTTTTTCT TCGCTTGAGT TCTTCTCTGA AATTATATGC
  301 AAAGAGTTGT GGGTCTTCAT CACACATTTT TCTGTATACA TCACAGAGGC TCTTAAAGTG
  361 TGAGATGGAG AGCTGGCGGG GCCGAAGAGT AGGGTCTATG TCTGCCAACT CTAACAGCCT
  421 GCCCGTGCTT TCCAAGCGCT GCGCTTCAGG GAATAACATT CTGAGCCCTC GATGGCAGTA
  481 TTTCCTTCGG AACTGAAATA CATTCTGAAC CACTTTTTCC ACCAGCTTGA ATGGCTGCTC
  541 TATCTTGGGC TGTATCAAGG GAGTGAAGTG CACCACGCCC ACGTCCACCT CTGGTTTGGG
  601 GACAAAAGCT TGTCCTGGAA TTGTAAAGAT GTGTCGAACA TTGCAGAGGT ACTGAGCCAT
  661 AACAGAGAGG CGACTACGCT GTTTGCTTCC TGTATTGNCT GCAAGTCTCT CTGCCACTTC
  721 CTTTTGAAAA GTCAAAGTCA TCTGANTTCT GCCATAAACA AAAGGTCCAT CTCTACAGGA
  781 AATATTTTCN AGCCACTTGA TAATCAGTGG ANTTGAAACA CTAAAAGGCA GATTTCCNAT
  841 AATATGTACA TTTGGAGGNN CATCTTCCCN NGNNCNTTNA AGANTTTCTG AAAAAGCNNT
  901 TTCTANCTTA AATGTCNAGA CNTCTNNNTN AANNATTNNC ANTTTCNNNG GNNCTGCATC
961 NNAAANNATC NNNNANTCCN NNNNNNANNN NNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40pro_ori, BSD
Sequence file: RDB17344_A9Etc.seq check
>D05962A9_A9Et_1_SV40pro_F_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNCNNANN NNTGNNGNNG NTTTTTTGGA NGNCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA
  121 TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG
  181 CCTTTGTCTC AAGAAGAATC CACCCTCATT GAAAGAGCAA CGGCTACAAT CAACAGCATC
  241 CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT CTAGCGACGG CCGCATCTTC
  301 ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG CAGAACTCGT GGTGCTGGGC
  361 ACTGCTGCTG CTGCGGCAGC TGGCAACCTG ACTTGTATCG TCGCGATCGG AAATGAGAAC
  421 AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC TTCTCGATCT GCATCCTGGG
  481 ATCAAAGCCA TAGTGAAGGA CAGTGATGGA CAGCCGACGG CAGTTGGGAT TCGTGAATTG
  541 CTGCCCTCTG GTTATGTGTG GGAGGGCTAA GCACTTCGTG GCCGANGAGC AGGACTGACA
  601 CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGNTGGGCT TCGGAATCGT
  661 TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG ANTTCTTCGC
  721 CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACNAA TAAAGCAATA GCATCACAAA
  781 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCNA
  841 TGTATCTTAT CATGTCTGTA TACNGTCGNN CTCTANCTAG ANCTTGGCGT AATCATGGNC
  901 ATAGCTGTTN CCTGNGTGAA ATTGNTANCC GCTCNCAATT CCNCACNNCN TACNANCNNG
  961 AAGCATAANG TGNAAAGNCT GGGNNNNNNN NNGNNTGANC TAANNNNNNN NNANNNNNNN
1021 NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles