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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF6-FLAG-human TFB1M

Expression vector of human TFB1M tagged with FLAG at N-terminus.

Catalog number RDB17344
Resource name pEF6-FLAG-human TFB1M
Clone info. Expression vector of human transcription factor B1, mitochondrial (TFB1M) tagged with FLAG at N-terminus.
Vector backbone pEF6/myc-His (plasmid)
Selectable markers Amp^r (E. coli), BSD (mammalian cells).
Gene/insert name human TFB1M cDNA
Depositor|Developer Maruyama, Takashi |
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External Database
human TFB1M

          Reference sequence
            
           

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          RDB17344 pEF6-FLAG-human TFB1M DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEF6-FLAG-human TFB1M was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17344).

          Reference section:

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17344_A9Etp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F (Pr0168)
          Region: EF1alpha pro, FLAG, insert5'
          Sequence file: RDB17344_A9Eta.seq check
          >D05962A9_A9Et_1_EF1a-C_F_A04_01_ABI24.ab1
              1 NNNNNNNNNN NTTTTGAGTT TGGNNCTTGG TTNNTTCTCN AGCCTCAGAC AGTGGTTCAA
             61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGAATTAGCT TGGTACTAAT ACGACTCACT
            121 ATAGGGAGAC CCAAGCTGGC TAGGTAAGCT TGGTACCGAG CTCGGATCGC CACCATGGAC
            181 TACAAGGACG ACGATGACAA GGGATCCACC ATGGCTGCCT CCGGAAAACT CAGCACTTGC
            241 CGTCTCCCTC CGTTGCCCAC GATTCGAGAA ATCATTAAGT TGTTAAGACT GCAAGCAGCG
            301 AAGCAGCTAT CACAGAATTT CCTCCTGGAC TTGAGGCTGA CAGATAAGAT TGTAAGGAAA
            361 GCTGGCAATC TGACAAATGC TTATGTTTAC GAAGTGGGCC CTGGGCCAGG GGGAATCACA
            421 AGATCTATTC TTAATGCCGA CGTCGCTGAA CTTCTGGTGG TTGAAAAGGA CACTCGATTT
            481 ATTCCTGGAT TACAGATGCT TTCTGATGCA GCACCTGGGA AACTGAGAAT TGTTCATGGA
            541 GATGTCTTGA CATTTAAGGT AGAAAAGGCT TTTTCAGAAA GTCTTAAAAG ACCCTGGGAA
            601 GATGATCCTC CAAATGTACA TATTATTGGA AATCTGCCTT TTAGTGTTTC AACTCCACTG
            661 ATTATCAAGT GGCTTGAAAA TATTTCCTGT AGAGATGGAN CTTTTGTTTA TGGCAGAACT
            721 CAGATGACTT TGACTTTTCN AAAGGAAGTG GCAGANAGAN TTGCAGCCAA TACAGGAAGC
            781 AAACAGCGTA NTCGCCTCTC TGTTATGGCT CANTACCTCT GCAATGTTCG ACACATCNTN
            841 ACAATTCCNG GANAAGCTTT TGTNCNNNAA NNNGANGTGG ANGTGGGCGN GGTGCANTTC
            901 ACNCCCTTNN NACAGCCCNA GANAGANCAG CNNNTNNNGC NNNNNGAAAA AGNNGNNCAN
          961 NNNGTANTTC ANNNNNNNNN N
          //
          Primer: BGH_rev2 (Pr0606)
          Region: BGH p(A), insert3'
          Sequence file: RDB17344_A9Etb.seq check
          >D05962A9_A9Et_1_BGH_rev2_A11_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGGNG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGGGTTT AAACTCAATG GTGATGGTGA TGATGACCGG TACGCGTAGA
            121 ATCGAGACCG AGGAGAGGGT TAGGGATAGG CTTACCTTCG AAGGGCCCTC TAGACTCGAG
            181 CGGCCGCCAC TGTGCTGGAT ATCTGCAGAA TTCCTAGAGT CTGTAATTCT CTGCGTCATC
            241 CTCTTCTTTT TCTTCATTTT TGCTTTTTCT TCGCTTGAGT TCTTCTCTGA AATTATATGC
            301 AAAGAGTTGT GGGTCTTCAT CACACATTTT TCTGTATACA TCACAGAGGC TCTTAAAGTG
            361 TGAGATGGAG AGCTGGCGGG GCCGAAGAGT AGGGTCTATG TCTGCCAACT CTAACAGCCT
            421 GCCCGTGCTT TCCAAGCGCT GCGCTTCAGG GAATAACATT CTGAGCCCTC GATGGCAGTA
            481 TTTCCTTCGG AACTGAAATA CATTCTGAAC CACTTTTTCC ACCAGCTTGA ATGGCTGCTC
            541 TATCTTGGGC TGTATCAAGG GAGTGAAGTG CACCACGCCC ACGTCCACCT CTGGTTTGGG
            601 GACAAAAGCT TGTCCTGGAA TTGTAAAGAT GTGTCGAACA TTGCAGAGGT ACTGAGCCAT
            661 AACAGAGAGG CGACTACGCT GTTTGCTTCC TGTATTGNCT GCAAGTCTCT CTGCCACTTC
            721 CTTTTGAAAA GTCAAAGTCA TCTGANTTCT GCCATAAACA AAAGGTCCAT CTCTACAGGA
            781 AATATTTTCN AGCCACTTGA TAATCAGTGG ANTTGAAACA CTAAAAGGCA GATTTCCNAT
            841 AATATGTACA TTTGGAGGNN CATCTTCCCN NGNNCNTTNA AGANTTTCTG AAAAAGCNNT
            901 TTCTANCTTA AATGTCNAGA CNTCTNNNTN AANNATTNNC ANTTTCNNNG GNNCTGCATC
          961 NNAAANNATC NNNNANTCCN NNNNNNANNN NNNNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: SV40pro_ori, BSD
          Sequence file: RDB17344_A9Etc.seq check
          >D05962A9_A9Et_1_SV40pro_F_C04_07_ABI24.ab1
              1 NNNNNNNNNN NNNNCNNANN NNTGNNGNNG NTTTTTTGGA NGNCTAGGCT TTTGCAAAAA
             61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA
            121 TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG
            181 CCTTTGTCTC AAGAAGAATC CACCCTCATT GAAAGAGCAA CGGCTACAAT CAACAGCATC
            241 CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT CTAGCGACGG CCGCATCTTC
            301 ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG CAGAACTCGT GGTGCTGGGC
            361 ACTGCTGCTG CTGCGGCAGC TGGCAACCTG ACTTGTATCG TCGCGATCGG AAATGAGAAC
            421 AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC TTCTCGATCT GCATCCTGGG
            481 ATCAAAGCCA TAGTGAAGGA CAGTGATGGA CAGCCGACGG CAGTTGGGAT TCGTGAATTG
            541 CTGCCCTCTG GTTATGTGTG GGAGGGCTAA GCACTTCGTG GCCGANGAGC AGGACTGACA
            601 CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGNTGGGCT TCGGAATCGT
            661 TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG ANTTCTTCGC
            721 CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACNAA TAAAGCAATA GCATCACAAA
            781 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCNA
            841 TGTATCTTAT CATGTCTGTA TACNGTCGNN CTCTANCTAG ANCTTGGCGT AATCATGGNC
            901 ATAGCTGTTN CCTGNGTGAA ATTGNTANCC GCTCNCAATT CCNCACNNCN TACNANCNNG
            961 AAGCATAANG TGNAAAGNCT GGGNNNNNNN NNGNNTGANC TAANNNNNNN NNANNNNNNN
          1021 NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl