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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pENTR4XE_BAZ1B (#RDB17335)

Entry vector of human BAZ1B without stop codon.

Clone info. Entry vector of human bromodomain adjacent to zinc finger domain 1B (BAZ1B) without stop codon.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pENTR4 (plasmid)
Selectable markers Kan^r
Gene/insert name human BAZ1B cDNA
Depositor|Developer Ohta, Shinya |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ohta S. et al. Mol. Cell Proteomics. 18(2), 169-181, 2019). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ohta S. et al. Mol. Cell Proteomics. 18(2), 169-181, 2019)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17335 pENTR4XE_BAZ1B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pENTR4XE_BAZ1B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17335).

Reference section:

Ohta, S., Taniguchi, T., Sato, N., Hamada, M., Taniguchi, H., Rappsilber, J., Quantitative Proteomics of the Mitotic Chromosome Scaffold Reveals the Association of BAZ1B with Chromosomal Axes. Mol. Cell. Proteomics 18 (2): 169-181 (2019). PMID 30266865. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17335_A9Ehp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: rrnBT1ter_F ()
Region: rrnB T1T2 terminator, attL1, insert 5'
Sequence file: RDB17335_A9Eha.seq check
>D06261A3_A9Eh_1_rrnBT1ter_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNGTNNNG ANCANNGCCC NGNNGGTGGC GGGCAGGANG CCCGCCATAA
   61 ACTGCCAGGC ATCAAACTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA
  121 CAAACTCTTC CTGTTAGTTA GTTACTTAAG CTCGGGCCCC AAATAATGAT TTTATTTTGA
  181 CTGATAGTGA CCTGTTCGTT GCAACAAATT GATAAGCAAT GCTTTTTTAT AATGCCAACT
  241 TTGTACAAAA AAGCAGGCTC CACCATGGGA ACCATGGCGC CGCTCCTGGG CCGCAAGCCC
  301 TTCCCGCTGG TGAAGCCGTT GCCCGGAGAG GAGCCGCTCT TCACCATCCC GCACACTCAG
  361 GAGGCCTTCC GCACCCGGGA AGAGTATGAA GCCCGCTTGG AAAGGTACAG TGAGCGCATT
  421 TGGACGTGCA AGAGTACTGG AAGCAGTCAG CTAACACACA AGGAAGCCTG GGAGGAAGAA
  481 CAGGAAGTTG CTGAGCTTTT GAAGGAGGAG TTTCCTGCCT GGTATGAGAA GCTTGTTCTG
  541 GAAATGGTTC ACCATAACAC AGCCTCCTTA GAGAAGTTAG TAGATACTGC TTGGTTGGAG
  601 ATCATGACCA AATATGCTGT GGGAGAAGAG TGTGACTTCG AGGTTGGGAA GGAGAAAATG
  661 CTCAAGGTGA AGATTGTGAA GATTCATCCT TTGGAGAAAG TGGATGANNA GGCCACTGAN
  721 AAGAAATCTG ATGGTGCCTG TGANTCTCCA TCAAGTGACA AAGAGAACTC CAGTCAGATT
  781 GCTCANGACC ATCANAANAA NGANACAGTT GTNNAAGANG ANGANNGAAN GANAGAGAGT
  841 ATTNNTGANN NANCACGTAN ATNGCCNCGA AAACNTNCCT NNTTCATTAA AAAAAANGAN
  901 AAAGGANNGG GCTCCTCCAA ATTTCTGNCT CNNNAATATG ATGTGAAACN NNNAAAATGA
961 AGATNNNNNN NNNANNNNNN NNNN
//
Primer: KanR-N_R ()
Region: attL2, insert 3'
Sequence file: RDB17335_A9Ehb.seq check
>D06261A3_A9Eh_1_KanR-N_R_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN NNNTNNNCAT CNNTGTTGGN ATTTAATCGC GGCCTCGACG
   61 TTTCCCGTTG AATATGGCTC ATAACACCCC TTGTATTACT GTTTATGTAA GCAGACAGTT
  121 TTATTGTTCA TGATGATATA TTTTTATCTT GTGCAATGTA ACATCAGAGA TTTTGAGACA
  181 CGGGCCAGAG CTGCAGCTGG ATGGCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT
  241 TCGTTGCAAC AAATTGATAA GCAATGCTTT CTTATAATGC CAACTTTGTA CAAGAAAGCT
  301 GGGTCTAGAT GCTTCTTCTG TCTTCGTCCC CTGGACTGTC CAACGGCCTC TGGCTCACTG
  361 TCCCCTTCAT CTTCAGCAAG CCTATCAGGA AACTTCTTGC GCTTCCTGCG GACATATGGG
  421 TGGCCAGGAA GGTGTTTATG CAACAGAGCC ACTAGACACT GTTCTGTCTT CACCATGCAG
  481 CTTAGCACAT GGCTGCCACG GCAGTTGTAA ACCTCAGCAT TGGTAAACAC TTGCTTCATG
  541 TCAGTAAGAA ACTCCTGCAC AGAGCGGTAG CTCCCACAGG AACATTTGTT CTGCACTGTC
  601 TGAAAGTCCA TGGGGTGCGT GATCACATCA TAGTAGTCCT CGGCCTCATC TCTGGTCACA
  661 GGCTCCCTGA AGGGCCAGCT GAAGCGGTAC TTCACGATCT TGTGGAGGAT CTCTTCACAC
  721 TTCTGCAGCT CCAGGCTTTG CCTCCGGGAG CTCCGCTTGG TCTGAAGCAC CAGCTCATCC
  781 ACCTCAGCAT CATCCACANG TGGTGCCTTG GGCTGANNNC TCCTNGTAGA GTGTGGCTTC
  841 TTACCCGGGC GCCNGNCTGA CCTTGCTGCN NGGGGGANGA NGCTGNNCTN GNCCCGGATG
  901 GNCTTTCGAG GNCTCNANCN CAAACCANCC ACCTCANAAT CTTCNTNCNC CTNNNNCNNN
961 NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ohta, S., Quantitative Proteomics of the Mitotic Chromosome Scaffold Reveals the Association of BAZ1B with Chromosomal Axes. Mol. Cell. Proteomics 18 (2): 169-181 (2019). PMID 30266865. [link to RRC of NBRP]