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pENTR4XE_BAZ1B

Entry vector of human BAZ1B without stop codon.

Catalog number RDB17335
Resource name pENTR4XE_BAZ1B
Clone info. Entry vector of human bromodomain adjacent to zinc finger domain 1B (BAZ1B) without stop codon.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pENTR4 (plasmid)
Selectable markers Kan^r
Gene/insert name human BAZ1B cDNA
Depositor Ohta, Shinya |
 
Other clones in our bank human BAZ1B (NCBI Gene 9031) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ohta S. et al. Mol. Cell Proteomics. 18(2), 169-181, 2019). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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RDB17335 pENTR4XE_BAZ1B DNA solution

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References and tips

Electronic file

Original reference

original Ohta, S., Quantitative Proteomics of the Mitotic Chromosome Scaffold Reveals the Association of BAZ1B with Chromosomal Axes. Mol. Cell. Proteomics 18 (2): 169-181 (2019). PMID 30266865.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB17335_A9Ehp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: rrnB T1T2 terminator, attL1, insert 5' | Primer: rrnBT1ter_F | Sequence: RDB17335_A9Eha.seq
>D06261A3_A9Eh_1_rrnBT1ter_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNGTNNNG ANCANNGCCC NGNNGGTGGC GGGCAGGANG CCCGCCATAA
   61 ACTGCCAGGC ATCAAACTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA
  121 CAAACTCTTC CTGTTAGTTA GTTACTTAAG CTCGGGCCCC AAATAATGAT TTTATTTTGA
  181 CTGATAGTGA CCTGTTCGTT GCAACAAATT GATAAGCAAT GCTTTTTTAT AATGCCAACT
  241 TTGTACAAAA AAGCAGGCTC CACCATGGGA ACCATGGCGC CGCTCCTGGG CCGCAAGCCC
  301 TTCCCGCTGG TGAAGCCGTT GCCCGGAGAG GAGCCGCTCT TCACCATCCC GCACACTCAG
  361 GAGGCCTTCC GCACCCGGGA AGAGTATGAA GCCCGCTTGG AAAGGTACAG TGAGCGCATT
  421 TGGACGTGCA AGAGTACTGG AAGCAGTCAG CTAACACACA AGGAAGCCTG GGAGGAAGAA
  481 CAGGAAGTTG CTGAGCTTTT GAAGGAGGAG TTTCCTGCCT GGTATGAGAA GCTTGTTCTG
  541 GAAATGGTTC ACCATAACAC AGCCTCCTTA GAGAAGTTAG TAGATACTGC TTGGTTGGAG
  601 ATCATGACCA AATATGCTGT GGGAGAAGAG TGTGACTTCG AGGTTGGGAA GGAGAAAATG
  661 CTCAAGGTGA AGATTGTGAA GATTCATCCT TTGGAGAAAG TGGATGANNA GGCCACTGAN
  721 AAGAAATCTG ATGGTGCCTG TGANTCTCCA TCAAGTGACA AAGAGAACTC CAGTCAGATT
  781 GCTCANGACC ATCANAANAA NGANACAGTT GTNNAAGANG ANGANNGAAN GANAGAGAGT
  841 ATTNNTGANN NANCACGTAN ATNGCCNCGA AAACNTNCCT NNTTCATTAA AAAAAANGAN
  901 AAAGGANNGG GCTCCTCCAA ATTTCTGNCT CNNNAATATG ATGTGAAACN NNNAAAATGA
  961 AGATNNNNNN NNNANNNNNN NNNNNNCANA NNNNCNTNNN NNNNNNNNNN NNNNNNNNNN
 1021 NNNANNNNNN NNNNNNNNNN NNNNNNTNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNN
//
Region: attL2, insert 3' | Primer: KanR-N_R | Sequence: RDB17335_A9Ehb.seq
>D06261A3_A9Eh_1_KanR-N_R_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN NNNTNNNCAT CNNTGTTGGN ATTTAATCGC GGCCTCGACG
   61 TTTCCCGTTG AATATGGCTC ATAACACCCC TTGTATTACT GTTTATGTAA GCAGACAGTT
  121 TTATTGTTCA TGATGATATA TTTTTATCTT GTGCAATGTA ACATCAGAGA TTTTGAGACA
  181 CGGGCCAGAG CTGCAGCTGG ATGGCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT
  241 TCGTTGCAAC AAATTGATAA GCAATGCTTT CTTATAATGC CAACTTTGTA CAAGAAAGCT
  301 GGGTCTAGAT GCTTCTTCTG TCTTCGTCCC CTGGACTGTC CAACGGCCTC TGGCTCACTG
  361 TCCCCTTCAT CTTCAGCAAG CCTATCAGGA AACTTCTTGC GCTTCCTGCG GACATATGGG
  421 TGGCCAGGAA GGTGTTTATG CAACAGAGCC ACTAGACACT GTTCTGTCTT CACCATGCAG
  481 CTTAGCACAT GGCTGCCACG GCAGTTGTAA ACCTCAGCAT TGGTAAACAC TTGCTTCATG
  541 TCAGTAAGAA ACTCCTGCAC AGAGCGGTAG CTCCCACAGG AACATTTGTT CTGCACTGTC
  601 TGAAAGTCCA TGGGGTGCGT GATCACATCA TAGTAGTCCT CGGCCTCATC TCTGGTCACA
  661 GGCTCCCTGA AGGGCCAGCT GAAGCGGTAC TTCACGATCT TGTGGAGGAT CTCTTCACAC
  721 TTCTGCAGCT CCAGGCTTTG CCTCCGGGAG CTCCGCTTGG TCTGAAGCAC CAGCTCATCC
  781 ACCTCAGCAT CATCCACANG TGGTGCCTTG GGCTGANNNC TCCTNGTAGA GTGTGGCTTC
  841 TTACCCGGGC GCCNGNCTGA CCTTGCTGCN NGGGGGANGA NGCTGNNCTN GNCCCGGATG
  901 GNCTTTCGAG GNCTCNANCN CAAACCANCC ACCTCANAAT CTTCNTNCNC CTNNNNCNNN
  961 NNNNNNNNNN NNNNNNNNNN CATCANNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1021 NNNNNNNNNN NNTNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGNNNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ohta, S., Quantitative Proteomics of the Mitotic Chromosome Scaffold Reveals the Association of BAZ1B with Chromosomal Axes. Mol. Cell. Proteomics 18 (2): 169-181 (2019). PMID 30266865.

2019.08.19

GNP_filter3_RDBDEP_html_190816.pl