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pDEST131NGFP_BAZ1B (#RDB17334)

Expression vector of human BAZ1B tagged with GFP at N-terminus.

Clone info. Expression vector of human bromodomain adjacent to zinc finger domain 1B (BAZ1B) tagged with GFP at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pDEST131NGFP (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human BAZ1B cDNA
Depositor|Developer Ohta, Shinya |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ohta S. et al. Mol. Cell Proteomics. 18(2), 169-181, 2019). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ohta S. et al. Mol. Cell Proteomics. 18(2), 169-181, 2019)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17334 pDEST131NGFP_BAZ1B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST131NGFP_BAZ1B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17334).

Reference section:

Ohta, S., Taniguchi, T., Sato, N., Hamada, M., Taniguchi, H., Rappsilber, J., Quantitative Proteomics of the Mitotic Chromosome Scaffold Reveals the Association of BAZ1B with Chromosomal Axes. Mol. Cell. Proteomics 18 (2): 169-181 (2019). PMID 30266865. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17334_A9Ehp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F ()
Region: EGFP 3', attB1, insert 5'
Sequence file: RDB17334_A9Eha.seq check
>D06261A2_A9Eh_3_mVenus-M_F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNGGCNTC NNGGNGANTT CNNGANCCGC CNCAACATCG AGGNNGGCAG
   61 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
  121 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
  181 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
  241 GCTGTACAAG GGTACCACAA GTTTGTACAA AAAAGCAGGC TCCACCATGG GAACCATGGC
  301 GCCGCTCCTG GGCCGCAAGC CCTTCCCGCT GGTGAAGCCG TTGCCCGGAG AGGAGCCGCT
  361 CTTCACCATC CCGCACACTC AGGAGGCCTT CCGCACCCGG GAAGAGTATG AAGCCCGCTT
  421 GGAAAGGTAC AGTGAGCGCA TTTGGACGTG CAAGAGTACT GGAAGCAGTC AGCTAACACA
  481 CAAGGAAGCC TGGGAGGAAG AACAGGAAGT TGCTGAGCTT TTGAAGGAGG AGTTTCCTGC
  541 CTGGTATGAG AAGCTTGTTC TGGAAATGGT TCACCATAAC ACAGCCTCCT TAGAGAAGTT
  601 AGTAGATACT GCTTGGTTGG AGATCATGAC CAAATATGCT GTGGGAGAAG AGTGTGACTT
  661 CGAGGTTGGG AAGGAGAAAA TGCTCAAGGT GAAGATTGTG AAGATTCATC CTTTGGAGAA
  721 AGTGGATGAA GAGGCCACTG AGAAGAAATC TGATGGTGCC TGTGANTCTC CNTCAAGTGA
  781 CANAGAGAAC TCCAGTCAGA TTGCTCANGA NCATCAGAAG ANNGAGANAG TTGTGAAAGA
  841 NGATGANNGA AGGANAGAGA GTATTAATGA CAGAGCACGT ANNTCGCCNC GAAANCTTCC
  901 TACTTNNTTN AAAAAAAGGA GAANNGAAAT GGGNCTCNNN NNAAATTNNT GNCNNCNCAA
961 NNNTGNNGTN NAACTNNNAA ANNNNNNNNN N
//
Primer: BGH_rev2 ()
Region: BGH poly(A) signal, attB2, insert 3'
Sequence file: RDB17334_A9Ehb.seq check
>D06261A2_A9Eh_3_BGH_rev2_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGGG GNNGGGCNNA NAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTA CTTTAGACCA CTTTGTACAA
  121 GAAAGCTGGG TCTAGATGCT TCTTCTGTCT TCGTCCCCTG GACTGTCCAA CGGCCTCTGG
  181 CTCACTGTCC CCTTCATCTT CAGCAAGCCT ATCAGGAAAC TTCTTGCGCT TCCTGCGGAC
  241 ATATGGGTGG CCAGGAAGGT GTTTATGCAA CAGAGCCACT AGACACTGTT CTGTCTTCAC
  301 CATGCAGCTT AGCACATGGC TGCCACGGCA GTTGTAAACC TCAGCATTGG TAAACACTTG
  361 CTTCATGTCA GTAAGAAACT CCTGCACAGA GCGGTAGCTC CCACAGGAAC ATTTGTTCTG
  421 CACTGTCTGA AAGTCCATGG GGTGCGTGAT CACATCATAG TAGTCCTCGG CCTCATCTCT
  481 GGTCACAGGC TCCCTGAAGG GCCAGCTGAA GCGGTACTTC ACGATCTTGT GGAGGATCTC
  541 TTCACACTTC TGCAGCTCCA GGCTTTGCCT CCGGGAGCTC CGCTTGGTCT GAAGCACCAG
  601 CTCATCCACC TCAGCATCAT CCACAGGTGG TGCCTTGGGC TGAGACCTCC TGGTAGAGTG
  661 TGGCTTCTTA CCCGGGCGCC GGCCTGACCT TGCTGCAGGG GGGANGACGC TGTGCTTGCC
  721 CCGGATGGTC TTTCGAGGTC TCAATCGCAA ACCAGCCACC TCATAATCTT CTTCCTCCTC
  781 CTCCTCTTNN NNNTCCTCCT CCTCTTCATC ACTCTCATCA TCTTCACTGT CCTCANAAGC
  841 ANANTCTTCA NTATAGTTCC TGCCACGGGN NTTGCGCCNG GCANTANCGG GGCTGGCAAG
  901 CTGGGNACTG CNACTCACCA TCNNGNTACN TCATANANGG NCNNNCNCAN ANAAAACNNN
  961 NGNANNNNTT NNTTANNNTC NTCANANNNG NTCAANTNNN NNNNCNNNNN CNTTNNNTNN
1021 NNNNANNNTT NNNNNNNNNN
//
Primer: CMV-Forward ()
Region: CMV promoter, EGFP 5'
Sequence file: RDB17334_A9Ehc.seq check
>D06261A2_A9Eh_3_CMV_Forward_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNNN NGCNNTGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACG ATGGTGAGCA
  181 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  241 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  301 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  361 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  421 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  481 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  541 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  601 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  661 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  721 AGCAGAACAC CCCCATCGGC GACNGCCCCG TGCTGCTGCC CGACAACCAC TACCTGANCA
  781 CCCAGTCCGC CNTGANCANA GNNCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGANT
  841 TCGNGACCGC CGCCNGGNTC ACTCTCGGCA TGGNNGANCT GNANNNNNNT ANCACNNGTT
  901 TGTANNAAAA ANCNNNNTCC NCCNNNGGNA NCCANGGNNC NNCNNCNGGG NCNNNNAGCC
961 NNTNCCCNCN NNNNNNNNCN NNTNNNCNNG NANNNGNNNN CNNNNNNNNN N
//
Primer: SV40polyA-R2 ()
Region: SV40 poly(A) signal, NeoR/KanR
Sequence file: RDB17334_A9Ehd.seq check
>D06261A2_A9Eh_3_SV40polyA-R2_H01_22_ABI24.ab1
    1 NNNNNNNNNC NNNNNNANNN NNGTTGGGGT GGGCGAANAA CTCCNGCATG AGNTCCCCGC
   61 GCTGGANGNN CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA
  121 AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT
  181 CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG
  241 CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG
  301 CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGGTCCG CCACACCCAG
  361 CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA
  421 GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCGCGCCT TGAGCCTGGC
  481 GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG
  541 ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG
  601 GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT
  661 CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG
  721 CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGN
  781 GGCCAGCCAC GATAGCCGCG CTGCCTCGTC CTGCAGTTCA TTCNNGGCAC CGGANANGTC
  841 GGTCTTGANA AAAAGAANCG GGCGCCCCTG CGCTNANAGC CGGAACACGG CNGCNTCANA
  901 NCAGCCGANT GTCNGNTGTG CCCAGNCATA NCCNAANNNC CNCTNNNCCN NNNNNNNCNG
  961 NANNANCNNN NNNGNNNTNN NNNNTNNNTN NNNNNNNGNN NANNNNNNCN NNNNNCNNNN
1021 NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ohta, S., Quantitative Proteomics of the Mitotic Chromosome Scaffold Reveals the Association of BAZ1B with Chromosomal Axes. Mol. Cell. Proteomics 18 (2): 169-181 (2019). PMID 30266865. [link to RRC of NBRP]