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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST131NGFP_BAZ1A (#RDB17333)

Expression vector of human BAZ1A tagged with GFP at N-terminus.

Clone info. Expression vector of human bromodomain adjacent to zinc finger domain 1A (BAZ1A) tagged with GFP at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pDEST131NGFP (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human BAZ1A cDNA
Depositor|Developer Ohta, Shinya |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ohta S. et al. Mol. Cell Proteomics. 18(2), 169-181, 2019). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ohta S. et al. Mol. Cell Proteomics. 18(2), 169-181, 2019)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
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Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

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RDB17333 pDEST131NGFP_BAZ1A DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST131NGFP_BAZ1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17333).

Reference section:

Ohta, S., Taniguchi, T., Sato, N., Hamada, M., Taniguchi, H., Rappsilber, J., Quantitative Proteomics of the Mitotic Chromosome Scaffold Reveals the Association of BAZ1B with Chromosomal Axes. Mol. Cell. Proteomics 18 (2): 169-181 (2019). PMID 30266865. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17333_A9Ehp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F ()
Region: EGFP 3', attB1, insert 5'
Sequence file: RDB17333_A9Eha.seq check
>D06261A1_A9Eh_1_mVenus-M_F_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNTCNNGGNG ANNTTCANGA NCCGCCACNA NCATCGAGGN
   61 CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT
  121 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA
  181 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT
  241 GGACGAGCTG TACAAGGGTA CCACAAGTTT GTACAAAAAA GCAGGCTTCG AAGGAGATAG
  301 AACCATGCCG CTGCTACACC GAAAGCCGTT TGTGAGACAG AAGCCGCCCG CGGACCTGCG
  361 GCCCGACGAG GAAGTTTTCT ACTGTAAAGT CACCAACGAG ATCTTCCGCC ACTACGATGA
  421 CTTTTTTGAA CGAACCATTC TGTGCAACAG CCTTGTGTGG AGTTGTGCTG TGACGGGTAG
  481 ACCTGGACTG ACGTATCAGG AAGCACTTGA GTCAGAAAAA AAAGCAAGAC AGAATCTTCA
  541 GAGTTTTCCA GAACCACTAA TTATTCCAGT TTTATACTTG ACCAGCCTTA CCCATCGTTC
  601 GCGCTTACAT GAAATTTGTG ATGATATCTT TGCATATGTC AAGGATCGAT ATTTTGTCGA
  661 AGAAACTGTG GAAGTCATTA GGAACAATGG TGCAAGGTTG CAGTGTANGA TTTTGGAAGT
  721 CCTCCCTCCA TCACATCAAA ATGGTTTTGC TAATGGACAT GTNNACAGTG TGGATGGAGA
  781 AACTATTATC ATCAGTGATA GTGATGATTC AGAAACACAN AGCTGTTCTT TTCAAANTGG
  841 GAAGAAAAAA GATGCNATTG ATCCCTTACT ATTCANGTAT AAAGTGNNNC CCNCTAAAAA
  901 ANAATTACAT GANTCTGCTA TTGTNNAAGC NACACAAATC ANCNGGANAA AACACCTATT
  961 TTNNNGNGNT AAACNNAAAG CTTTTTTNNG ANNCAACNNN NNNNANNNNN NNGNNNNGNN
1021 NNNNNNNN
//
Primer: BGH_rev2 ()
Region: BGH poly(A) signal, attB2, insert 3'
Sequence file: RDB17333_A9Ehb.seq check
>D06261A1_A9Eh_1_BGH_rev2_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGGG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTAC TTTAGACCAC TTTGTACAAG
  121 AAAGCTGGGT CATAGATTCG TGACTTTTTC GCAGCCGGTG GTGTGCTAAC TTGGTCCACA
  181 TTACTGGGTG TGACGTGGAG TCCAAGCTTT TGAGCCTGAA TATGAAAAAA TGCTTGAAGC
  241 CTAGTTCCAG CTTTTGCTTC ACTTGTGTTA CGAGGGTTGT ATTCAAAGCA GTTCGAAAAC
  301 ATTAACTCAA TGTCATCAAT AAACTCAGAT GCTAATTTAT ATTCACACTT ATTCACTTTT
  361 TCACGAATTA TATTTAAGGC AATGGGCTTT TTGATGATGT CATAGTAGTC TGGGACCTGG
  421 ATTTTAGAAA CAAGTTTCAA AAAAGGCCAG CTGTCATCAT GTCGTACCAA TTCTACAACA
  481 AGTTGTTCAA AAGCAGACAA TTCATGAACT CCTCCCTGTC GGCCAGAACT CCTTCGACCC
  541 AGTGTCACAG GCGATGGCTC TGGAGATTGT CTTTTTCTGC ATCTTCTTTT GGATTCACTC
  601 TCTTGGAGAG AAAGTTTTGA AGCAATATTT ACAGATCTTG ACTGTTCACT TGACTTTGTG
  661 GCAATGACTC TGAAGTTAGG GAAGTTGGGA CTATTTTCTG GTGTATTATT AGCACTTTTC
  721 CTGCCTCTGC GTTTTCTACG ANGANTAAGC AATTCCACAN ATACATCTGC TTGCAGTGNG
  781 CCATGACTGT GGCGAGTANA ACGACTGGCA ATTCTTAAAG ATTTTGTTTC TGTANGANGA
  841 NCGGANTTTA TCTTTCTTAN GCTTCTANNA GTTTTAGAAG AAACAGTTGT TTTGGGTGTG
  901 CTNTGCTGAN TCCTNGNANN GNTATCTTCC NNNTTCTNGT TGNTGGNNNN NANTTGANAA
961 AGNNNNNCTN NNTTTNCNNN NTNNNTTNNN NNNGNNNNNN NNNN
//
Primer: CMV-Forward ()
Region: CMV promoter, EGFP 5'
Sequence file: RDB17333_A9Ehc.seq check
>D06261A1_A9Eh_1_CMV_Forward_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGCNNTGGGC GGTANGCGTG TACGGTGGGN NGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACG ATGGTGAGCA
  181 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  241 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  301 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  361 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  421 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  481 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  541 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  601 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  661 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  721 AGCAGAACAC CCCCATCGGC GACNGNCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
  781 CCCAGTCCGC CCTGANCAAA GANCCCAACG ANAANCGCGA TCACATGGTC CTGCTGGANT
  841 TCGNGANCGC CGCCNGGGAT CACTCTCGGC ANNGANGANC TGTNNNNNGG GTANCANNAG
  901 TTTGTACNAA AAAANCNNNN TNCNAANGAN NNAGNANNNT GNNNNNNCNN CNCCNANNNN
961 NNNTTGNGAN ANANNNNNCN NNNNNNNGNN NNNNNNNN
//
Primer: SV40polyA-R2 ()
Region: SV40 poly(A) signal, NeoR/KanR
Sequence file: RDB17333_A9Ehd.seq check
>D06261A1_A9Eh_1_SV40polyA-R2_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNCNNNNNNN NNGTTGGGGT GGGCGAANAA NTCCNGCATG AGNTCCCCGC
   61 GCTGGAGGAN CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA
  121 AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT
  181 CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG
  241 CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG
  301 CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGGTCCG CCACACCCAG
  361 CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA
  421 GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCGCGCCT TGAGCCTGGC
  481 GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG
  541 ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG
  601 GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT
  661 CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG
  721 CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT
  781 GGCCAGCCAC GATAGCCGCG CTGCCTCGTC CTGCAGTTCA TTCANGGCAC NGGACAGGTC
  841 GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGANAGC CGGAACACGG CGGCATCAGA
  901 NCAGCCNANT GTCNGTTGNG CCCANNCATN NCNNANNNGC CNNNCCNCCC NAGCNGNCGG
961 NNNNNNNGCN NNNNNTCCNN NNNNNTCNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ohta, S., Quantitative Proteomics of the Mitotic Chromosome Scaffold Reveals the Association of BAZ1B with Chromosomal Axes. Mol. Cell. Proteomics 18 (2): 169-181 (2019). PMID 30266865. [link to RRC of NBRP]