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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFLAG-CMV-5a-RPL11

Expression vector of human RPL11 tagged with FLAG at C-terminus.

Catalog number RDB17330
Resource name pFLAG-CMV-5a-RPL11
Clone info. Expression vector of human ribosomal protein L11 (RPL11) tagged with FLAG at C-terminus, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pFLAG-CMV-5a (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name human RPL11 cDNA
Depositor|Developer Fujita, Masatoshi |
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External Database
human RPL11

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kayama K. et al. EMBO Rep. 18, 123-137, 2017).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kayama K. et al. EMBO Rep. 18, 123-137, 2017)。
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          RDB17330 pFLAG-CMV-5a-RPL11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kayama, K., GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO Rep. 18 (1): 123-137 (2017). PMID 27856536.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17330_A9Ehp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, insert, FLAG, hGH pA
          Sequence file: RDB17330_A9Eha.seq check
          >D06321A7_A9Eh_2_CMV_Forward_B06_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTNGN NNCNNATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTCACCATGG CGGATCAAGG TGAAAAGGAG
            121 AACCCCATGC GGGAACTTCG CATCCGCAAA CTCTGTCTCA ACATCTGTGT TGGGGAGAGT
            181 GGAGACAGAC TGACGCGAGC AGCCAAGGTG TTGGAGCAGC TCACAGGGCA GACCCCTGTG
            241 TTTTCCAAAG CTAGATACAC TGTCAGATCC TTTGGCATCC GGAGAAATGA AAAGATTGCT
            301 GTCCACTGCA CAGTTCGAGG GGCCAAGGCA GAAGAAATCT TGGAGAAGGG TCTAAAGGTG
            361 CGGGAGTATG AGTTAAGAAA AAACAACTTC TCAGATACTG GAAACTTTGG TTTTGGGATC
            421 CAGGAACACA TCGATCTGGG TATCAAATAT GACCCAAGCA TTGGTATCTA CGGCCTGGAC
            481 TTCTATGTGG TGCTGGGTAG GCCAGGTTTC AGCATCGCAG ACAAGAAGCG CAGGACAGGC
            541 TGCATTGGGG CCAAACACAG AATCAGCAAA GAGGAGGCCA TGCGCTGGTT CCAGCAGAAG
            601 TATGATGGGA TCATCCTTCC TGGCAAAGTC GACGGATCCG GTACCGATTA CAAGGACGAC
            661 GATGACAAGT AGTACTGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT
            721 GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT
            781 GTCTGANTAN GTGTCCTTCT ATAATATTAT GGGGTGGAGG GNGGTGGTAT GGAGCAAGGG
            841 GCAAGTTGGG AAGANAACCT GTAGGGCCTG CNGGGTCTAT NGGNAACCNA GCTGGANTGC
            901 AGTGGCNCAA TCTTGGCNCA NNGCNATCTC CGCCTCCNNG GNTCNAGCNA TTCTCCNGNC
            961 NNCANCCTCC CNNNNNNNTN GGNNNTNNNN NNATGCATGA NNANNCNCAN NNNANNTTTN
          1021 NNTTTTTTNG NNNNANNNNG GNNTNNNNCN NNNNNNNNCN NNNNNNNNN
          //
          Primer: SV40pro_F
          Region: SV40 pro_ori, lac op, lac pro, CAP bs, ori
          Sequence file: RDB17330_A9Ehb.seq check
          >D06321A7_A9Eh_2_SV40pro_F_D06_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGA NNNGTGAGGA NGNTTTTTTG GAGGNCTAGG CTTTTGCAAA
             61 AAGCTCCTCG AGGAACTGAA AAACCAGAAA GTTAATTCCC TATAGTGAGT CGTATTAAAT
            121 TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
            181 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
            241 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
            301 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT
            361 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
            421 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
            481 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
            541 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
            601 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
            661 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
            721 TTTCTCATAG CTCACGCTGT ANGTATCTCA GTTCGGTGTA NGTCGTTCGC TCCNAGCTGG
            781 GCTGTGTGCA CGAACCCNNC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
            841 TTGAGTCCNA CCCGGTAAGA NACGACTTAT CGCCNCNGGC AGCAGCCACT GGNAACAGGA
            901 NTAGCANANC GNNGTATGTA NGNGGNGCTA CAGANTTCTN GNAGNGNNGG NCNNNNNANN
          961 NNACNCTANN NGANNAGNAN TNNNNANNNG CNCNNNGNNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kayama, K., GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO Rep. 18 (1): 123-137 (2017). PMID 27856536.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl