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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFLAG-CMV-5a-RPL11 (#RDB17330)

Expression vector of human RPL11 tagged with FLAG at C-terminus.

Clone info. Expression vector of human ribosomal protein L11 (RPL11) tagged with FLAG at C-terminus, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pFLAG-CMV-5a (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name human RPL11 cDNA
Depositor|Developer Fujita, Masatoshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kayama K. et al. EMBO Rep. 18, 123-137, 2017).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kayama K. et al. EMBO Rep. 18, 123-137, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17330 pFLAG-CMV-5a-RPL11 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFLAG-CMV-5a-RPL11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17330).

Reference section:

Kayama, K., Watanabe, S., Takafuji, T., Tsuji, T., Hironaka, K., Matsumoto, M., Nakayama, K.I., Enari, M., Kohno, T., Shiraishi, K., Kiyono, T., Yoshida, K., Sugimoto, N., Fujita, M., GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO Rep. 18 (1): 123-137 (2017). PMID 27856536. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17330_A9Ehp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert, FLAG, hGH pA
Sequence file: RDB17330_A9Eha.seq check
>D06321A7_A9Eh_2_CMV_Forward_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNGN NNCNNATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTCACCATGG CGGATCAAGG TGAAAAGGAG
  121 AACCCCATGC GGGAACTTCG CATCCGCAAA CTCTGTCTCA ACATCTGTGT TGGGGAGAGT
  181 GGAGACAGAC TGACGCGAGC AGCCAAGGTG TTGGAGCAGC TCACAGGGCA GACCCCTGTG
  241 TTTTCCAAAG CTAGATACAC TGTCAGATCC TTTGGCATCC GGAGAAATGA AAAGATTGCT
  301 GTCCACTGCA CAGTTCGAGG GGCCAAGGCA GAAGAAATCT TGGAGAAGGG TCTAAAGGTG
  361 CGGGAGTATG AGTTAAGAAA AAACAACTTC TCAGATACTG GAAACTTTGG TTTTGGGATC
  421 CAGGAACACA TCGATCTGGG TATCAAATAT GACCCAAGCA TTGGTATCTA CGGCCTGGAC
  481 TTCTATGTGG TGCTGGGTAG GCCAGGTTTC AGCATCGCAG ACAAGAAGCG CAGGACAGGC
  541 TGCATTGGGG CCAAACACAG AATCAGCAAA GAGGAGGCCA TGCGCTGGTT CCAGCAGAAG
  601 TATGATGGGA TCATCCTTCC TGGCAAAGTC GACGGATCCG GTACCGATTA CAAGGACGAC
  661 GATGACAAGT AGTACTGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT
  721 GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT
  781 GTCTGANTAN GTGTCCTTCT ATAATATTAT GGGGTGGAGG GNGGTGGTAT GGAGCAAGGG
  841 GCAAGTTGGG AAGANAACCT GTAGGGCCTG CNGGGTCTAT NGGNAACCNA GCTGGANTGC
  901 AGTGGCNCAA TCTTGGCNCA NNGCNATCTC CGCCTCCNNG GNTCNAGCNA TTCTCCNGNC
  961 NNCANCCTCC CNNNNNNNTN GGNNNTNNNN NNATGCATGA NNANNCNCAN NNNANNTTTN
1021 NNTTTTTTNG NNNNANNNNG GNNTNNNNCN NNNNNNNNCN NNNNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori, lac op, lac pro, CAP bs, ori
Sequence file: RDB17330_A9Ehb.seq check
>D06321A7_A9Eh_2_SV40pro_F_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGA NNNGTGAGGA NGNTTTTTTG GAGGNCTAGG CTTTTGCAAA
   61 AAGCTCCTCG AGGAACTGAA AAACCAGAAA GTTAATTCCC TATAGTGAGT CGTATTAAAT
  121 TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
  181 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
  241 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
  301 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT
  361 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
  421 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
  481 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
  541 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
  601 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
  661 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
  721 TTTCTCATAG CTCACGCTGT ANGTATCTCA GTTCGGTGTA NGTCGTTCGC TCCNAGCTGG
  781 GCTGTGTGCA CGAACCCNNC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
  841 TTGAGTCCNA CCCGGTAAGA NACGACTTAT CGCCNCNGGC AGCAGCCACT GGNAACAGGA
  901 NTAGCANANC GNNGTATGTA NGNGGNGCTA CAGANTTCTN GNAGNGNNGG NCNNNNNANN
961 NNACNCTANN NGANNAGNAN TNNNNANNNG CNCNNNGNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kayama, K., GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO Rep. 18 (1): 123-137 (2017). PMID 27856536. [link to RRC of NBRP]