Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFLAG-CMV-5a-RPL5

Expression vector of human RPL5 tagged with FLAG at C-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kayama K. et al. EMBO Rep. 18, 123-137, 2017).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kayama K. et al. EMBO Rep. 18, 123-137, 2017)。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17329_A9Ehp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5' Sequence file: RDB17329_A9Eha.seq
>D06321A6_A9Eh_1_CMV_Forward_E06_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNG NNNCNNTGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTCACCATGG GGTTTGTTAA AGTTGTTAAG   121 AATAAGGCCT ACTTTAAGAG ATACCAAGTG AAATTTAGAA GACGACGAGA GGGTAAAACT   181 GATTATTATG CTCGGAAACG CTTGGTGATA CAAGATAAAA ATAAATACAA CACACCCAAA   241 TACAGGATGA TAGTTCGTGT GACAAACAGA GATATCATTT GTCAGATTGC TTATGCCCGT   301 ATAGAGGGGG ATATGATAGT CTGCGCAGCG TATGCACACG AACTGCCAAA ATATGGTGTG   361 AAGGTTGGCC TGACAAATTA TGCTGCAGCA TATTGTACTG GCCTGCTGCT GGCCCGCAGG   421 CTTCTCAATA GGTTTGGCAT GGACAAGATC TATGAAGGCC AAGTGGAGGT GACTGGTGAT   481 GAATACAATG TGGAAAGCAT TGATGGTCAG CCAGGTGCCT TCACCTGCTA TTTGGATGCA   541 GGCCTTGCCA GAACTACCAC TGGCAATAAA GTTTTTGGTG CCCTGAAGGG AGCTGTGGAT   601 GGAGGCTTGT CTATCCCTCA CAGTACCAAA CGATTCCCTG GTTATGATTC TGAAAGCAAG   661 GAATTTAATG CAGAAGTACA TCGGAAGCAC ATCATGGGCC AGAATGTTGC AGATTACATG   721 CGCTACTTAA TGGAAGAAGA NGAAGATGCT TACAAGAAAC AGTTCTCTCA ATACATAAAG   781 AACAGCGTAA CTCCNGACAT GATGGANGAG ANGTATAAGA AAGCTCATGC TGCTATACGA   841 GAGAATCCAG TCTATGAAAA GAAGCCCAAG AAAGAAGTTA AAAAGAAGAN GTGGAANCGT   901 CCCNAAATGT CCNNNGCNCN NAANAANNNT CNGGTANCTC AAAANAANNN NNGCTTNCNC 961 ANANCTCNNN NCGGGCNGNN NANNNCGNCN NNNNNNCNNN NANNNANTAN NNNNNNNNN //
Primer: hGHpolyA-R (Pr0350) Region: hGH pA, FLAG, insert 3' Sequence file: RDB17329_A9Ehb.seq
>D06321A6_A9Eh_1_hGHpolyA-R_F06_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCCN NNAATATTAT AGAAGGACNC CTAGTCAGAC AAAATGATGC    61 AACTTAATTT TATTAGGACA AGGCTGGTGG GCACTGGAGT GGCAACTTCC AGGGCCAGGA   121 GAGGCACTGG GGAGGGGTCA CAGGGATGCC ACCCAGTACT ACTTGTCATC GTCGTCCTTG   181 TAATCGGTAC CGGATCCGTC GACGCTCTCA GCAGCCCGCT CCTGAGCTCT GAGGAAGCTT   241 GCCTTCTTTT GAGCTACCCG ATCCTTCTTC TGAGCAAGGG ACATTTTGGG ACGGTTCCAC   301 CTCTTCTTTT TAACTTCTTT CTTGGGCTTC TTTTCATAGA CTGGATTCTC TCGTATAGCA   361 GCATGAGCTT TCTTATACAT CTCCTCCATC ATGTCTGGAG TTACGCTGTT CTTTATGTAT   421 TGAGAGAACT GTTTCTTGTA AGCATCTTCA TCTTCTTCCA TTAAGTAGCG CATGTAATCT   481 GCAACATTCT GGCCCATGAT GTGCTTCCGA TGTACTTCTG CATTAAATTC CTTGCTTTCA   541 GAATCATAAC CAGGGAATCG TTTGGTACTG TGAGGGATAG ACAAGCCTCC ATCCACAGCT   601 CCCTTCAGGG CACCAAAAAC TTTATTGCCA GTGGTAGTTC TGGCAAGGCC TGCATCCAAA   661 TAGCAGGTGA AGGCACCTGG CTGACCATCA ATGCTTTCCA CATTGTATTC ATCACCAGTC   721 ACCTCCACTT GGCCTTCATA GATCTTGTCC ATGCCAAACC TATTGAGAAG CCTGCGGGCC   781 AGCAGCANGC CAGTACAATA TGCTGCAGCA TAATTTGTCA NGNCNACCTT CACACCATAT   841 TTTGGCAGTT CGTGTGCATA CGCTGCGCAN ANTATCATAT CCCCNTCTAN ACNGGCATNA   901 GCNATCTGAC AAATGANATC TCTGTTTGNC NCACNAACTA TCATCCNGTA TTTGGGNGNG   961 NNGNATTTAT TTTTANCTNG NATCNNNNAN CNNTTNNNAN CATNNNNNNC ANNTTNNNCN 1021 NNNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori, lac op, lac pro, CAP bs, ori Sequence file: RDB17329_A9Ehc.seq
>D06321A6_A9Eh_1_SV40pro_F_G06_21_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGA NNNGTGAGGA NGNTTTTTTG GAGGCCTAGG CTTTTGCAAA    61 AAGCTCCTCG AGGAACTGAA AAACCAGAAA GTTAATTCCC TATAGTGAGT CGTATTAAAT   121 TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA   181 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA   241 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC   301 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT   361 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT   421 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG   481 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA   541 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC   601 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG   661 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC   721 TTTCTCATAG CTCACGCTGT ANGTATCTCA GTTCGGTGTA NGTCGTTCGC TCCAAGCTGG   781 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC   841 TTGANTCCNA CCCGGTAAGA NACGACTTAT CGCCNCTGGC AGCAGCCNCN GGTAACANGA   901 NTAGCANANC GAGGNATGTA NGNGGNGCTA CAGANTNCTT GAAGNNGNGG NCTNNCNANN   961 NNTACNCTAN NANNAACAGT ATTTGGTATN NGNNNNCNNC TGNNNCNNNN TNANNNNNNN 1021 NAAAANNNNT NNGNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl