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pFLAG-CMV-5a-RPL5

Expression vector of human RPL5 tagged with FLAG at C-terminus.

Catalog number RDB17329
Resource name pFLAG-CMV-5a-RPL5
Clone info. Expression vector of human ribosomal protein L5 (RPL5) tagged with FLAG at C-terminus, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pFLAG-CMV-5a (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name human RPL5 cDNA
Depositor|Developer Fujita, Masatoshi |
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External Database
human RPL5

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kayama K. et al. EMBO Rep. 18, 123-137, 2017).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kayama K. et al. EMBO Rep. 18, 123-137, 2017)。
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          RDB17329 pFLAG-CMV-5a-RPL5 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kayama, K., GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO Rep. 18 (1): 123-137 (2017). PMID 27856536.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17329_A9Ehp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, insert 5'
          Sequence file: RDB17329_A9Eha.seq check
          >D06321A6_A9Eh_1_CMV_Forward_E06_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTNG NNNCNNTGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTCACCATGG GGTTTGTTAA AGTTGTTAAG
            121 AATAAGGCCT ACTTTAAGAG ATACCAAGTG AAATTTAGAA GACGACGAGA GGGTAAAACT
            181 GATTATTATG CTCGGAAACG CTTGGTGATA CAAGATAAAA ATAAATACAA CACACCCAAA
            241 TACAGGATGA TAGTTCGTGT GACAAACAGA GATATCATTT GTCAGATTGC TTATGCCCGT
            301 ATAGAGGGGG ATATGATAGT CTGCGCAGCG TATGCACACG AACTGCCAAA ATATGGTGTG
            361 AAGGTTGGCC TGACAAATTA TGCTGCAGCA TATTGTACTG GCCTGCTGCT GGCCCGCAGG
            421 CTTCTCAATA GGTTTGGCAT GGACAAGATC TATGAAGGCC AAGTGGAGGT GACTGGTGAT
            481 GAATACAATG TGGAAAGCAT TGATGGTCAG CCAGGTGCCT TCACCTGCTA TTTGGATGCA
            541 GGCCTTGCCA GAACTACCAC TGGCAATAAA GTTTTTGGTG CCCTGAAGGG AGCTGTGGAT
            601 GGAGGCTTGT CTATCCCTCA CAGTACCAAA CGATTCCCTG GTTATGATTC TGAAAGCAAG
            661 GAATTTAATG CAGAAGTACA TCGGAAGCAC ATCATGGGCC AGAATGTTGC AGATTACATG
            721 CGCTACTTAA TGGAAGAAGA NGAAGATGCT TACAAGAAAC AGTTCTCTCA ATACATAAAG
            781 AACAGCGTAA CTCCNGACAT GATGGANGAG ANGTATAAGA AAGCTCATGC TGCTATACGA
            841 GAGAATCCAG TCTATGAAAA GAAGCCCAAG AAAGAAGTTA AAAAGAAGAN GTGGAANCGT
            901 CCCNAAATGT CCNNNGCNCN NAANAANNNT CNGGTANCTC AAAANAANNN NNGCTTNCNC
          961 ANANCTCNNN NCGGGCNGNN NANNNCGNCN NNNNNNCNNN NANNNANTAN NNNNNNNNN
          //
          Primer: hGHpolyA-R
          Region: hGH pA, FLAG, insert 3'
          Sequence file: RDB17329_A9Ehb.seq check
          >D06321A6_A9Eh_1_hGHpolyA-R_F06_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNCCN NNAATATTAT AGAAGGACNC CTAGTCAGAC AAAATGATGC
             61 AACTTAATTT TATTAGGACA AGGCTGGTGG GCACTGGAGT GGCAACTTCC AGGGCCAGGA
            121 GAGGCACTGG GGAGGGGTCA CAGGGATGCC ACCCAGTACT ACTTGTCATC GTCGTCCTTG
            181 TAATCGGTAC CGGATCCGTC GACGCTCTCA GCAGCCCGCT CCTGAGCTCT GAGGAAGCTT
            241 GCCTTCTTTT GAGCTACCCG ATCCTTCTTC TGAGCAAGGG ACATTTTGGG ACGGTTCCAC
            301 CTCTTCTTTT TAACTTCTTT CTTGGGCTTC TTTTCATAGA CTGGATTCTC TCGTATAGCA
            361 GCATGAGCTT TCTTATACAT CTCCTCCATC ATGTCTGGAG TTACGCTGTT CTTTATGTAT
            421 TGAGAGAACT GTTTCTTGTA AGCATCTTCA TCTTCTTCCA TTAAGTAGCG CATGTAATCT
            481 GCAACATTCT GGCCCATGAT GTGCTTCCGA TGTACTTCTG CATTAAATTC CTTGCTTTCA
            541 GAATCATAAC CAGGGAATCG TTTGGTACTG TGAGGGATAG ACAAGCCTCC ATCCACAGCT
            601 CCCTTCAGGG CACCAAAAAC TTTATTGCCA GTGGTAGTTC TGGCAAGGCC TGCATCCAAA
            661 TAGCAGGTGA AGGCACCTGG CTGACCATCA ATGCTTTCCA CATTGTATTC ATCACCAGTC
            721 ACCTCCACTT GGCCTTCATA GATCTTGTCC ATGCCAAACC TATTGAGAAG CCTGCGGGCC
            781 AGCAGCANGC CAGTACAATA TGCTGCAGCA TAATTTGTCA NGNCNACCTT CACACCATAT
            841 TTTGGCAGTT CGTGTGCATA CGCTGCGCAN ANTATCATAT CCCCNTCTAN ACNGGCATNA
            901 GCNATCTGAC AAATGANATC TCTGTTTGNC NCACNAACTA TCATCCNGTA TTTGGGNGNG
            961 NNGNATTTAT TTTTANCTNG NATCNNNNAN CNNTTNNNAN CATNNNNNNC ANNTTNNNCN
          1021 NNNNNNNNN
          //
          Primer: SV40pro_F
          Region: SV40 pro_ori, lac op, lac pro, CAP bs, ori
          Sequence file: RDB17329_A9Ehc.seq check
          >D06321A6_A9Eh_1_SV40pro_F_G06_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGA NNNGTGAGGA NGNTTTTTTG GAGGCCTAGG CTTTTGCAAA
             61 AAGCTCCTCG AGGAACTGAA AAACCAGAAA GTTAATTCCC TATAGTGAGT CGTATTAAAT
            121 TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
            181 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
            241 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
            301 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT
            361 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
            421 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
            481 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
            541 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
            601 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
            661 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
            721 TTTCTCATAG CTCACGCTGT ANGTATCTCA GTTCGGTGTA NGTCGTTCGC TCCAAGCTGG
            781 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
            841 TTGANTCCNA CCCGGTAAGA NACGACTTAT CGCCNCTGGC AGCAGCCNCN GGTAACANGA
            901 NTAGCANANC GAGGNATGTA NGNGGNGCTA CAGANTNCTT GAAGNNGNGG NCTNNCNANN
            961 NNTACNCTAN NANNAACAGT ATTTGGTATN NGNNNNCNNC TGNNNCNNNN TNANNNNNNN
          1021 NAAAANNNNT NNGNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kayama, K., GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO Rep. 18 (1): 123-137 (2017). PMID 27856536.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl