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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFLAG-CMV-5a-RPL5 (#RDB17329)

Expression vector of human RPL5 tagged with FLAG at C-terminus.

Clone info. Expression vector of human ribosomal protein L5 (RPL5) tagged with FLAG at C-terminus, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pFLAG-CMV-5a (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name human RPL5 cDNA
Depositor|Developer Fujita, Masatoshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kayama K. et al. EMBO Rep. 18, 123-137, 2017).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kayama K. et al. EMBO Rep. 18, 123-137, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17329 pFLAG-CMV-5a-RPL5 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFLAG-CMV-5a-RPL5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17329).

Reference section:

Kayama, K., Watanabe, S., Takafuji, T., Tsuji, T., Hironaka, K., Matsumoto, M., Nakayama, K.I., Enari, M., Kohno, T., Shiraishi, K., Kiyono, T., Yoshida, K., Sugimoto, N., Fujita, M., GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO Rep. 18 (1): 123-137 (2017). PMID 27856536. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17329_A9Ehp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5'
Sequence file: RDB17329_A9Eha.seq check
>D06321A6_A9Eh_1_CMV_Forward_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNG NNNCNNTGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTCACCATGG GGTTTGTTAA AGTTGTTAAG
  121 AATAAGGCCT ACTTTAAGAG ATACCAAGTG AAATTTAGAA GACGACGAGA GGGTAAAACT
  181 GATTATTATG CTCGGAAACG CTTGGTGATA CAAGATAAAA ATAAATACAA CACACCCAAA
  241 TACAGGATGA TAGTTCGTGT GACAAACAGA GATATCATTT GTCAGATTGC TTATGCCCGT
  301 ATAGAGGGGG ATATGATAGT CTGCGCAGCG TATGCACACG AACTGCCAAA ATATGGTGTG
  361 AAGGTTGGCC TGACAAATTA TGCTGCAGCA TATTGTACTG GCCTGCTGCT GGCCCGCAGG
  421 CTTCTCAATA GGTTTGGCAT GGACAAGATC TATGAAGGCC AAGTGGAGGT GACTGGTGAT
  481 GAATACAATG TGGAAAGCAT TGATGGTCAG CCAGGTGCCT TCACCTGCTA TTTGGATGCA
  541 GGCCTTGCCA GAACTACCAC TGGCAATAAA GTTTTTGGTG CCCTGAAGGG AGCTGTGGAT
  601 GGAGGCTTGT CTATCCCTCA CAGTACCAAA CGATTCCCTG GTTATGATTC TGAAAGCAAG
  661 GAATTTAATG CAGAAGTACA TCGGAAGCAC ATCATGGGCC AGAATGTTGC AGATTACATG
  721 CGCTACTTAA TGGAAGAAGA NGAAGATGCT TACAAGAAAC AGTTCTCTCA ATACATAAAG
  781 AACAGCGTAA CTCCNGACAT GATGGANGAG ANGTATAAGA AAGCTCATGC TGCTATACGA
  841 GAGAATCCAG TCTATGAAAA GAAGCCCAAG AAAGAAGTTA AAAAGAAGAN GTGGAANCGT
  901 CCCNAAATGT CCNNNGCNCN NAANAANNNT CNGGTANCTC AAAANAANNN NNGCTTNCNC
961 ANANCTCNNN NCGGGCNGNN NANNNCGNCN NNNNNNCNNN NANNNANTAN NNNNNNNNN
//
Primer: hGHpolyA-R (Pr0350)
Region: hGH pA, FLAG, insert 3'
Sequence file: RDB17329_A9Ehb.seq check
>D06321A6_A9Eh_1_hGHpolyA-R_F06_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCCN NNAATATTAT AGAAGGACNC CTAGTCAGAC AAAATGATGC
   61 AACTTAATTT TATTAGGACA AGGCTGGTGG GCACTGGAGT GGCAACTTCC AGGGCCAGGA
  121 GAGGCACTGG GGAGGGGTCA CAGGGATGCC ACCCAGTACT ACTTGTCATC GTCGTCCTTG
  181 TAATCGGTAC CGGATCCGTC GACGCTCTCA GCAGCCCGCT CCTGAGCTCT GAGGAAGCTT
  241 GCCTTCTTTT GAGCTACCCG ATCCTTCTTC TGAGCAAGGG ACATTTTGGG ACGGTTCCAC
  301 CTCTTCTTTT TAACTTCTTT CTTGGGCTTC TTTTCATAGA CTGGATTCTC TCGTATAGCA
  361 GCATGAGCTT TCTTATACAT CTCCTCCATC ATGTCTGGAG TTACGCTGTT CTTTATGTAT
  421 TGAGAGAACT GTTTCTTGTA AGCATCTTCA TCTTCTTCCA TTAAGTAGCG CATGTAATCT
  481 GCAACATTCT GGCCCATGAT GTGCTTCCGA TGTACTTCTG CATTAAATTC CTTGCTTTCA
  541 GAATCATAAC CAGGGAATCG TTTGGTACTG TGAGGGATAG ACAAGCCTCC ATCCACAGCT
  601 CCCTTCAGGG CACCAAAAAC TTTATTGCCA GTGGTAGTTC TGGCAAGGCC TGCATCCAAA
  661 TAGCAGGTGA AGGCACCTGG CTGACCATCA ATGCTTTCCA CATTGTATTC ATCACCAGTC
  721 ACCTCCACTT GGCCTTCATA GATCTTGTCC ATGCCAAACC TATTGAGAAG CCTGCGGGCC
  781 AGCAGCANGC CAGTACAATA TGCTGCAGCA TAATTTGTCA NGNCNACCTT CACACCATAT
  841 TTTGGCAGTT CGTGTGCATA CGCTGCGCAN ANTATCATAT CCCCNTCTAN ACNGGCATNA
  901 GCNATCTGAC AAATGANATC TCTGTTTGNC NCACNAACTA TCATCCNGTA TTTGGGNGNG
  961 NNGNATTTAT TTTTANCTNG NATCNNNNAN CNNTTNNNAN CATNNNNNNC ANNTTNNNCN
1021 NNNNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori, lac op, lac pro, CAP bs, ori
Sequence file: RDB17329_A9Ehc.seq check
>D06321A6_A9Eh_1_SV40pro_F_G06_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGA NNNGTGAGGA NGNTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCTCG AGGAACTGAA AAACCAGAAA GTTAATTCCC TATAGTGAGT CGTATTAAAT
  121 TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
  181 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
  241 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
  301 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT
  361 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
  421 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
  481 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
  541 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
  601 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
  661 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
  721 TTTCTCATAG CTCACGCTGT ANGTATCTCA GTTCGGTGTA NGTCGTTCGC TCCAAGCTGG
  781 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
  841 TTGANTCCNA CCCGGTAAGA NACGACTTAT CGCCNCTGGC AGCAGCCNCN GGTAACANGA
  901 NTAGCANANC GAGGNATGTA NGNGGNGCTA CAGANTNCTT GAAGNNGNGG NCTNNCNANN
  961 NNTACNCTAN NANNAACAGT ATTTGGTATN NGNNNNCNNC TGNNNCNNNN TNANNNNNNN
1021 NAAAANNNNT NNGNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kayama, K., GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis. EMBO Rep. 18 (1): 123-137 (2017). PMID 27856536. [link to RRC of NBRP]