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pET47-eIF4A1-19-406 (#RDB17294)

Bacterial expression vecter of mouse eIF4A1 fragment from Pro19 to C-terminal Ile406.

Clone info. Bacterial expression vecter of mouse eukaryotic translation initiation factor 4A1 (eIF4A1) fragment from Pro19 to C-terminal Ile406 tagged with 6xHis at N-terminus.
Comment Expression was confirmed by the depositor with CBB staining. This clone contains a cDNA fragment corresponding to the 177nt to 1343nt of NM_144958.4. Known differences (at least) : G to C substitution at 179nt (P, synonym) ; C to T substitution at 1325nt (N, synonym) ; T to C substitution at 1340nt (I, synonym).
Vector backbone pET47 (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Kan^r.
Gene/insert name Mouse Eif4a1 cDNA
Depositor|Developer Iwasaki, Shintaro |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019).
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17294 pET47-eIF4A1-19-406 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET47-eIF4A1-19-406 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17294).

Reference section:

Iwasaki, S., Iwasaki, W., Takahashi, M., Sakamoto, A., Watanabe, C., Shichino, Y., Floor, S.N., Fujiwara, K., Mito, M., Dodo, K., Sodeoka, M., Imataka, H., Honma, T., Fukuzawa, K., Ito, T., Ingolia, N.T., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17294_A9Dbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR_BamHI_R2 (Pr0141)
Region: T7 pro,lacO,RBS,6xHis,HRV 3C site,insert 5'
Sequence file: RDB17294_A9Dba.seq check
>D06253A1_A9Db_4_pBR_BamHI_R2_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NNCGNNNGGC NNANNNGNNC GAGATCGATC
   61 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC
  121 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCAC ATCACCACCA
  181 CCATCACTCC GCGGCTCTTG AAGTCCTCTT TCAGGGACCC GGGTACCAGG ATCCCGAAGG
  241 CGTCATCGAG AGTAACTGGA ACGAGATTGT GGATAGCTTT GATGACATGA ATCTCTCAGA
  301 GTCCCTCCTC CGTGGTATTT ATGCCTATGG TTTTGAGAAG CCCTCTGCCA TCCAGCAGCG
  361 AGCTATTCTT CCTTGTATCA AGGGTTATGA TGTGATTGCT CAAGCCCAGT CTGGGACTGG
  421 GAAAACAGCT ACATTTGCCA TATCAATTCT GCAGCAGATT GAATTAGATC TAAAGGCCAC
  481 TCAGGCTTTG GTTCTGGCAC CCACACGTGA ATTGGCTCAG CAGATACAAA AGGTGGTTAT
  541 GGCATTAGGA GACTACATGG GTGCCTCTTG TCATGCCTGC ATTGGGGGCA CCAATGTGCG
  601 TGCTGAGGTG CAGAAGCTGC AGATGGAAGC TCCCCATATC ATCGTGGGTA CCCCTGGCCG
  661 GGTGTTTGAC ATGCTTAACC GGAGNTACCT GTCCCCCAAA TACATCAAGA TGTTCGTACT
  721 GGATGAAGCA NANGAAATGT TAAGCCNANG GTTCNAGGAT CANATCTATG ANATATTCCA
  781 NAAGCTCAAC NGCAACACAC AGGTAGTTTT GTTGTCTGCT ACNATGCCTT CTGATGTNCT
  841 TGANGTGANN NANAANTTTA TGANAGANCC TANTNNGNNT CNTGTNAAGA ANGGAANANN
  901 TGANCCNNGN ANGGNANNCN CCNNTTCTNC NTNNATGTGN AANGAGANGA NTGGNAANCT
961 TGNNNNNNTN NTGNGANNNG NATGANNNNC TNANNATNNN CCAGNNNNNN NNNN
//
Primer: f1ori_R3 (Pr0142)
Region: T7 ter,insert 3'
Sequence file: RDB17294_A9Dbb.seq check
>D06253A1_A9Db_4_f1ori_R3_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCCTTNNNG CNNNANCCCT CAAGANCCGT TTAGAGGCCC
   61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
  121 CTTTGTTTAG CAGCCTAGGT TAATTAAGCC TCGAGAGCAG CAGAAGTAGA GCTGTCCATG
  181 TGCTGGCGTT CGAATTTAGC AGCAGCGGTT TCTTTACTAC CGCGTGGCAC CAGAGCGAGC
  241 TCTGCGGCCG CAAGCTTGTC GACTCAGATG AGGTCAGCAA CATTGAGGGG CATCTCTTCA
  301 ATGGAGGTGT TGTAGAAAGT CTCAATGTCT CGAAGAGTCC TCTTGTCTTC TTCGGTCACC
  361 ATGTTAATAG CCACACCCTT ACGACCAAAC CGACCACCTC GACCGATTCT GTGGATGTAG
  421 TTTTCCCTGT TGGTGGGAAG GTCATAGTTG ATGACTAAGG AGACCTGCTG CACATCAATG
  481 CCTCTGGCCA ACAGGTCAGT GGTAATTAAT ACTCTGCTAG AGCCAGACCG GAACTCCCTC
  541 ATGATCACAT CTCGTTCCTT TTGGTCCATA TCTCCGTGCA TGGCAGAAAC AGTGAAATCT
  601 CGGGCATGCA TCTTCTCGGT GAGCCAGTCC ACCTTCCTTC TGGTGTTGAT AAAGATGACT
  661 GCCTGGGTGA TGGTCAGCGT CTCATACAAG TCACACAATG TGTCAAGCTT CCACTCCTCT
  721 CGTTCCACAT TGATGTAGAA TTGGCGGANA CCCTCCAGGG TCAATTCTTC CTTCTTGACA
  781 AGAATCCGAA TAGGGTCTCT CATAAATTTC TTGGTCACCT CAAGGACATC AGAANGCATT
  841 GTANCAGANA ACANAACTAC CTGTGTGTTG CTGTTGANCT TCTGGAATAT GTCATANATC
  901 TGATNCTTNA ANCCNCGGCT NAACNTTTCA TCTGCTNCAT CNNNNACGAA NNTCTNNNTG
  961 TATTTNGGGG GANAGGTATN NNCNNNNNNN NCANGNNAAA NNCCNNNNCN NNGGNNNNNC
1021 NNNNNNNNNN
//
Primer: lacIpro_F (Pr0409)
Region: lacI pro,lacI
Sequence file: RDB17294_A9Dbc.seq check
>D06253A1_A9Db_4_laclpro_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNATTC NGGGTGGTGA ATGTGAAACC
   61 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT
  121 GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC
  181 GGAGCTGAAT TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT
  241 GATTGGCGTT GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT
  301 TAAATCTCGC GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG
  361 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT
  421 CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT
  481 TCCGGCGTTA TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA
  541 TGAAGACGGT ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC
  601 GCTGTTAGCG GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA
  661 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT
  721 GTCCGGTTTT CAACAAACCA TGCAAATGCT GAANNGAGGG CATCGTTCCC ACTGCGATGC
  781 TGGTTGCCAN CGATCAGATG GCGCTGGGCG CANTGCGCGC CATTACCNAG TCNNGGCTGC
  841 NCGTTGGTGC GGNNATCTCN NNNNNTGGGA TANNANNNAT ACCNAANACA GCTCNNGTTA
  901 TATCCCGCCG TTAACCACCN TCAAANNNGN NTTTCNNCNG CNNGGGGCAA ACNNNCNNGN
961 ANCNNNTNGC TGNNNCTCTC TCNNGNCNNN NNNNNGANNG NNAANNANCN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437. [link to RRC of NBRP]