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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab32 (#RDB17277)

Expression vector of mouse Rab32 fused with EGFP.

Clone info. Expression vector of mouse Rab32 fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab32 cDNA
Reference of insert sequence AB232627 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17277 pEGFP-C1-Rab32 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab32 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17277).

Reference section:

Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17277_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3',Insert 5'
Sequence file: RDB17277_A9Ela.seq check
>D06280G5_A9El_1_EGFP-C2_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN TCNNNTGGTC CTGCTGGANT TCGTGACCGC CGCCGGGNTC
   61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGGCGGG CGAGGGACTA
  121 GGGCAACAGG GCGCGTCGGC CACCGCGGCG CCGGAGACCC GAGAGCACCT CTTTAAGGTG
  181 CTGGTCATCG GCGAGCTCGG CGTGGGTAAG ACGAGCATCA TCAAGCGCTA CGTGCACCAG
  241 CTCTTCTCCC AGCACTACCG CGCCACCATT GGCGTGGACT TCGCCCTCAA AGTTCTCAAC
  301 TGGGACAGCA GGACGCTCGT GCGCCTGCAG CTGTGGGATA TCGCGGGACA GGAACGGTTT
  361 GGCAACATGA CTCGAGTATA CTATAAGGAA GCTCTTGGAG CATTTGTAGT CTTTGATATA
  421 TCGAGAAGTT CTACATTTGA TGCAGTCCTA AAATGGAAAA ACGATCTGGA TAGTAAAGTT
  481 CATCTTCCCA ACGGCAGCCC CATTCCTGCT GTCCTCCTAG CTAACAAATG TGACCAGAAG
  541 AAGGACAACA GCCAGAGTCC TTCCCAGATG GACCAGTTTT GTAAAGACCA TGGCTTCACT
  601 GGATGGTTTG AAACCTCTGC CAAGGATAAT ATAAACATCG ACGAGGCCAC CCGCTTCCTA
  661 GTGGAAAACA TGCTTGCAAA CCAGCAAAGT TTTCCTAGTG AAGAAATCGA CCTGGACAGA
  721 ATTAAACTGG TAGAGGAGCC CCCCACAACA AAGCCCAGGT CCCAGTGCTG CTGANGTGTC
  781 TCTGTGAATC ACTAGTGAAT TCTGCAGTCG ANGGTACCGC GGGCCCGGGA TCCACCGGAT
  841 CTAGATAACT GATCATAATC AGCCNTACNA CATTTGNAGA NGNTTNACTT GCTTTAAAAA
  901 ANCTCCCNCA NCNCCCCCTG AANCNGAANN ATAAANNGAN TGNNATTGNN GNNGNNAACT
  961 TGNTTANNGN NGCTNANNAT GGNNNNNAAN NAANCNNNAN CNTCNCAANT TTCNNNAANN
1021 ANNNNNTTTT TTNNNNNNNN NN
//
Primer: SV40polyA-R (Pr0109)
Region: insert 3'
Sequence file: RDB17277_A9Elb.seq check
>D06280G5_A9El_1_SV40polyA-R2_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNGNNNNNAA NNNGTTAANN ANNACNATTG CATTCNTTTT ATGTTTCNGG
   61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA
  181 GAATTCACTA GTGATTCACA GAGACACATC AGCAGCACTG GGACCTGGGC TTTGTTGTGG
  241 GGGGCTCCTC TACCAGTTTA ATTCTGTCCA GGTCGATTTC TTCACTAGGA AAACTTTGCT
  301 GGTTTGCAAG CATGTTTTCC ACTAGGAAGC GGGTGGCCTC GTCGATGTTT ATATTATCCT
  361 TGGCAGAGGT TTCAAACCAT CCAGTGAAGC CATGGTCTTT ACAAAACTGG TCCATCTGGG
  421 AAGGACTCTG GCTGTTGTCC TTCTTCTGGT CACATTTGTT AGCTAGGAGG ACAGCAGGAA
  481 TGGGGCTGCC GTTGGGAAGA TGAACTTTAC TATCCAGATC GTTTTTCCAT TTTAGGACTG
  541 CATCAAATGT AGAACTTCTC GATATATCAA AGACTACAAA TGCTCCAAGA GCTTCCTTAT
  601 AGTATACTCG AGTCATGTTG CCAAACCGTT CCTGTCCCGC GATATCCCAC AGCTGCAGGC
  661 GCACGAGCGT CCTGCTGTCC CAGTTGAGAA CTTTGAGGGC GAAGTCCACG CCAATGGTGG
  721 CGCGGTAGTG CTGGGAGAAG AGCTGGTGCA CGTAGCGCTT GATGATGCTC GTCTTACCCA
  781 CGCCGANCTC GCCGATGANC AGCACCTTAA AGANGTGCTC TCGGGTCTCC GGCGCCGCGG
  841 NGGCCGACGC GCCCTGTTGC CCTAGNCCCN CGCCCGCCAN GGATCNNANN NCNGANTTGT
  901 ACNGCTCGNC CNTGCCNANA NTGATNCCGG CGGNNNNCAC GAACTCCAGC ANGANCNTGN
961 NNTCGCGCTT CNCNNTNNGG GNNTTTGNTC NNNNNGNNNN NGNNGNNCNN NNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: EGFP 5'
Sequence file: RDB17277_A9Elc.seq check
>D06280G5_A9El_1_CMV_Forward_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNGC NNTGGGCGGT AGGCGTNNTA CGGTGNNNNN
   61 NTCTATATNA GCAGAGCTGG NNNNNNGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA
  121 CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG
  181 ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT
  241 ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA
  301 CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA
  361 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT
  421 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC
  481 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC
  541 ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA
  601 ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG
  661 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACANCC
  721 ACTACCTGAG CACCCNGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG
  781 NCCTGCTGGA NTTCGTGACC GCCGCCGGGA TCNCTCTCGG CATGGACNAG CTGTACAAGT
  841 CCGGANTCAG ATNCATGGCG GGCGANGGAC TNNNGGCNAC NGGGCGCGNC NGNCCACCGC
  901 GGCGCCNGNN ANCCNANNNC NNCTCTTTAA NGNGCTGGNC ATCNGNCGAN NTCGGCGTGG
  961 GTAAGANNAN NATCATCNNG CGNTACGTGN NCCAGCNNNT NNTNCNAGCA CTNNNNNCNN
1021 NNNCNNNNNN NNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17277_A9Eld.seq check
>D06280G5_A9El_1_SV40pro_F_V2_D01_10_ABI24.ab1
    1 NNNNNNNNNT TNNNGNNGNN NNNGCTTTTG CAAGNNCGAT CAAGAGACAG GATGAGGNTC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GANCGGGNNT CTGGGGTTCG AAATGANNGA NNNAGCGNCG
  901 CCCAACCNGN CATCACGAGN NTTCGANNNC NCCGCCNCCT NCTATGAAAG GNTGGGCTTC
961 NNAATCGTTT NCCNNNNNNN NNGNNNGNNT GANNCNTNNN NCGNNGGNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]