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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab27A (#RDB17273)

Expression vector of mouse Rab27A fused with EGFP.

Clone info. Expression vector of mouse Rab27A fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab27a cDNA
Reference of insert sequence AB232622 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al. (2002) J. Biol. Chem. 277, 9212-9218); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。

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RDB17273 pEGFP-C1-Rab27A DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab27A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17273).

Reference section:

Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17273_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5'
Sequence file: RDB17273_A9Ela.seq check
>D06280G1_A9El_2_EGFP-C2_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNCNCGNNCN CNTGGTCCTG CTGGNNTTCG TGNCCGCCGC CGGGNTCACT
   61 CTCGGCATGN NNGAGCTGTA CAAGTCCGGA CTCAGATCCA TGTCGGATGG AGATTACGAT
  121 TACCTCATCA AGTTCTTGGC CTTGGGAGAC TCTGGGGTAG GGAAGACCAG TGTACTCTAC
  181 CAGTACACTG ATGGCAAGTT CAACTCCAAA TTCATCACCA CAGTGGGCAT TGATTTCAGG
  241 GAAAAGAGAG TGGTGTACAG AGCCAATGGG CCAGATGGAG CTGTGGGCCG AGGCCAGAGA
  301 ATCCACCTGC AGTTATGGGA CACGGCGGGG CAGGAGAGGT TTCGGAGCTT AACCACTGCA
  361 TTCTTCAGGG ACGCTATGGG TTTCCTGCTT CTGTTCGACC TGACAAATGA GCAAAGTTTC
  421 CTCAATGTCC GAAACTGGAT AAGCCAGCTA CAGATGCACG CGTACTGTGA AAACCCAGAT
  481 ATAGTGCTGT GTGGAAATAA GAGTGACCTG GAAGACCAGA GGGCAGTGAA AGAGGAGGAG
  541 GCCCGGGAAC TTGCCGAGAA GTACGGAATC CCCTATTTTG AAACCAGTGC TGCCAACGGG
  601 ACAAACATAA GCCACGCGAT TGAGATGCTC CTGGACCTGA TCATGAAGCG GATGGAGCGG
  661 TGTGTGGACA AGTCCTGGAT TCCGGANGGA GTGGTACGGT CCNACGGCCA TACCTCTGCG
  721 GATCAGCTAA GTGANGAGAN NGAGAANGGG TTGTGTGGCT GTTGAAATCA CTAGTGAANT
  781 CTGCAGTCGA NNGTACNGCG GGCCCNGGNT CCACCGGATC TAGATAACTG ATCATAATCN
  841 GCNNTACCNN ATTTGTAGAN GNTTTACNNG CTTTNAAAAA ACCTCCNNCN CNNNCNNNNN
901 NNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17273_A9Elb.seq check
>D06280G1_A9El_2_SV40polyA-R2_D03_12_ABI24.ab1
    1 NNNNNNNNNN CNNCANNNNN AGTNAANNAC AANNATTGCA TTCNTTTTAT GTTTCNGGTT
   61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
  121 GATTATGATC AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACTGCAGA
  181 ATTCACTAGT GATTTCAACA GCCACACAAC CCCTTCTCCT TCTCCTCACT TAGCTGATCC
  241 GCAGAGGTAT GGCCGTTGGA CCGTACCACT CCCTCCGGAA TCCAGGACTT GTCCACACAC
  301 CGCTCCATCC GCTTCATGAT CAGGTCCAGG AGCATCTCAA TCGCGTGGCT TATGTTTGTC
  361 CCGTTGGCAG CACTGGTTTC AAAATAGGGG ATTCCGTACT TCTCGGCAAG TTCCCGGGCC
  421 TCCTCCTCTT TCACTGCCCT CTGGTCTTCC AGGTCACTCT TATTTCCACA CAGCACTATA
  481 TCTGGGTTTT CACAGTACGC GTGCATCTGT AGCTGGCTTA TCCAGTTTCG GACATTGAGG
  541 AAACTTTGCT CATTTGTCAG GTCGAACAGA AGCAGGAAAC CCATAGCGTC CCTGAAGAAT
  601 GCAGTGGTTA AGCTCCGAAA CCTCTCCTGC CCCGCCGTGT CCCATAACTG CAGGTGGATT
  661 CTCTGGCCTC GGCCCACAGC TCCATCTGGC CCATTGGCTC TGTACACCAC TCTCTTTTCC
  721 CTGAAATCAA TGCCCACTGT GGTGATGAAT TTGGAGTTGA ACTTGCCATC AGTGTACTGG
  781 TAGAGTACAC TGGTCTTCCC TANCCCAGAN TCTCCCAANG CCNAGAACTT GATGANGTAA
  841 TCGNAATCTC CATCCNANAT GGNTCTGANT NCGGANTNNT ACAGCTCGNN CATGCCNANA
  901 NNGATCCNNN NNNNNGNCNN NAACTNNNNC NNNNNNNTNN GNTNNCNCNN CNNNNNNGGN
961 NCNTTGNTCN NNNNNNNNN
//
Primer: CMV_Forward
Sequence file: RDB17273_A9Elc.seq check
>D06280G1_A9El_2_CMV_Forward_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNTTNNN GCNNTGGGCG GTANGCGTGT ACGGTGGGNN GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
  121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
  241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
  361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
  421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
  541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
  601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
  661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN
  721 CACCCAGTCC GCCCTGANCA AAGANCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
  781 NTTCGTGACC GCCGCCGGGN TCACTCTCGG CATGGACGAN CTGTACAANT CCGGANTCAG
  841 NNNCATGTCG GANGGANANT ACNANTACNN CATCNNNTTC TNNGNNNTNG GANANTCNNG
901 GGGTNNGNNA GNNCANNNNN NTCNANNANN NNNNNNNN
//
Primer: SV40pro_F_V2
Sequence file: RDB17273_A9Eld.seq check
>D06280G1_A9El_2_SV40pro_F_V2_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCTTTTNN NNNNNCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAN
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG ANCGGGANTC TGGGGTTCGA AATGANCGAN CAAGCGACGC
  901 CCAACCTGNN NTCNCGAGAT TTCNANNNCN CCGCCGCCTT CTATGAAANN NNTGGNNTTC
  961 NNANNNNTTT NNCNNGNANN NCNNNNTGGN TGNNNNTNNN NCGCNGGGNN NNNNNGCTGG
1021 NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]